Sampling Events Research Articles

A Proteogenomic Pipeline for the Analysis of Protein Biosynthesis Errors in the Human Pathogen Candida albicans

Candida albicans is a diploid pathogen known for its ability to live as a commensal fungus in healthy individuals but causing both superficial infections and disseminated candidiasis in immunocompromised patients where it is associated with high morbidity and mortality. Its success in colonizing the human host is attributed to a wide range of virulence traits that modulate interactions between the host and the pathogen, such as optimal growth rate at 37°C, the ability to switch between yeast and hyphal forms, and a remarkable genomic and phenotypic plasticity. A fascinating aspect of its biology is a prominent heterogeneous proteome that arises from frequent genomic rearrangements, high allelic variation, and high levels of amino acid misincorporations in proteins. This leads to increased morphological and physiological phenotypic diversity of high adaptive potential, but the scope of such protein mistranslation is poorly understood due to technical difficulties in detecting and quantifying amino acid misincorporation events in complex protein samples. We have developed and optimized mass spectrometry and bioinformatics pipelines capable of identifying rare amino acid misincorporation events at the proteome level. We have also analyzed the proteomic profile of an engineered C.albicans strain that exhibits high level of leucine misincorporation at protein CUG sites and employed an invivo quantitative gain-of-function fluorescence reporter system to validate our LC-MS/MS data. C.albicans misincorporates amino acids above the background level at protein sites of diverse codons, particularly at CUG, confirming our previous data on the quantification of leucine incorporation at single CUG sites of recombinant reporter proteins, but increasing misincorporation of Leucine at these sites does not alter the translational fidelity of the other codons. These findings indicate that the C.albicans statistical proteome exceeds prior estimates, suggesting that its highly plastic phenome may also be modulated by environmental factors due to translational ambiguity.

Spatiotemporal heterogeneity of lake carbon dioxide flux leads to substantial uncertainties in regional upscaling estimates

Limited field samplings result in significant uncertainties in regional and global estimates of lake carbon dioxide (CO2) emissions. However, quantitative analysis of uncertainty in regional lake CO2 emission estimates remains unclear. In this study, we utilized satellite data to estimate carbon dioxide flux from 113 eastern China lakes, revealing substantial spatial and temporal variations in flux, averaging 18.07 ± 81.83 mg m−2 d−1. Additionally, satellite-estimated total CO2 effluxes indicated previous upscaling studies had overestimated total CO2 effluxes from these studied lakes by approximately 3–11 times, primarily due to substantial variations in lake CO2 fluxes. Insufficient sampling resolution resulted in considerable uncertainty in upscaling estimations. Temporal variations in carbon dioxide contributed greater upscaling uncertainties than spatial variations in carbon dioxide. To capture the dynamics of lake CO2, increasing the number of sampling points and events is necessary as lake size decreases and trophic state increases. Finally, we propose a prediction for the optimal sampling resolution based on lake area and trophic state, recommending an average of 5 points per lake and bi-monthly sampling as the ideal resolution for similar shallow eutrophic lakes. This approach has been validated as effective in lakes across North America and Europe. We believe that future global-scale lake carbon budget estimates would benefit from field observations conducted at more reasonable sampling points and frequency.

EIF1 and eIF5 dynamically control translation start site fidelity.

