sIgA Concentration Research Articles

Lactiplantibacillus plantarum L47 and inulin affect colon and liver inflammation in piglets challenged by enterotoxigenic Escherichia coli through regulating gut microbiota

IntroductionInfection by pathogenic bacteria during weaning is a common cause of diarrhea and intestinal inflammation in piglets. Supplementing the diet with synbiotics is beneficial for animal health. The strain of Lactiplantibacillus plantarum L47 (L47) isolated in our lab exhibited good probiotic properties when combined with inulin. Here, the effectiveness of combining L47 and inulin (CLN) in protecting against enterotoxigenic Escherichia coli (ETEC) induced colon and liver inflammation in weaned piglets was evaluated.MethodsTwenty-eight piglets aged 21 days were randomly assigned into 4 groups: CON (control), LI47 (oral CLN culture fluid, 1010 CFU/d of L47 and 1 g/d of inulin), ECON (oral ETEC culture fluid, 1010 CFU/d), and ELI47 (oral CLN and ETEC culture fluid). After 24 days, the colon and liver samples were collected for further analysis.Results and discussionCLN alleviated colon damage caused by ETEC challenge, as evidenced by an increase of colonic crypt depth, mRNA expression of tight junction Claudin-1 and Occludin, GPX activity, the concentration of IL-10 and sIgA (p < 0.05). Moreover, there was a decrease in MDA activity, the load of E. coli, the concentration of LPS, gene expression of TLR4, and the concentration of TNF-α and IL-6 (p < 0.05) in colonic mucosa. Additionally, CLN counteracted liver damage caused by ETEC challenge by modulating pathways associated with immunity and disease occurrence (p < 0.05).ConclusionSupplementing with CLN alleviated colon inflammation induced by ETEC challenge by decreasing the E. coli/LPS/TLR4 pathway and regulating hepatic immune response and disease-related pathways, suggesting that CLN could protect intestinal and liver health in animals.

Effect of Fiber Drink Consumption Containing Glucomannan and Isomaltooligosaccharide on Digesta Profile and Gut Microbiota

Dietary fibers have long been recognized for their numerous health benefits, including supporting gut health, aiding in weight management, and reducing the risk of cardiovascular disease. Glucomannan, derived from konjac and porang tubers, has gained attention for its diverse health-promoting properties. However, its application in beverages is limited due to its high viscosity. Combining glucomannan with isomaltooligosaccharide (IMO) is suggested to synergistically enhance functionality, particularly improving gastrointestinal health. This study investigates the functional effects of porang glucomannan and isomaltooligosaccharide-containing fiber drinks (GIFD) on body weight, feed intake, digesta profiles (digesta weight, moisture content, pH) and gut microbiota using in vivo method. Twenty-four male Sprague Dawley rats were divided into 4 groups, including control, inulin, and 2 doses of glucomannan and isomaltooligosaccharide fiber drink (GIFD). Rats were fed with AIN-93M. Adaptation was carried out for 5 days followed by 28 days of intervention. Results indicate that GIFD consumption led to reduced feed intake, likely due to increased satiety effects and glucomannan’s gel-forming ability, resulting in lower body weight gain compared to other groups. While GIFD did not significantly alter digesta weight and moisture contents, it significantly decreased pH values. Analysis of short-chain fatty acids (SCFAs) showed a shift in propionic acid levels, suggesting a beneficial effect on weight control. Moreover, GIFD treatment reduced abundance percentages of enteric bacteria like E. coli and Clostridium, possibly due to increased SCFA production. However, GIFD consumption did not significantly alter sIgA concentration, although there was a trend towards increased levels in GIFD-2, suggesting potential immunomodulatory effects. Overall, it was indicated that porang glucomannan and IMO-containing fiber drinks has the potential to be used as beverages to support body weight regulation and to help maintaining digestive tract health. HIGHLIGHTS Glucomannan and isomaltooligosaccharide-containing fiber drinks (GIFD) reduced feed intake. Consumption of GIFD induced the shift of molar proportion of SCFAs suggesting beneficial health effect. GIFD treated groups showed lower abundance percentages of coli and Clostridium compared to control. GIFD is potential to be developed as weight management product. GRAPHICAL ABSTRACT

