Fresh Vegetable Samples Research Articles

Photosynthate, heavy metals contents, biochemical characteristics and anatomical responses in Telfairia occidentalis. and Amaranthus hybridus collected within akure metropolis

Heavy metals in soils pose a potential threat to the environment and can cause significant damage to human and animal health. This study aimed to assess heavy metal concentrations, photosynthate levels, biochemical characteristics, and anatomical responses in Telfairia occidentalis and Amaranthus hybridus collected within Akure metropolis, Ondo State, Nigeria. Fresh vegetable samples were randomly collected from four fertilized farmlands (denoted as A, B, C, and D) within the Akure metropolis. Heavy metal concentrations in the vegetable samples were determined using an Atomic Absorption Spectrophotometer (AAS). The pigment content (Chlorophyll a and b) of the vegetables was measured, while the activities of Catalase (CAT), Superoxide Dismutase (SOD), and reduced glutathione (GSH) were estimated. Stomatal architecture and epithelial cell structure were also analyzed. Results indicated that CAT (7.45 ± 0.36 U/mg protein) and SOD (6.21 ± 0.28 U/mg protein) activities, as well as GSH content (4.63 ± 0.12 µmol/g tissue), were highest in vegetables collected from farm A, showing statistically significant differences compared to other farms (p < 0.05). The concentrations of heavy metals in the leaves of vegetables collected from all farms were below the standard limits recommended by WHO/FAO, with the exception of Lead (Pb), which was significantly higher than the permissible limit in farm B (2.14 ± 0.09 mg/kg; p < 0.05). Chlorophyll content was significantly reduced in both vegetables compared to the control (chlorophyll a: 2.56 ± 0.21 mg/g tissue in T. occidentalis and 2.03 ± 0.19 mg/g tissue in A. hybridus; p < 0.05). This reduction in chlorophyll content correlated with the elevated levels of Pb in farm B (r = -0.87, p < 0.05). The stomatal architecture analysis revealed significant alterations in stomatal density and pore size in both vegetables from contaminated farms. These findings suggest the need for biomonitoring programs to control heavy metal accumulation in Nigerian farmlands, as elevated levels of Pb can disrupt the physiological and biochemical processes of vegetables, posing potential health risks to consumers.

Occurrence and Diversity of Intestinal Parasites on Raw Vegetables Sold in Samaru, Zaria

Vegetables are part of everyday diet. There has been more emphasis on their palatability and nutritional values, but they potentially contribute to transmitting intestinal parasites, among other pathogens, to man. This study examined 10 samples of five commonly consumed vegetables from Samaru in Zaria, Nigeria. Twenty-five (25g) fresh vegetable samples were washed in 225 mL of sterile normal saline and gently shaken for 60 seconds. The wash water was allowed to stay for 3 hr in the dark. The supernatant was carefully discarded until only about 15mL was left. The sediment was further concentrated by centrifuging at 3000 revolutions per minute for 5 minutes and examined for intestinal parasites on wet mounts using the compound light microscope. The overall occurrence of intestinal parasites on the vegetables was 22.0%. Ascaris lumbricoides was the most occurring parasite (18.0%), followed by equal 2.0% of hookworms, Trichuris trichiura and Fasciola species. Vegetable samples obtained from Mangorori Market had the highest contamination with Ascaris lumbricoides than other markets, but overall parasitic contamination was highest on vegetables obtained from ABU Community Market. Cabbage was the most contaminated (50.0%), followed by 20% each of lettuce and carrots. Samples displayed on mats during the sale had the highest contamination with Ascaris lumbricoides (23.5%), followed by those displayed on the ground (22.2%). There was higher intestinal parasitic contamination of vegetables sold in dirty surroundings (23.3%) than in relatively clean surroundings (10.0%). Sufficient orientation on the role of fresh raw vegetables in transmitting intestinal parasites should be aimed at farmers, sellers, distributors and final consumers to curb potential health risks

A sensitive paper-based vapor-test kit for instant formalin detection in food products

