Concentrations Of Saccharin Research Articles

Effect of deposition potential and saccharin addition on structural, magnetic and magnetoresistance characteristics of NiCoFeCu films

Abstract NiCoFeCu films were electrodeposited on Ti substrates at different deposition potentials and different concentrations of saccharin added to solution. Compositional analysis showed that although Ni was the highest concentration in solution at low potentials of −1.0 V and −1.5 V, the Ni content was lower than the Co content in the films. Anomalous co-deposition behaviour of iron group metals was observed. When the deposition potential increased to −2.0 V and −2.5 V, the Ni content of films increased while the Co, Fe and Cu content decreased. In the case of saccharin addition to the solution, there is a slight change in the film content. All films have face-centred cubic structure. Structural analysis clearly showed that the potential has a significant effect on the texture degree of the films, since the crystal texture changed from (111) to (220) with increasing potential. The surface morphology of the films was observed to be affected by the deposition potential and saccharin concentration. For the magnetic analysis, saturation magnetisation, M s value gradually decreased from 905 to 715 emu/cm3 with the variation of film content caused by the increase of the potential from −1.0 V to −2.5 V. And, a slight increase in M s was detected with the addition of saccharin. Besides, the longitudinal and transverse magnetoresistance magnitudes increased from ∼2.5 % to 7.0 % with increasing deposition potential and all films exhibit anisotropic magnetoresistance. Films with desired magnetic properties can be obtained for potential use as magnetic materials in electronics such as magnetoresistive devices.

Sweetener System Intervention Shifted Neutrophils from Homeostasis to Priming.

Non-nutritive sweeteners (NNS) are part of personalized nutrition strategies supporting healthy glycemic control. In contrast, the consumption of non-nutritive sweeteners has been related to person-specific and microbiome-dependent glycemic impairments. Reports on the effects of NNS on our highly individual cellular immune system are sparse. The recent identification of taste receptor expression in a variety of immune cells, however, suggested their immune-modulatory relevance. We studied the influence of a beverage-typical NNS system on the transcriptional profiling of sweetener-cognate taste receptors, selected cytokines and their receptors, and on Ca2+ signaling in isolated blood neutrophils. We determined plasma concentrations of saccharin, acesulfame-K, and cyclamate by HPLC-MS/MS, upon ingestion of a soft drink-typical sweetener surrogate. In an open-labeled, randomized intervention study, we determined pre- versus post-intervention transcript levels by RT-qPCR of sweetener-cognate taste receptors and immune factors. Here we show that the consumption of a food-typical sweetener system modulated the gene expression of cognate taste receptors and induced the transcriptional regulation signatures of early homeostasis- and late receptor/signaling- and inflammation-related genes in blood neutrophils, shifting their transcriptional profile from homeostasis to priming. Notably, sweeteners at postprandial plasma concentrations facilitated fMLF (N-formyl-Met-Leu-Phe)-induced Ca2+ signaling. Our results support the notion of sweeteners priming neutrophils to higher alertness towards their adequate stimuli.

Dissecting operant alcohol self-administration using saccharin-fading procedure.

Although alcohol use disorder is a complex human pathology, the use of animal models represents an opportunity to study some aspects of this pathology. One of the most used paradigms to study the voluntary alcohol consumption in rodents is operant self-administration (OSA). In order to facilitate the performance of this paradigm, we aim to describe some critical steps of OSA under a saccharin-fading procedure. We used 40 male Wistar rats to study the process of acquiring the operant response through a saccharin-fading procedure under a fixed ratio (FR1) schedule of reinforcement. Next, we analyze the alcohol introduction and concentration increase, the effect of an alcohol deprivation, and the analogy between this paradigm with the Drinking in the Dark-Multiple Scheduled Access paradigm. During alcohol concentration increase, animals reduced their lever presses in accordance with the increase in alcohol concentration. On the contrary, the consumption measured in g·kg-1 BW showed a great stability. The lever presses pattern within operant session changes with the introduction of different alcohol concentrations: at higher alcohol concentrations, animals tended to accumulate most of their presses in the initial period of the session. We show the utility of fading with low concentrations of saccharin and the evolution of the operant response through the different concentrations of alcohol. Taken together, our results aimed to dissect the acquisition and maintenance of OSA behavior as well as other related variables, to facilitate the understanding and performance of this paradigm.

