Salt Content Of Foods Research Articles

Umami and saltiness enhancements of vegetable soup by enzyme-produced glutamic acid and branched-chain amino acids.

One major challenge of reducing salt content in food is the risk of the overall taste becoming bland. Enhancing saltiness is an effective strategy for salt reduction, and the development of salt-reduced foods using these saltiness-enhancing flavorants as food additives is underway. However, an increasing number of consumers demand a reduction in additives in clean-label foods. Enzyme processing of food is an attractive strategy for developing clean-label foods because enzymes are not considered additives. We aimed to improve the saltiness and umami intensity of vegetable soups by enzyme treatment while meeting clean-label requirements. We first optimized the enzymatic reaction conditions of a protease and glutaminase blend and then investigated the synergistic effects of this enzyme blend on the taste of vegetable soup. Sensory evaluations indicated that the reaction products (e.g., protein hydrolysates or amino acids) could enhance the umami, kokumi, and saltiness intensity of vegetable soup supplemented with 0.5% NaCl. Notably, the saltiness intensity ratio of the enzyme-treated soup with 0.50, 0.45, and 0.40% NaCl were increased by 1.31-, 1.16-, and 0.99-fold, respectively, when this ratio for the control soup with 0.50% NaCl was set to 1.0. This indicates a 20% salt reduction rate can be achieved by enzyme treatment. Moreover, we found that these enhancements were synergically caused by enzyme-produced glutamic acid and branched-chain amino acids. Our findings suggest that using enzyme blends of bacterial and fungal proteases and glutaminase is an effective approach to enhancing the saltiness levels of vegetable soups while meeting clean-label requirements.

The Jamaica Salt Consumption Study Protocol: Sodium Intake; Sodium Content in Restaurant Foods; Knowledge, Attitudes, and Practices; Spot Urine Sodium Validation.

Excess dietary salt consumption is a major contributor to hypertension and cardiovascular disease. Public education programs on the dangers of high salt intake, and population level interventions to reduce the salt content in foods are possible strategies to address this problem. In Jamaica, there are limited data on the levels of salt consumption and the population's knowledge and practices with regards to salt consumption. This study therefore aims to obtain baseline data on salt consumption, salt content in foods sold in restaurants, and evaluate knowledge, attitudes, and practices of Jamaicans regarding salt consumption. The study is divided into four components. Component 1 will be a secondary analysis of data on urinary sodium from spot urine samples collected as part of a national survey, the Jamaica Health and Lifestyle Survey 2016-2017. Component 2 will be a survey of chain and non-chain restaurants in Jamaica, to estimate the sodium content of foods sold in restaurants. Component 3 is another national survey, this time on a sample 1,200 individuals to obtain data on knowledge, attitudes and practices regarding salt consumption and estimation of urinary sodium excretion. Component 4 is a validation study to assess the level of agreement between spot urine sodium estimates and 24-hour urinary sodium from 120 individuals from Component 3. This study will provide important baseline data on salt consumption in Jamaica and will fulfil the first components of the World Health Organization SHAKE Technical Package for Salt Reduction. The findings will serve as a guide to Jamaica's Ministry of Health and Wellness in the development of a national salt reduction program. Findings will also inform interventions to promote individual and population level sodium reduction strategies as the country seeks to achieve the national target of a 30% reduction in salt consumption by 2025.

5.A. Workshop: Reducing and monitoring sugar, fat, and salt in packaged food: Germany and the European context