A colorimetric sensing method based on a paper-based vapor-test kit was successfully developed for the selective and sensitive real-time monitoring of formalin in food samples. The device was specifically designed to efficiently extract and detect formalin simultaneously. A microcentrifuge tube was used as the sample solution container, with the inner cap serving as the reaction and detection zone. Formalin was converted into gaseous formaldehyde through controlled heating, which was then extracted and collected on a filter paper coated with Nash's reagent. The color change on paper was used for formalin quantification using a smartphone for detection and image analysis. Under optimal conditions, our method provided a linear range of 0.5–75 mg L−1 with a detection limit of 0.11 mg L−1. This method effectively determined formalin in fresh food and vegetable samples, with recoveries ranging from 92 to 111%, demonstrating comparable accuracy to the standard method for practical food quality control and safety.

Quality investigation of the most consumed refined seed oils after French fries’ domestic short-term deep-frying process

Deep-frying is an attractive food preparation method and has been in use for centuries. It is a very popular culinary practice worldwide and can be used both industrially and domestically. Deep-fried food has a unique sensorial property, such as flavor, aroma, and texture. However, the chemical quality of the frying oil can be affected by the high temperatures normally used in deep-frying. Complex series of reactions that occur in vegetable oils during deep-frying (hydrolytic and oxidative rancidity processes) they are related to several mechanisms, such as reactive species, chemical composition of the lipid, environment, and process, influencing the speed and nature of the oxidative process. This paper aimed to analyze the quality of the vegetable oils most consumed by the population (refined soybean and sunflower oils) used in domestic short-term deep-frying process, using a domestic-grade electric fryer and a conventional method. French fries were used because they are a convenient product, highly consumed in industrialized countries. Domestic events of short-term deep-frying were carried out for three days, with one-week intervals. After each frying events, the refined oils were analyzed. Methodology consists in bromatological analyses of quality research applying analyzes of acidity and peroxide, in which the free acidity content, compounds formed by hydrolytic rancidity, and hydroperoxides content, the first compounds formed in the oil´s oxidative rancidification process, were analyzed. These parameters will be fundamental in oils oxidative evaluating during frying. According to the results, fresh vegetable oils samples remained within regulatory limits. However, after short-term deep-frying, free fatty acids and hydroperoxides content increased in both domestic short-term deep-frying using both vegetable oils, exception of sunflower oil after last day of frying. Additionally, there was no significant difference in the fatty acids value increase comparing with both domestic frying techniques. However, there was significant difference in hydroperoxide value comparing the oils between deep-frying techniques. However, in general even the oils have shown great thermal quality, bromatological analyses demonstrated that must be taken careful when reusing oils for short-term deep-frying. Seeing that the electric fryer is effective in oil quality compared preservation comparing with conventional method, probably due to temperature control and better sealing efficiency.

Parasitic contamination of fresh vegetables and fruits sold in open-air markets in peri-urban areas of Jimma City, Oromia, Ethiopia: A community-based cross-sectional study.

