Sugar-salt Solution Research Articles

Эффективность регидратирующей терапии при вирусных гастроэнтеритах у детей

Aim: to compare the efficacy of reduced osmolarity oral rehydration solution (ORS) with zinc and infusion therapy with salt and sugar-salt solutions in children suffering from viral gastroenteritis (VGE) complicated by dehydration syndrome of I-II stage and II stage. Patients and Methods: a prospective open-ended comparative randomized clinical trial was conducted The children were divided into two groups: the main group (n=45) with therapy used reduced osmolarity ORS (carbohydrates 2.84 g, glucose 2.82 g, chlorides 0.453 g, zink 0.003 g, osmolarity: 245 mOsm/L, per 1 sachet), and the comparison group (n=34), receiving standard infusion therapy with salt-sugar solutions. The severity of acute gastroenteritis was assessed using a modified Vesikari score. Comprehensive clinical and laboratory examination of children was performed before and after treatment Results: the mean severity of the patients' condition on the modified Vesikari score was 13.26±0.7 points for VGE, which corresponds to the moderate severity. In 42 (93.3±4.5%) patients of the main group, the condition stabilization and reduction of pathological losses were achieved during the first 6 hours, and therefore oral rehydration was continued. In this case, the active use of ORS was conducted for 24–48 hours with an assessment of the trend in general and topical symptoms, as well as laboratory parameters of the fluid and electrolyte balance and renal excretion of nitrogenous compounds. 3 patients (6.66±3.7%) who required further parenteral fluid administration were excluded from the study due to the lack of proper oral rehydration effect associated with the vomiting and the impossibility of further therapeutic manipulations. During oral rehydration in the main group, the febrile reaction duration was comparable in the lasting with the comparison group. Normalization of general well-being in the form of the fatigue disappearance was noted in the compared groups at the same time, while the vomiting cessation was recorded in a significantly shorter time in the main group. Exicosis and diarrhea were managed to be stopped in the main and comparison groups at almost comparable time. Conclusion: rehydration with low-osmolar ORS helps to avoid possible complications of parenteral fluid administration and a traumatic situation for a child KEYWORDS: exicosis, dehydration syndrome, viral gastroenteritis, acute intestinal infection, oral rehydration, oral rehydration therapy, oral rehydration solution, zinc, low-osmolar oral rehydration solution, children. FOR CITATION: Bekhtereva M.K., Komarova A.M. Rehydration therapy efficacy of viral gastroenteritis in children. Russian Journal of Woman and Child Health. 2024;7(2):177–183 (in Russ.). DOI: 10.32364/2618-8430-2024-7-2-14.

AMBA CUP: Ensuring Accuracy in Measurement of Volume of Water for Salt Sugar Solution or Oral Rehydration Solution Preparation in Diarrhea Management.

Diarrhea remains a major cause of under-five mortality globally. In 2016, it accounted for 8% of under-five mortality worldwide. Most of these deaths occur in developing countries. Fluid replacement using Oral Rehydration Solution (ORS) or Salt Sugar Solution (SSS), has been the mainstay of diarrhea management. Gaps in knowledge and practice regarding the preparation of these solutions have been identified by various researchers. One challenge encountered by healthcare providers and caregivers of under-five children has been lack of a standard, easy to clean cup for measurement of accurate volume of water for ORS or SSS preparation. Soft drink bottles, which are currently being used, are difficult to clean because of their narrow necks. More so, the size and volume of these bottles change so often that non-numerate caregivers get easily confused. The aim of this paper is to introduce the AMBA CUP, an easy-to-clean cup that can be used to accurately measure one litre of water for SSS or ORS preparation.

