Sacred Ibis Research Articles

Feeding Habits and Diet Composition of the African Sacred Ibis in Selected Water-Logged Sites in Dar es Salaam, Tanzania

The African Sacred Ibises Threskiornis aethiopicus are generalist birds that feed on many invertebrates and some vertebrates. However, the diet composition of local T. aethiopicus populations in wetland areas in Dar es Salaam Tanzania was unknown. Sacred Ibises from eight water-logged areas in Dar es Salaam City, Tanzania were studied to determine their feeding habits and diet composition using direct observations and molecular analysis of faecal samples. Snails species identified from sites frequented by T. aethiopicus included Lymnaea natalensis (61.62%), Melanoides tuberculatus (20.64%), Bulinus sp. (17.66%) and Anisus sp. (0.08%), while molecular analysis of the birds’ diet revealed presence of mostly non-native invasive molluscs (Physa acuta, Planorbella trivolvis and Radix natalensis), fungus (Candida parapsilosis) and protozoan ciliate (Vorticella striata). There was no significant association between bird and snail abundance (r = 0.2141, p > 0.05, ns) although snail diversity showed strong negative relationship (r = -0.7904, p < 0.05). Our findings indicate that the African Sacred Ibises do not preferentially feed on snails, but do so as part of their broader diet. The results confirm that the African Sacred Ibis is a generalist feeder and therefore, ecological impacts of its feeding strategies particularly on invasive snails should be further investigated.
 Keywords: African Sacred Ibis, feeding, diet, molluscs, generalist

A RETROSPECTIVE ANALYSIS OF THE MORBIDITY AND MORTALITY OF CAPTIVE NORTHERN BALD IBIS (GERONTICUS EREMITA), AFRICAN SACRED IBIS (THRESKIORNIS AETHIOPICUS), AND SCARLET IBIS (EUDOCIMUS RUBER) HOUSED AT THE LONDON ZOO FROM 2000 TO 2020.

Necropsy (n = 144) and medical (n = 121) records of captive northern bald ibis (NBI; Geronticus eremita), African sacred ibis (ASI; Threskiornis aethiopicus), and scarlet ibis (SCI; Eudocimus ruber) housed at the Zoological Society of London's London Zoo (LZ) from 2000 to 2020 were reviewed. Pododermatitis was a common cause of morbidity in all species (79 cases in 247 examinations). Trauma (58 of 144), the majority being caused by suspected collisions with stationary objects in the zoo's habitats, infectious diseases (32 of 144), predominantly valvular endocarditis (10 of 32), and aspergillosis (9 of 32) were major causes of mortality. The odds of a morbidity being related to toxicosis were 4.4 times greater in NBI than for ASI (95% CI, 1.5-13.3; P < 0.05); all cases in the NBI were plumbism. Overall, females of all species had 3.4 times greater odds of undetermined morbidity than males (95% CI, 1.5-7.9; P < 0.05), and the majority (16 of 25) were birds that were thin without an apparent cause. Nestlings had 11.3 times greater odds of nutritional morbidity than adults (95% CI, 1.7-73.0) and 5.5 times greater than juveniles (95% CI, 0.7-41.0; P < 0.05). These data have identified areas that require further study in the population of NBI, ASI, and SCI held at LZ.

Polybrominated diphenyl ethers in the grey-headed gull (Larus cirrocephalus) and African sacred ibis (Threskiornis aethiopicus)

(Left) Ondestepoort landfill showing the birds that forage on the site; (centre right) the grey-headed gull (Larus cirrocephalus) and African sacred ibis (Threskiornis aethiopicus) and (far right) the PBDE levels obtained in feathers of the birds.