Abstract Today, packaged food constitutes more than 50 % of the food supply in most European households. Many of those products are high in energy, sugar, fat, and salt, and consuming them is positively associated with overweight and obesity. Measures such as taxation and reformulation of packaged food high in sugar, fat, or salt are considered effective means to improve food environments and facilitate healthier food choices for individuals. However, the measures as well as their implementation and evaluation vary across Europe. The aim of this workshop is to give an overview of the different actions taken to stimulate the reformulation of food and beverages. Furthermore, we will present the German monitoring of packaged food, including selected findings, as one example to guide such reformulation initiatives. In the first part of our workshop we summarize existing measures in Europe to facilitate health-promoting food choices, focusing on reformulation measures and related monitoring approaches: Presentation 1 will give a systematic overview of measures to reduce sugar, fat, and salt content of food and beverages in selected European countries (Abstract 1). With presentation 2, we will give methodological insights into the German monitoring of the energy and nutrient content in packaged food (Abstract 2). Presentation 3 will focus on the latest results of the German product monitoring, with an emphasis on sugar reduction in soft drinks, yoghurts, and breakfast cereals. Furthermore, we will compare our results to those of other European countries and highlight methodical differences (Abstract 3). In the second half of the workshop, we aim to discuss our findings with the participants. Participants are encouraged to contribute further examples and experiences from their countries, which then will be discussed and documented. Opening questions and polls will be used to stimulate active involvement. Our workshop aims to contribute to better reporting of existing measures and reformulation monitoring methods in Europe, as information is often limited and only available in respective national languages. Moreover, we hope to create a community of practice to continue exchange on the effectiveness of the respective measures and good practice models across Europe. Key messages • The workshop gives an overview of different measures to reduce sugar, fat, and salt content in packaged food in Europe, incl. related monitoring. • The workshop provides a platform to exchange experiences in packaged food monitoring and stimulate scientific exchange and transparency within this area.

Measures to reduce sugar, fat, and salt content of food and beverages in selected European countries

Abstract Introduction Improving food environments to promote healthy diets is a core element of many nutrition and health policies. Government-driven attempts at achieving such improvements comprise mandatory and voluntary measures to reduce the content of sugar, fat, and salt in food and beverages. Here we provide an overview of the different measures taken in selected European countries. Methods A systematic internet search for ongoing or planned national reduction measures in each of the three categories of taxation, legal limits/bans, and self-commitments was conducted for 19 European countries. Core characteristics of each measure, including evaluations thereof, were extracted and summarized. Results The measures varied in, e.g., the nutrients, product groups, and sectors addressed (manufacturing, retail, out-of-home), the timeframe, and the target values. Taxation (15 countries) mainly concerned sweetened beverages, with tax levels mostly depending on the sugar content. Independent evaluations concluded that the tax introduction had led to the sugar content being reduced as well as consumer demand shifted towards lower-sugar options. Six countries had legal limits for the salt content in bread (limits between 1.3 g and 2 g/100 g bread), and nine countries set legal limits for sugar, fat, and salt in food or meals or vending bans for products high in these nutrients in food service for the public sector. The limited evaluations of compliance with the upper limits were ambivalent. Voluntary commitments (12 countries) mainly concerned the reduction of salt and sugar. Independent studies and monitoring results on the achievement of the agreements showed mixed results. Conclusions National measures to reduce sugar, fat, and salt in food and drinks varied in content and depth, and thus in their likely ability to contribute to a healthier food environment. More comprehensive monitoring and evaluation is needed to better assess the effectiveness of the different approaches.

Effect of white pepper addition on the sensory perception of sodium‐reduced soup with an emphasis on saltiness perception

AbstractChemical irritants, like piperine in pepper, have been identified to have cross‐modal interactions, including increasing the perception of saltiness. Processed foods have used cross‐modal interactions (e.g., odor and taste) to improve acceptability of salt‐reduced foods. Cross‐modal interactions between piperine and taste could be applied to increase consumer perception of saltiness in low‐sodium food products. As such the objective of this study was to determine how the addition of white pepper to low‐sodium soup affects its' sensory perception. First, the aroma detection threshold (n = 60) of white pepper was determined. Then white pepper (at detection threshold) was added to a low‐sodium soup and evaluated using hedonic scales, general labeled magnitude scale for saltiness, check‐all‐that‐apply (CATA), and temporal check‐all‐that‐apply (TCATA; n = 76). The results indicated that the saltiness intensity increased with the addition of white pepper based on the results of the general labeled magnitude scale; however, this result was not confirmed using CATA or TCATA. Rather peppery, bitterness, sourness, and strong aftertaste attributes dominated the participants' perception of the soup. The addition of white pepper also decreased the participants' overall liking of the soup as well as their liking of the flavor and texture. Future research should continue to investigate the cross‐modal interactions of white pepper.Practical ApplicationThe food industry is acting in response to consumer concerns by reducing the salt content in their foods. Despite being a positive initiative for human health, a reduction in the salt content of foods can yield a reduction in sensory appeal and flavor intensity. This study investigated the use of white pepper to increase the saltiness perception of low‐sodium soup. The results indicated that white pepper added bitterness and strong aftertaste to the soup while simultaneously suppressing other flavors. This study also found that white pepper decreased the acceptability of the soup and indicated that white pepper may not be an ingredient that can mitigate the reduction in salt in food products.