COMPARATIVE CHARACTERISTICS OF METHODS FOR THE PREVENTION OF DENTAL CARIES IN CHILDREN OF EARLY CHILDHOOD

The high prevalence and intensity of early childhood caries in Ukraine and in the world indicate insuffi cient attention of practical dentistry to the prevention of dental caries in young children. The development of adapted prevention programs considering the risk factors for the development of the disease remains relevant.Aim of the study. To compare the eff ectiveness of methods of pre- and postnatal prevention of early childhood dental caries based on correction of the microbial environment of the oral cavity and macro- and microelemental nutrition.Material and methods. The work was carried out within the framework of the research work of the Department of Pediatric Dentistry of the Bukovinian State Medical University «Development of methods of prevention and treatment of major dental diseases in children, taking into account the risk factors of their development» (DR # 0121U110122). The prenatal method of dental caries prevention was applied to a group of pregnant women (30 persons) whose average age was (25,17±1,05) years. It included the use of Elevit Pronatal and Iodomarin 200, one tablet daily, starting from the second trimester ofpregnancy, and a course of local probiotic therapy with BioGaya ProDentis chewable tablets, used for 20 days in the second trimester of pregnancy and 20 days before delivery. As a method of postnatal prevention of dental caries in children, a preventive complex was used, including the use of the probiotic BioGaya ProDentis and the vitamin and mineral complex Supervit. The course lasted three weeks twice a year. The preventive complex was applied to a group of children aged 2-3 years. The number of children in the control group was 30. The mean age of the group was (2.51±1.30) years. The eff ectiveness of preventive measures was evaluated by the prevalence and intensity of caries of primary teeth («kpv» and «kpppv»), the state of oral hygiene (M. E. Kuzmina index), the content of phosphate ions, inorganic and ionized calcium in the oral fluid of children, the content of mutans streptococci in the oral cavity and the concentration of sIgA in the oral fl uid of children. Statistical processing of the study results was performed using standard methods of variation. The study complied with all bioethical norms (conclusion of the Bioethics Commission of the Bukovinian State Medical University No. 4 dated 15.12.2022).Results of the study. The use of preventive complexes for early childhood caries, based on the supplementation of macro- and microelements and the infl uence on the microbial landscape of the oral cavity, are eff ective methods of prevention of caries of temporary teeth in children, which is confi rmed by a decrease in the prevalence and intensity of dental caries in children in the dynamics of their observation. Prenatal measures are more eff ective in the prevention of early childhood caries in children, as they optimize the processes of primary mineralization of hard dental tissues and thus strengthen them, reducing the risk of dental caries. Postnatal use of the drugs has a slightly lower preventive eff ect on early childhood caries, while it has a positive eff ect on the processes of secondary mineralization of tooth enamel, as it was found that the level of phosphate ions in the oral fl uid of children under these conditions increased by 23.72 % (p<0.05), and the level of total calcium decreased by 15.84 % and its free fraction by 25.86 % (p<0.05).Conclusions. Our results suggest that both methods of prevention of early childhood caries are eff ective, but prenatal prophylaxis is clinically more eff ective with respect to the hard tissues of temporary teeth in children, due to its eff ect on the processes of primary mineralization of enamel. Postnatal prophylactic measures have a positive eff ect on the secondary mineralization of the hard tissues of temporary teeth and the primary mineralization of permanent teeth and will have a clinical result in the long term.

Establishment and Validation of Fecal Secretory Immunoglobulin A Measurement for Intestinal Mucosal Health Assessment in Wild Lemurs.

The measurement of biomarkers in blood and excreta can enable immune status assessment and provide prognostic information on individual health outcomes. In this respect, the fecal measurement of secretory immunoglobulin A (sIgA), the primary mammalian antibody for mucosal defense, has recently received increased interest in a few anthropoid primates, but a fecal sIgA assay for use in strepsirrhine primates has not yet been reported. Here, we develop and analytically validate a cost-effective in-house sandwich enzyme immunoassay for the extraction and measurement of sIgA in feces of redfronted lemurs (Eulemur rufifrons). We also tested a simple method for sIgA extraction that can be used under remote field conditions and undertook experiments to assess the robustness of sIgA concentrations to variation in processing and storage conditions of fecal extracts. Our analytical validation revealed that the assay recognizes immunoreactive sIgA in redfronted lemur feces, that sIgA can be measured accurately with no potential interference from the fecal matrix, and that assay reagents and performance are highly stable over time. The field-friendly extraction procedure produced sIgA results strongly correlated with those generated by a standard laboratory extraction method. Short-term storage at room temperature resulted in a slight decline in sIgA concentrations, whereas freezing extracts at -20°C kept sIgA levels stable for at least 3 months. Longer-term storage of >5 months, however, led to a significant decline of sIgA concentrations. Multiple freeze-thaw cycles did not affect sIgA levels. This study, therefore, provides the basis for measuring fecal sIgA in lemurs and possibly other strepsirrhines. When samples are processed properly and stored frozen, and when sIgA analysis can be performed within 3 months upon sample collection, fecal sIgA measurements can become a valuable tool for monitoring aspects of immunity and health in both zoo-housed and wild-living lemurs.