Translation initiation defines the identity of a synthesized protein through selection of a translation start site on a messenger RNA. This process is essential to well-controlled protein synthesis, modulated by stress responses, and dysregulated in many human diseases. The eukaryotic initiation factors eIF1 and eIF5 interact with the initiator methionyl-tRNAi Met on the 40S ribosomal subunit to coordinate start site selection. Here, using single-molecule analysis of in vitro reconstituted human initiation combined with translation assays in cells, we examine eIF1 and eIF5 function. During translation initiation on a panel of RNAs, we monitored both proteins directly and in real time using single-molecule fluorescence. As expected, eIF1 loaded onto mRNAs as a component of the 43S initiation complex. Rapid (~ 2 s) eIF1 departure required a translation start site and was delayed by alternative start sites and a longer 5' untranslated region (5'UTR). After its initial departure, eIF1 rapidly and transiently sampled initiation complexes, with more prolonged sampling events on alternative start sites. By contrast, eIF5 only transiently bound initiation complexes late in initiation immediately prior to association of eIF5B, which allowed joining of the 60S ribosomal subunit. eIF5 association required the presence of a translation start site and was inhibited and destabilized by alternative start sites. Using both knockdown and overexpression experiments in human cells, we validated that eIF1 and eIF5 have opposing roles during initiation. Collectively, our findings demonstrate how multiple eIF1 and eIF5 binding events control start-site selection fidelity throughout initiation, which is tuned in response to changes in the levels of both proteins.

Monitoring drift and associated biodiversity of nearshore rafts of holopelagic Sargassum spp. in the Mexican Caribbean

Recent recurrent inundations of holopelagic Sargassum species (sargasso) in the tropical North Atlantic warrant studies to increase our understanding of this phenomenon. We carried out a multidisciplinary study to determine the drift and associated biota of sargasso rafts in nearshore waters of a reef system in the Mexican Caribbean, during the 2020–2022 influx seasons. Home-designed trackable drifting buoys revealed notorious influence of winds stronger than 4–5 m s−1 on the rafts´ displacement. At lower wind velocities, the drift was almost entirely determined by currents; local topography played a role in concentrating or dispersing the algae. Video cameras attached to the drifting buoys allowed for non-invasive identification of free-swimming ichthyofauna, recording low specific richness with a dominance of species of the Carangidae family. Sargasso associated biota was sampled with a dipnet. The small motile macrofauna was abundant and diverse, with a dominance of few species. Epibionts (epifauna and epiphytic algae) showed low specific diversity, while the microbial communities were dominated by (potentially pathogenic) Vibro spp. Small motile macrofauna was the only biota group with new records for the study region. Differences among sampling events were observed in the associated community; suggesting large inherent variability of the rafts´ biota. The information on wind, coastal hydrodynamics and topography on the displacement of the sargasso rafts will allow for better predictions of strandings, and information on associated non-native biota or dominant microbial species helped identify groups that should be closely monitored; thereby aiding in targeted management actions for mitigating the impacts of the inundations.

Temporal decreases in pathogen colonization and infection among hospitalized neonates following routine skin antisepsis with chlorhexidine gluconate: Botswana 2022 – 2023

Background: Multidrug-resistant Gram-negative bacteria are a major cause of sepsis among hospitalized neonates globally. Aqueous chlorhexidine gluconate (CHG) skin antisepsis has been shown to be safe for use in infants; however, its sustained effectiveness in preventing Gram-negative pathogen colonization, bloodstream infection (BSI), and mortality is unclear. Methods: We conducted a period prevalence survey, with 26 sampling events over 12 months (18 October 2022 – 31 October 2023) at a 33-bed neonatal unit in a tertiary public hospital in Botswana where ESBL-producing Klebsiella pneumoniae and carbapenem-resistant Acinetobacter baumannii are leading causes of BSI. Perirectal and periumbilical skin swabs were collected every two weeks from all inpatients. Swabs were inoculated onto chromogenic media selective and differential for extended-spectrum beta-lactamase producing Enterobacterales (ESBL-E) and Acinetobacter spp. (CHROMagar™ ESBL, Acinetobacter). Colonization status was determined based on culture growth and colony morphology. Contemporaneous data on all-cause mortality and BSI were abstracted from routine surveillance records. Pre- and post-CHG prevalences were compared using a simple Chi-square test. During the surveillance period, an outbreak of K. pneumoniae linked to contaminated multi-use vials was detected, thus BSIs and deaths during the outbreak period (2 February–6 April, 2023) were excluded. In February 2023, the hospital infection prevention and control (IPC) team introduced twice-weekly whole-body cleansing with commercially available 2% aqueous CHG, performed by caregivers and healthcare workers on neonates >24 hours old and weighing ≥1 kg until discharge. Results: There were significant decreases in ESBL-E and Acinetobacter skin and perirectal colonization following the CHG intervention (Table 1; Figure 1). After the CHG intervention, the incidence of Acinetobacter BSIs declined significantly and there was a trend toward a decline in other BSIs and mortality. No adverse events associated with CHG were reported. Conclusions: Twice-weekly CHG application was temporally associated with significant reductions in neonatal ESBL-E and Actinetobacter skin and perirectal colonization and Acinetobacter BSI. This analysis was limited by a short pre-intervention surveillance period and thus may have been influenced by confounders such as seasonality, and intensified IPC efforts following the outbreak. Analysis of the routine CHG use in other settings and over longer surveillance periods are needed to better understand its effectiveness as an IPC strategy in settings where neonatal sepsis incidence is high. Table 1. Colonization prevalence, BSI incidence, and mortality surrounding introduction of CHG skin cleansing in a neonatal unit, 18 October 2022 – 31 October 2023.

