Sample Of Algerian Population Research Articles

Association between CX3CR1rs3732378 polymorphism and neovascular age-related macular degeneration in a sample of Algerian population.

Neovascular age-related macular degeneration (nAMD) is a progressive ocular disease, responsible for central visual loss and blindness in elderly population. Increase data demonstrate that genetic factors play an important role in pathogenesis process of this disease. The aim of this study is to investigate the association between rs3732378 polymorphism in CX3CR1 gene and nAMD in a sample of Algerian patients. This case-control study consisted of 72 patients with nAMD and 124 control subjects. DNA of participants was extracted using salting out method. Genotyping was carried out using the TaqMan real-time polymerase chain reaction method. Statistical analysis was performed by SPSS.21.0. The prevalence of the risk genotype AA was higher in the nAMD group than in control group (OR=5.02, 95% CI=1.44-17.4, P=0.011). In our sample of Algerian patients, the rs3732378 polymorphism is associated with nAMD. This result may support the role of CX3CR1 gene in the pathogenesis of nAMD.

Analysis of vascular endothelial growth factor (VEGF) insertion/deletion gene polymorphism and environmental risk factors in sample of Algerian population with neovascular age-related macular degeneration (nAMD)

Increasing evidence shows that genetic and environmental factors can influence neovascular age-related macular degeneration (nAMD) risk. The aim of this study was first to analyse the association of insertion/deletion polymorphism in VEGF gene and environmental factors with the risk of nAMD, and then to investigate whether these factors have an impact on the age of onset of nAMD in a sample of the Algerian population. Seventy two patients with nAMD and one hundred twenty-four controls were recruited; standardized questionnaire was used to collect information regarding underlying systemic diseases and important environmental factors. Genotyping of VEGF (I/D) SNP was conducted using PCR-based assay approach, and statistical analyses were conducted using IBM SPSS statistics 21. A significant association was reported of age (p < 0.05), smoking (p = 0.02), alcohol (p < 0.01), hypertension (p = 0.04), hyperlipidaemia (p = 0.008) and thyroid disease (p = 0.03) with nAMD. Also, Thyroid disease may have a role in accelerating the development of nAMD in an earlier age in our sample (p < 0.001). No association was found between the VEGF – 2549 I/D genotype and the presence of nAMD (p = 0.27), neither with the age of onset of nAMD (p = 0.21). Our results suggest that age, smoking, alcohol, hypertension, hyperlipidaemia and thyroid diseases are possible risk factors that could increase the risk of nAMD in a sample of Algerian population. In addition, VEGF – 2549 I/D might not be associated with the risk of nAMD development. Finally, thyroid disease may accelerate the development of nAMD in an earlier age.

Association study of Angiotensin-Converting Enzyme Insertion/Deletion gene polymorphism with Neovascular Age-Related Macular Degeneration in a sample of Algerian population

Association study of Angiotensin-Converting Enzyme Insertion/Deletion gene polymorphism with Neovascular Age-Related Macular Degeneration in a sample of Algerian population

Pre-diabetes and NAFLD; A study of an Algerian population sample

IntroductionInsulin resistance is one of the most important mechanisms involved in the genesis of NAFLD (Non-Alcoholic Fatty Liver Disease), which explains the potential risk of diabetes in this population. The objective of our study was to compare the prevalence of pre-diabetes in people with metabolic steatopathy or NAFLD with that of a general population free from hepatic steatosis. Patients and methodsIt is a prospective case-control study which focused on non-diabetic patients aged between 30 and 70 years. A clinical and biological assessment was carried out for all patients. Hepatic steatosis was diagnosed by ultrasound. Insulinemia was measured by Fluorescence Enzyme Immunoassay (Tosoh AIA 360) expressed in µU /Ml. The insulin resistance calculation was done using the HOMA formula. Pre diabetes is defined by the American Diabetes Association (ADA) as a fasting blood sugar (8 hours) between 1 and 1.25 g / l. Statistical analyzes were performed using SPSS 21.0 software (IBM). Results426 patients aged 48.52 ± 10.13 years (226 women (53.6%)), including 213 with fatty liver disease, were enrolled. The mean fasting blood glucose in NAFLD patients was significantly higher (0.97 ± 0.13 213 Vs 0.92 ± 0.11, p <0.001). The prevalence of pre-diabetes was higher in the NAFLDs than in the control population (14.6% vs 8.9%, p <0.001), women were more affected than men (20.4% vs 8%, P <0.01). We noted a positive correlation between pre-diabetes and insulin resistance (n = 426, r = 0.124 P = 0.013). In multivariate analysis, the factors independently associated with pre-diabetes were metabolic syndrome, plasma cholesterol≥ 2g/l, and insulin resistance. ConclusionIn view of these results, we recommend systematic research for pre-diabetes in NAFLDs for effective prevention of diabetes and its complications. Its higher prevalence in this population is probably underpinned by insulin resistance.