Akkermansia muciniphila Promotes SIgA Production and Alters the Reactivity Toward Commensal Bacteria in Early-Weaned Piglets

BackgroundSecretory IgA (SIgA) is the first line of defense in protecting the intestinal epithelium against pathogenic bacteria, regulating gut microbiota composition, and maintaining intestinal homeostasis. Early weaning strategies may disrupt SIgA levels in piglet intestines, causing a decline in immune response and early weaning stress. However, the specific microbial mechanisms modulating SIgA in early-weaned piglets are not well understood. ObjectivesWe hypothesized that Akkermansia muciniphila increases intestinal SIgA production in the early-weaned piglets. MethodsFecal SIgA levels, SIgA-coated bacteria abundance, and fecal metagenomes were compared between 6 Huanjiang miniature (HM) and 6 Duroc×Landrace×Yorkshire (DLY) early-weaned piglets to identify bacterial species involved in SIgA modulation. Four bacterial species were investigated using 5 groups (Control, A. muciniphila, L. amylovorus, L. crispatus, and L. acidophilus) of male specific pathogen-free C57BL/6J mice, weaned 3 wk postbirth (n = 8/group). Subsequently, 10-d-old Landrace×Yorkshire (LY) piglets were randomly assigned to 3 groups (Control, 109A. muciniphila, and 108A. muciniphila) (n = 10/group) to evaluate the effect of orally administered A. muciniphila on intestinal SIgA production and microbial composition. ResultsHM early-weaned piglets showed significantly higher SIgA levels [7.59 μg/mg, 95% confidence interval (CI): 3.2, 12, P = 0.002] and SIgA-coated bacteria abundance (8.64%, 95% CI: 3.2, 14, P = 0.014) than DLY piglets. In the mouse model, the administration of A. muciniphila significantly increased SIgA levels (3.50 μg/mg, 95% CI: 0.59, 6.4, P = 0.018), SIgA-coated bacteria abundance (9.06%, 95% CI: 4, 14, P = 0.018), and IgA+ plasma cell counts (6.1%, 95% CI: 4.3, 8, P = 0.005). In the pig experiments, the oral administration of A. muciniphila to LY piglets significantly enhanced intestinal SIgA concentrations (4.22 μg/mg, 95% CI: 0.37, 8.5, P = 0.034) and altered the SIgA-coated bacterial landscape. ConclusionsEarly intervention with A. muciniphila in nursing piglets can increases intestinal SIgA production and alter the reactivity toward commensal bacteria upon early weaning.

Airway epithelium damage in acute respiratory distress syndrome

BackgroundThe airway epithelium (AE) fulfils multiple functions to maintain pulmonary homeostasis, among which ensuring adequate barrier function, cell differentiation and polarization, and actively transporting immunoglobulin A (IgA), the predominant mucosal immunoglobulin in the airway lumen, via the polymeric immunoglobulin receptor (pIgR). Morphological changes of the airways have been reported in ARDS, while their detailed features, impact for mucosal immunity, and causative mechanisms remain unclear. Therefore, this study aimed to assess epithelial alterations in the distal airways of patients with ARDS.MethodsWe retrospectively analyzed lung tissue samples from ARDS patients and controls to investigate and quantify structural and functional changes in the small airways, using multiplex fluorescence immunostaining and computer-assisted quantification on whole tissue sections. Additionally, we measured markers of mucosal immunity, IgA and pIgR, alongside with other epithelial markers, in the serum and the broncho-alveolar lavage fluid (BALF) prospectively collected from ARDS patients and controls.ResultsCompared to controls, airways of ARDS were characterized by increased epithelial denudation (p = 0.0003) and diffuse epithelial infiltration by neutrophils (p = 0.0005). Quantitative evaluation of multiplex fluorescence immunostaining revealed a loss of ciliated cells (p = 0.0317) a trend towards decreased goblet cells (p = 0.056), and no change regarding cell progenitors (basal and club cells), indicating altered mucociliary differentiation. Increased epithelial permeability was also shown in ARDS with a significant decrease of tight (p < 0.0001) and adherens (p = 0.025) junctional proteins. Additionally, we observed a significant decrease of the expression of pIgR, (p < 0.0001), indicating impaired mucosal IgA immunity. Serum concentrations of secretory component (SC) and S-IgA were increased in ARDS (both p < 0.0001), along other lung-derived proteins (CC16, SP-D, sRAGE). However, their BALF concentrations remained unchanged, suggesting a spillover of airway and alveolar proteins through a damaged AE.ConclusionThe airway epithelium from patients with ARDS exhibits multifaceted alterations leading to altered mucociliary differentiation, compromised defense functions and increased permeability with pneumoproteinemia.