Determining the Efficacy of Albendazole Against STH Infection Among Orang Asli

Introduction: According to WHO, soil-transmitted helminth (STH) infections could infect up to 90% of children from deprived communities with unhygiene and improper sanitation lifestyle. In West Malaysia, commonly the aboriginal people (Orang Asli) is prone to be infected with STH infections. Albendazole (400mg) is an anthelminthic drug that has been used for more than 30 years to cure STH infections. Hence, the main objective of this project was to evaluate the efficacy of single-dose albendazole (400mg) against STH infections among Orang Asli. Methods: Stool samples were collected in Hulu Langat, Semenyih, Malaysia. Who had not previously undergone deworming treatment. The parasites were concentrated by floatation technique (using the standard salt-sugar solution) to extract the amount of helminths’ egg before and after the albendazole treatment (400mg). Results: There were 44 participants after one-month post-treatment. The cure rate (CR) for Trichuris trichiura and Ascaris lumbricoides was 11.6% (n=5) and 35.0% (n=14), respectively. Moreover, there was no heavy infection for both STHs during the post-treatment of albendazole. Conclusion: The results indicated that the chosen anthelminthic showed higher cure rate efficacy in A.lumbricoides than T.trichiura. However, both species had decreased egg burden post-treatment with albendazole.

Factors Influencing the Use of Oral Rehydration Solution among Mothers of Under Five Children Attending Holy Rosary Specialist Hospital and Maternity Waterside In Onitsha North Lga, Anambra State.

Purpose: This study determined factors influencing the use of oral rehydration solution among mothers with children below five years of age attending Holy Rosary Specialist Hospital and Maternity Waterside in Onitsha North LGA, Anambra State.
 Methodology: A cross-sectional descriptive design was adopted in conducting the study among mothers with children under five years attending Rosary Specialist Hospital and Maternity, Waterside in Onitsha North Local Government Area, Anambra State. A sample size of one hundred and fifty (150) women was selected by convenience sampling techniques. A self-structured questionnaire with a reliability coefficient of 0.98 was used in gathering the necessary data suitable for the study which were analysed with SPSS version 20.0.
 Findings: It was discovered that majority of the women 141(94.6%) have heard of ORS and they have adequate understanding of the solution, the two different types of oral rehydration solutions (that is solution from oral rehydration salt and salt sugar solution) but do not use SSS hence lack adequate knowledge of its preparation. Also, the mothers 116(95.9%) used ORS when their children had diarrhoea while the remaining 5(4.1%) did not use ORS because their children never had diarrhoea. 65(43.6%) of the women have all the materials needed for the preparation of SSS while 70(47.0%) which were the majority did not know the materials required for the preparation of ORS hence they could not indicate if they had the materials or not. From the hypothesis tested, there is a significant relationship between mothers’ level of education (p=0.030), mother’s occupation (p=0.024), number of under five children (p=0.045) and the use of ORS. From the study, it was ascertained that socio-demographic factors like mother’s level of education, occupation and number of under five children affected the use of ORS in the population used. Mother’s level of knowledge of the solution, availability and accessibility of the ORS sachet (environmental factor) and the occurrence of diarrhoea in a child (Child factor) also affect use of ORS.
 Recommendations: Health workers in the health care facilities should intensify their effort in the provision of adequate health education to the mothers (may be to be incorporated into antenatal care health talks) on the preparation of Salt Sugar Solution (SSS) type which is cost effective and readily available. Also the hospital management should ensure that Oral Rehydration salt sachets are available in the hospitals and at an affordable price so as to ensure authenticity of the product used by the women.

THE LEVEL OF MOTHER’S KNOWLEDGE ON THE USE OF ZINC IN TODDLERS WITH DIARRHEA IN KARANG PRING HAMLET, RW 01, SUMBER JERUK VILLAGE

