Safety Of Poultry Products Research Articles

Expression of Genes Related to Meat Productivity, Metabolic and Morphological Significance of Broiler Chickens with the Use of Nutritional Phytochemicals.

The study aimed to analyze gene expression linked to skeletal muscle growth and lipid metabolism in broiler chickens fed with plant extracts. Five groups of chickens were formed: four experimental groups and one control group. The diets of the experimental groups were supplemented with different plant extracts: chicory, St. John's wort, maral root, and creeping thyme, whereas the control group received feed without phytobiotic compounds. Weekly weighings were conducted (n = 36). The chickens were slaughtered at day 26 for tissue sampling of four birds from each group. Gene expression (MYOG, MSTN, FASN) related to muscle growth and fatty acid synthesis was analyzed using the β-actin ACTB gene as a reference. Blood samples were taken at day 35 for biochemical analysis and anatomical dissection was performed. The study revealed that using plant extracts from chicory, thyme, and maral root increased MYOG gene activity by 4.21, 7.45, and 8.93 times, respectively. T. serpyllum extract boosted the MSTN gene by 10.93 times, impacting muscle growth regulation. FASN gene expression for fatty acid synthesis increased significantly by 18.22-184.12 times with plant extracts. The best results regarding meat productivity of chickens were obtained when using R. carthamoides extract. The results of the study will serve as a basis for further development of a phytocomposition designed to increase the meat productivity of broiler chickens in the production of environmentally safe poultry products.

Comparative analyses of bacterial contamination and microbiome of broiler carcasses in wet market and industrial processing environments

The slaughtering environment is crucial for the food hygiene and safety of poultry products. Despite the global dominance of industrial processing, live bird slaughtering in wet markets persists due to cultural, religious, and economic reasons. This study aims to reveal the correlation between hygiene scales in wet markets and bacterial contamination levels on broiler carcasses, with a particular focus on pathobiont transmission risks and microbiome characteristics. Wet markets were categorized based on home-made ratings and the Hygiene and Biosecurity Assessment Tool (HABT). The study assessed total aerobic bacterial (TAB) levels, food spoilage and hygiene indicators (Pseudomonas and E. coli), foodborne pathogen Salmonella, and the microbiome of broiler carcasses, intestinal contents, and slaughtering facilities. Comparative analyses were conducted between market and industrial processing environments. TAB levels on broiler carcasses showed a significant negative correlation with hygiene scores, indicating that both HABT and home-made rating tools effectively assess and improve processing hygiene. Industrial processing consistently reduced bacterial contamination compared to wet markets. Although Salmonella spp. prevalence was lower in market-processed carcasses, the study identified significant cross-transmission of pathobionts and variations in bacterial composition with hygiene improvements. Notably, the microbiome analysis revealed overlaps in amplicon sequence variants (ASVs) between carcasses and contamination vectors, highlighting pathobiont transmission risks. The present study confirmed the scales of hygiene standards among wet markets reflect bacterial contamination on broiler carcasses. Enhancing slaughter practices to meet industrial hygiene standards is essential for reducing the transmission of foodborne pathogens and pathobionts, and improving food safety.

Using tannery wastes in poultry feed: a matter of concern for safe poultry production in Bangladesh

Nowadays tannery waste is a matter of concern because if it used as livestock feed, it could cause health hazards to humans. Therefore, this study was conducted to know the generation rates, utilization, disposal method of tannery solid wastes (TSWs), and inclusion level of it into the poultry feed. Moreover, this study determined the physical and chemical parameters of interest including moisture content, crude protein (CP) levels, and the presence of heavy metals such as chromium (Cr) and lead (Pb) in the poultry feeds that were sold in the studied area. For these purposes, a field survey was conducted with twenty tannery industries. Broiler feed samples were procured from multiple farmers situated in the Savar area of Dhaka. The feed source was classified into two categories, namely registered and unregistered feed mills. According to the study, wet blue trimmings was generated in 3.84% that was used as an ingredient of poultry feed. In addition, 55% of tanneries landfilled their waste, while 30% of them sold it for poultry feed. The utilization of TSWs in poultry feed production in this country was limited to a maximum of 1.314% of the total annual production. Besides, the CP% was determined in the range of 24.24 - 13.32 % and 18.15 - 11.01 % for broiler starters and growers, respectively, where lower CP content was found only in unregistered feed mills. Trace amounts of Cr and Pb were identified in each of the feed samples at very low concentrations. In conclusion, it can be stated that the percentage of tannery solid wastes mixed poultry feed was generated in negligible amounts and the registered companies’ feed was found normal in all aspects of the quality tested in the study. Keywords: Broiler feed, Feed mill, Heavy metal, Physical quality, Tannery solid waste