Eradication of African sacred ibis (Threskiornis aethiopicus) from South Florida, USA: a collaborative early detection and rapid response case study

Eradication of African sacred ibis (Threskiornis aethiopicus) from South Florida, USA: a collaborative early detection and rapid response case study

Distribution and breeding numbers of a recently split species, the West African Crested Tern Thalasseus albididorsalis

This study investigated the distribution, numbers and conservation threats of the West African Crested Tern, which was recently elevated to full species after it was split from the Royal Tern with an American and African subspecies. In the period 1998–2019, a total of 13 West African coastal islands were identified as breeding localities, stretching from Mauritania to Guinea. All the islands are isolated, usually sandy and subject to erosion. There was great yearly variation in the numbers of breeding pairs within and between sites. A complete census of all breeding locations in 2015 and 2019 resulted in estimates of 79 000 and 77 000 pairs, respectively. The threats identified are predation, human disturbance, nest flooding and loss of breeding habitat as a result of coastal erosion. Predation of eggs and chicks by Sacred Ibises and especially Great White Pelicans may heavily impact on the species’ breeding output. Human disturbance is slight because most of the breeding islands are within protected areas. Flooding of nests has increasingly been observed in recent years, occurring at nine of 11 islands occupied by the terns in 2015. Most islands are subject to erosion, which has resulted in substantial loss of suitable breeding habitat over the 22-year study period. Two important islands have become completely unsuitable. We conclude that West African Crested Terns have an uncertain future. Food shortage resulting from industrial fishing is suspected, and the effects of climate change might negatively impact on habitat suitability and food availability. Monitoring of the total population at three-year intervals is recommended.

The genetic variation of a mitochondrial and two behaviour‐related genes in invasive African Sacred Ibis (Threskiornis aethiopicus) populations in Taiwan

The African Sacred Ibis Threskiornis aethiopicus is an invasive bird species in Taiwan and has expanded its distribution range rapidly over the past three decades. There is limited information available on the invasion process of African Sacred Ibises in Taiwan and its genetic consequences. We investigated whether genetic factors reflected the expansion of African Sacred Ibises and determined the extent to which two behaviour‐associated genes may have facilitated invasion. The dopamine receptor gene (DRD4) and the serotonin transporter gene (SERT) have both been found to be associated with novel‐seeking and bold behaviour in birds. We hypothesized that: (1) selection on temperament traits may determine the polymorphisms in these two genes and (2) the likelihood of dispersal of African Sacred Ibis populations can be explained by the intraspecific variation at these two genes. To detect the signals of population expansion and natural selection, we compared intra‐population variation and inter‐population differentiation of DRD4 and SERT with those of a mitochondrial gene (COX1) and an additional intron. We recovered contrasting patterns of nucleotide variation between the mitochondrial and nuclear genes with no single nucleotide polymorphisms detected in COX1 compared with allelic polymorphism in DRD4 and SERT. Populations showed decreasing genetic diversity at DRD4 as their distance to the initial invasive locality increased, consistent with a rapid expansion from one founder population. However, we found little evidence of selection on DRD4 or SERT, suggesting that these behaviour‐related genes are unlikely to have played a significant role in the successful invasion of the African Sacred Ibises in Taiwan. We detected high levels of genetic variation at these two behaviour‐related genes despite the effects of inbreeding in these invasive birds.

Ten Bird Species, Six Guilds, Three Habitats, and 59 Chlorinated and Brominated POPs: What do 64 Eggs from the Largest Economic Hub of Southern Africa tell us?