Cross-Classification Analysis of Food Products Based on Nutritional Quality and Degree of Processing.

This study aims to compare the classification of foods available in the Portuguese market using Nutri-Score and NOVA classifications and to analyse their ability to discriminate the fat, saturated fat, sugar, and salt content of foods. A sample of 2682 food products was collected. The nutritional quality of foods was established using the Nutri-Score, classifying them into five categories (from A to E). The NOVA classification was used to classify foods according to the degree of food processing into unprocessed/minimally processed foods, processed culinary ingredients, processed foods, and ultra-processed foods (UPF). The nutritional content of food products was classified using a Multiple Traffic Light label system. It was observed that 73.7% of UPF were classified as Nutri-Score C, D, and E, 10.1% as Nutri-Score A, and 16.2% as Nutri-Score B. Nutri-Score was positively correlated with NOVA classification (ρ = 0.140, p < 0.001) and with the Multiple Traffic Lights system (ρTotal Fat = 0.572, ρSaturated Fat = 0.668, ρSugar = 0.215, ρSalt = 0.321, p < 0.001). NOVA classification negatively correlated with the Multiple Traffic Lights system for total fat (ρ = -0.064, p < 0.001). Our findings indicate the presence of many UPFs in all Nutri-Score categories. Since food processing and nutritional quality are complementary, both should be considered in labelling.

RESEARCH PROGRESS OF SALT REDUCTION: FROM INITIATIVES TO TECHNOLOGICAL INNOVATIONCTION

Salt is one of the most widely used condiments in the home cooking and the food industry. It not only contains an essential micronutrient for human beings (sodium), but also is an important and safe preservative in food processing sector. Excessive salt intake is linked to the high prevalence of hypertension and other related diseases such as cardiovascular diseases and stroke. A reduction in salt intake across the whole population will be conducive to improvements in public health. However, reducing the salt content in foods is not a simple but a challenging task, since salt not only impacts the flavor and texture of the final products, but also plays an important role in processing and preservation of foods. The present review introduced the salt reduction actions around the world and different approaches for sodium reduction in foods, and further summarized emerging technologies that utilized in salt reduction in foods.

Correlaciones entre la ingesta de sal en la dieta y la eliminación de sodio en la orina de 24 h en una muestra de población urbana de Cáceres

ObjectiveExcessive salt intake is associated with higher levels of AHT and prevalence of cardiovascular diseases. WHO recommends consuming less than 5 g/day of salt (equivalent to 2 g Na+/day). Identifying foods and behaviours with greater contribution to excess intake would facilitate preventive dietary advice. DesignObservational study. SiteUrban Health Center in Cáceres. Main measurementsWe estimated salt consumption using two food consumption frequency (FFQ) questionnaires, one generic and one with high Na+ content, and a 24 h follow-up survey. We use the Evalfinut program for nutritional evaluation of the diet. We analyzed correlations between estimated salt intake and 24-h urine sodium elimination (gold standard). Results92% of the population had consumption higher than the recommendations with intakes equivalent to 9.5 g/day of salt (3.7 g/d of Na+). When sodium intake is determined by the Na+/K+ ratio, 79.54% have high intakes. Sodium elimination follows a slightly increasing trend with BMI. The perception of salt consumption is low, 56.3% consider it “adequate”. 32.4% add salt to food once served. FFQ underestimate Na+ intake and targeted surveys provide higher values. The correlation between FFQ and Na+ urinary elimination is weak. ConclusionWe must reduce salt intake by increasing the perception of consumption, improving knowledge about the salt content in food and identifying target patients of the health council.

Changes in the salt content of packaged foods sold in supermarkets between 2015-2020 in the United Kingdom: A repeated cross-sectional study.