Association study of APOE gene polymorphisms with diabetes and the main cardiometabolic risk factors, in the Algerian population

BackgroundMetabolic syndrome (MetS) represents a combination of at least three primary metabolic abnormalities among which obesity, hyperglycemia, dyslipidemia, and high blood pressure (HBP); once combined, they increase the cardiovascular risk significantly. APOE gene is considered as a genetic risk factor for cardiovascular diseases, and it has been linked to MetS or related disorders in several populations. Our study aimed to analyze, for the first time, the association of three APOE gene polymorphisms with MetS risk and its components in a general Algerian population sample, and to highlight the potential influence of these polymorphisms on individual susceptibility to MetS, diabetes, high blood pressure, and obesity, which has never been studied before in the Algerian population.ResultsThe rs439401 showed a significant association with hypertension. The T allele confers a high risk of hypertension with an odds ratio (OR) of 1.46 (95% CI [1.12–1.9], p = 0.006). The rs4420638 polymorphism was significantly associated with obesity in the general population. The G allele provides protection against obesity; the resulting OR is 0.48 (95% CI [0.29–0.81], p = 0.004).ConclusionsAlthough APOE variants were not associated with the risk of MetS, the APOE polymorphism alleles were associated with some of the metabolic parameters in Algerian subjects. The relation of APOE rs439401 alleles with high blood pressure seems indicative of a state of stress of the population.

The relationship between MTHFR C677T gene polymorphism and essential hypertension in a sample of an Algerian population of the Oran city

Background Many studies have investigated the role of 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in essential hypertension (EH), but with conflicting results. Aim To determine the eventual association between 5,10-methylenetetrahydrofolate reductase (MTHFR ) C677T gene polymorphism and hypertension in a sample of Algerian population from the Oran city. Methods A case-control study has been performed in 154 subjects including 82 hypertensives defined as subjects with elevated systolic blood pressure SBD140mmHg and or sustained diastolic blood pressure DBP≥90mmHg, and 72 normotensive subjects. Polymerase chain reaction (PCR) combined with restrictive fragment length polymorphism (RFLP) was used to detect the MTHFR C677T variant. Results We observe no significant differences between allelic and genotypic frequencies between cases and controls for C677T polymorphism (OR=1.51, 95% CI= 0.89-2.56, P=0.13). Analyses adjusted for age, sex and body mass index improved the association level, though the association was still not significant (30% vs. 22%, OR=1.75, 95% CI= 0.95-3.24, P=0.07). &nbsp; &nbsp; Conclusion This work showed that genetic polymorphism related to the MTHFR gene (C677T) is not associated with the risk of hypertension in this sample of Algerian population. Larger case- control samples are required to clearly assess the role of this genetic variant in EH. Keywords: MTHFR C677T gene polymorphism; hypertension; Algerian population; case-control study.

The relationship between MTHFR C677T gene polymorphism and essential hypertension in a sample of an Algerian population of Oran city

BackgroundMany studies have investigated the role of 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in essential hypertension (EH), but with conflicting results. AimTo determine the eventual association between 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and hypertension in a sample of Algerian population from the Oran city. MethodsA case–control study has been performed in 154 subjects including 82 hypertensives defined as subjects with elevated systolic blood pressure SBD≥140mmHg and or sustained diastolic blood pressure DBP≥90mmHg, and 72 normotensive subjects. Polymerase chain reaction (PCR) combined with restrictive fragment length polymorphism (RFLP) was used to detect the MTHFR C677T variant. ResultsWe observe no significant differences between allelic and genotypic frequencies between cases and controls for C677T polymorphism (OR=1.51, 95% CI=0.89–2.56, P=0.13). Analyses adjusted for age, sex and body mass index improved the association level, though the association was still not significant (30% vs. 22%, OR=1.75, 95% CI=0.95–3.24, P=0.07). ConclusionThis work showed that genetic polymorphism related to the MTHFR gene (C677T) is not associated with the risk of hypertension in this sample of Algerian population. Larger case–control samples are required to clearly assess the role of this genetic variant in EH.

Association study between some renin-angiotensin system gene variants and essential hypertension in a sample of Algerian population: case control study.