There is little information on how POPs in eggs of different terrestrial, wetland, and aquatic birds share a large urban and rural landscape relate. We collected and analysed 64 eggs belonging to ten species of six feeding guilds, and compared organic chlorinated pesticide (OCP), polychlorinated biphenyl (PCB), and brominated flame retardants (BFR) residue concentrations and compositions. The eggs were collected in the Gauteng and the northern part of the Free Sate provinces of South Africa, one of the largest economic hubs in Africa. White-breasted Cormorant and African Darter eggs (at the highest trophic level as large aquatic predators) had the highest ΣOCP and ΣPCB concentrations, and Cape Sparrow and Southern Masked Weaver (granivores) eggs had the lowest concentrations, corresponding to the lowest trophic level in our collection. The highest percentage p,p'-DDT were in eggs of the terrestrial insectivore Crowned Lapwing (24%) and the scavenging African Sacred Ibis (17%), and the lowest in African Darter (1.0%) and White-breasted Cormorant (0.9%) eggs, suggesting that recency of DDT releases in a region cannot be gauged by this metric. African Sacred Ibis and Southern Masked Weaver eggs had the highest ΣBFR concentrations, with Crowned Lapwing, Cattle Egret, and White-breasted Cormorant eggs the least. Based on feeding guilds, the mean ΣPOP concentrations increased from granivore, aquatic omnivore, scavenger, terrestrial insectivore, small aquatic predator, to large aquatic predator. Mean ΣPOP concentrations in eggs increased from terrestrial, to wetland, to aquatic habitat birds. Interesting patterns were observed with multivariate analyses. There were no significant regressions between egg size and any summed POP classes. ΣBFR concentrations were not correlated with ΣOCPs or ΣPCBs. Eggshell thinning of African Darter eggs was associated with p,p'-DDE and ΣPCB suggesting risk. Other metrics also suggest risk. Therefore, different species of terrestrial and aquatic birds from the same area acquire and deposit POPs in different proportions and quantities in their eggs. Trophic levels and habitat explain the overall patterns, but detailed differences were found, some of which we are unable to explain. Based on POPs residues in terrestrial, wetland, and aquatic bird eggs, different POPs classes behave differently in a shared large inland industrial area, complicating deductions about POPs and associated risks based on one or few species.

First confirmed breeding of an invasive alien species in the lagoon of Venice (Italy): the Sacred Ibis &lt;em&gt;Threskiornis aethiopicus&lt;/em&gt;

After more than a decade of regular occurrence, the first confirmed breeding of the Sacred Ibis Threskiornis aethiopicus in the lagoon of Venice (Italy) was observed in 2020, in three sites. The ibises nested in colonies with of one or more species of Ardeidae and Phalacrocoracidae. The colonies were on islets &lt; 2 ha in size, covered with thick vegetation. Nests were placed on the top of bushes or trees, at a height of 1.5-5 m. The colony sites seemed not occupied by adults until the end of February - half of March; laying took place from early May, with hatchings occurring from the end of that month until July. Overall, 22-24 pairs nested in 2020. Birds were observed feeding at saltmarshes, freshwater wetlands and arable fields; a public garbage collection site was also used regularly. Given the high number of suitable nesting and feeding sites, a rapid growth of the Sacred Ibis population is expected in the next years.

A rapid assessment of waterbirds and the mangrove status in the Menabe Antimena Protected Area, Madagascar

Mangroves are of great ecological importance that provide multiple ecosystem services, shelter, and habitat for many threatened waterbird species. The mangroves of the Menabe Antimena Protected Area (MANAP) in western Madagascar are among the most extensive remaining on the island. The remaining dryland forests of the MANAP have been subjected to immense deforestation in recent years. Although remote sensing studies indicate that the mangrove forest loss is considerably lower than the dryland forest loss, little is known about the mangroves’ degradation status. Furthermore, detailed information on bird diversity and numbers is scattered, and previous surveys focused on northern parts of the MANAP, recently designated as the Wetlands of the Tsiribihina RAMSAR site. This study aims to assess bird diversity and abundance, as well as the status of mangroves in the MANAP. We conducted a rapid survey using direct observations at three sites along the coastal regions of the MANAP from 24 Sep–2 Oct 2019. We recorded 71 species of birds in the mangroves and coastal wetlands. High numbers of individuals were counted for several species. Numbers of the Madagascar Plover Charadrius thoracicus and the Madagascar Teal Anas bernieri fulfill criteria for important bird areas at single survey sites and the site is likely to be of importance for Madagascar Sacred Ibis Threskiornis bernieri; these three species are all globally threatened. Mangrove degradation is still limited, but numerous threats to mangroves are present. Our results highlight the importance of the mangroves of the MANAP for several endemic bird species in Madagascar. We provide recommendations for conservation management and future research.