Assessment of Artificial Sweeteners as Wastewater Co-Tracers in an Urban Groundwater System of Mexico (Monterrey Metropolitan Area)

Contamination from wastewater infiltration, typically from leaky sewers, poses a threat to urban groundwater resources. Artificial sweeteners (Asws), used as sucrose substitutes in many products of daily consumption, are released into groundwater systems and may be used as tracers of wastewater in urban groundwater environments, because most of these compounds are discharged directly into sewer systems. Here, for the first time, we investigated the occurrence of Asws in an urban groundwater system in Mexico. Artificial sweetener concentrations of acesulfame (ACE), aspartame (ASP), cyclamate (CYC), saccharin (SAC), and sucralose (SUC) were tested in 42 production wells in the Monterrey Metropolitan Area (MMA). The detection frequencies of quantified Asws observations were in the order ACE (57%) > SUC (54%) > SAC (7%), with SUC being the most abundant Asws, with concentrations below the quantification limit (BQL) of 2.9 µg/L, followed by ACE (BQL 0.73 µg/L) and SAC (BQL 1.4 µg/L). ASP and CYC were not detected at any sampling site. Considerable Asws ingestion amongst the MMA population is the main input source of Asws into the city’s wastewater network, percolating into the urban groundwater system due to leaky sewers. Our work shows that the application of Asws as wastewater tracers (SUC and ACE) effectively determines wastewater sources affecting urban groundwater.

Further disentangling the motivational processes underlying benzodiazepine hyperphagia

In addition to their well-known anxiolytic functions, benzodiazepines produce hyperphagia. Previously, we reported that the benzodiazepine, chlordiazepoxide (CDP), increased consumption of both normally-preferred and normally-avoided taste stimuli during long-term (1 h) tests, primarily through changes in licking microstructure patterns associated with hedonic taste evaluation, whereas there was little effect on licking microstructure measures associated with post-ingestive feedback. In this study, we further examined the hedonic and motivational specificity of CDP effects on ingestive behavior. We tested brief access (15 s) licking responses for tastants spanning all taste qualities after treatment with either CDP (5 or 10 mg/kg) or the non-benzodiazepine anxiolytic, buspirone (1.5 or 3 mg/kg). A between-subjects, counterbalanced design compared the CDP or buspirone effects on licking responses for water and a range of weak to strong concentrations of NaCl, Q-HCl, citric acid, MSG, saccharin, and capsaicin under water-restricted (23 h) conditions; and sucrose, saccharin, and MSG under water-replete conditions. In a dose dependent manner, CDP increased licking for taste stimuli that were normally-avoided after saline treatment, with a notable exception observed for the trigeminal stimulus, capsaicin, which was not affected at any concentration or drug dose, suggesting a taste-specific effect of CDP on orosensory processing. Under water-replete conditions, CDP dose-dependently increased licking to normally-accepted concentrations of sucrose, saccharin, and MSG. There was no effect of either drug on licks for water under either water-restricted or water-replete conditions. Buspirone slowed oromotor coordination by increasing brief interlick intervals, but it did not affect licking for any concentrations of the tastants. Overall, these results indicate that benzodiazepines selectively enhance the hedonic acceptance of gustatory orosensory stimuli, independent of general anxiolytic or oromotor coordination effects, or physiological states such as thirst.

Synthetic Sugar Substitutes Alter Intestinal Epithelial Cell Proliferation and Differentiation in a Cell-Line Dependent Manner In Vitro

ObjectivesThe acute and long-term effects of synthetic sugar substitutes have yet to be fully determined. Importantly, their interactions with the gut epithelium are poorly understood. The objective of this study was to examine the impact of two synthetic sugar substitutes on intestinal epithelial cells in terms of 1) cellular toxicity, 2) effects on cell proliferation, and 3) effects on cellular differentiation.MethodsGut epithelial cells (n = 3/group) were challenged for 3 hours with varying concentrations of saccharin and sucralose, to determine cellular toxicity thresholds, as assessed by MTT assay. Three morphologically distinct epithelial cell lines (HT-29, HT-29MTX, and T84) were also challenged (n = 3/group) with the maximum concentrations of these compounds that did not induce toxicity to evaluate cell proliferation over a 96-hour period. RNA was isolated from synthetic sugar substitute-challenged cells and analyzed for epithelial cell differentiation-related gene expression via CDX2 by qRT-PCR, and normalized to B2M, an epithelial cell reference gene.ResultsCellular toxicity assays indicated that 10% w/v sweetener was the maximum level tested that avoided evidence of cellular toxicity. When comparing distinct epithelial cell lines, HT-29MTX and T84 displayed significant differences in cell proliferation when exposed to saccharin (HT29-MTX, P < 0.01) and sucralose (T84, P < 0.01), demonstrating increased cell proliferation, relative to unchallenged controls. Finally, CDX2 expression, a marker of cellular differentiation, was significantly increased in the presence of both saccharin (5% w/v) and sucralose (10% w/v) in HT-29 cells, relative to unchallenged controls (P < 0.05).ConclusionsThese findings demonstrate that exposure to high concentrations of synthetic sugar substitutes alter proliferation and differentiation of gut epithelial cell lines in vitro, in a cell line-dependent manner. Additional research is necessary to determine whether these interactions also occur in vivo.Funding SourcesThis work was funded by the University of Nevada, Reno Department of Nutrition, the College of Agriculture, Biotechnology, & Natural Resources, the Vice President for Research and Innovation, and the Nevada Agricultural Experiment Station.