Supplementation of Biopeptide from Chicken Feet to the Immune System and Growth of Broiler Chicken

The safety of poultry products, especially broiler chickens, can be stated to be relatively low. Antibiotic growth promoter (AGP) is used to improve chicken performance and maintain immunity. The ban on the use of AGP triggers the innovation of other materials to replace the function of AGP. Biopeptide is a solution that can be used. Biopeptides can be made from materials with a high protein content, for example, waste from chicken slaughterhouses. The waste that can be used is chicken feet, biopeptides produced from hydrolytic proteins have antioxidant and antimicrobial properties. These properties can improve the performance and immune system of broiler chickens. The aim of the study was to examine the supplementation of biopeptides from chicken feet on the immune system (total Escherichia coli and internal organ) and the growth of broiler chickens. The research material was 200 day old chickens of broiler strain cobb unsex, which were reared for 42 days of the rearing period. The treatment feeds were P0: basal feed, P1 basal feed + 2% biopeptide, P2: basal feed + 4% biopeptide, P3: basal feed + 6% biopeptide. The collected data were analyzed using analysis of variance with the Tukey test (BNJ). The results showed that biopeptide supplementation from chicken feet had a significant (P<0.05) effect on total Escherichia coli, spleen weight, bursa of Fabricius weight, body weight gain, and feed efficiency for broiler chickens. However, the results of the study had no significant effect (P>0.05) on liver weight and Avian Influenza antibody titers for broiler chickens. Biopeptide supplementation from chicken feet can reduce total Escherichia coli, and spleen weight, and increase the bursa of Fabricius weight, body weight gain, and feed efficiency for broiler chickens. Biopeptide supplementation from chicken feet of 6% gave the best results in body weight gain and feed efficiency.

Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens

The avian oviduct connects to the gastrointestinal tract through cloaca, where it is exposed to pathogenic bacteria from intestinal contents. Therefore, improvement of mucosal barrier function in the oviduct is important for safe poultry production. Lactic acid bacteria are known to contribute to strengthening the mucosal barrier function in the intestinal tract, and a similar effect is expected in the oviduct mucosa of chickens. This study aimed to clarify the effects of vaginal administration of lactic acid bacteria on the mucosal barrier function of the oviduct. White Leghorn laying hens (500-days old) were intravaginally administered 1 mL of Lactobacillus johnsonii suspension (1 × 105 and 1 × 108 cfu/mL: low concentration of Lactobacillus (LL) and high concentration of Lactobacillus (HL) groups, respectively) or without bacteria (control: C group) for 7 d (n = 6). The oviductal magnum, uterus, and vagina were collected for histological observations and mucosal barrier function-related gene expression analysis. Amplicon sequence analysis of oviductal mucus bacteria was also performed. Eggs were collected during the experimental period and their weight was measured. Vaginally administering L. johnsonii for 7 d caused 1) an increase in α-diversity of vaginal mucosa microbiota with an increase in the abundance ratio of beneficial bacteria and a decrease in pathogenic bacteria, 2) enhanced claudin (CLA) 1 and 3 gene expression in the magnum and vaginal mucosa, and 3) a decrease in avian β-defensin (AvBD) 10, 11, and 12 gene expression in the magnum, uterus, and vaginal mucosa. These results suggest that transvaginal administration of L. johnsonii contributes to protection against infection in the oviduct by improving the microflora of the oviductal mucosa and strengthening the mechanical barrier function of the tight junctions. In contrast, transvaginal administration of lactic acid bacteria does not enhance the production of AvBD10, 11, and 12 in the oviduct.