Excess consumption of salt is linked to an increased risk of hypertension and cardiovascular disease. The United Kingdom has had a comprehensive salt reduction programme since 2003, setting a series of progressively lower, product-specific reformulation targets for the food industry, combined with advice to consumers to reduce salt. The aim of this study was to assess the changes in the sales-weighted mean salt content of grocery foods sold through retail between 2015 and 2020 by category and company. Information for products, including salt content (g/100 g), was collected online from retailer websites for 6 consecutive years (2015 to 2020) and was matched with brand-level retail sales data from Euromonitor for 395 brands. The sales-weighted mean salt content and total volume of salt sold were calculated by category and company. The mean salt content of included foods fell by 0.05 g/100 g, from 1.04 g/100 g in 2015 to 0.90 g/100 g in 2020, equivalent to -4.2% (p = 0.13). The categories with the highest salt content in 2020 were savoury snacks (1.6 g/100 g) and cheese (1.6 g/100 g), and the categories that saw the greatest reductions in mean salt content over time were breakfast cereals (-16.0%, p = 0.65); processed beans, potatoes, and vegetables (-10.6%, p = 0.11); and meat, seafood, and alternatives (-9.2%, p = 0.56). The total volume of salt sold fell from 2.41 g per person per day to 2.25 g per person per day, a reduction of 0.16 g or 6.7% (p = 0.54). The majority (63%) of this decrease was attributable to changes in mean salt content, with the remaining 37% accounted for by reductions in sales. Across the top 5 companies in each of 9 categories, the volume of salt sold decreased in 26 and increased in 19 cases. This study is limited by its exclusion of foods purchased out of the home, including at restaurants, cafes, and takeaways. It also does not include salt added at the table, or that naturally occurring in foods, meaning the findings underrepresent the population's total salt intake. The assumption was also made that the products matched with the sales data were entirely representative of the brand, which may not be the case if products are sold exclusively in convenience stores or markets, which are not included in this database. There has been a small decline in the salt content of foods and total volume of salt sold between 2015 and 2020, but observed changes were not statistically significant so could be due to random variations over time. We suggest that mandatory reporting of salt sales by large food companies would increase the transparency of how individual businesses are progressing towards the salt reduction targets.

The Jamaica salt consumption, Knowledge, Attitudes and Practices (Salt-KAP) study: A research protocol

Background: Excess dietary salt consumption is a major contributor to hypertension and cardiovascular disease. Public education programs on the dangers of high salt intake, and population level interventions to reduce the salt content in foods are possible strategies to address this problem. In Jamaica, there are limited data on the levels of salt consumption and the population’s knowledge and practices with regards to salt consumption. This study therefore aims to obtain baseline data on salt consumption, salt content in foods sold in restaurants, and evaluate knowledge, attitudes, and practices of Jamaicans regarding salt consumption. Methods: The study is divided into four components. Component 1 will be a secondary analysis of data on urinary sodium from spot urine samples collected as part of a national survey, the Jamaica Health and Lifestyle Survey 2016-2017. Component 2 will be a survey of chain and non-chain restaurants in Jamaica, to estimate the sodium content of foods sold in restaurants. Component 3 is another national survey, this time on a sample 1,200 individuals to obtain data on knowledge, attitudes and practices regarding salt consumption and estimation of urinary sodium excretion. Component 4 is a validation study to assess the level of agreement between spot urine sodium estimates and 24-hour urinary sodium from 120 individuals from Component 3. Discussion: This study will provide important baseline data on salt consumption in Jamaica and will fulfil the first components of the World Health Organization SHAKE Technical Package for Salt Reduction. The findings will serve as a guide to Jamaica’s Ministry of Health and Wellness in the development of a national salt reduction program. Findings will also inform interventions to promote individual and population level sodium reduction strategies as the country seeks to achieve the national target of a 30% reduction in salt consumption by 2025.