Sex differences in sucrose reinforcement in Long-Evans rats

BackgroundThere are sex differences in addiction behaviors. To develop a pre-clinical animal model to investigate this, the present study examined sex differences in sucrose taking and seeking using Long-Evans rats.MethodsFive experiments were conducted using separate groups of subjects. The first two examined sucrose or saccharin preference in two-bottle home cage choice tests. Experiment three assessed sucrose intake in a binge model with sucrose available in home cage bottles. Experiments four and five utilized operant-based procedures. In experiment four rats responded for sucrose on fixed and progressive ratio (FR, PR) schedules of reinforcement over a range of concentrations of sucrose. A final component of experiment four was measuring seeking in the absence of sucrose challenged with the dopamine D1 receptor antagonist SCH23390. Experiment five assessed responding for water on FR and PR schedules of reinforcement.ResultsWhen accounting for body weight, female rats consumed more sucrose than water; but there was no sex difference in saccharin preference over a range of saccharin concentrations. When accounting for body weight, females consumed more sucrose than males in the binge model, and only females increased binge intake over 14 days of the study. Females responded at higher rates for sucrose under both FR and PR schedules of reinforcement. Females responded at higher rates in extinction (seeking); SCH23390 reduced sucrose seeking of both females and males. Females responded at higher rates for water on FR and PR schedules than males, although rates of responding were low and decreased over sessions.ConclusionsAcross bottle-choice, binge intake, and operant procedures, female Long-Evans rats consumed more sucrose and responded at higher rates for sucrose. Although females also responded more for water, the vigor of responding did not explain the consistent sex difference in sucrose taking and seeking. The sex difference in sucrose taking was also not explained by sweet preference, as there was no sex difference in saccharin preference. These data provide a pre-clinical model to further evaluate sex differences in addiction behaviors and manipulations designed to reduce them.

Non-Nutritive Sweeteners in Human Amniotic Fluid and Cord Blood: Evidence of Transplacental Fetal Exposure.

This study aimed to investigate human fetal exposure to non-nutritive sweeteners (NNS) by analyzing amniotic fluid and umbilical cord blood. Concentrations of four NNS (acesulfame-potassium [ace-K], saccharin, steviol glucuronide, and sucralose) were measured in amniotic fluid (n = 13) and cord blood samples (n = 15) using liquid chromatography-mass spectrometry. Amniotic fluid samples were obtained for research purposes at the time of term elective cesarean birth or clinically indicated third trimester amnioreduction at Mercy Hospital for Women (Melbourne, Australia). All except four women were in the fasting state. Cord blood samples were obtained from an independent cohort of newborns whose mothers were enrolled in a separate clinical trial at the National Institutes of Health. Ten of 13 amniotic fluid samples contained at least one NNS (ace-K, saccharin, steviol glucuronide, and/or sucralose). Maximum amniotic fluid NNS concentrations of ace-K, saccharin, steviol glucuronide, and sucralose were 78.9, 55.9, 93.5, and 30.6 ng/mL, respectively. Ace-K and saccharin were present in 100% and 80% of the cord blood samples, with maximal concentrations of 6.5 and 2.7 ng/mL, respectively. Sucralose was not detected and steviol glucuronide was not measurable in any of the cord blood samples. Our results provide evidence of human transplacental transmission of NNS. Based on results predominantly obtained from rodent models, we speculate that NNS exposure may adversely influence the offsprings' metabolic health. Well-designed, prospective clinical trials are necessary to understand the impact of NNS intake during pregnancy on human development and long-term health. · NNS consumption during pregnancy has increased in recent years.. · Maternal NNS intake during pregnancy is associated with preterm birth and higher infant weight gain in epidemiologic studies.. · In rodents, in utero NNS exposure induces metabolic abnormalities in mothers and their offspring, alters offspring gut microbiota composition, and promotes sweet taste preference in adulthood.. · It is presently unknown whether and to what degree maternal NNS ingestion in humans leads to direct in utero exposure.. · This study provides the first evidence of in utero NNS exposure in humans and highlights the urgent need to investigate clinical consequences of early life NNS exposure on metabolism, weight, taste preference, and general health..