Consuming contaminated raw vegetables and fruits is one of the primary means of parasite transmission to humans. Periodic monitoring of parasitic contamination in these food items is a crucial step in preventing the spread of parasitic disease in the community. This study was aimed at detecting intestinal parasitic contamination caused by consuming raw vegetables and fruits sold in three open-air markets and its associated factors in peri-urban areas of Jimma City, Oromia, Ethiopia. A cross-sectional study was conducted on fruits and vegetables collected from three peri-urban open-aired markets (namely; Hora Gibe, Bore and Jiren markets) in peri-urban areas of Jimma City between July and September 2021. A total of 187 fresh vegetable samples and 188 fruits were collected and examined for intestinal parasite contamination. About 200g of fruit and vegetable samples were processed and examined microscopically for parasite contamination, utilizing direct wet mount and modified Zeihl-Neelson staining methods in accordance with standard protocols. A structured questionnaire was used to collect data on the socio-demographic characteristics of vendors and risk factors for fruit and vegetable contamination. All data were analyzed using SPSS version 20.0. Of the 187 fresh vegetable samples and 188 fresh vegetable samples, 105 (56.1%) and 68/188 (36.2%) of vegetables and fruit samples, respectively, were found contaminated with one or more intestinal parasites. Remarkably, high level of contamination in fresh vegetable samples was recorded both in Carrot (Daucus carota) 63.8% (30/46) and Lettuce (Lactuca sativa) 63.1% (29/46) while Green pepper (Capsicum spp.) is the least contaminated. In fruit samples, Avokado (Persea americana) 42.6% (20/47) and Banana (Musa acuminata) 14.9% (7/47) were the most and the least commonly contaminated items respectively. The identified helminthes and protozoans were Ascaris lumbricoides, Strongyloides stercoralis, Hymenolepis nana, Entamoeba histolytica/dispar, Giardia lamblia, Cryptosporidium spp., Toxocara spp. And Fasciola spp. The most predominant parasite encountered was A. lumbricoides 46(12.3%) whereas both Toxocara spp. 12(6.9) and Fasciola spp. 2(0.5) were the least detected parasites. It is worth-mentioned that the rate of contamination in Bore market (38.15%) was higher compared with Jiren market (34.7%) and Hora Gibe market (27%). However, the rate of contamination in vegetables and fruit obtained from the three district was non-significant (p = 0.19). Contamination was more common in vegetables than fruits (AOR = 5.78, p<0.001). It was also observed that decreased parasitic contamination was significantly associated with washing the products before displaying it for selling (p < 0.001). The study has identified a high rate of raw vegetables and fruits contaminated with intestinal helminthes and protozoan. Contaminated fresh vegetables and fruits in open-aired peri-urban markets of Jimma city, Ethiopia may play a significant role in transmission of intestinal parasitic infections to humans, particularly A. lumbricoides infection. Therefore, it is urgently needed for health authorities to educate the public on the proper handling of vegetables and fruits prior to consumption.

Distribution characteristics of antibiotic resistance in direct-eating food and analysis of Citrobacter freundii genome and pathogenicity

Purpose This study aims to study the distribution characteristics of antibiotic resistance in direct-eating food and analysis of Citrobacter freundii genome and pathogenicity. Residual antibiotics and antibiotic resistance genes (ARGs) in the environment severely threaten human health and the ecological environment. The diseases caused by foodborne pathogenic bacteria are increasing daily, and the enhancement of antibiotic resistance of pathogenic bacteria poses many difficulties in the treatment of disease. Design/methodology/approach In this study, six fresh fruits and vegetable samples were selected for isolation and identification of culturable bacteria and analysis of antibiotic resistance. The whole genome of Citrobacter freundii isolated from cucumber was sequenced and analyzed by Oxford Nanopore sequencing. Findings The results show that 270 strains of bacteria were identified in 6 samples. From 12 samples of direct food, 2 kinds of probiotics and 10 kinds of opportunistic pathogens were screened. The proportion of Citrobacter freundii screened from cucumber was significantly higher than that from other samples, and it showed resistance to a variety of antibiotics. Whole genome sequencing showed that Citrobacter freundii was composed of a circular chromosome containing signal peptides, transmembrane proteins and transporters that could induce antibiotic efflux, indicating that Citrobacter freundii had strong adaptability to the environment. The detection of genes encoding carbohydrate active enzymes is more beneficial to the growth and reproduction of Citrobacter freundii in crops. A total of 29 kinds of ARGs were detected in Citrobacter freundii, mainly conferring resistance to fluoroquinolones, aminoglycosides, carbapenem, cephalosporins and macrolides. The main mechanisms are the change in antibiotic targets and efflux pumps, the change in cell permeability and the inactivation of antibiotics and the detection of virulence factors and ARGs, further indicating the serious risk to human health. Originality/value The detection of genomic islands and prophages increases the risk of horizontal transfer of virulence factors and ARGs, which spreads the drug resistance of bacteria and pathogenic bacteria more widely.