Red Sea Coral Reef Monitoring Site in Sudan after 39 Years Reveals Stagnant Reef Growth, Continuity and Change

Coral reefs off the coast of the Republic of Sudan are still considered to be among the most pristine reefs in the central Red Sea. The complex coastal fringing reefs, offshore banks, and shoals of Dungonab Bay in the north and Sanganeb atoll situated further to the south, about 23 km off the Sudanese mainland coast, were inscribed on the UNESCO World Heritage List in 2016. Due to their remote location and limited access, monitoring of the status of the reefs has been sporadic. Here, we present the results of a repeated large area photomosaic survey (5 m × 5 m plots) on the Sanganeb atoll, first established and surveyed in 1980, and revisited in 1991 and most recently in 2019. The 2019 survey recovered and reinstated the four original monitoring plots. Evaluation of photographic and video records from one photomosaic plot on the seaward slope of the atoll revealed general continuity of the overall community structure and composition over 39 years. Individual colonies of Echinopora gemmacea and Lobophyllia erythraea were recorded in the exact same positions as in the 1980 and 1991 plots. The genera Acropora and Pocillopora remain dominant, although in altered proportions. Shifts in composition were detected at the species level (e.g., increase in Pocillopora verrucosa, Stylophora pistillata, Acropora hemprichii, Dipsastraea pallida, and Echinopora gemmacea, decrease in Acropora cytherea and A. superba), in addition to changes in the extent of uncolonized substrate (e.g., increase from 43.9% in 1980 to 52.2% in 2019), and other scleractinian, hydrozoan, and soft coral living cover. While the temporal resolution only includes three sampling events over 39 years (1980, 1991, 2019), this study presents one of the longest time series of benthic community surveys available for the entire Red Sea. A semi-quantitative estimate of vertical reef growth in the studied test plot indicates a reduction in net accretion rates of more than 80%, from 2.27 to 2.72 cm/yr between 1980 and 1991 to 0.28–0.42 cm/yr between 1991 and 2019. We carefully conclude that the changes observed in the coral community in the plot in 2019 (Acropora–Pocillopora shift, increase in Montipora and calcareous algae) are representative of impacts at the community level, including rising sea surface temperatures and recent bleaching events.

When Bitcoin is high: cryptocurrency value, illicit markets and US marijuana bills

PurposeThe purpose of this paper is to estimate the implications of illicit market use for the value of Bitcoin in an event studies framework.Design/methodology/approachThis study uses a data set of 58 state-level marijuana decriminalisation and legalisation bills and referenda in the USA in 2010–2022.FindingsDecriminalisation is associated with a strong and consistent positive Bitcoin price response around the event, recreational legalisation induces a more ambiguous reaction and medical legalisation is found to have a negative albeit small impact on Bitcoin value. This suggests decriminalisation enhances shadow economy use value of Bitcoin, whereas recreational and medical legalisation are not consistently reducing illicit drug cryptomarket activity. The effects are robust to various estimation windows, in subsamples, and also when outliers, heavy tails, conditional heteroskedasticity and state size are accounted for.Originality/valueNew to the literature, the choice of US marijuana bills, specifically as sample events, is based on both theoretical and empirical grounds.