Optimization of a saccharin molecularly imprinted solid-phase extraction procedure and evaluation by MIR hyperspectral imaging for analysis of diet tea by HPLC

Saccharin was determined based on a new molecularly imprinted solid-phase extraction (MISPE) procedure. The polymer was synthesized with a hybrid monomer of metacrylic acid and 3-amino propril tetraethoxysilane and saccharin as template. After the synthesis, the saccharin removal from the MIP was verified by the UV analysis of the solutions used in the template removal procedure, as well as by the direct MIP analysis using FTIR hyperspectral image and chemometrics. The residual saccharin concentrations observed in the image analysis revealed a narrow concentration distribution consistent with a homogenous material. The MISPE was performed with homemade cartridges containing 200 mg of the MIP. The results obtained with standards and diet tea samples confirmed high affinity, adsorption capacity and selectivity of the MIP. The MISPE cartridge exhibited recoveries of 100 ± 3% in six extraction cycles. The diet tea analysis showed a significant reduction of the interferences, which can considerable simplifies the HPLC-UV analysis.

Evaluation of Saccharin and Resveratrol as Extrinsic Markers of Small-Quantity Lipid-Based Nutrient Supplement Consumption in Healthy Women.

ABSTRACTBackgroundDietary supplements, like small-quantity lipid-based nutrient supplements (SQ-LNS), are used in intervention programs to prevent undernutrition among women and young children in low-income countries. An objective marker is needed to track consumption of supplements to evaluate the effectiveness of these programs.ObjectiveThe aim of this study was to evaluate saccharin and resveratrol as potential adherence markers for tracking recent consumption of a single serving of SQ-LNS in women.MethodsForty-seven healthy nonpregnant women 18–45 y of age were assigned to consume a single dose of SQ-LNS (20 g) containing either 10 mg sodium saccharin or 5 mg trans-resveratrol, under supervision. On the day before and for 2 d following SQ-LNS consumption, urine samples were collected each day for 24 h as 3 consecutive 4-h collections and one 12-h overnight collection. Urinary concentrations of saccharin and trans-resveratrol-3-O-sulfate, a resveratrol metabolite, were measured by ultra-high-performance liquid chromatography interfaced to a mass spectrometer with electrospray ionization [UHPLC-(ESI-)MS/MS]. Urinary concentrations (μmol/L urine) of saccharin and trans-resveratrol-3-O-sulfate were plotted against time, and receiver operating characteristic (ROC) curves were used to determine the discriminative capacity of each compound, at each post-consumption time point compared with baseline, to detect recent consumption of SQ-LNS. Cutoff values to differentiate supplement consumption from nonconsumption of each marker were developed using the closest-to-(0,1)-corner cut-point approach.ResultsForty-five participants were included in the analysis. Urinary concentrations of saccharin and trans-resveratrol-3-O-sulfate increased within 4 h of SQ-LNS consumption. Urinary concentration cutoff values for saccharin (13.4 µmol/L) and trans-resveratrol-3-O-sulfate (0.7 µmol/L) allowed for 78% and 89% sensitivity, respectively, and 100% specificity in detecting consumption of SQ-LNS within the first 12 h after consumption.ConclusionsUrinary concentrations of saccharin and trans-resveratrol-3-O-sulfate reflect consumption of SQ-LNS containing those compounds during the first 12 h post-consumption with high sensitivity and specificity in healthy women and may be useful objective adherence markers for tracking consumption of SQ-LNS.

Alteration of unfolded protein responses and autophagy signaling represented the molecular basis underlying saccharin toxicity to Drosophila (Diptera: Drosophilidae).