Морфологическая характеристика поверхностной грудной мышцы цыплят- бройлеров кросса «Смена-9» при различных рационах кормления

The article presents the results of studies of the structural features of the superficial pectoral muscle in broiler chickens of the Smena-9 cross with various feeding diets on the 35th day of ontogenesis, carried out within the framework of the thematic plan-tasks for the implementation of research works by state order of the Ministry of Agriculture of Russia on the topic: «Development of theoretical foundations and methodology for the practical use of morphological criteria in assessing the quality and safety of poultry products». It is shown that the studied muscle has a typical bundle structure and is differentiated into a muscular and connective tissue component (endomysium and perimysium). Along with this, signs of increased growth processes were revealed against the background of the use of feed additives, which is manifested by an increase in the thickness of muscle fibers and their bundles and an increase in the number of hemocapillaries compared with the control group. A looser packing of muscle bundles with a better development of perimysium and the formation of white adipose tissue in it in representatives of the experimental groups may indicate a potential improvement in the taste qualities of meat compared to the control. The obtained data can be used as morphological criteria for a comprehensive assessment of poultry meat products during commodity and veterinary-sanitary expertise, and are also important for further developments to improve domestic chicken meat crosses

Особенности строения четырехглавой мышцы бедра цыплят-бройлеров кросса «Смена-9» при различных рационах кормления

The article presents the results of studies of the weight characteristics of broiler chickens of the Smena-9 cross, as well as the morphological features of the quadriceps femoris in these birds on the 35th day of postembryonic ontogenesis with various feeding diets. The work was implemented within the framework of the thematic task plan for the implementation of research works under the state order of the Ministry of Agriculture of the Russian Federation (Agreement No. 082-03-2023- 238 dated 30.01.2023) on the topic: "Development of theoretical foundations and methodology for the practical use of morphological criteria in assessing the quality and safety of poultry products." It is shown that against the background of the use of feed additives, growth processes are stimulated, which is manifested by an increase in the weight of chickens. At the same time, the microstructure of the rectus head of the quadriceps femoris of birds of the control and experimental groups, while maintaining the general plan of structure (the presence of muscular and connective tissue components, bundle organization), becomes unequal. In the experimental group using prebiotic (D1), the loosest packing of muscle fibers in the bundle structure was noted due to the significant development of endomysium and perimysium, which is also characteristic of control samples. In the experimental groups using probiotic and sorbent (D2 and D3, respectively), a dense arrangement of muscle fibers in the bundle structure was revealed, as well as a denser packing of myofibrils in the structure of muscle fibers compared with the control and experimental group D1. At the same time, thickening of the perimisium in the groups and the development of white adipose tissue in it were noted, which may correspond to an improvement in the taste qualities of poultry meat. The obtained results can be used as morphological criteria in the scheme of assessing the quality and safety of meat products. Data on the structural organization of the quadriceps muscle of the new domestic cross expand the theoretical base in the field of the myology of farm birds, including in terms of identifying morphological signs of the realization of genetic potential against the background of the use of different feeding diets in chicken rearing technology, which allows us to quickly assess the effectiveness of chicken rearing technology and predict an increase in productivity.

Analysis of indicators of quality and safety of meat of broiler chickens under the conditions of complex use of symbiotic and biocidal drugs during the entire breeding cycle