Distributed periodic event-triggered secondary control for islanded microgrids with switching topologies and communication delays

With the application of distributed generators (DGs), microgrids (MGs) have gained much attention. Combined with the distributed communication of networked systems, we investigates the secondary restoration problem in islanded microgrids. The periodic event-triggered (PET) controllers are designed for voltage restoration and frequency restoration with accurate active power allocation, which can naturally avoid Zeno behavior. By means of a common Lyapunov function, a PET protocol is designed that requires only the information of neighbors for event detection. Both event-checking sampling period and event trigger parameter are taken into account to reduce computation and communication redundancy by choosing appropriate parameters. Furthermore, considering the effect of communication delay, sufficient conditions are given to accomplish the secondary control objectives with allowable delays. Switching topologies are considered in an extra discussion. Finally, the effectiveness of the proposed PET control strategies is verified by several MATLAB simulations.

Detection of genetically modified organisms using highly multiplexed amplicon sequencing

The circulation of products based on genetically modified (GM) organisms is highly regulated by some governments with strict implementation rules for the breeding, planting, marketing, labelling, and trading of such products. To ensure compliance, accurate detection methods for GM events are necessary, along with assurance that GM material falls within relevant threshold levels. The increasing complexity and potential of undocumented GM are a growing challenge for genetic screening. Here, we developed and assessed a highly multiplexed amplicon sequencing assay for the detection of GM events based on a microfluidics platform and next-generation sequencing (NGS). To probe GM events comprehensively, we designed a total of 230 new amplicons to cover flanking, promoter, junction, and coding sequences of GM sequences. In addition, we designed and implemented parallel amplification of ribosomal and chloroplast markers to define crop species identity from potentially mixed samples. Using reference GM material of 11 crop species and multiple amplicons, we successfully detected the presence of 10 known modifications per GM event. We also find that reported flanking sequences of GM events may not be all useful for diagnostic. We assessed the assay's potential to detect GM events in mixed samples as well as in highly diluted DNA. Finally, we performed a prospective search of potentially undocumented GM events in plant material. Our microfluidics-based amplicon GM detection approach fills important gaps in detecting potentially undocumented and complex GM events by recovering a wide range of specific amplicon sequences for evaluation. Integrating highly parallel amplicon assays in GM screening efforts should be an effective complement to aid post-market monitoring and regulatory compliance efforts.

Subtype-specific alternative splicing events in breast cancer identified by large-scale data analysis

Genome analysis in cancer has focused mainly on elucidating the function and regulatory mechanisms of genes that exhibit differential expression or mutation in cancer samples compared to normal samples. Recently, transcriptome analysis revealed that abnormal splicing events in cancer samples could contribute to cancer pathogenesis. Moreover, splicing variants in cancer reportedly generate diverse cancer antigens. Although abnormal splicing events are expected to be potential targets in cancer immunotherapy, the exploration of such targets and their biological significance in cancer have not been fully understood. In this study, to explore subtype-specific alternative splicing events, we conducted a comprehensive analysis of splicing events for each breast cancer subtype using large-scale splicing data derived from The Cancer Genome Atlas and found subtype-specific alternative splicing patterns. Analyses indicated that genes that produce subtype-specific alternative splicing events are potential novel targets for immunotherapy against breast cancer. The subtype-specific alternative splicing events identified in this study, which were not identified by mutation or differential expression analysis, bring new significance to previously overlooked splicing events.

Impacts of Millipedes on Acari and Collembola Communities-A Microcosm Experiment.