Diarrhea is influenced by several factors, including environmental conditions, community behavior, community services, nutrition, population, education which includes knowledge, and socio-economic conditions. Diarrhea is defecation in the form of liquid more than three times in one day and lasts for two or more days. The cause of diarrhea is mostly due to infection.Prevention of diarrhea that can be done is to continue breastfeeding, formula milk, and solid baby food, give ORS or sugar-salt solution to replace lost fluids, give foods that contain fiber, and give zinc for 10 consecutive days.The purpose of this study was to determine the level of mother's knowledge of the use of zinc in toddlers with diarrhea in Karang Pring RW 01, Sumber Jeruk village in the realm of knowing, understanding, and application. The method used is descriptive method with cross sectional analysis research design. In this study, the researcher used a validated questionnaire instrument. The sampling technique used total sampling and obtained 44 samples.Based on the results of data analysis, it was obtained that the percentage level of mother's knowledge on the use of zinc in toddlers with diarrhea in Karang Pring RW 01, Sumber Jeruk village for the July 2021 period in the tofu domain was 64.6%, the understanding domain was 74.2%, and in the application domain 72.7 %. The conclusion in this study is that the realm of knowing is categorized as sufficient, the realm of understanding is categorized as sufficient and in the realm of application it is categorized as sufficient. Keywords: Knowledge level, Diarrhea, zinc

THE LEVEL OF MOTHER’S KNOWLEDGE ON THE USE OF ZINC IN TODDLERS WITH DIARRHEA IN KARANG PRING HAMLET, RW 01, SUMBER JERUK VILLAGE

Diarrhea is influenced by several factors, including environmental conditions, community behavior, community services, nutrition, population, education which includes knowledge, and socio-economic conditions. Diarrhea is defecation in the form of liquid more than three times in one day and lasts for two or more days. The cause of diarrhea is mostly due to infection.Prevention of diarrhea that can be done is to continue breastfeeding, formula milk, and solid baby food, give ORS or sugar-salt solution to replace lost fluids, give foods that contain fiber, and give zinc for 10 consecutive days.The purpose of this study was to determine the level of mother's knowledge of the use of zinc in toddlers with diarrhea in Karang Pring RW 01, Sumber Jeruk village in the realm of knowing, understanding, and application. The method used is descriptive method with cross sectional analysis research design. In this study, the researcher used a validated questionnaire instrument. The sampling technique used total sampling and obtained 44 samples.Based on the results of data analysis, it was obtained that the percentage level of mother's knowledge on the use of zinc in toddlers with diarrhea in Karang Pring RW 01, Sumber Jeruk village for the July 2021 period in the tofu domain was 64.6%, the understanding domain was 74.2%, and in the application domain 72.7 %. The conclusion in this study is that the realm of knowing is categorized as sufficient, the realm of understanding is categorized as sufficient and in the realm of application it is categorized as sufficient.Keywords: Knowledge level, Diarrhea, zinc

A Descriptive Cross-Sectional Assessment of Caregivers' Knowledge and Practices Regarding the Prevention and Management of Diarrhea among Children under the Age of Five in Thulamela B Clinics, South Africa.

Diarrhea is a common cause of child-related hospitalization and mortality among children under the age of five in South Africa. This study was conducted to assess the knowledge and practices of caregivers regarding prevention and management of diarrhea among children under the age of five in the Thulamela Municipality of South Africa. A quantitative approach using a descriptive cross-sectional survey was used. A questionnaire was adopted to collect data from caregivers at thirty primary health-care facilities using convenient sampling. Statistical Package for Social Sciences (SPSS) version 24.0 was used to analyze data. Most of the respondents have fair knowledge about diarrhea, oral rehydration therapy/salt sugar solution and its usage during diarrheal episodes. However, most of them (81.7%) do not use the salt sugar solution when their children have diarrhea. Almost all the respondents practice a hand washing hygiene for themselves and their children; 97.2% do not reheat cooked food before feeding their children; 95.5% do not drink untreated water as their source of drinking water is the municipal supply. The practices of these respondents do not reflect their knowledge in terms of the use of oral rehydration solution/salt and sugar solution. Further efforts should be made to educate caregivers on the mode of transmission of diarrheal pathogens.