The production of ecologically clean poultry meat with high biological value to meet the needs of public demand is the primary goal of the poultry industry. Poultry meat is a good product for a healthy human diet due to its nutritional, dietary, and sensory properties, economic availability, and quick cooking. At the same time, using the latest, effective symbiotic and biocidal drugs to increase the productivity of poultry requires more in-depth research and evaluation of their impact on the final product. Based on the analysis of the results obtained by us, it has been proven that the use of the complex of symbiotic drugs “Biomagn” and “Biozapin” and in combination with the biocidal agents “Diolaid” and “Biolaid” to broiler chickens of the Cobb 500 cross throughout the entire breeding cycle allows obtaining high-quality and safe poultry products (chicken meat), with improved quality indicators and higher biological value. An increase in the protein content in the muscles of experimental groups of birds and a decrease in moisture in them was established, which is a consequence of an increase in dry matter content in this tissue. As a result, in the muscles of broiler chickens of the II research group, an increase in energy value was recorded to a greater extent and markedly, along with an increase in the content of essential bioelements (Calcium, Ferrum, Copper, Cobalt, and Selenium (Р ≤ 0.05)) and the content of vitamins A and B1, which indicates a higher biological (nutritional) value of poultry meat compared to such indicators in poultry I of the experimental and control groups. When determining the safety indicators of the chicken meat of the research groups, it was established that there were no residual amounts of veterinary drugs, pesticides, and aflatoxin B1, and the regulated indicators of toxic elements did not exceed the PAI specified in the regulatory documents. Therefore, the proposed complex of drugs in the scheme of the growing cycle of broiler chickens does not hurt the quality and safety of the obtained products (chicken meat). On the contrary, according to sensory and organoleptic studies, higher organoleptic qualities of the muscle samples of chickens of the I and II experimental groups were proven under the conditions of the use of a complex of symbiotic and biocidal drugs: the values of the highest overall score for tasting the meat broth are higher (Р ≤ 0.05) compared with the indicator in the control group.

Influence of Feeding Wormwood (Artemisia Capillaris) on Quail Meat Productivity

Recently, the requirements for the quality and safety of poultry products have significantly increased, what has notably limited the use of antibiotics in feeding poultry. This is one of the reasons for the growing interest in the use of phytogenic feed additives. We have conducted an experiment to determine the influence of Artemisia capillaris on young quail meat productivity. To carry out the experiment, we have formed five groups, each counting 100 quail heads. During the experiment, which lasted for 35 days, dried powdered wormwood (Artemisia capillaris) in the amount of 0.5%, 1.0 and 1.5% by weight of compound feed was introduced into the feed of birds of the 2nd, 3rd and 4th experimental groups. The experiment showed a positive effect of the studied factor on the body weight of quail; the highest body weight was in quail of the 3rd group - 251.03 g, and the lowest it was in the control group - 238.24 g. Also during the experiment, the lowest feed costs per 1 kg of body weight gain were in quail of the 3rd experimental group, which by this indicator exceeded the control group by 4.91%. Feeding wormwood as a part of the compound feed in the amount of 0.5 and 1.0% of the 2nd and 3rd experimental groups increased the weight of gutted carcass by 0.41-1.67%, while further increase of wormwood content to 1.5% in the feed of the 4th experimental group led to a decrease in the weight of gutted carcass by 0.26% compared to the control group.

Genetic Signature and Serocompatibility Evidence for Drug Resistant Campylobacter jejuni.

Campylobacteriosis, a foodborne illness, is one of the world′s leading causes of gastrointestinal illness. This study investigates the link between human campylobacteriosis and the consumption of potentially contaminated food with Campylobacter jejuni. Three hundred sixty samples were collected from humans, chicken cloaca, raw chicken meat, unpasteurized milk, and vegetables. The chickens were obtained from licensed and non-licensed slaughterhouses, and only the necks and wings were studied. Samples were enriched under microaerobic conditions then cultured on the modified charcoal cefoperazone deoxycholate agar. Bacteria was identified by staining, biochemical testing, and molecular identification by the polymerase chain reaction for the virulence genes; hipO, asp, dnaJ, cadF, cdtA, cdtB, and cdtC. The genomic homogeneity of C. jejuni between human and chicken isolates was assessed by the serological Penner test and the pulse field gel electrophoresis (PFGE). Campylobacter was not detected in the vegetables and pasteurized milk, though, only twenty isolates from chickens and clinical samples were presumed to be Campylobacter based on their morphology. The biochemical tests confirmed that five isolates were C. coli, and fifteen isolates were C. jejuni including two isolates from humans, and the remaining were from chickens. The colonization of C. jejuni in chickens was significantly lower in necks (6.66%) obtained from licensed slaughterhouses compared to those obtained from non-licensed slaughterhouses (33.3%). The antimicrobial susceptibility test showed that all identified C. jejuni isolates were resistant to antibiotics, and the majority of isolates (53.5%) showed resistance against six antibiotics, though, all isolates were resistant to ciprofloxacin, tetracycline, and aztreonam. The Penner test showed P:21 as the dominant serotype in isolates from humans, necks, and cloaca. The serohomology of C. jejuni from human isolates and chicken necks, wings, and cloaca was 71%, 36%, 78%, respectively. The PFGE analysis of the pattern for DNA fragmentation by the restriction enzyme Smal showed a complete genotypic homology of C. jejuni human isolates and chicken necks compared to partial homology with cloacal isolates. The study brings attention to the need for effective interventions to ensure best practices for safe poultry production for commercial food chain supply to limit infection with foodborne pathogens, including Campylobacter.