Ecosystem engineers influence the structure and function of soil food webs through non-trophic interactions. The activity of large soil animals, such as earthworms, has a significant impact on the soil microarthropod community. However, the influence of millipedes on soil microarthropod communities remains largely unknown. In this microcosm experiment, we examined the effects of adding, removing, and restricting millipede activity on Acari and Collembola communities in litter and soil by conducting two destructive sampling sessions on days 10 and 30, respectively. At the time of the first sampling event (10 d), Acari and Collembola abundance was shown to increase and the alpha diversity went higher in the treatments with millipedes. At the time of the second sampling event (30 d), millipedes significantly reduced the Collembola abundance and alpha diversity. The results were even more pronounced as the millipedes moved through the soil, which caused the collembolans to be more inclined to inhabit the litter, which in turn resulted in the increase in the abundance and diversity of Acari in the soil. The rapid growth of Collembola in the absence of millipedes significantly inhibited the abundance of Acari. The presence of millipedes altered the community structure of Acari and Collembola, leading to a stronger correlation between the two communities. Changes in these communities were driven by the dominant taxa of Acari and Collembola. These findings suggest that millipedes, as key ecosystem engineers, have varying impacts on different soil microarthropods. This study enhances our understanding of biological interactions and offers a theoretical foundation for soil biodiversity conservation.

The Study of SRSF1 Regulates Abnormal Alternative Splicing of BCL2L11 and the Role in Refractory Acute Myeloid Leukemia.

Abnormalities in splicing factors, such as mutations or deregulated expression, can lead to aberrant splicing of target genes, potentially contributing to the pathogenesis of acute myeloid leukemia (AML). Despite this, the precise mechanism underlying the abnormal alternative splicing (AS) induced by SRSF1, a splicing factor associated with poor AML prognosis, remains elusive. Using strict splicing criteria, we globally screened for AS events in NPMc-positive and NPMc-negative AML samples from TCGA. An AS network associated with AML prognosis was then established. Functional assays, including CCK-8, flow cytometry, and Western blot, were conducted on K562 and THP-1 cells overexpressing SRSF1. Cell viability following 72-h Omipalisib treatment was also assessed. To explore the mechanism of SRSF1-induced AS, we created a BCL2L11 miniGene with a site-specific mutation at its branch point. The AS patterns of both wild-type and mutant miniGenes were analyzed following SRSF1 overexpression in HEK-293T, along with the subcellular localization of different spliceosomes. SRSF1 was significantly associated with AML prognosis. Notably, its expression was markedly upregulated in refractory AML patients compared to those with a favorable chemotherapy response. Overexpression of SRSF1 promoted THP-1 cell proliferation, suppressed apoptosis, and reduced sensitivity to Omipalisib. Mechanistically, SRSF1 recognized an aberrant branch point within the BCL2L11 intron, promoting the inclusion of a cryptic exon 3, which in turn led to apoptosis arrest. Overexpression of SRSF1 and the resulting abnormal splicing of BCL2L11 are associated with drug resistance and poor prognosis in AML.

Can the Culture Density of the Marine Copepod Pseudodiaptomus nihonkaiensis be Improved by Installing an Artificial Substrate?

The understanding of the increasing population density of copepods in culture tanks contributes to establish their stable cultures at high productions for aquaculture industry, which are preferred live feeds for fish larvae. In the present study, the semi-benthic copepod Pseudodiaptomus nihonkaiensis were used as target species. This genus is often found from brackish water and its tolerance to a wide range of salinity and water quality has attracted attention as a potential commercial live feed for aquaculture. Population growth and egg production in the semi-benthic copepod P. nihonkaiensis are investigated. Copepods were cultured in tanks without mesh, with mesh lining tank walls, and with 2, 4 or 8 mesh partitions in each tank to identify the effect of substratum surface area on copepod population density. Linear regression revealed peaks in nauplius, copepodite, adult and total densities, and the cumulative number of eggs for all sampling events to correlate positively with substratum surface area (p<0.01). Greater substratum area within a culture tank may improve egg production by increasing habitat for ovigerous females. The simple installation of inexpensive artificial substrata in existing copepod culture tanks might improve the feasibility of using copepods as a live food in commercial-scale aquaculture.