The spreading of the invasive sacred ibis in Italy

The spreading of invasive species in new continents can vary from slow and limited diffusion to fast colonisations over vast new areas. We studied the sacred ibis Threskiornis aethiopicus along a 31-year period, from 1989 to 2019, with particular attention to the first area of release in NW Italy. We collected data on species distribution through observations by citizen science projects, population density by transects with distance method, breeding censuses at colonies, and post breeding censuses at roosts. The birds counted at winter roosts in NW Italy increased from a few tens up to 10,880 individuals in 2019. Sacred ibises started breeding in 1989, with a single nest in north-western Italy. The number of breeders remained very low until 2006, when both overwintering and breeding sacred ibises started to increase exponentially and expand their range throughout northern Italy with isolated breeding cases in central Italy. In 2019, the number of nests had increased to 1249 nests in 31 colonies. In NW Italy, the density of foraging birds averaged 3.9 ind./km2 in winter and 1.5 ind./km2 in the breeding period, with a mean size of the foraging groups of 8.9 and 2.1 birds respectively. Direct field observations and species distribution models (SDM) showed that foraging habitats were mainly rice fields and wetlands. A SDM applied to the whole Italian peninsula plus Sardinia and Sicily showed that the variables best related to the SDM were land class (rice fields and wetlands), altitude, and the temperature seasonality. The areas favourable for species expansion encompass all the plains of Northern Italy, and several areas of Tuscany, Latium, Sardinia, and Apulia.

Capturing the past using DNA from Sacred Ibis Mummies

Capturing the past using DNA from Sacred Ibis Mummies

Mitogenomic diversity in Sacred Ibis Mummies sheds light on early Egyptian practices.

The ancient catacombs of Egypt harbor millions of well-preserved mummified Sacred Ibis (Threskiornis aethiopicus) dating from ~600BC. Although it is known that a very large number of these ‘votive’ mummies were sacrificed to the Egyptian God Thoth, how the ancient Egyptians obtained millions of these birds for mummification remains unresolved. Ancient Egyptian textual evidences suggest they may have been raised in dedicated large-scale farms. To investigate the most likely method used by the priests to secure birds for mummification, we report the first study of complete mitochondrial genomes of 14 Sacred Ibis mummies interred ~2500 years ago. We analysed and compared the mitogenomic diversity among Sacred Ibis mummies to that found in modern Sacred Ibis populations from throughout Africa. The ancient birds show a high level of genetic variation comparable to that identified in modern African populations, contrary to the suggestion in ancient hieroglyphics (or ancient writings) of centralized industrial scale farming of sacrificial birds. This suggests a sustained short-term taming of the wild migratory Sacred Ibis for the ritual yearly demand.

LA IMPORTANCIA ECONÓMICA Y TEOLÓGICA DEL LUGAR DE NACIMIENTO DE EL IBIS

The article gives a new explanation for the masses of sacred ibises and their remains inside the Egyptian ibis burial places (Ibiotapheia) especially in Ptolemaic times. We find no traces of a continuous flow of pilgrims visiting sanctuaries of Thot and offering single ibis mummies to venerate Thot privately. The many new Ptolemaic nourishing places of the ibis (Ibiotropheia/Ibion) are economically closely connected to the distribution of new Fields of the ibis, sold by the administration also to foreigners like kleruchoi-soldiers. This happened in combination with the installation of many new cult places and cult statues of Ibis- and Baboon-gods, all maintained by an Ibion-organisation. Every newly founded local ibion in the Nile valley delivered selected sacred ibises to a new local Greek-Egyptian Hermaion where they fulfilled roles of gods during feast days and oracle procedures. Collection of dead ibises from the Ibion and their deification, then the mass burial of all animal remains and finally the rebirth of young sacred ibises inside an Ibion secured the immortality of the Ibis-god. This Ibis- god protected all over Egypt the pharaoh’s New Year feast and his enthronisation corresponding to the mythological role of the ibises as supporters of Thot during the first creation.

Correction: The Sacred Ibis debate: The first test of evolution.

[This corrects the article DOI: 10.1371/journal.pbio.2005558.].

The Sacred Ibis debate: The first test of evolution.