The effect of 12-week high-dose Colostrum Bovinum supplementation on immunological, hematological and biochemical markers in endurance athletes: a randomized crossover placebo-controlled study.

Bovine colostrum (COL) is assumed to be one of the strongest natural immune stimulants. Regular ingestion of COL may contribute to improved immune response in athletes exposed to high training loads. Twenty-eight endurance-trained males aged 31.1 ± 10.2 years (body mass 81.9 ± 9.0kg; height 1.82 ± 0.06m) completed this randomized double-blind placebo(PLA)-controlled crossover study aimed at investigating the effect of 12-week COL supplementation (25gCOL·day-1) on resting (REST), exercise-induced (POST-EX), and short-term post-exercise recovery (REC; 1h after test exercise) changes in selected saliva and blood immunoglobulins (Ig), white blood cell (WBC) count and differential; as well as blood hematological, nutritional status and muscle damage indices. The protocol assumed 4 study visits - before/after supplementation with COL (COLPRE and COLPOST ) and PLA (PLAPRE and PLAPOST ). During testing sessions, incremental rowing test to exhaustion and swimming-specific performance test were introduced as exercise stimuli. At COLPOST visit the secretory IgA (SIgA) concentration in saliva was significantly higher at POST-EX and REC compared to REST (p<0.05). COL supplementation had no effect on blood IgA, IgE, IgD, IgG, and IgM concentrations. Furthermore, after COL supplementation decrease of hematocrit at REC (p<0.05) was revealed. 12-week supplementation with 25 gCOL·day-1 in endurance-trained male athletes resulted in a favorable increase in post-exercise concentration of salivary SIgA. COL seems to be a potential stimulator of local immune defense after exercise-induced homeostasis disturbances. Nevertheless, the lack of effect on blood markers indicates the need for further research in the area of mechanisms underlying the effect of the supposed COL immunological capacity.

Feeding Docosahexaenoic Acid and Arachidonic Acid during Suckling and Weaning Contributes to Oral Tolerance Development by Beneficially Modulating the Intestinal Cytokine and Immunoglobulin Levels in an Allergy-Prone Brown Norway Rat Model

BackgroundSuckling and weaning arachidonic acid (ARA) + docosahexaenoic acid (DHA) supplementation promoted oral tolerance (OT) development in pups, however, the effect of it on the intestine to promote OT development remains unknown. ObjectiveWe aimed to explore the impact of this supplementation on intestinal fatty acid composition, structure, and indicators that are supportive of OT development. MethodsAllergy-prone Brown Norway dams were randomly assigned to a control (0% ARA, 0% DHA) or ARA + DHA diet (0.45% ARA, 0.8% DHA) during suckling (0–3 wk). At weaning (3–8 wk), offspring were randomly assigned to a control (0% ARA, 0% DHA) or ARA + DHA diet (0.5% ARA, 0.5% DHA). At 3 wk, offspring in each group received an oral gavage of sucrose or ovalbumin (OVA) solution for five consecutive days. At 7 wk, all offspring received an intraperitoneal OVA injection. At 8 wk, offspring were terminated to evaluate jejunum morphology and measure mucosal food allergy-related secretory immunoglobulin A (sIgA) and cytokines, ileum phospholipid and triglyceride fatty acid compositions, and fecal calprotectin. ResultsWeaning ARA + DHA resulted in a higher percentage of DHA in ileum phospholipids and triglycerides (both P < 0.001), without affecting the percentage of ARA. Despite no lasting effect of suckling ARA + DHA on the DHA content in ileum phospholipids, a programming effect was found on the allergy-related intestinal immune profile [higher concentrations of mucosal IL-2 (P = 0.049) and sIgA (P = 0.033)]. OVA treatment resulted in a lower concentration of mucosal IL-6 (P = 0.026) regardless of dietary interventions. Offspring fed ARA + DHA during suckling and/or weaning had a higher concentration of mucosal transforming growth factor-beta (TGF-β) after OVA treatment but this was not observed in offspring fed control diets during suckling and weaning (P = 0.04). ConclusionsEarly life dietary ARA + DHA supplementation to allergy-prone rats enhanced the DHA concentration in intestinal phospholipids (weaning period) and increased the mucosal sIgA, IL-2, and TGF-β levels (suckling and weaning period), indicating its ability to create a tolerogenic intestinal environment to support OT development.