The purpose of this study was to develop a new control method for Drosophila using saccharin sodium dihydrate (saccharin), an artificial sweetener that is safe for humans and the environment, and to elucidate its mode of action. In this study, we confirmed that saccharin can dose-dependently inhibit the development of or kill vinegar flies (VFs) and spotted wing Drosophila (SWDs). In addition, we found that low concentrations of saccharin induced a similar effect as starvation in Drosophila, whereas high concentrations of saccharin induced changes in the unfolded protein response (UPR) and autophagy signaling that were unlike starvation and inhibited development or killed the VF and the SWD by performing real-time quantitative polymerase chain reaction analyses. Spinosad is a widely used plant protection agent for SWD control. When saccharin was cotreated with 0.25-1.0 ppm spinosad, an additive insecticidal activity was observed only at high concentrations of saccharin. However, when saccharin was cotreated with 2.0 ppm spinosad, an additive insecticidal activity was observed at low concentrations of saccharin. Taken together, alteration of UPR and autophagy signaling represented the molecular basis underlying saccharin toxicity to Drosophila and concurrent spraying of an insecticide with saccharin could enhance the insecticidal activities.

Resveratrol and Saccharin As Adherence Markers Discriminate Between Daily, Intermittent, or Non-consumption of Small-Quantity Lipid-Based Nutrient Supplements

ObjectivesAn objective adherence marker is needed to track consumption of nutrient supplements in nutrition intervention trials and programs targeted to young children and women of reproductive age in low-income countries. Here, resveratrol and saccharin are evaluated as adherence markers for tracking short-term intermittent or daily consumption of a small-quantity lipid-based nutrient supplement (SQ-LNS) in US women in a ten-day controlled feeding study. MethodsSixty-three women were randomly assigned to receive daily for 10 d either: SQ-LNS containing trans-resveratrol (10 mg) and sodium saccharin (10 mg) (SQ-LNS/RS); 2) control SQ-LNS without the markers (SQ-LNS/C); or 3) alternating SQ-LNS/RS or SQ-LNS/C to simulate intermittent consumption. Urine concentrations of saccharin and trans-resveratrol-3-O-sulfate were measured in pooled urine samples collected 0–4 h post-consumption of the assigned SQ-LNS product on study days 4, 5, 9 and 10. Cutoff values were determined for urinary concentrations of each compound for classifying participants as consumers or non-consumers of SQ-LNS/RS. Then, participants were classified as daily, intermittent or non-consumers of SQ-LNS/RS based on their overall consumption pattern (number of days classified as a consumer during the 10-d period). ResultsThe cutoff value for urinary trans-resveratrol-3-O-sulfate (0.80 μmol/L) classified participants correctly as consumers or non-consumers in 97% of samples; the cutoff value for urinary saccharin (6.92 μmol/L) classified participants correctly in 93% of samples. Overall, 95% of daily, 95% of intermittent, and 100% of control group participants were classified correctly into their consumption pattern based on urinary trans-resveratrol-3-O-sulfate concentrations during the 10-d period; and 75% of daily, 95% of intermittent, and 100% of control participants were classified correctly based on urinary saccharin concentrations. ConclusionsThe results suggest that urinary concentrations of saccharin and trans-resveratrol-3-O-sulfate may be useful for qualitative assessment of daily vs. intermittent vs. non-consumption of SQ-LNS; further work is needed to assess the usefulness of the markers in the intended target populations. Funding SourcesBill & Melinda Gates Foundation

Artificial Sweeteners Negatively Regulate Pathogenic Characteristics of Two Model Gut Bacteria, E. coli and E. faecalis.

Artificial sweeteners (AS) are synthetic sugar substitutes that are commonly consumed in the diet. Recent studies have indicated considerable health risks which links the consumption of AS with metabolic derangements and gut microbiota perturbations. Despite these studies, there is still limited data on how AS impacts the commensal microbiota to cause pathogenicity. The present study sought to investigate the role of commonly consumed AS on gut bacterial pathogenicity and gut epithelium-microbiota interactions, using models of microbiota (Escherichia coli NCTC10418 and Enterococcus faecalis ATCC19433) and the intestinal epithelium (Caco-2 cells). Model gut bacteria were exposed to different concentrations of the AS saccharin, sucralose, and aspartame, and their pathogenicity and changes in interactions with Caco-2 cells were measured using in vitro studies. Findings show that sweeteners differentially increase the ability of bacteria to form a biofilm. Co-culture with human intestinal epithelial cells shows an increase in the ability of model gut bacteria to adhere to, invade and kill the host epithelium. The pan-sweet taste inhibitor, zinc sulphate, effectively blocked these negative impacts. Since AS consumption in the diet continues to increase, understanding how this food additive affects gut microbiota and how these damaging effects can be ameliorated is vital.