Oligosaccharide and antinutrient content of whole red haricot bean fermented in salt–sugar and salt‐only solutions

AbstractCommon beans (Phaseolus vulgaris L.) are nutritious and confer numerous health benefits. However, they are also high raffinose family oligosaccharides (RFOs) and antinutrients. Appreciable amounts of RFOs and antinutrients remain after soaking and cooking, causing flatulence and lowered mineral bioavailability to bean consumers. Fermentation has been shown to lower RFOs and antinutrients in bean flours and milk. However, beans are majorly consumed as whole grains. The purpose of this study was to develop a protocol for fermenting whole common beans. We fermented boiled whole red haricot beans and evaluated their effect on RFOs, tannins, and phytates. A factorial research design was used. Beans were sorted, soaked for 15 h, and boiled for 1 h. The beans were then fermented in 2% salt–sugar solution (SSF) and 2% salt‐only solution (SOF) for 120 h. Microbial growth and pH were monitored every 24 h during fermentation. After fermentation, the beans were dried, milled, and the flours subjected to biochemical analysis. Fermentation favored the growth of lactic acid bacteria (LAB), lowering the pH to 3.88 and 5.26 in SSF and SOF batches, respectively. Tannin content reduced significantly by 64.70% and 73.19% in the SSF and SOF batches, respectively. Phytates reduced by 58.88% and 68.85%, respectively. Raffinose reduced significantly by 96.40% and 95.01%, respectively, whereas stachyose reduced by 95.92% and 94.11%, respectively. The highest reduction of antinutrients and RFOs occurred between 24 and 72 h of fermentation. Higher antinutrient losses occurred in the SOF batch, whereas higher RFO losses occurred in the SSF batch.

Effect of Lb. plantarum BFE 5092 Fermentation on Antinutrient and Oligosaccharide Composition of Whole Red Haricot Bean (Phaseolus vulgaris L).

Common beans are a leguminous plant of the genus Phaseolus. They are rich in protein, energy, and minerals. They confer a wide range of health benefits when consumed. Utilization of common bean has however been poor due to high antinutrient content that results in reduced nutrient digestibility and mineral bioavailability. Flatulence after consumption is also a huge deterrent to common bean consumption. Lactic acid fermentation is the most common form of food fermentation with the Lactobacilli spp dominating most spontaneous fermentations. The objective of this study was to determine the effect of lactic acid bacteria (LAB) on the antinutrient and flatulence causing oligosaccharide composition of red haricot bean. A factorial research design was used in the study. Red haricot beans were sorted and soaked for 15 h. The soaked beans were fermented in 2% salt-sugar solutions for 120 h. Experimental batch was inoculated with Lb. plantarum BFE 5092 (IF), and the control batch was spontaneously fermented (SF). Microbial growth and pH were monitored every 24 h during fermentation. After fermentation, the beans were dried and milled, and the flours were subjected to biochemical analysis. ANOVA was done using SPSS statistics 23. The pH decreased significantly (P < 0.05) from 6.06 to 3.9 in both batches at the end of fermentation. The LAB counts significantly increased (P < 0.05) in both batches, whereas coliform counts decreased significantly (P < 0.05). Fungi were not detected in both batches. Soaking lowered tannins and phytates and raffinose concentrations significantly but had no significant effect on stachyose concentration. At the end of 120 h of fermentation, the tannin content was 109.50 and 54.04 mg/100 g in IF and SF, respectively. Phytates were at 242.52 and 163.43 mg/100 g in IF and SF, respectively. Raffinose content was 32.85 and 32.58 mg/100 g in IF and SF, respectively, while stachyose content was 593.33 and 467.49 mg/100 g in IF and SF, respectively. This research showed that LAB is able to ferment soaked whole red haricot and lower the tannin, phytate, raffinose, and stachyose content significantly. Spontaneous fermentation lowered these antinutrients and oligosaccharides better than inoculation with Lb. plantarum BFE 5092.