Essential hypertension is an important risk factor for the development of cardiovascular disease. We aim in this study to analyse the relationship between AGT M235T gene variant and ACE I/D gene variant with essential hypertension in a sample of the Algerian population of the Oran city. A case-control study has been performed in 145 subjects including; 75 hypertensives and 70 controls from Algerian population of Oran city. Polymerase chain reaction (PCR) combined with restrictive fragment length polymorphism (RFLP) was used to detect the M235T variant of angiotensinogen (AGT) gene and a nested PCR to determine ACE I/D gene variant. The genotypic and allelic distribution of the M235Tvariant of the AGT gene did not differ in hypertensives and normotensives group (X(2) =7.81, P<0.05; X(2) =4.67, respectively) thus there was no association between the T allele and hypertension (OR=1.64; 95%CI [1.01-2.69]). The genotypic and allelic frequencies of the ACE I/D variant did differ significantly between hypertensives and controls (X(2)=13.98, P<0.05; X(2) =12.66, P<0.05, respectively) where a significant association between the D allele of the ACE I/D gene and essential hypertension has been observed (OR=0.46; 95%CI [0.27-0.75]). We reported a high prevalence of the D and T allele in hypertensives female. This study shows that the M235T variant of the AGT gene is not associated with essential hypertension while a significant association has been reported with the D allele in this sample of Algerian population of the Oran city.

Combined effect of established BMI loci on obesity-related traits in an Algerian population sample.

BackgroundGenome-wide association studies have identified variants associated with BMI in populations of European descent. We sought to establish whether genetic variants that are robustly associated with BMI could modulate anthropometric traits and the obesity risk in an Algerian population sample, the ISOR study.The ISOR study of 787 adult subjects (aged between 30 and 64) provided a representative sample of the population living in the city of Oran (north-west of Algeria). We investigated the combined effect of 29 BMI established genetic variants using a genetic predisposition score (GPS) on anthropometric traits and obesity risk in 740 subjects.ResultsWe found that each additional risk allele in the GPS was associated with an increment in the mean [95% CI] for BMI of 0.15 [0.06 - 0.24] kg/m2 (p = 0.001). Although the GPS was also associated with higher waist (p = 0.02) and hip (p = 0.02) circumferences, these associations were in fact driven by BMI. The GPS was also associated with an 11% higher risk of obesity (OR [95%CI] = 1.11 [1.05 - 1.18], p = 0.0004).ConclusionsOur data showed that a GPS comprising 29 BMI established loci developed from Europeans seems to be a valid score in a North African population. Our findings contribute to a better understanding of the genetic susceptibility to obesity in Algeria.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-014-0128-1) contains supplementary material, which is available to authorized users.

Effects of established blood pressure loci on blood pressure values and hypertension risk in an Algerian population sample.

Genome-wide association studies and subsequent replication studies have pinpointed 29 genetic variants associated with blood pressure (BP). None of these studies included North African populations. We therefore looked at whether or not these genetic variants modulated BP and hypertension (HTN) risk in an Algerian population sample. Twenty-nine single-nucleotide polymorphisms (SNPs) were genotyped in a representative sample of 787 subjects from the InSulino-résistance à ORan (ISOR) study (378 men and 409 women aged between 30 and 64 years and recruited from within the city of Oran, Algeria). Genetic variants were considered both individually and when combined as genetic predisposition scores (GPSs) for systolic BP (SBP), diastolic BP (DBP) and HTN risk. The SNPs in CYP1A1-ULK3, HFE and SH2B3 were significantly associated with BP and/or HTN. The SBP-GPS, DBP-GPS and HTN-GPS were associated with higher levels of DBP (+0.24 mm Hg P=0.05, +0.23 mm Hg P = 0.05 and +0.26 mm Hg P = 0.03, respectively). Moreover, the three GPSs tended to be associated with a 6% higher risk of HTN. Our study is the first to show that some of the BP loci validated in subjects of European descent were associated (either individually or when combined as GPSs) with BP traits and/or the HTN risk in an Algerian population, but to a lesser extent than in European populations. Although larger studies and meta-analyses of North African populations are needed to confirm the present results, our data contribute to a better understanding of genetic susceptibility to HTN.

Detection of CFTR mutations using PCR/ARMS in a sample of Algerian population.

Cystic fibrosis (CF) is the most common autosomal recessive disease in Caucasians. Wrongly considered as a European disease, CF is found in Algeria; but the literature data on the clinical profile and the spectrum of CFTR gene mutations are poor. In this study we investigate twenty-four unrelated Algerian families, with at least one child with CF. DNA extracts from blood samples of patients and parents were screened for CFTR gene mutations using Elucigene CF30 Kit which is based on a PCR/ARMS technique. Only five different mutations were identified. On the 48 alleles studied, most common mutations were: c.1521_1523delCTT (F508del) 18.75%, c.579+1G>T (711+1G>T) 12.5%, c.1624G>T (G542X) 10.41%, c.3909C>G (N1303K) 4%, and c.1652G>A (G551D) 2%. The Elucigene CF30 kit highlights a portion of CFTR mutations in the Algerian population. It remains important for a first screening as it reveals the most common mutations. All this information is of interest for genetic testing and genetic counseling in Algeria and in European countries where immigration from the Maghreb is common.