Sanitary Hygiene and Analysis of Escherichia Coli Bacteria Content in Lalapan Kol at Pecel Lele Trader

The presence of Eschericia coli (E.Coli) bacteria is one indicator of food contamination that can cause food-borne diseases. Fresh vegetables have nutritional advantages because they are consumed raw so that the nutrients contained in them are not denatured or changed. Types of vegetables that are usually consumed fresh have the potential to be detrimental to health because they are susceptible to microbial contamination. This study aims to review the sanitation hygiene of traders and determine the content of E.Coli bacteria in cabbage vegetables from fried catfish traders in one of the sub-districts in the city of Pekanbaru. The type of research used is survey and laboratory analysis. Technique sampling in this study is total sampling technique with a sample of 10 fried catfish traders. The results showed that hygiene and sanitation were still low in all traders. All traders do not use running water for washing vegetables and cutlery, and vegetables are not stored in closed containers. The results of bacterial analysis, it was found that 6 samples of fresh vegetables cabbage fried catfish contained E.Coli and the rest did not contain E.Coli bacteria. It can be concluded that the application of food sanitation hygiene by food handlers at several fried catfish traders in Pekanbaru City is in the poor category, because from 10 samples of cabbage vegetables, E.coli was found in 6 samples of cabbage vegetables.

Polycyclic aromatic hydrocarbons content of food, water and vegetables and associated cancer risk assessment in Southern Nigeria.

The polycyclic aromatic hydrocarbon content of water (four surface water, six underground water (borehole water), seven sachet water), barbecued food and their fresh equivalents (barbecued beef, fish, plantain, pork, yam, chicken, chevon, potato, corn), oil (three palm oil, nine vegetable oil), and fresh vegetable samples (water leaf, bitter leaf, cabbage, carrot, cucumber, pumpkin, garlic, ginger, green leaf, Gnetum Africana, onion, pepper) were determined by GC-MS analysis. The current study also determined the estimated lifetime cancer risk from ingesting polycyclic aromatic hydrocarbon-contaminated food. The polycyclic aromatic hydrocarbon content of water, oil, vegetable, and food samples were within the United States Environmental Protection Agency/World Health Organization safe limits. The naphthalene, benzo(b)fluoranthene, and benzo(k)fluoranthene levels in surface water were significantly higher than in borehole samples (P = 0.000, 0.047, 0.047). Vegetable oils had higher anthracene and chrysene compared to palm oil (P = 0.023 and 0.032). Significant variations were observed in levels of naphthalene, acenaphthylene, phenanthrene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, and dibenzo(a,h)anthracene among the barbecued and fresh food samples (P <0.05). Barbecued pork, potato, and corn had significantly higher naphthalene compared to their fresh equivalents (P = 0.002, 0.017, and <0.001). Consumption of barbecued food and surface water may be associated with higher exposure risk to polycyclic aromatic hydrocarbons which may predispose to increased cancer health risk. The current work explores in depth the concentration of polycyclic aromatic hydrocarbons in different dietary categories that pose direct risk to humans via direct consumption. These findings add knowledge to support future considerations for human health.

A Photonic Label-Free Biosensor to Detect Salmonella spp. in Fresh Vegetables and Meat

This paper presents a method that can be used to detect and identify Salmonella spp. in fresh meat and vegetable samples using a photonic biosensor with specialized bioreceptors. Detection was based on photon transduction. Silicon-nitride-based resonant cavities were used to capture the change in light response when there is specific binding of the immobilized antibody to the sensor surface against the target antigen. A control immobilization experiment was conducted to validate the immobilization process on the biosensor surface prior to biofunctionalization for Salmonella spp. detection. This experiment involved immobilization of pre-selected antibodies on silicon nitride surfaces. Two types of antibodies were suitable. The first was a specific polyclonal antibody with superior antigen-binding capacity across a wide range of concentrations. The second was a monoclonal antibody designed for effective binding at lower concentrations. Rigorous validation was performed. The outcomes were compared with those of the habitual method used to detect Salmonella spp. (reference method). Replicates from different batches of contaminated meat and vegetable samples were analyzed. This comprehensive approach provides a methodologically robust, highly sensitive, and accurate way of rapidly detecting Salmonella spp. in food samples. It has potential implications for improved food safety and quality control.