In 1798, Napoleon Bonaparte’s army invaded Egypt, returning with many treasures including large numbers of Sacred Ibis mummies. The ancient Egyptians revered the ibis and mummified literally millions of them. The French naturalist Georges Cuvier used these mummies to challenge an emerging idea of the time, namely Jean-Baptiste Lamarck’s theory of evolution. Cuvier detected no measurable differences between mummified Sacred Ibis and contemporary specimens of the same species. Consequently, he argued that this was evidence for the “fixity of species.” The “Sacred Ibis debate” predates the so-called “Great Debate” between Cuvier and Geoffroy Saint-Hilaire and the publication of Darwin’s On the Origin of Species five decades later. Cuvier’s views and his study had a profound influence on the scientific and public perception of evolution, setting back the acceptance of evolutionary theory in Europe for decades.

Trace Element Contamination in Tissues of Four Bird Species from the Rift Valley Region, Ethiopia.

Concentrations of ten trace elements (Hg, As, Cd, Pb, Co, Cr, Cu, Ni, Se and Zn) were determined in different tissues (liver, kidney, muscle, heart and brain) of African sacred ibis (Threskiornis aethiopicus), Hamerkop (Scopus umbretta), marabou stork (Leptoptilos crumeniferus) and great white pelican (Pelecanus onocrotalus) inhabiting the Ethiopian Rift Valley region. There were differences in trace element patterns among the bird species. Significantly (p < 0.05) higher concentrations of Cd (5.53µg/g dw ± 2.94) in kidney and Hg (0.75µg/g ww ± 0.30) in liver were observed in the great white pelican compared to the other species, and liver concentrations of these two elements showed positive correlations with trophic level. Concentrations of toxic elements (As, Cd, Pb and Hg) in liver were below their respective toxicological thresholds, indicating that the data may provide baseline information for future studies.

Susceptibility and Status of Avian Influenza in Ostriches.

The extensive nature of ostrich farming production systems bears the continual risk of point introductions of avian influenza virus (AIV) from wild birds, but immune status, management, population density, and other causes of stress in ostriches are the ultimate determinants of the severity of the disease in this species. From January 2012 to December 2014, more than 70 incidents of AIV in ostriches were reported in South Africa. These included H5N2 and H7N1 low pathogenicity avian influenza (LPAI) in 2012, H7N7 LPAI in 2013, and H5N2 LPAI in 2014. To resolve the molecular epidemiology in South Africa, the entire South African viral repository from ostriches and wild birds from 1991 to 2013 (n = 42) was resequenced by next-generation sequencing technology to obtain complete genomes for comparison. The phylogenetic results were supplemented with serological data for ostriches from 2012 to 2014, and AIV-detection data from surveillance of 17 762 wild birds sampled over the same period. Phylogenetic evidence pointed to wild birds, e.g., African sacred ibis (Threskiornis aethiopicus), in the dissemination of H7N1 LPAI to ostriches in the Eastern and Western Cape provinces during 2012, in separate incidents that could not be epidemiologically linked. In contrast, the H7N7 LPAI outbreaks in 2013 that were restricted to the Western Cape Province appear to have originated from a single-point introduction from wild birds. Two H5N2 viruses detected in ostriches in 2012 were determined to be LPAI strains that were new introductions, epidemiologically unrelated to the 2011 highly pathogenic avian influenza (HPAI) outbreaks. Seventeen of 27 (63%) ostrich viruses contained the polymerase basic 2 (PB2) E627K marker, and 2 of the ostrich isolates that lacked E627K contained the compensatory Q591K mutation, whereas a third virus had a D701N mutation. Ostriches maintain a low upper- to midtracheal temperature as part of their adaptive physiology for desert survival, which may explain the selection in ratites for E627K or its compensatory mutations-markers that facilitate AIV replication at lower temperatures. An AIV prevalence of 5.6% in wild birds was recorded between 2012 and 2014, considerably higher than AIV prevalence for the southern African region of 2.5%-3.6% reported in the period 2007-2009. Serological prevalence of AI in ostriches was 3.7%, 3.6%, and 6.1% for 2012, 2013, and 2014, respectively. An annual seasonal dip in incidence was evident around March/April (late summer/autumn), with peaks around July/August (mid to late winter). H5, H6, H7, and unidentified serotypes were present at varying levels over the 3-yr period.