Dietary chitosan oligosaccharides improves health status in broilers for safe poultry meat production

Feed additives are routinely used for improving poultry health and production worldwide. Chitosan oligosaccharides (COS) is a relatively new feed additive that is a derivative of chitosan, a non-toxic linear polysaccharide with many biological functions. To investigate the efficacy of COS on gut morphology and serum biochemical profile (lipid, protein, glucose, creatinine and enzymes), a total of 180 day-old broiler chicks (n = 45) were allocated into allocated to 1 of 4 groups: control (T0) and treatment groups (T1, T2 and T3) fed basal diet supplemented with increasing amounts of COS for 35 days. Our findings showed that dietary COS supplementation had positive effects on the body weight and body weight gain in the treatment groups. Villus height and width, ratio of villus height and crypts depth, and tunica mucosa thickness of duodenum increased (P > 0.05) in the treatment groups (T1 and T2), whereas crypts depth of duodenum and cecum were decreased in the treatment groups (T1 and T2) compared to the control group; treatment group 3 (T3), however, was almost similar to the control group. In the case of cecum, tunica mucosa thickness, length and width of mucosal folds showed similar results as duodenum. The population and size of intestinal glands and lymphocytic infiltration in the mucosa were increased considerably in the treatment groups (T1 and T2) than control group. Total serum cholesterol, triglyceride, LDL and VLDL cholesterol levels were significantly lowered in the treatment group 2 (T2) than control group or other COS treated groups (P < 0.05), although HDL cholesterol level was significantly (P < 0.05) higher in all of the treatment groups, but substantially higher in the group treatment groups 1 and 3 (T1 and T3) compared to control group. Total serum protein, albumin, and globulin were gradually increased in all of the treatment groups along with the increasing dose rates. The liver and kidney functions (ALT, AST and creatinine) were not significantly (P > 0.05) affected due to COS supplementation. These results suggest that COS has a dose-dependent response to improve gut morphology and health physiology in broilers; low to medium doses are more favorable for safe poultry production as it enhances growth performance, increases villus surface area, reduces undesirable cholesterols and positively affects the blood glucose and protein levels. These results would be helpful in exploring the efficacy of COS as a potential feed additive for safe poultry meat production, which is a time-demanding issue for the poultry industry from the consumers' point of view.

Efficacy of a Biocidal Paint in Controlling Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) and Improving the Quality of Air and Litter in Poultry Houses.

Simple SummaryPoultry meat production and consumption continue to grow worldwide; therefore, the safety of poultry products is a priority for consumers, producers, and governing authorities. Thus, it is essential to find an effective solution to maintain proper hygiene in poultry production. One such tool is a water-based slow-release biocidal paint that contains insecticidal and antimicrobial compounds. In this study it was assumed that biocidal paint could be useful in controlling insect pests such as Alphitobius diaperinus and reducing microbiological contamination of air and litter and have a beneficial effect on microclimate in poultry houses. The results suggest that the tested biocidal paint could be an effective alternative to other insecticides and disinfectants. Additionally, the research is of a practical nature and may be very useful for poultry producers in controlling A. diaperinus populations and maintaining proper hygiene in poultry houses.Effective disinfection and disinsection are the keys to successful operation of modern poultry farms and the safety of poultry products. The cleaning and disinfection of poultry houses are important aspects of farm hygiene management. The correct execution of all steps of cleaning, disinfection, and disinsection procedures and the use of appropriate products are crucial for the prevention and control of zoonotic and animal diseases. In this study it was assumed that a water-based slow-release biocidal paint could be useful in controlling insect pests such as Alphitobius diaperinus and reducing microbiological contamination of air and litter in poultry houses and have a beneficial effect on microclimate in poultry houses. Therefore, the locations of A. diaperinus in the poultry houses, the microbiological contamination of air and litter, as well as the microclimatic conditions in the houses and the physicochemical parameters of the litter were evaluated. The results suggest that the tested biocidal paint could be an effective alternative to other insecticides and disinfectants. Additionally, the research is of a practical nature and may be very useful for poultry producers in controlling A. diaperinus populations and maintaining proper hygiene in poultry houses. Further research is needed.