Effects of different drying methods on the micronutrients of four leafy vegetables traditionally consumed by some Clans in Izzi and Unwana, Ebonyi State, Nigeria

This study was carried out to evaluate the effects of different drying methods (Freeze drying, shade drying and oven drying at varied temperatures of 40oC, 50oC and 60oC) on the micronutrients (mineral and vitamin) contents of L. cupanioides, F. ottonifolia, P. osun and C. pentandra. The minerals contained in both dried and fresh leafy vegetable samples were analyzed by the dry ash extraction method, while vitamins (A, B1, B2, B6 and E) contents of the leafy vegetable samples were determined spectrophotometrically. Vitamin C was determined titrimetrically after homogenizing each sample in 50ml 5.6M EDTA solution. The results from the study revealed that the various drying methods affected the leafy vegetable samples within and between them in the same pattern but to different degrees resulting to moisture lose with increase in minerals and increase in vitamin concentrations when compared with the fresh leafy vegetable samples. Freeze drying and shade drying had the best micronutrients retention effects across all the leafy vegetable samples while oven drying at varied temperatures of 40oC, 50oC and 60oC had gradual decrease in minerals and vitamins retention effects as the temperature increases. The difference in mineral and vitamin concentrations retention with respect to drying methods decreased in this order: Freeze drying &gt; shade drying &gt; oven drying at 40oC &gt; oven drying at 50oC &gt; oven drying at 60oC. The results of the various micronutrients for both fresh and dried leafy vegetable samples in this study ranged as thus: Calcium (72.59–398.10mg/100g), magnesium (41.16–461.07mg/100g), phosphorus (79.28–306.11mg/100g), iron (2.11–32.11mg/100g), zinc (0.19–18.88mg/100g), sodium (29.10–326.51mg/100g), potassium (0.8-196.31mg/100g), vitamin B1 (0.31– 4.22mg/100g), vitamin B2 (0.31 –1.49mg/100g),vitaminB6 (0.36–3.91mg/100g), vitamin A (110.01–548.22ug/g), vitamin C (159.84–818.46mg/100g) and vitamin E (0.16– 9.91mg/100g). In conclusion, the freeze drying and shade drying are the best drying methods for producing dried traditional leafy vegetables with higher micronutrients retention than oven drying at varied temperatures (40oC, 50oC and 60oC).

Evaluation of fresh vegetable microbiological contamination in fast-food restaurants

PurposeFresh vegetables contain advantageous phytochemical components, making them one of the most significant sources of nutrition. The threat of harmful bacteria still exists because these vegetables are not heated in restaurants before being consumed. Therefore, this study aimed to evaluate the microbial quality of fresh vegetables in restaurants of different levels.Design/methodology/approachA total of 499 fresh vegetable samples (from sandwiches and fresh-cut vegetable salads) were collected from 3 different types of food service establishment: 201 from international restaurants (IRs), 210 from national restaurants (NRs), and 88 from cafeterias (CAs). The samples were prepared and inoculated on specific growth media. The aerobic mesophilic bacteria (AMB) Campylobacter spp., Staphylococcus aureus (S. aureus), Enterobacteriaceae, Escherichia coli (E. coli) and yeast and molds were counted, and Listeria monocytogenes, Salmonella spp. and Escherichia coli O157 were detected using specialized medium.FindingsHigh counts of S. aureus, above 3 log cfu/g, suggested that 71.5% of samples collected from NRs and 77.3% from CA were not accepted, whereas 81.6% of samples collected from IRs were accepted. The low population of E. coli, less than 2 log cfu/g, suggested that 99.0, 97 and 92.0 % of samples collected from IRs, NRs and CA, respectively, were accepted. Listeria monocytogenes and Escherichia coli O157 were absent from every sample. One sample was positive for Salmonella spp. in each of the NR and CA sample groups.Originality/valueRIs adhere to health and hygiene standards better than NRs and CAs, according to the findings of vegetable contamination tests.