Chlorogenic Acid Enhances the Intestinal Health of Weaned Piglets by Inhibiting the TLR4/NF-κB Pathway and Activating the Nrf2 Pathway.

Chlorogenic acid (CGA) is a natural polyphenol with potent antioxidant and anti-inflammatory activities. However, the exact role of it in regulating intestinal health under oxidative stress is not fully understood. This study aims to investigate the effects of dietary CGA supplementation on the intestinal health of weaned piglets under oxidative stress, and to explore its regulatory mechanism. Twenty-four piglets were randomly divided into two groups and fed either a basal diet (CON) or a basal diet supplemented with 200 mg/kg CGA (CGA). CGA reduced the diarrhea rate, increased the villus height in the jejunum, and decreased the crypt depth in the duodenum, jejunum, and ileum of the weaned piglets (p < 0.05). Moreover, CGA increased the protein abundance of Claudin-1, Occludin, and zonula occludens (ZO)-1 in the jejunum and ileum (p < 0.05). In addition, CGA increased the mRNA expression of pBD2 in the jejunum, and pBD1 and pBD2 in the ileum (p < 0.05). The results of 16S rRNA sequencing showed that CGA altered the ileal microbiota composition and increased the relative abundance of Lactobacillus reuteri and Lactobacillus pontis (p < 0.05). Consistently, the findings suggested that the enhancement of the intestinal barrier in piglets was associated with increased concentrations of T-AOC, IL-22, and sIgA in the serum and T-AOC, T-SOD, and sIgA in the jejunum, as well as T-AOC and CAT in the ileum caused by CGA (p < 0.05). Meanwhile, CGA decreased the concentrations of MDA, IL-1β, IL-6, and TNF-α in the serum and jejunum and IL-1β and IL-6 in the ileum (p < 0.05). Importantly, this study found that CGA alleviated intestinal inflammation and oxidative stress in the piglets by inhibiting the TLR4/NF-κB signaling pathway and activating the Nrf2 signaling pathway. These findings showed that CGA enhances the intestinal health of weaned piglets by inhibiting the TLR4/NF-κB pathway and activating the Nrf2 pathway.

The Role of Ramadan Fasting on Secretory IgA and Statherin Levels in Individuals with Dental Caries

Background: Tooth decay happens when bacteria in the mouth create acid from carbohydrates that can be fermented. Along with factors related to the person (like genetics or oral hygiene habits) and saliva, this process leads to cavities. During Ramadan fasting, when eating habits change, there could be effects on health, particularly on the salivary biomarkers that are important for dental well-being. Objectives: This cross-sectional study aimed to detect the activity and concentrations of salivary levels of secretory immunoglobulin A and Statherin during and after Ramadan fasting and to evaluate the association between these biomarkers with dental caries. Methods: The study comprised 40 individuals, aged 20-25 years, diagnosed with dental caries. Participants were assessed for periodontal parameters using the Plaque Index and the Gingival Index. Saliva samples were collected during the fourth week of Ramadan fasting and two weeks after Ramadan fasting. The concentrations of both secretory IgA and Statherin were measured in salivary samples using Enzyme-Linked Immunosorbent Assay . Results: A significant decrease in the mean concentration of secretory immunoglobulin A was observed during Ramadan fasting (2.14±0.21 ng/L) compared to post-fasting (3.34±0.35 ng/L) (p=0.001). However, there was a non-significant difference (p=0.05) in statherin levels between the fasting state (2.25±0.18 ng/L) and the post-fasting state (2.85±0.22 ng/L). No statistically significant difference was found concerning both the Plaque Index and the Gingival Index within fasting and post-fasting state. Conclusion: Low concentration of sIgA and Statherin during Ramadan fasting may indicate altered salivary gland activity or systemic response to fasting, potentially affecting oral health.