RELATIONSHIP BETWEEN DIET PATTERNS AND HYPERTENSION EVENTS IN POLARA PUBLIC HEALTH CENTER IN KONAWE ISLANDS DISTRICT

Background: Hypertension is a non-communicable disease that is still a health problem in the work area of the Polara Health Center since 2018 until now in 2020. Hypertension has increased in cases in the last three years. In 2018 8 146 cases of hypertension then increased to 154 cases in 2019 and decreased in 2020. The results of the initial survey showed that 70% of hypertensive patients had unhealthy eating patterns such as consuming fast food containing preservatives, too high salt content in food, excess fat consumption. This study aims to determine the relationship between diet and the incidence of hypertension in the Polara Health Center Work Area ,Konawe Islands Regency. Methods: This research uses analytical research method with Cross Sectional Study approach. The population in this study were all outpatients seeking treatment at the Polara Public Health Center for the period January to February 2021 totaling 268 people, with a total sample of 73 people selected by simple random sampling technique. Results: The results showed that 43.8% of respondents who have a poor diet and have hypertension ,9.6% who do not have hypertension. Then there are 13.7% of respondents who have a good diet but have hypertension ,32.9% who do not have hypertension. The results of statistical tests using the chi square test obtained a p-value of 0.000 and a coefficient of Phi (φ) = 0.531, so the null hypothesis was rejected, meaning that there was a moderate relationship between diet and the incidence of hypertension at Polara Health Center, Konawe Islands Regency. Conclusions: diet has significant impact to hypertension at Polara Health Center, Konawe Islands Regency.

The Bacterial and Fungi Microbiota of Soy Sauce-Supplied Lactic Acid Bacteria Treated with High-Pressure Process

Background: Soy sauce is applied as a seasoning in daily life in East Asia. High amounts of salt in soy sauce can inhibit microbial growth in its production and preservation. However, the elevated salt content in food might increase the health risk. Low salt fermentation is rarely used in soil sauce production because of the potential harmful lactic acid bacteria growth. Therefore, dilution after high salt fermentation is commonly used to reduce the salt concentration. Methods: This study aims to treat the low salt fermented soy sauce with a high hydrostatic pressure process (HPP) to eliminate the harmful bacteria and to analyze the microbial community change compared with that in traditional high salt fermentation. Results: The results showed that the bacterial diversity was increased in low-salt and high-pressure (LS-HP)-treated soy sauce, though the bacterial abundance was decreased. Relative abundance in high-salt (HS), low-salt (LS), and LS-HP-treated soy sauce showed specific bacterial strains in the LS-HP group. Similarly, the fungal diversity was also increased in LS-HP-fermented soy sauce and the detected OTUs were increased. The fungi sensitive to salinity and pressure were indicated in our results. Conclusions: The present study suggests the enhanced bacterial and fungal diversity and different microbial community in HS, LS, and LS-HP-treated soy sauce, as well as the availability of LS and HPP treatment on soy sauce production.

Impact of a Shorter Brine Soaking Time on Nutrient Bioaccessibility and Peptide Formation in 30-Months-Ripened Parmigiano Reggiano Cheese.

Reducing the salt content in food is an important nutritional strategy for decreasing the risk of diet-related diseases. This strategy is particularly effective when applied to highly appreciated food having good nutritional characteristics, if it does not impact either upon sensory or nutritional properties of the final product. This work aimed at evaluating if the reduction of salt content by decreasing the brine soaking time modifies fatty acid and protein bioaccessibility and bioactive peptide formation in a 30-month-ripened Parmigiano Reggiano cheese (PRC). Hence, conventional and hyposodic PRC underwent in vitro static gastrointestinal digestion, and fatty acid and protein bioaccessibility were assessed. The release of peptide sequences during digestion was followed by LC–HRMS, and bioactive peptides were identified using a bioinformatic approach. At the end of digestion, fatty acid and protein bioaccessibility were similar in conventional and hyposodic PRC, but most of the bioactive peptides, mainly the ACE-inhibitors, were present in higher concentrations in the low-salt cheese. Considering that the sensory profiles were already evaluated as remarkably similar in conventional and hyposodic PRC, our results confirmed that shortening brine soaking time represents a promising strategy to reduce salt content in PRC.

Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration

Arginyl dipeptides like Arg-Ser, Arg-Ala, and Arg-Gly are salt-taste enhancers and can potentially be used to reduce the salt content of food. The l-amino acid ligase RizA from B. subtilis selectively synthesizes arginyl dipeptides. However, industrial application is prevented by the high cost of the cofactor adenosine triphosphate (ATP). Thus, a coupled reaction system was created consisting of RizA and acetate kinase (AckA) from E. coli providing ATP regeneration from acetyl phosphate. Both enzymes were recombinantly produced in E. coli and purified by affinity chromatography. Biocatalytic reactions were varied and analyzed by RP-HPLC with fluorescence detection. Under optimal conditions the system produced up to 5.9 g/L Arg-Ser corresponding to an ATP efficiency of 23 g Arg-Ser per gram ATP. Using similar conditions with alanine or glycine as second amino acid, 2.6 g/L Arg-Ala or 2.4 g/L Arg Gly were produced. The RizA/AckA system selectively produced substantial amounts of arginyl dipeptides while minimizing the usage of the expensive ATP.

Saltquanti – Development of a new portable device to analyse salt content in food

Excessive salt intake is a growing concern in society, impacting on a number of serious clinical conditions, such as the development of hypertension and increased mortality/morbidity caused by cardiovascular diseases. The urgent need to introduce in situ assessment of the salt content of processed foods led to the development of SALTQUANTI, a portable device developed and patented by a consortium promoted by the company EVOLEO Technologies and the University of Porto, which combines sample preparation with the analytical detection by means of a sodium selective electrode with potentiometric detection. The device includes the milling and stirring process of the sample, together with the analytical measurement and data interpretation via a touch display. Data for the analytical validation of the method were obtained from 20 different processed food samples. The linearity of the method was in the range from 0.06 to 6.00 g salt/100 g food. The precision was of 6% on average, considering repeatability of results for different types of processed food and the accuracy was of 9% on average. Interlaboratorial comparison showed deviation of results of less than 20 % for the different types of samples. The analytical procedure proved to be simple and reliable, and automation enabled the analysis in less than 5 min with an acceptable uncertainty.

Size-dependent enhancement in salt perception: Spraying approaches to reduce sodium content in foods

There is a rising need to come up with scalable approaches for reducing the salt content in foods. The product structure design is one of the mechanisms to reduce sodium content without impacting the taste of food. Here we report a new approach involving modification of the size and structure of common salt using two techniques: spray drying (SD) and electrohydrodynamic atomized drying (ED). We characterized the properties of the SD and ED produced salts; with size ranges of 1.21 μm and 520 nm, 2-fold and a 3-fold increase in saltiness were observed, indicative of around 58.67 and 65.34% reduction in sodium levels respectively, to achieve similar levels of saltiness for control salt. Finally, these findings were supported with time-intensity analysis and dissolution characteristics of these salts, demonstrating a promising approach for reducing sodium content in foods, particularly for surface-salting applications.

Effect of salt and monosodium glutamate on the sensory characteristics of low‐sodium cheese‐flavored corn grits expanded snacks

The effects of salt and monosodium glutamate on the sensory characteristics of cheese-flavored corn grits expanded snacks, looking to reduce the sodium content of these products, were studied. An increase in the percentage of salt increased the ideal of the intensity of salty taste, the degree of liking of salty taste, and the degree of liking of flavor, while an increase in the monosodium glutamate percentage increased only the first two previous sensory responses. No interactions were observed between salt and monosodium glutamate considering results from Response Surface Methodology. From the experimental design, four samples were selected and submitted to the Temporal Dominance of Sensations analysis. In general, the snacks presented umami taste as a dominant attribute. Among the snacks studied, that with 1.2% salt and 0.6% monosodium glutamate stands out, since this was sensorially accepted, presented umami taste as its dominant attribute, and still had estimated sodium content below the average for commercial snacks. Practical applications Nowadays there are efforts for reducing the salt consumption in reason to relations of such ingredient with some non-communicable chronic diseases. However, the reduction of salt content in food is not a simple task because sodium compounds play an important role in the flavor of foods. Therefore, studying how some compounds rich in sodium, such as salt and monosodium glutamate, influence the sensory characteristics of products, in this case, of expanded snacks of cheese-flavored corn grits, is relevant for providing alternatives for reducing the sodium content on products, reduction that must be gradual so as not to negatively affect consumer acceptance.