Sulfur source effects on soil, vegetation indices, biomass, and uptake in potato

AbstractSulfur (S) is necessary for amino acid production, disease resistance, protein synthesis, and nitrogen assimilation in plants. We tested the performance of three S sources (magnesium sulfate [EPTOP], gypsum, and ammonium sulfate [AS]) at two different rates (45 and 90 kg S ha−1) on soil S availability, vegetative indices (VIs), above‐ and belowground biomass (BGB), and potato (Solanum tuberosum L.) S uptake. For VIs such as normalized difference vegetative index, normalized difference red‐edge index, and chlorophyll content, the maximum values were reported when gypsum and EPTOP were applied compared to AS. These differences were decreased later in the season. Significant interaction effects were reported among sites, sampling events, and sites and S sources for aboveground biomass (AGB). Similar trends were reported for the BGB, where the significant interaction effect was reported among the S sources and sampling event and S sources and sites. The AGB S uptake was affected due to the sites and sampling event. However, BGB S uptake was influenced by the interactive effect of S sources, sites, and sampling events. The VIs did not have a significant association between soil S concentration and AGB S uptake. It was found that the field history had a notable effect on the crop responses to variable S sources. In conclusion, applying S rates and sources has distinct responses, which depend on soil history and chemical properties, and that should be considered when making choices for S application in potatoes.

Detection of HBV DNA integration in plasma cell-free DNA of different HBV diseases utilizing DNA capture strategy

The landscape of hepatitis B virus (HBV) integration in the plasma cell-free DNA (cfDNA) of HBV-infected patients with different stages of liver diseases [chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC)] remains unclear. In this study, we developed an improved strategy for detecting HBV DNA integration in plasma cfDNA, based on DNA probe capture and next-generation sequencing. Using this optimized strategy, we successfully detected HBV integration events in chimeric artificial DNA samples and HBV-infected HepG2-NTCP cells at day one post infection, with high sensitivity and accuracy. The characteristics of HBV integration events in the HBV-infected HepG2-NTCP cells and plasma cfDNA from HBV-infected individuals (CHB, LC, and HCC) were further investigated. A total of 112 and 333 integration breakpoints were detected in the HepG2-NTCP cells and 22 out of 25 (88%) clinical HBV-infected samples, respectively. In vivo analysis showed that the normalized number of support unique sequences (nnsus) in HCC was significantly higher than in CHB or LC patients (P values ​< ​0.05). All integration breakpoints are randomly distributed on human chromosomes and are enriched in the HBV genome around nt 1800. The majority of integration breakpoints (61.86%) are located in the gene-coding region. Both non-homologous end-joining (NHEJ) and microhomology-mediated end-joining (MMEJ) interactions occurred during HBV integration across the three different stages of liver diseases. Our study provides evidence that HBV DNA integration can be detected in the plasma cfDNA of HBV-infected patients, including those with CHB, LC, or HCC, using this optimized strategy.

Comparison of chemical contaminant measurements using CLAM, POCIS, and silicone band samplers in estuarine mesocosms.