Optimisation of osmotic dehydration process for aonla (Emblica officinalis) fruit in the mixture of salt-sugar solution

The osmotic dehydration of aonla fruit was carried out to optimize the time of the osmosis in the mixed solution of sugar and salt at the room temperature. The destined aonla fruits with and without blanching were immersed in the mixture of salt and sugar solution. The salt concentration of the osmotic solution was kept constant (100 g) and the sugar concentrations were varied to bring the concentrations to 35, 45 and 55o Brix maintaining a fruit to syrup ratio of 1:3 by weight. The observations were recorded to study the influence of osmotic solution and time on water loss and solid gain. The results of osmotic dehydration showed the maximum water loss of 48.55 per cent recorded for blanched fruit osmosed at 55o B and the minimum (27.61%) in unblanched fruit osmosed at 35oB. The solid gain also was observed to be maximum (23.4%) in the blanched fruit compared to unblanched fruit (9.94%) at respective concentrations. The optimization process carried out using Design expert software showed the highest desirability of 0.785 for blanched aonla fruit osmosed at 55 oB.

Sensory and Microbiological Properties of Pickled Bamboo Shoots (Bambusa vulgaris)

Most people currently preserve bamboo shoots (Bambusa vulgaris) that have been harvested using alum which has an adverse effect on the human body. Alternative preservation of bamboo shoots can be done by making bamboo shoots using a solution of sugar and salt solution. This study aims to determine the effect of salt and sugar concentrations on the sensory and microbiological properties of pickled bamboo shoots. The design used was the factorial method in the basis of a completely randomized designs (CRD) using two factors, namely salt solution and sugar solution. The formulation used were sugar concentration 1%; 2%; 3% and salt concentration 10%; 12.5%; 15% Sensory test results showed that the concentration of sugar and salt affected the hedonic test values of color, aroma, and texture. Whereas microbiological analysis showed that there is an increase in the total plate count by increasing fermentation time

Identifying childhood diarrhoea training needs of Nigerian frontline health care workers

Background: Health education and case management are important in the reduction of morbidity and mortality resulting from childhood diarrhoea. Objective: To identify the training needs of health care workers as a measure of their empowerment to fulfil their roles in the control of childhood diarrhoea. Methods: This cross-sectional, descriptive study utilized a self-administered pre-tested questionnaire to obtain demographic information, knowledge about diarrhoea, features of dehydration, management, and prevention of diarrhoea from 165 health care workers drawn from the 18 local government areas of Edo state. Results: Only 24 (14.5%) of health care workers could correctly define diarrhoea. Majority of respondents could identify features of dehydration: thirst 161 (97%), loss of skin turgor 149 (90.3%) and irritability 142 (86.1%) but 93 (56.4%) and 52 (31.5%) did not know that unconsciousness and passage of small volume/no urine respectively were features of dehydration. Only 15 (9.1%) could correctly constitute Salt Sugar Solution (SSS). Up to 42.2% of health workers use antibiotics regularly to manage childhood diarrhoea while another 48.8% considered the use of antibiotics during the diarrhoeal episode a preventive strategy. Conclusion: Frontline health care workers need periodic training and retraining with consistent supportive supervision to ensure that children with diarrhoea are appropriately evaluated and managed.

Osmotic dehydration kinetics of biofortified yellow-flesh cassava in contrast to white-flesh cassava (Manihot esculenta).