Stress response modulation: the key to survival of pathogenic and spoilage bacteria during poultry processing.

The control of bacterial contaminants on meat is a key area of interest in the food industry. Bacteria are exposed to a variety of stresses during broiler processing which challenge bacterial structures and metabolic pathways causing death or sublethal injury. To counter these stresses, bacteria possess robust response systems that can induce shifts in the transcriptome and proteome to enable survival. Effective adaptive responses, such as biofilm formation, shock protein production and metabolic flexibility, require rapid induction and implementation at a cellular and community level to facilitate bacterial survival in adverse conditions. This review aims to provide an overview of the scientific literature pertaining to the regulation of complex adaptive processes used by bacteria to survive the processing environment, with particular focus on species that impact the quality and safety of poultry products like Campylobacter spp., Salmonella enterica and Pseudomonas spp.

On the issue of obtaining safe poultry products in veterinary and sanitary terms

The release of good-quality and safe poultry products depends on the sanitary condition, compliance with preventive measures and the development of technologies aimed at improving the safety of the products. Microbiological studies and meat quality indicators were determined using standard methods. A method of cooling in 0.01% solutions of HIT AseptoDes has been developed to control the contamination of the cooling medium and prevent microbial contamination of the surface of poultry carcasses. It has been shown that to reduce microbial contamination and decontamination of the surface of carcasses from Salmonella, 0.02% solutions of the agent can be used with a cooling time of 25 minutes and 0.01% solutions with a cooling time of 35–40 minutes. The quality indicators of meat of carcasses chilled with solutions of the studied product, in comparison with carcasses chilled in ice water, do not differ significantly, with the exception of the acquisition of the surface of the carcasses of a paler color. It was found that after 6 hours of storage, the presence of a residual amount of peracetic acid in washes from carcasses was not detected.

Влияние выпаивания раствора фотосенсибилизатора на микрофлору цыплят-бройлеров для профилактики инфекционных болезней

The aim of the work was to determine the effectiveness of the use of a photosensitizer for the prevention of infectious diseases in industrial poultry farming. The article defines photosensitizers with a description of the reactions they enter into in biological systems. The effectiveness of the photosensitizer «Photoditazine» in the prevention of staphylococcosis of broiler chickens for obtaining high-quality and safe poultry products has been determined. Photosensitizer Photoditazine has bactericidal activity, reducing the overall microbial contamination of the gastrointestinal tract, prevents staphylococcal infection, preserves the weight gain of chickens and ensures the safety of livestock.

Feed additive for quails based on fermented brewer’s spent grain

The paper discusses the effect of a newly developed complex feed additive Grainbiosorb used in complete feed on livability, age of first egg laying, egg productivity, and feed consumption for the production of 1 dozen eggs of laying quails of the Texas white broiler breed. The scientific experiment was carried out in the experimental vivarium of Krasnodar Research Centre for Animal Husbandry and Veterinary Medicine according to the standard technique recommended by the Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of Russian Academy of Sciences, (Sergiev Posad, 2013). The developed complex feed additive Grainbiosorb consists of fermented brewer’s spent grain, a mineral sorbing complex and probiotic microorganisms. Brewer’s spent grain is pre-fermented with probiotic bacterial concentrate, consisting of lactic acid, and propionic acid microorganisms. The use of fermented brewer’s spent grain in combination with a mineral complex can solve the problems of metabolism, digestion, exposure to heavy metals and thereby increase productivity and contribute to obtaining safe poultry products. The livability of the quail population in experimental and control groups was the same and amounted to 90.0%. The quails of the first group laid the first egg at the age of 44 days, while the quails of the second group at the age of 42 days. Gross egg production for the period of the experiment per the initial laying quail and the egg production for the average laying quail increased in the experimental group relative to the control by 3.8%. The intensity of egg production in the experimental group increased by 2.3 abs. % relative to that in the control group. Feed consumption for the production of 1 dozen quail eggs decreased by 5.5% in the experimental group, relative to the control.