Relationship between Angiotensinogen gene T174M Polymorphism and Essential Hypertension in a Sample of Algerian Population: Case Control Study

Relationship between Angiotensinogen gene T174M Polymorphism and Essential Hypertension in a Sample of Algerian Population: Case Control Study

Analysis of 21 X-chromosomal STRs in an Algerian population sample

Twenty-one X-chromosomal short tandem repeat loci, including the six clusters of linked markers DXS10148-DXS10135-DXS8378 (Xp22), DXS7132-DXS10074-DXS10079 (Xq12), DXS6801-DXS6809-DXS6789 (Xq21), DXS7424-DXS101 (Xq22), DXS10103-HPRTB-DXS10101 (Xq26), DXS8377-DXS10146-DXS10134-DXS7423-DXS10011 (Xq28), and the loci DXS6800 and GATA172D05 were typed in a northwestern Algerian population sample (n = 210; 104 men and 106 women). Allele and haplotype frequencies were calculated. No evidence of linkage disequilibrium was observed between pairs of loci within clusters of linked markers. At locus DXS10148, sequence analysis of a subset of alleles displaying unusual amplicon length (>/= 36 repeat units) and anomalous electrophoretic mobility showed that this marker has a complex molecular structure with different repeat variants within alleles of identical amplicon size.

Analysis of 12 X-chromosomal STRs in an Algerian population sample

Twelve X-chromosomal short tandem repeat (STR) loci (DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, DXS8377, DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, DXS10011), including two clusters of closely linked markers (DXS6801–DXS6809–DXS6789 mapping in Xq21 and DXS7424–DXS101 mapping in Xq22) were typed in a northwestern Algerian population sample ( n = 210; 104 men and 106 women). The calculated allele and haplotype frequencies were compared with those previously obtained for the same set of markers in the Italian population. No evidence of linkage disequilibrium was observed between pairs of loci within clusters of strictly linked markers.

Évaluation primaire des troubles du comportement alimentaire chez le sujet obèse et le patient diabétique de type 2 algérien

Glucose imbalance in type 2 diabetes and massive weight gain in obese subjects are disorders that are correlated with Behaviour Eating Disorder (BED), leading to a hypercaloric, sweet and fat oriented diet. This study examined how BED such as dietary restriction, disinhibition, and hunger, are assessed by Three Factor Eating Questionnaire (TFEQ) and Dutch Eating Behaviour Questionnaire (DEBQ). In the present investigation these factors were studied in a sample of Algerian population composed of 30 obese subjects, 70 type 2 diabetic patients and 30 healthy subjects. 51 questions or items were used for the TFEQ test and 33 questions for the DEBQ test. Multivariate analysis showed that restriction and disinhibition were significantly associated with obesity and diabetes pathogenesis. Compared to healthy subjects, obese patients showed a significant hyperphagic BED type (disinhibition and hunger disorders). These BED are associated with psycho-sensory disorders as characterized by externality and emotivity. Conversely, the diabetic patient showed both hypophagic and hyperphagic BED. In this study, the role of BED in obesity genesis and in glycaemia dysregulation of the diabetes pathology seems to be confirmed. An early identification of these disorders deserves further investigation in a broad Algerian population in order to treat them.

Analysis of Y-chromosomal SNP haplogroups and STR haplotypes in an Algerian population sample

The distribution of Y-chromosomal single nucleotide polymorphism (SNP) haplogroups and short tandem repeat (STR) haplotypes was determined in a sample of 102 unrelated men of Arab origin from northwestern Algeria (Oran area). A total of nine different haplogroups were identified by a panel of 22 binary markers. The most common haplogroups observed in the Algerian population were E3b2 (45.1%) and J1 (22.5%). Y-STR typing by a 17-loci multiplex system allowed 93 haplotypes to be defined (88 were unique). Striking differences in the allele distribution and gene diversity of Y-STR markers between haplogroups could be found. In particular, intermediate alleles at locus DYS458 specifically characterized the haplotypes of individuals carrying haplogroup J1. All the intermediate alleles shared a common repeat sequence structure, supporting the hypothesis that the variant originated from a single mutational event.