Detection and Prevalence of Soil Transmitted Helminths Contaminating Several Vegetables in Several Traditional Markets in Surabaya

A total of 100 fresh vegetable samples consisting of yardlong bean, cabbage, lettuce, and lemon basil were purchased from five traditional markets in Surabaya. Each sample was washed with tap water, water washing examined using flotation method to find soil-transmitted helminths. The overall prevalence of soil-transmitted helminths contamination was 41% (41/100). The most predominant parasite was hookworms (20%), followed by Strongyloides stercoralis larvae (14%) and Ascaris lumbricoides eggs (7%). The highest prevalence of contamination was found in lettuce, with a prevalence rate of 56% (14/25), while the lowest prevalence of contamination was found in yardlong bean, with a prevalence rate of 24% (6/25). Sequentially, the prevalence of soil-transmitted helminths contamination of fresh vegetable samples from Pabean traditional market (55%), Keputran traditional market (50%), Wonokromo traditional market (50%), Lakarsantri traditional market (35%) and Mulyosari traditional market (15%), respectively. The present study demonstrated that consumption of vegetables with parasite contamination in this area represents a potential route for the transmission of soil-transmitted helminths infection.

Prevalence, virulence gene profile and antibiogram of Campylobacter jejuni from fresh vegetables in Mymensingh, Bangladesh

This study aimed to investigate Campylobacter jejuni, a major cause of food-borne bacterial infections worldwide, in fresh vegetables from five upzillas (Mymensingh, Trishal, Bhaluka, Muktagacha, and Fulbaria) in the Mymensingh district between July 2020 and April 2023. Using cultural, biochemical, and molecular techniques, 100 fresh vegetable samples (including tomato, carrot, cucumber, green chili, and coriander) were examined for C. jejuni. The isolates were further tested for virulence genes and antimicrobial susceptibility. Out of the 100 samples, 23% were confirmed as C. jejuni, by 16S rRNA gene-based polymerase chain reaction and all were found to be virulent with cytolethal distending toxins (cdtA, cdtB and cdtC genes). Antibiotic susceptibility testing revealed resistance to amoxicillin (47.83%), tetracycline (43.48%), and streptomycin (39.13%) among the isolates. However, ceftriaxone and ciprofloxacin were effective against 47.83% and 43.48% of the isolates, respectively. Moreover, 52.17% of the isolates were sensitive to erythromycin. Alarmingly, 34.78% of the C. jejuni isolates exhibited multidrug resistance (MDR) with eight different antibiotic resistance patterns, including four MDR patterns. These findings highlight the presence of virulent and antibiotic-resistant C. jejuni in fresh vegetables, emphasizing the need for monitoring and control to ensure food safety and public health issues. Asian Australas. J. Food Saf. Secur. 2023, 7 (2), 62-72

Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables

Natural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to identify target regions for the design of species-specific primers. Assay specificity was measured with 12 strains of closely related Burkholderia bacteria and demonstrated the primer pair BCF6/R6 were 100% specific for detection of B. cenocepacia. The described qPCR assay evaluated B. cenocepacia with a 2 pg μl−1 limit of detection and appropriate linearity (R2 = 0.999). In 50 samples of experimentally infected produce (lettuce, onion, and celery), the assay could detect B. cenocepacia as low as 2.6 × 102 cells in each sample equal to 1 g. The established qPCR method quantitatively detects B. cenocepacia with high sensitivity and specificity, making it a promising technique for B. cenocepacia detection and epidemiological research on B. cepacia complex organisms from fresh vegetables.