Effect of short-term intake of four sweeteners on feed intake, solution consumption and neurotransmitters release on mice.

This study focused on the effect of short-term intake of sweeteners on feed intake, solution consumption and neurotransmitters release on mice. The results showed that the free drinking of 10mM sucralose solution, 100mM maltose solution, 3mM saccharin solution and 3g/L stevioside solution for 32days will not affect the normal development of the body weight and feed intake of the mice. The consumption of maltose solution was significantly higher than that of the other sweeteners. The leptin and insulin levels increased significantly after the short-term intake of these four sweeteners. The dopamine (DA) content in the whole brain of the mice increased significantly only in the maltose group. These results indicate that the short-term intake of the preferred concentrations of maltose, stevioside, sucralose and saccharin will not affect the body weight and feed intake of the mice. Mice prefer maltose solution to other sweeteners solutions. The 100mM maltose solution and 3mM saccharin solution could result in the oxidative stress on mice after 32days' short-term intake. Compared with other sweeteners, only sugars that could be broken down into small molecules of glucose might have a positive effect on dopamine levels.

Distribution of Four Artificial Sweeteners in Water Treatment and Water Supply System in City H of Zhejiang Province

Taking the sewage and drinking water treatment system of two cities in Zhejiang Province as the main research target, a systematic investigation on the common artificial sweeteners was carried out by solid phase extraction-high performance liquid chromatography (SPE-HPLC). The results showed that SPE-HPLC can accurately determine micro acesulfame (ACE), saccharin (SAC), aspartame (ASP), and neotame (NEO) in water. The detection limits were 57, 120, 170, and 47 μg·L-1, and the quantification limits were 190, 400, 567, and 157 μg·L-1, respectively. After optimization of the solid phase extraction procedure when using PWAX columns, the recoveries of ACE and SAC were enhanced to 86.5% and 97.7%; however, it slightly decreased to 66.5% for NEO. The occurrence rates of ACE, SAC, and NEO in domestic sewage were 100.0%, and it was only 33.3% for ASP. The highest concentrations of four aforementioned artificial sweeteners in domestic sewage were 0.83, 4.52, 1.22, and 7.57 μg·L-1, respectively. The highest concentrations of acesulfame, saccharin, aspartame, and neotame in source water were 22.94, 39.17, 0.73, and 8.92 μg·L-1, respectively, and detection rates were 72.7%, 90.9%, 18.2%, and 90.9%, respectively. Most artificial sweeteners were removed by the prozone process; the removal efficiencies of ACE, SAC, and NEO ranged from 67.7% to 100.0%, and the removal efficiencies of ACE, SAC, and NEO by the advanced treatment process ranged from 73.7% to 100.0%, which effectively reduced the risk of drinking water quality. The four artificial sweeteners were not detected in the water supply network.

Modification of Morphological, Structural and Optical Properties of CBD-Based Growth of PbS Films on Glass Substrates by Addition of Saccharin

Saccharin which so-called as benzoic sulfimide is an artificial sweetener. In this paper, nanostructured lead sulfide (PbS) thin films were produced in the presence of additive known as saccharin via chemical bath deposition (CBD). Effect of the saccharin content on the morphological, optical and structural properties of the films has been investigated. Lead sulfide (PbS) films that included additive were prepared at five different concentrations of saccharin (from 0 to 5%). The fabricated samples were characterized by surface roughness, scanning electron microscopy (SEM), X-Ray diffraction and UV-vis spectroscopy. It was found that the presence of saccharin highly affected the optical and structural properties of PbS thin films. According to the X-Ray diffraction results, crystallite size values of the films decreased from 12.88 to 5.25 nm with increasing of saccharin content. UV-vis measurements showed that both the band gap and transmission properties of the films increased as a result of increasing saccharin concentration. Further, Surface roughness measurements exhibited that average surface roughness was diminished with increasing saccharin content. As a result, it has been found that the presence of saccharin in growth solution played a significant mission in adjusting the main physical properties of the samples.

Safety and efficacy of sodium saccharin when used as a feed flavour for piglets, pigs for fattening, calves for rearing and calves for fattening.