Discrete water samples represent a snapshot of conditions at a particular moment in time and may not represent a true chemical exposure caused by changes in chemical input, tide, flow, and precipitation. Sampling technologies have been engineered to better estimate time-weighted concentrations. In this study, we consider the utility of three integrative sampling platforms: polar organic chemical integrative sampler (POCIS), silicone bands (SBs), and continuous, low-level aquatic monitoring (CLAM). This experiment used simulated southeastern salt marsh mesocosm systems to evaluate the response of passive (POCIS, SBs) and active sampling (CLAM) devices along with discrete sampling methodologies. Three systems were assigned to each passive sampler technology. Initially, all tanks were dosed at nominal (low) bifenthrin, pyrene, and triclosan concentrations of 0.02, 2.2, and 100 µg/L, respectively. After 28 days, the same treatment systems were dosed a second time (high) with bifenthrin, pyrene, and triclosan at 0.08, 8.8, and 200 µg/L, respectively. For passive samplers, estimated water concentrations were calculated using published or laboratory-derived sampling rate constants. Chemical residues measured from SBs resulted in high/low ratios of approximately 2x, approximately 3x, and 1x for bifenthrin, pyrene, and triclosan. A similar pattern was calculated using data from POCIS samples (~4x, ~3x, ~1x). Results from this study will help users of CLAM, POCIS, and SB data to better evaluate water concentrations from sampling events that are integrated across time. Integr Environ Assess Manag 2024;20:1384-1395. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Atmospheric concentrations and potential sources of dioxin-like contaminants to Acadia National Park

Acadia National Park (ANP) is located on Mt. Desert Island, ME on the U.S. Atlantic coast. ANP is routinely a top-ten most popular National Park with over four million visits in 2022. The overall contribution and negative effects of long-range atmospheric transport and local sources of dioxin-like contaminants endangering natural and wildlife resources is unknown. Dioxin-like (DL) contaminants polychlorinated dibenzo-p-dioxins (∑PCDD) and polychlorinated dibenzofurans (∑PCDF), non-ortho coplanar PCBs (∑CP4), and polychlorinated naphthalenes (∑PCNs) were measured at the McFarland Hill air monitoring station (44.37⁰N, 68.26⁰W). On a mass/volume basis, total PCNs averaged 90.9 % (788 fg/m3) of DL contaminants measured annually, with 92.9 % of the collected total in the vapor-phase. Alternatively, total dioxin/furans (∑PCDD/Fs) represented 71.6 % of the total toxic equivalence (∑TEQ) (1.018 fg-TEQ/m3), with 69.7 % in the particulate-phase. Maximum concentrations measured for individual sampling events for ∑PCDD/F, ∑CP4, and ∑PCN were 159 (winter), 139 (summer), and 2100 (autumn), fg/m3 respectively. Whereas the maximum ∑TEQ concentrations for individual sampling events for ∑PCDD/F, ∑CP4, and ∑PCN were 2.8 (autumn), 0.38 (summer), and 0.71 (autumn), fg-TEQ/m3 respectively. Pearson correlations were calculated for ∑PCDD/Fs and ∑PCN particulate/vapor-phase air concentrations and PM2.5 wood smoke “indicator” species. The most significant correlations were observed in autumn for particulate-phase ∑PCDD/Fs suggesting a relationship between visitation-generated combustion sources (campfires and/or waste burning) or climate-change mediated forest fires. Significant Clausius-Clapeyron (C–C) correlations observed for particulate-phase ∑PCDDs (r2 = 0.567) as ambient temperatures decreased suggests a connection between localized domestic heating sources or visitor-based burning of wood/trash resources. Alternatively, highly significant C–C vapor-phase ∑CP4-PCBs correlations (r2 = 0.815) implies that the majority of ∑CP4-PCB loading to ANP is from long-range atmospheric transport processes. Based on these findings, Acadia National Park should be classified as a remote site with minor depositional impacts from ∑PCDD/Fs, ∑CP4-PCBs, and ∑PCN atmospheric transport or local diffuse sources.

Deliberately introduced dung beetles in Australia: 12 years of occurrence and abundance records from 2001 to 2022.