PARASITIC CONTAMINATION OF VEGETABLES IN SELECTED LOCAL MARKETS IN ADEN GOVERNORATE, YEMEN

Fresh and raw vegetables may play a significant role in transmission of intestinal parasitic infections to humans. Therefore, this study was conducted to estimate the level of parasitic contamination in vegetables which are consumed raw in Aden governorate, Yemen. This is a descriptive analytic cross-sectional study in which six local markets were randomly selected. A total of 216 fresh vegetable samples including parsley (Petroselinum sativum), watercress (Nasturtium officinale), lettuce (Lactuca sativa), leek (Allium porrum), green onion (Allium cepa), and tomatoes (Solanum Lycopersicum) were purchased from the selected markets and analysed using direct and iodine wet mount smear preparation. Modified Ziehl–Neelsen staining and Zinc sulphate flotation method were also used. Smears were examined by under the light microscope. Out of 216 vegetable samples, 166 (76.9%) were contaminated with one or more parasites. Parsley was the most contaminated (97.2%), followed by watercress (94.4%), lettuce (88.9%), leek (80.6%), green onion (77.8%), whereas the least contamination rate was in tomato (22.2%). Statistically significant difference in parasitic contamination rate was found between different types of vegetables (P &lt; 0.05). The most common parasite was Ascaris lumbricoides 75 (24.4%), followed by cyst of Entamoeba histolytica/dispar 64 (20.9%), and Entamoeba coli 52 (16.8%). Significant difference was reported in prevalence rate between different parasitic species (????&lt; 0.05). Vegetable samples collected from Dar Saad market, show the highest contamination rate (94.4%) while vegetables collected from Al Mansura market showed the lowest contamination rate(50.0%). However, the parasitic contamination rates were not significantly different for samples collected from different markets. This study highlighted the importance of raw vegetables as the potential source of transmission for intestinal parasites to humans. Prevention methods such as proper washing or cooking of vegetables before consumption should be conveyed to consumers. In addition, comprehensive health education and hygienic practices, including washing hands after handling vegetables, should be provided to sellers and consumers.

Determination of Parasitic Contamination in Vegetables Collected from Local Markets in İzmir Province, Türkiye.

Fresh vegetables are an important part of a healthy and nutrient-rich diet but the consumption of raw vegetables without proper washing is the main way for transmission of parasites. This study was aimed at determining the rate of parasitic contamination in prewashed fresh vegetables sold at randomly selected 10 retail markets which is the last step to reach the consumer in İzmir, Türkiye. A total of 80 samples selected from eight types of vegetables including tomato, spinach, lettuce, rocket, mint, parsley, dill, and cucumber were examined for parasitic agents microscopically by sedimentation method after washing samples with normal saline. Statistical analysis was performed using SPSS software version 20.0. Protozoan cysts, helminth eggs, and larvae were detected in 21 (26.2%) of 80 samples from eight different vegetable species. Rhabditidiform larvae 18.7%, Blastocystis spp. 5%, Toxocara spp. 2.5%; Ascaris spp., Fasciola spp., Entamoeba histolytica/ Entamoeba dispar and hooked worms were found in 1.2%. Spinach and mint samples were contaminated with parasites significantly more than other fresh vegetable samples (p<0.008, odds ratio =80.0; p<0.017, odds ratio =46.6 respectively). Cruznema spp., a plant nematode, was found at the highest rate according to the results of culture, polymerase chain reaction and sequencing, respectively. In this study, the parasitic contamination was found in approximately one of the four vegetables sold in randomly selected markets in İzmir. These findings show that vegetables sold in local markets can cause parasitic infections if they are consumed without adequate washing and awareness should be raised on this issue. In addition, it was concluded that morphological examinations should be confirmed by molecular studies and sequencing as much as possible in order to avoid misdiagnosis of rhabditidiform larvae.

Prevalence of helminths in fresh vegetables: a narrative literature review.

The consumption of fresh vegetables is related to healthy lifestyle habits present in culinary preparations in different regions. The presence of pathogenic parasites in these foods can cause gastrointestinal disorders. Thus, the objective of the present study was to carry out a narrative review of the literature on the prevalence of helminths in fresh vegetable samples. The analysis of the studies published from 2016 to 2022 showed that hookworms and Ascaris lumbricoides are the most common pathogenic helminths in fresh vegetable samples, with a prevalence of up to 73.8% and 55.1%, respectively. In addition, studies have shown associations between the presence of helminths and pathogenic protozoa. The results obtained in this review indicate the urgent need to implement actions at all stages of the vegetable production chain, from the water used in planting irrigation to cleaning before sale to the final consumer. © 2022 Society of Chemical Industry.