In recent times, the cultivation, processing and consumption of biofortified yellow-flesh cassava is of significant interest to breeders and food processors due to its relatively high pro-vitamin-A content, compared to the conventional white-flesh cassava. In light of this, osmotic dehydration (OD) kinetics of a recently released biofortified yellow-flesh cassava was compared to that of a white-flesh cassava, using salt, sugar, and salt-sugar solutions at different temperatures (30, 45, 60°C) and fixed cube/solution-ratio. Water loss (WL) and solids gain (SG) data were fitted by non-linear regression using four models (Page, Weibull, Azuara, and Peleg). Azuara model was most appropriate in describing OD kinetics for both cultivars. Azuara estimates for equilibrium WL and equilibrium SG, respectively, ranged between 0.101-0.120 and 0.049-0.094g/g for salt solution, 0.158-0.212g/g and 0.107-0.268g/g for sugar solution and 0.234-0.306g/g and 0.189-0.276g/g for salt-sugar solution. The best conditions for OD of both cultivars by salt solution and sugar solution was at 60°C and 45°C, respectively, while that for salt-sugar solution varied with cultivar. Increasing temperature increased water loss and solids gain. Salt-OD conformed to Arrhenius temperature dependence of diffusivity, but sugar-OD and salt-sugar-OD did not. Micrographs reveal biofortified yellow-flesh cassava was more susceptible to cell wall collapse than white-flesh cassava. Extent of dehydration by OD agents ranked: salt-sugar > sugar > salt. Osmotic dehydration may be useful as a means of dehydration for cassava prior to drying, and is especially relevant for the carotenoids-rich biofortified yellow cassava.

Assessment of knowledge of mothers from migrant population regarding use of oral rehydration solution in diarrheal illness in under-five children from rural Pune

Background: Diarrhea is one of the important causes of morbidity and mortality among under-five children in India. Lack of knowledge of mothers about the management of diarrheal diseases is a common problem in many developing countries. Use of oral rehydration solution (ORS) largely depends, on the level of mother’s knowledge. Objective: The objective of the study was to assess the knowledge of the mothers, from rural migrant population regarding the use of ORS in under-five children with diarrhea. Materials and Methods: A cross-sectional study was carried out from December 2016 to March 2017, among mothers of the migrant population residing and working at brick kilns and construction site in the field practice area of the rural health training center, Lavale, Pune. We included the mothers whose children were under five years of age. Those mothers, who had serious illness and had hearing and speech problems, were excluded from the study. Data were collected by interview method from mothers with the help of a pre-structured and pre-tested questionnaire. Results: A total of 54 (93.1%) mothers from brick kiln sites were included among whom, 43.10% were aware of ORS. Only 37.93% of mothers knew the use of homemade sugar salt solution for diarrhea in children. In this study, 25 (43.10%) mothers were illiterate and the study showed that knowledge regarding ORS was lower in illiterate mothers. Conclusion: Majority of the mothers were not aware regarding ORS, and as per the study, knowledge regarding ORS was lower in less educated mothers. Government schemes would help these mothers to increase the awareness which will result in a decrease in mortality rate.

Mechanical and osmotic dehydration behavior of pineapple and retention of Vitamin C

The study was designed to observe the behavior of mechanical and osmotic dehydration of pineapple, retention of vitamin C content during drying and development of jam from fresh and dehydrated pineapple. Osmotic dehydration carried out with pineapples collected from local market of Mymensingh in July 2015 and found that pineapples contained higher moisture content (93.9%,wb) than normal (85-87%,wb) and showed contradictory osmotic behavior due to higher moisture content and hormonal effect. Then the studies were conducted using pineapples of Gaint Kew variety collected from Madhupur without hormone treatment which contained 86.11% moisture, 0.36% ash, 13.89% total solid, 0.54% protein and 17.38mg/100g vitamin C. The osmotic concentration behavior was investigated using sugar and combined sugar-salt solution for 6mm thick pineapple slices and immersion time was 6 hrs. The extent of water loss, solid gain and normalized solid content were strongly influenced by strength of osmotic solution. It was found that K-value (mass transfer coefficient) increases with increasing concentration and was the highest (0.15min-1) for 55/5% sugar/salt solution and lowest (0.07min-1) for 45% sugar solution. Three different temperatures (55, 60 and 65°C) and thicknesses (4, 6 and 8mm) were used to investigate the drying behavior of pineapple slices in a mechanical dryer. Activation energy value of 8.14 Kcal/g-mole was found for fresh pineapple slices. 55/5% sugar/salt osmosed and dried pineapple gave 5.46 times higher dryer throughput compared to nonosmosed dried pineapple. Degradation of vitamin C content of pineapple at different air-dry bulb temperatures (55, 60 and 65°C) of 6mm thickness was investigated and activation energy for degradation of vitamin C was found to be 14.38 Kcal/g-mole. Osmotic dehydration prior to air drying, gave the lower rate of degradation of vitamin C during drying compared to that dried without osmosis. Developed jams were tested for their acceptability by sensory evaluation using 1-9 point hedonic scale and jam made from osmotically dehydrated (55/5% sugar-salt and then dried at 60°C) secured highest score (8.5) and ranked as “like very much”, while the other products were ranked as “like moderately”.