Conflicting traffic: characterization of the hazards of birds flying across an airport runway

AbstractBird–aircraft collisions cost millions of dollars to aviation globally and cause deaths. We designed and tested a protocol to study the hazards to aircraft from birds flying across runways where aircraft rotate and climb during take‐off. We recorded birds and flight height of birds flying across runway 03L at OR Tambo International Airport, South Africa. A total of 7,938 birds of pigeon size or larger crossed a 400 m length of runway during 14 h and 15 min, a rate of 8.8 birds per minute; there were 200 aircraft taking off during this period. The biggest bird–aircraft collision hazard is posed by African Sacred Ibis and Grey‐headed Gull. Respectively, these species contribute a mean of 111 kg per 10 min and 47.2 kg per 10 min biomass flying across the runway. We identify possible management options to reduce the hazard of bird–aircraft collisions. Our survey protocol and data treatment is easy to use, will add additional and important definition to existing activities to reduce bird–aircraft collisions and can provide comparable hazard information to aerodrome authorities and pilots.

Redke vrste ptic v Sloveniji v letu 2014 – Poročilo Nacionalne komisije za redkosti /Rare birds in Slovenia in 2014 – Slovenian Rarities Committee's Report

This report by the Slovenian Rarities Committee presents records of rare bird species in Slovenia in 2014, with some addenda for previous years. The numbers in brackets refer to the number of records (first number) and individuals (second number) recorded between 1 Jan 1950 and 31 Dec 2013. Since 1 Jan 2013, submission to the Committee has been required for 37 additional species, 17 of which are regional rarities. Records of these species are not numbered, since records from previous years were not collected by the Committee. One new species was added to category C5: Sacred Ibis Threskiornis aethiopicus. One species, Spotted Dove Streptopelia chinensis, was added to category E. Breeding was confirmed for Muscovy Duck Cairina moschata and Northern Bobwhite Colinus virginianus; the two species were thus transferred to subcategory E*. Other notable observations were the third and fourth records of Black Vulture Aegypius monachus, fourth and fifth records of Gannet Morus bassanus, fourth record of Pomarine Skua Stercorarius pomarinus and fourth record of Richard’s Pipit Anthus richardi. The flocks of four Gannets and 23 Flamingos Phoenicopterus roseus have been the largest recorded in Slovenia to date. The list of birds recorded in Slovenia (as of 31 Dec 2014) contains 385 species (369 in category A, 7 in category B, 9 exclusively in category C; 4 species are both in categories A and C). Category D contains 6 species, while category E contains 34, two of which are classified into subcategory E*. These two categories are not part of the list.

Radiocarbon dating of Sacred Ibis mummies from ancient Egypt

Sacred Ibis (Threskiornis aethiopicus) were widespread in Egypt until the eighteenth century. Today the species is extinct in modern Egypt but millions of mummified specimens are scattered geographically in dedicated Ibis burial sites throughout the country. Ibises were regarded as physical manifestations of the god Thoth and worshiped by the ancient Egyptians. A small number of Sacred Ibis were chosen as ‘sacred animals’, based on physical markings, and were reared for the temples. However, the majority of the mummified Sacred Ibis were ‘votive animals’ that were given as offerings to the deities by pilgrims, and then buried in catacombs associated with the temple. Their supply became an industry that is thought to have flourished from the Late Period, well into the Roman Period (c. 664 BC to AD 350). Dating of the Sacred Ibis mummies, as well as other mummified animal specimens, has been based on archaeological evidence such as the age of catacombs, the design of enclosures and the shape of the mummy containers (pottery jars, wooden chests or stone boxes). Here we present the first ages of a selection of Sacred Ibis mummies using 14C methods in order to establish how closely they match the archaeological chronology. Dates are reported from museum samples provenience from Saqqara, Roda and Thebes. Our 14C radiocarbon results date the Ibis mummies between c. 450 and 250cal BC and represent a short period of time. Those dates are falling from the Late Period to the Ptolemaic Period at maximum. Surprisingly, none of the samples were dated to the Roman era.