Occurrence of Extended Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram-Negative Bacteria in Fresh Vegetables, an Increasing Human Health Concern in Algeria.

The aim of this study was to screen for extended spectrum cephalosporin-, carbapenem- and colistin-resistant Gram-negative bacteria in fresh vegetables in Batna, Algeria. A total of 400 samples of fresh vegetables were collected from different retail stores. Samples were immediately subjected to selective isolation, then the representative colonies were identified using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF–MS). Phenotypic and genotypic analyses were carried out in terms of species identification and relative antibiotic resistance. Transferability of the carbapenemase and mcr-bearing plasmids was verified by conjugation. The clonal relationships of carbapenemase and mcr-positive Escherichia coli isolates were studied by multi-locus sequence typing (MLST). Sixty-seven isolates were characterised and were mostly isolated from green leafy vegetables, where the dominant species identified included Citrobacter freundii, Klebsiella pneumoniae, Enterobacter cloacae, Stenotrophomona maltophilia, E. coli and Citrobacter braakii. PCR and sequencing results showed that E. coli was the bacterial species presenting the highest antibiotic resistance level in parallel to blaTEM (n = 16) and blaCTX-M-15 (n = 11), which were the most detected genes. Moreover, five isolates carried carbapenemase genes, including the blaOXA-48 and/or blaVIM-4 genes. The mcr-1 gene was detected in two E. coli isolates. MLST analysis revealed three different E. coli sequence types: ST101 (n = 1), ST216 (n = 1) and ST2298 (n = 1). Conjugation assays confirmed the transferability of the blaOXA-48 and mcr-1 genes. In this study we report, for the first time, the detection of the blaOXA-48 gene in E. coli and C. braakii isolates and the blaVIM-4 gene in vegetables. To the best of our knowledge, this is the first report on the detection of mcr-1 genes from vegetables in Algeria.

Quantitative determination of the content OF FE, NI, CR, CU, ZN, CD, PB, HG in vegetables and fruits growed in Moldova and assessment of the health risk

Abstract: In samples of fresh vegetables and fruits, food products of domestic production, selected in rural markets, in shops, in preschool institutions of four pilot districts of Moldova, was determined the content of the following heavy metals: copper, zinc, iron, manganese, molybdenum, nickel, lead, cadmium and chrome. Based on the results of a survey of the population of four pilot rural districts, a list of studied products (fresh vegetables and fruits, food of plant origin) was determined. The results of the analysis showed that the investigated heavy metals accumulated in all samples of fresh vegetables, legumes and in products of processing plant raw materials, with the exception of residual amounts of mercury, the content of which was below the detection limit of the method. Taking into account the daily diet of a rural dweller, it can be assumed that the consumption of foodstuffs containing residues of copper, cadmium and zinc exceeding the MPC may have a negative impact on health.

The contamination rate of raw vegetable with pathogenic protozoa in Sulaimani city, Kurdistan-Iraq

This study was carried out to determine the contamination rate of raw vegetables with the intestinal parasite in Sulaimani province, Kurdistan, Iraq. A total of 300 fresh leafy vegetable samples, including (Dill, green onion, leek, lettuce, garden cress, watercress, peppermint, celery, radish and spinach) were collected from three central markets in the Sulaimani province. Each sample was separately washed with distilled water and/or phosphate buffer saline (PBS). The washing fluid was analyzed to detect parasite using standardized direct and indirect (sedimentation) microscopic examination technique. The total contamination rate of vegetable with the intestinal parasites was 36% (108/300). Giardia lamblia was the most prevalent parasite (9.3%) followed by Entamoeba coli (5%), Trichomonas spp. (4.6%), and Entamoeba histolytica (3.6%). The most contaminated vegetable was Peppermint 70% (21/30), followed by celery 46.6% (14/30), radish and garden cress were having the same contamination rates 43.3% (13/30) and green onion 36.6% (11/30). This finding concludes the raw vegetables in Sulaimani markets are a potential sources of intestinal parasite transmission, and the contamination rate of vegetables was very high and at critical level.