بررسی اثرتعداد لایهها و غلظتهای مختف پوششهای خوراکی کربوکسیمتیلسلولز و کاراگینان بر کارایی فرایند خشک کردن اسمزی سیب زرد لبنانی

در طی فرایند آبگیری اسمز، مواد جامد محلول توسط بافت ماده غذایی جذب شده و اثرات نامطلوبی بر خروج آب، خصوصیات تغذیه‌ای و حسی می‌گذارد. لذا برای بهبود فرایند اسمز از پوشش‌های خوراکی استفاده می‌شود. بهترین شاخص برای ارزیابی یک پوشش، کارایی فرایند (نسبت خروج آب به جذب مواد جامد) است. در این پژوهش از دو پوشش کربوکسی‌متیل‌سلولز و کاراگینان در سه غلظت 5/0، 1 و 5/1 درصد، به‌صورت یک‌لایه و دو لایه، جهت پوشش‌دهی قطعات مکعبی سیب زرد لبنانی و محلول اسمزی ساکارز با غلظت 30، 45 و 60 % استفاده شد. نتایج نشان داد که نمونه یک بار پوشش داده شده با کاراگینان 1% و تیمار شده در ساکارز 60 % و نمونه‌های یک و دو لایه پوشش داده شده با کربوکسی‌متیل‌سلولز 5/0% بیشترین میزان کارایی فرایند را داشته‌اند و به‌عنوان تیمارهای برگزیده به‌دست آمدند. این نمونه‌ها میزان جذب مواد جامد را کاهش داده و تاثیر قابل توجهی بر روی خروج آب نگذاشته‌اند. همچنین نتایج بیان‌گر این موضوع است که با افزایش غلظت محلول اسمزی ساکارز از 30 به 60% میزان حذف آب و جذب مواد جامد افزایش می‌یابد.

Analysis on the securityof preoperative oral sugar salt solution ofpatients with laparoscopic gallbladder excision

Objective To evaluate the effect of the preoperative oral sugar salt solution on patients with laparoscopic gallbladder excision. Methods A total of 178 patients with laparoscopic gallbladder excision were divided into observation group and control group with 89 cases in each. The control group adopted routine fasting way before operation. The patients in the observation group were treated with 300 ml of the sugar salt solution at the 10 o'clock on the evening before surgery. After midnight, nothing by mouth was allowed, except a single morning dose of 200 ml of the corresponding solution.The morning drink was taken at 2h before surgery. Visual analog scales(VAS) were used to score 11 different preoperative discomfort variables.Operational clinical and surgical data were recorded, which included the blood pressure, heart rate, blood glucose levels. Results The control group at different time measured blood glucose, heart rate, systolic pressure and diastolic pressure were statistically different (F=5.609, 5.771, 15.780, 12.335, P 0.05). And blood glucose after 24h, the heart rate after 6h, blood pressure after 6h time compared with other time points statistically significant differences(P 0.05). On the morning before surgery, patients in the control group mainly unwell feeling nervous, hunger, thirst, fatigue, pain, VAS score were significantly increased than those in the observation group(P<0.05). Conclusions Drinking the sugar salt solution at the 10 o'clock on the evening before surgery and 2h before surgery does not affect anesthesia intubation.and not increase the risk of regurgitation and aspiration during or after surgery and alleviatenervous, hunger, thirst, fatigue, pain. Key words: Sugar salt-solution; Fasting for solids and liquid; Celioscope operation; Laparoscopic cholecystectomy; Perioperative period