Microbiological safety of poultry products in a modified controlled atmosphere during storage

The paper presents the materials for determining the changes in microbiological parameters of poultry products packaged in a modified controlled atmosphere (MCA). It was found that MCA allows stopping the growth of unwanted microbiota, including pathogenic one, in poultry meat and poultry products due to correctly selected components for the controlled atmosphere.

THE SAFETY OF PHOTOSENSITIZERS AS A PREVENTIVE DRUG FOR INDUSTRIAL POULTRY FARMING

The aim of the work was to determine the safety of the use of photosensitizers as a preventive drug for industrial animals.&#x0D; In the theoretical part, the definition of photosensitizers was given with a description of the reactions in which they enter into biological systems, the purpose of the work and the tasks were formulated.&#x0D; In the practical part, the effectiveness of the Photoditazine photosensitizer was determined in order to maintain the well-being of the livestock of industrial birds and to obtain high-quality and safe poultry products. In vivo experiments were conducted to determine the effectiveness of photosensitizers as a preventive drug for production animals. Studies were also conducted to determine the safety of products obtained from experimental animals by feeding their meat to laboratory rats with further determination of their pancreatic elastase concentration by ELISA. For the experiments, non-linear white female rats were selected as the most adequate test object for rapid analysis of the safety of raw materials obtained after the use of photosensitizers.

Cross-Contamination of Enrofloxacin in Veterinary Medicinal and Nutritional Products in Korea.

Poultry meat and eggs are vital sources of protein for human consumption worldwide. The use of several nutritional and medicinal products, including antibiotics, is crucial for efficient and safe poultry production. Accumulation of drug residues in meat and eggs from inappropriate drug use is a major concern to public health. Recently, enrofloxacin was detected (2.4–3.8 ppb) in edible eggs produced in Jeju Island, Korea. Although the farm from which the enrofloxacin-contaminated eggs were collected did not use enrofloxacin-containing products, they reported extensive use of a nutritional product (NPJ). Accordingly, in this study, we investigated whether enrofloxacin contamination had occurred accidentally in various widely used veterinary pharmaceutical products. Enrofloxacin content (4.57–179.08 ppm) in different lots of the NPJ was confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Furthermore, 76 veterinary pharmaceutical products that are widely used in poultry farms in Korea and claim to not contain enrofloxacin were collected and analyzed by LC-MS/MS. Among them, a florfenicol product and a sulfatrimethoprime product were found to contain 3.00 and 0.57 ppm enrofloxacin, respectively. These results suggest that appropriate manufacturing standards are not being followed and that strict monitoring of drug manufacturing is necessary in Korea to avoid drug contamination.

Prospects of betulin application in broiler farming

It is an urgent task today to seek and implement nature-like technologies in broiler production and obtain biologically complete and safe poultry products, thus refusing from antibiotic use in feed. Feed additives based on natural components can be an effective tool for implementation of preventive and therapeutic veterinary measures. The paper presents research study results of application of betulin-based phytobiotics in broiler farming. The tests were carried out on Ross-308 cross-breed broiler chickens within the production cycle at one of the poultry farms of the Sverdlovsk Oblast. Birds of the experimental group received compound feed supplemented with dry betulin at 2.5 mg/kg of live weight from day 21 to day 35 of growing. The introduction of betulin-based feed additive into the diet contributed to increase in live weight gain and 7.6% pectoralis muscle output as compared with the control group. It was established that the phytobiotic consumption resulted in reduced deposition of subcutaneous and abdominal fat, higher biological value of meat by increasing the ash content, improved technological properties of meat due to increasing water-holding capacity of muscle fiber and intensity of formation and maturation of muscle fiber. Histological studies of pancreatic tissue samples from broiler chickens showed increase in the mass of islets of Langerhans and insulin-producing cell complexes. The pancreas was activated due to effects of the betulin-based feed additive. The results obtained indicate that the use of betulin in broiler production is a promising trend.