Innovative equipments to monitor and control salt usage during culinary

Abstract Issue In Portugal, most of the salt consumed comes from the salt added during preparation/cooking. Therefore, it is urgent to introduce mechanisms to allow the quantification of the salt in foods, and the salt added during culinary. Description of the Problem To tackle salt reduction, we considered: having a portable and user-friendly instrument that measure salt content of foods; and controlling the amount of salt added to foods during culinary, assessing its impact on health. The first project was SALT QUANTI (co-funded by NORTE 2020), promoted by a consortium between EVOLEO Technologies and the University of Porto (finished in September 2018, duration 18 months); its major deliverable was to obtain a prototype of a portable device and the analytical procedure that enabled the analysis of salt content in foods/meals produced in catering kitchens, in less than 5 minutes. The second project was iMC SALT (supported by FCT, Grant POCI-01-0145-FEDER-029269), started in July 2018 (duration 36 months), aiming to develop prototypes for controlling the amount of salt added to foods during culinary, at home and catering, according international recommendations; one clinical intervention to assess the impact on health of the prototype at home is underway. Results The SALT QUANTI and iMC SALT prototypes showed good results in validation tests. The preliminary results of the health impact of the iMC SALT prototype will be presented at the conference. Lessons The implementation of innovative equipments to help consumers and catering industry to monitor or control the addition of salt during preparation of food or cooking are expected to be available soon and to be useful to consumers and catering industry to reduce salt intake. Key messages Innovative equipments may contribute to estimate salt in foods, and to reduce salt added during culinary. The implementation of an equipment to control culinary salt could be one valid approach to reduce salt consumption with impact on health.

Angiotensin I-Converting Enzyme (ACE) Inhibitory and Antioxidant Activity of Umami Peptides after In Vitro Gastrointestinal Digestion

Umami peptides can help reduce the salt content in foods while still maintaining a savory taste. Few studies have reported the bioactivity of umami peptides after consumption. We studied the bioactivities of 12 umami peptides after gastrointestinal digestion. Three umami peptides exhibited angiotensin I-converting enzyme (ACE) inhibitory and antioxidant activity after digestion. Six novel peptides were identified from digestion solutions of the peptides by HPLC-MS/MS. Among them, CC, CCNK, and HCHT had both ACE inhibitory activity (IC50 values were 9.81, 9.00, and 114.99 μM, respectively) and antioxidant activity (strong 1,1-Diphenyl-2-pycryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging activities). AHSVRF had strong ACE inhibitory activity. These peptides increased the nitric oxide concentration and decreased the content of endothelin-1 in a medium of human umbilical vein endothelial cells in a dose-dependent manner. Experiments with damaged HepG2 cells showed that peptides CC, CCNK, and HCHT had antioxidant activity through their cytoprotective effects and by reducing the reactive oxygen species content. The results indicated that umami peptides may provide many health benefits after consumption.

The associations between genetics, salt taste perception and salt intake in young adults

Food liking is one of the main determinants of food intake. Salt taste perception and preference, that play a role in liking of salt, may be genetically determined, although research in humans is scarce. The aim of this study was to explore the associations between genetics, salt taste perception, preference, self-reported salt habit and intake. The participants were young (18–35 years) and healthy adults (32 males and 63 females). Salt taste thresholds were determined with British Standard ISO3972:2011 methodology and salt taste preference by ratings of saltiness and pleasantness of tomato soup with salt concentrations reflecting salt content in foods. Self-reported salt habit was determined by asking participants how salty they usually eat their food and salt intake with two 24-hour 5-step multiple pass recalls. Genotyping for variants in the SCNN1B rs239345 and TRPV1 rs8065080 was performed. Participants homozygous for the minor allele of the rs8065080 had lower ratings of saltiness (p = 0.008) and higher ratings of pleasantness of soup (p = 0.027) when compared to major allele carriers. Preference for salt in soup was associated with salt habit (p = 0.003) and participants with high salt preference had higher salt intake compared to those with low salt preference (2236 ± 261 vs. 1543 ± 107 mg/1000 kcal, p = 0.017). TRPV1 rs8065080 may play a role in salt taste perception and preference, which should be confirmed in a larger sample size study. Hedonic appeal of salty food should be considered when providing personalised advice to change this behaviour.