Evaluation of Mass Transfer Kinetics for Osmotic Dehydration of Oyster Mushrooms (Pleurotus sajor-caju) in Salt-sugar Solution

Oyster mushrooms (Pleurotus sajor-caju) were osmotically dehydrated in a salt-sugar solution to remove moisture prior to hybrid microwave drying. Sodium chloride-sugar concentration (15 %:45°B–15 %:65°B), solution temperature (30–60 °C) immersion time (15–240 min) and solution to fruit ratio (4:1 to 8:1) were taken as independent process variables. The effect of various process parameters on mass transfer kinetics was studied. An analytical solution for unsteady-state mass transfer based on Fick’s second law of diffusion was carried out to mathematically describe water loss and solute gain. Effective water diffusivity was in the range of 3.0–1.59 × 10−9 m2/s whereas solute diffusivity values were between 9.44 × 10−10 m2/s and 1.02 × 10−9 m2/s, under these experimental conditions. Based on statistical results, page model gave best goodness of fit on the experimental data.

Genetically distinct isolates of Spirocerca sp. from a naturally infected red fox (Vulpes vulpes) from Denmark

Spirocerca lupi causes formation of nodules that may transform into sarcoma in the walls of aorta, esophagus and stomach of infected canids. In February 2013, post mortem examination of a red fox (Vulpes vulpes) hunted in Denmark revealed the presence of several nodules containing adult worms of Spirocerca sp. in the stomach and the omentum. The nodules largely consisted of fibrous tissue with infiltration of mononuclear cells, neutrophilic granulocytes and macrophages with hemosiderin deposition. Parasitological examination by three copromicroscopic methods, sedimentation, flotation with saturated sugar-salt solution, and sieving failed to detect eggs of Spirocerca sp. in feces collected from the colon. This is the first report of spirocercosis in Denmark, and may have been caused by a recent introduction by migrating paratenic or definitive host. Analysis of two overlapping partial sequences of the cox1 gene, from individual worms, revealed distinct genetic variation (7–9%) between the Danish worms and isolates of S. lupi from Europe, Asia and Africa. This was confirmed by phylogenetic analysis that clearly separated the Danish worms from other isolates of S. lupi. The distinct genetic differences of the current worms compared to other isolates of S. lupi may suggest the presence of a cryptic species within Spirocerca.

Mass exchange evaluation during optimization of osmotic dehydration for Oyster mushrooms (Pleurotus sajor-caju) in salt-sugar solution

The objective of this study was to investigate the osmotic dehydration of Oyster mushrooms in salt-sugar solution at different solution concentrations, immersion times, temperatures and solution to fruit ratio to analyze the water loss, solute gain and weight reduction. Salt-sugar uptake and water transfer were quantitatively investigated during osmotic dehydration of Oyster mushrooms using response surface methodology. Experiments were conducted in a thermostatically controlled agitating incubator. With respect to water loss, solute gain and weight reduction both linear and quadratic effects of four process variables were found to be significant. For each response, second order polynomial models were developed using multiple linear regression analysis. ANOVA was performed to check the adequacy and accuracy of the fitted models. The response surfaces and contour maps showing the interaction of process variables were constructed. Applying desirability function method, the optimum operating conditions were found to be: solution temperature – 42.3° C, immersion time – 44.21 min, salt-sugar concentration – 15 %: 52.57° B and solution to fruit ratio 4.99:1. At these optimum values, water loss, solute gain and weight reduction was 41, 2.15 and 38.6 (g/100 g initial mass) respectively.