Sodium saccharin is intended to be used as a sweetener in feed and water for drinking for piglets, pigs for fattening and veal calves. The Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) considers the proposed maximum use level of 150 mg sodium saccharin/kg feed as safe for calves and pigs for fattening. For piglets (sucking and weaned piglets), a lower level of 100 mg sodium saccharin/kg complete feed is considered safe. The corresponding maximum safe concentrations in water for drinking are 30 mg/L for piglets and 50 mg/L for pigs for fattening, respectively. The maximum safe concentrations of sodium saccharin in feed and water for drinking are derived under the premise that only one source, feed or water for drinking, contains the additive. The FEEDAP Panel concludes that no concern for the consumer would result from the use of sodium saccharin in feed and water for drinking at the dose considered safe for the target species. The precautions for handling the product proposed by the applicant are considered to be sufficient to ensure user safety. The FEEDAP Panel concludes that the use of sodium saccharin at the dose considered safe for target species is unlikely to have detrimental effects on the terrestrial and freshwater compartments. The high mobility and relative persistence of saccharin and the high persistency of its degradation product 4‐hydroxysaccharin indicate that groundwater contamination above 0.1 μg/L is likely to occur. Since the function of sodium saccharin in feed for the target species is essentially the same as that in food, the FEEDAP Panel concludes that no demonstration of efficacy is necessary.

Genotoxic Activity of Saccharin, Acesulfame-K, Stevia and Aspartame-Acesulfame-K in Commercial Form

Genotoxic activity of various concentrations of saccharin, acesulfame-K, aspartame-acesulfame-K and stevia in their commercial form was assessed. Human lymphocytes were exposed to different concentrations of saccharin, acesulfame-K, aspartame-acesulfame-K and stevia for 2 h and then subjected to alkaline comet assay system. Saccharin and the aspartame-acesulfame-K combination showed significant genotoxic activity (P<0.0001). Concentrations 0.5% of acesulfame-K and stevia did not induce significant genetic damage particularly stevia possesses antigenotoxic activity at 5%, 0.5% and 0.05%. Saccharin and the combination of aspartame-acesulfame- K have genotoxic activity and represent a genetic risk for consumers. Acesulfame-K and stevia are harmless and stevia even possesses antigenotoxic activity at concentrations below 5%.

Physicochemical characteristics, textural properties, and sensory attributes of low‐calorie cereal bar enhanced with different levels of saccharin during storage

A low-calorie cereal bar was developed using various concentrations of saccharin (0.005. 0.0075, and 0.01 wt/wt) and its physicochemical characteristics, textural properties, and sensory qualities during storage were evaluated. Water activity and textural properties were significantly lower in the cereal bar prepared with saccharin than in the bar prepared with starch syrup (control). However, the low-calorie cereal bar formulated with a high concentration of saccharin had sensory attributes significantly different from that of control. In addition, the lower moisture content, water activity, and textural properties, and sensory parameters of this bar deteriorated throughout the storage period. However, microbial properties were unobservable during the storage period. Therefore, saccharin could be suitable for making low-calorie cereal bars, with 0.01% being the optimal concentration. Practical applications Nowadays, consumers are more aware about their health and prefer sugar-reduced or sugar-free products. Therefore, sugar replacement products are in demand in markets. In this study, a low-calorie cereal bar was formulated using saccharin and its physiochemical properties were evaluated. This information would be useful, especially, to individuals who want to consume sugar-free products.

Influences of Saccharin on Electrochemical Behavior of Nickel Electrodeposition

Electrodeposition of Ni was performed in acid Watt's solutions. Saccharin concentrations were varied in order to analyze its influence on the electrochemical behaviors of Ni deposition. Investigations were conducted using CHI660D, by methods of linear potential sweep, cyclic voltammetry and A. C. Impedance. Results indicate that with the increase of saccharin concentration, the cathodic polarization, apparent activation energy and the electrochemical reaction resistance of Ni electrodeposition increase. The exchange current density decreases with the increase of saccharin concentration. The electrodeposition of Ni is a mixing process controlled by electrochemical polarization and concentration polarization. The change of saccharin concentration does not affect the electrodeposition mechanism of Ni. But there is an obvious inhibition and blocking effect of saccharin on nickel electrodeposition. When the concentration of saccharin is 4g/L, its adsorption capacity in electrode is close to saturation. The saccharin concentration should be less than 4g/L.