Since 1968, the Australian Dung Beetle Project has carried out field releases of 43 deliberately introduced dung beetle species for the biological control of livestock dung and dung-breeding pests. Of these, 23 species are known to have become established. For most of these species, sufficient time has elapsed for population expansion to fill the extent of their potential geographic range through both natural and human-assisted dispersal. Consequently, over the last 20 years, extensive efforts have been made to quantify the current distribution of these introduced dung beetles, as well as the seasonal and spatial variation in their activity levels. Much of these data and their associated metadata have remained unpublished, and they have not previously been synthesized into a cohesive dataset. Here, we collate and report data from the three largest dung beetle monitoring projects from 2001 to 2022. Together, these projects encompass data collected from across Australia, and include records for all 23 species of established dung beetles introduced for biocontrol purposes. In total, these data include 22,718 presence records and 213,538 absence records collected during 10,272 sampling events at 546 locations. Most presence records (97%) include abundance data. In total, 1,752,807 dung beetles were identified as part of these data. The distributional occurrence and abundance data can be used to explore questions such as factors influencing dung beetle species distributions, dung beetle biocontrol, and insect-mediated ecosystem services. These data are provided under a CC-BY-NC 4.0 license and users are encouraged to cite this data paper when using the data.

Aquatic macroinvertebrate community and water quality in River Tambo, Arequipa region, Peru

Introduction: The River Tambo basin is one of the main hydrographic systems of the Peruvian western watershed, being a source of development for agriculture, agroindustry, livestock, and domestic use in the Tambo Valley and Arequipa City. Objective: To analyze the structure of macroinvertebrates in River Tambo, Perú and assess the water quality through this community. Methods: Five sampling events were conducted between December 2018 and November 2019 in 12 stations, using a Surber net (500 μm). Macroinvertebrates were identified at a family level. To assess community structure, richness, Simpson’s dominance index (D), Pielou’s evenness (J’), and true diversity of order 1 were determined. A nMDS based on the Bray-Curtis index was used to evaluate dissimilarity. To check for differences in community structure, ANOSIM and two-way MANOVA were used. SIMPER was used to establish family contribution to sample similarity. Relations between physicochemical and biological variables were determined by CCA. ABI index was applied to assess ecological quality. Results: 32 families were recognized, being the most abundant: Baetidae, Chironomidae, Leptohyphidae, Simuliidae, Hydroptilidae, and Elmidae. The middle and low zones of the basin showed the highest and lowest diversity respectively. There were significant differences (P &lt; 0.05) in the community structure indices among the 12 stations differed. There was a higher similarity among the 12 stations in December. ABI scores increased from the low to the middle part of the river, which showed the highest ecological quality. Conclusion: Macroinvertebrate communities from River Tambo reflect ecosystem conditions. Environments impacted by human activities show lower diversity and ecological quality, due to the structure, habitat, and water quality of the river being altered.

Environmental DNA metabarcoding reveals fish diversity, community assembly and one invasive species prevalence in a National Park of Liaohe in September

The escalating environmental changes are imposing multiple pressures on biodiversity conservation, thereby, more attention has been paid to fish diversity and community assembly patterns. Fish diversity in Liaohe River has deteriorated significantly due to overfishing, environmental pollution and other reasons, in addition, no fish survey related research has been published in Liaohe National Park in recent years. In order to study the diversity of fish species in the area and to investigate the presence of any invasive species. This study used eDNA metabarcoding to explore the distribution and diversity of fish communities in the Liaohe National Park. The study showed that a total of 54 species of fish from six orders, 14 families, and 39 genera were detected, meantime, the invasive species Lepomis cyanellus was found at two sampling sites. The α-diversity results showed that estuarine sites had the highest species diversity. The spatial structure of fish communities in the upstream and downstream reaches were similar as indicated by non-metric multidimensional scaling. In our study, the fish community is influenced by both deterministic and stochastic processes, with the stochastic processes being the main factor. The potential existence of L. cyanellus in Liaohe National Park might be resulted from aquaculture, ornamental pet trade, other economic fish farming, artificial release. The relatively low number of fish species in this study may be attributed to the limited number of sampling events and sampling sites. In addition to these factors, over exploitation of resources, water pollution, and the invasion of alien species have directly impacted the biodiversity of aquatic life. The results of this study lay a foundation for the future biodiversity investigation in National Parks of China, meantime, the use of eDNA monitoring is crucial to prevent, to control and limit the spreading of invasive species in advance.