Background: Copper and selenium were proven modulaters of reproductive function in ruminants under dietary supplementation. Their effects under pareneral administraton during estrus synchronization are not clearly defined. On this background the present was aimed to evaluate the effect of copper and selenium on estrus associated responses in goats. Methods: Thirty adult female Salem black goats were selected. On day ten of the estrus cycle, 250 µg of cloprostenol injection was given intramuscularly to all the animals and assigned to five groups. GI: control, GII: 4 µg of Buserelin, GIII: 20 mg cupric chloride, GIV: 10 mg sodium selenite and GV: copper plus selenium injections were administrated. Whole blood was collected for seven days for hormone assay. Estrus was detected using aproned bucks. Result: There was no significant (p greater than 0.05) difference in estrus response, onset and duration of estrus between groups. The progesterone concentration decreased significantly (p less than 0.05) from day 0 (before PGF2α) to day 1 (after PGF2α) in all groups. There was a significant (p less than 0.05) increase in estradiol and progesterone concentration in the GnRH group compared to other groups. Parenteral administration of copper and selenium did not significantly improve estrus behavior, estradiol and progesterone concentration in Salem black goats. GnRH was once again proved to be effective in the improvement of estrus associated responses in goats.