Heyndrickxia coagulans strain SANK70258 suppresses symptoms of upper respiratory tract infection via immune modulation: a randomized, double-blind, placebo-controlled, parallel-group, comparative study.

Probiotic consumption strongly influences local intestinal immunity and systemic immune status. Heyndrickxia coagulans strain SANK70258 (HC) is a spore-forming lactic acid bacterium that has immunostimulatory properties on peripheral tissues. However, few reports have examined the detailed effectiveness of HC on human immune function and its mechanism of action. Therefore, we conducted a randomized, double-blind, placebo-controlled, parallel-group study to comprehensively evaluate the effects of HC on immunostimulatory capacity, upper respiratory tract infection (URTI) symptoms, and changes in intestinal organic-acid composition. Results of a questionnaire survey of URTI symptoms showed that runny nose, nasal congestion, sneezing, and sore throat scores as well as the cumulative number of days of these symptoms were significantly lower in the HC group than in the placebo group during the study period. Furthermore, the salivary secretory immunoglobulin A (sIgA) concentration was significantly higher, and the natural killer (NK) cell activity tended to be higher in the HC group than in the placebo group. In addition, we performed an exposure culture assay of inactivated influenza virus on peripheral blood mononuclear cells (PBMCs) isolated from the blood of participants in the HC and placebo groups. Gene-expression analysis in PBMCs after culture completion showed that IFNα and TLR7 expression levels were significantly higher in the HC group than in the placebo group. In addition, the expression levels of CD304 tended to be higher in the HC group than in the placebo group. On the other hand, the HC group showed a significantly higher increase in the intestinal butyrate concentration than the placebo group. HC intake also significantly suppressed levels of IL-6 and TNFα produced by PBMCs after exposure to inactivated influenza virus. Collectively, these results suggest that HC activated plasmacytoid dendritic cells expressing TLR7 and CD304 and strongly induced IFNα production, subsequently activating NK cells and increasing sIgA levels, and induced anti-inflammatory effects via increased intestinal butyrate levels. These changes may contribute to the acquisition of host resistance to viral infection and URTI prevention.

Effect of aromatic massage on brain waves and physiological indices of older adults.

Massage and aromatherapy are frequently used by older adults as alternative interventions to enhance immunity and induce relaxation. This pilot study evaluated the effect of massage therapy with oil and aromatherapy alone and in combination using objective biological indices. Twenty-eight participants recruited by convenience sampling included adults aged between 25 and 65 years (Group 1), elderly individuals over 65 years without nursing care (Group 2), and older adults over 65 needing long-term nursing support (Group 3). A multiple-group pretest-post-test design was employed, and the effect among the three groups was compared. Interventions included: (i) oil massage therapy; (ii) aromatherapy; and (iii) aroma oil massage therapy. Each therapy session lasted 5 min, with 3 min of observation before and after the session and 10 min interval between sessions. Group 3 omitted one therapy (2: aromatherapy) to reduce their physical burden. An electroencephalogram (EEG) was recorded for α, β, and θ activities of brain waves. EEG data were collected at three points: before, during, and after each treatment. Salivary secretory immunoglobulin A (s-IgA) concentration, oxygen saturation (SPO2), and pulse rate were measured before and after each session. Across all therapy modalities, there was a noticeable increase in the α wave, indicative of relaxation, during the treatment. Significant differences were observed before and during the oil massage in both Group 1 and Group 2. Aromatherapy demonstrated a significant difference before and during treatment in Group 1. Among the biological parameters, s-IgA levels indicated no significant changes. The pulse rate decreased with oil massage. Significant differences were noted before and after therapy in all cases for SPO2 and in Group 2 for pulse rate. Three therapies induced EEG and physiological changes in the adult group and older adults without nursing care. However, these effects are limited in older adults requiring nursing care.

52 Alleviating antinutritional effects of high soybean meal diets for nursery pigs through multienzyme and acidifier supplementation

Abstract This study evaluated the effect of multienzyme and acidifier supplementation in high soybean meal (SBM) diets on performance and allergenic response in nursery pigs. A total of 240 weaned pigs, 5.9 ± 0.8 kg body weight (BW), were allotted to 5 dietary treatments with 8 replicates in a randomized complete block design. Dietary treatments were low SBM diet (17% in phase 1 and 20% in phase 2), standard SBM diet (22% in phase 1 and 25% in phase 2), high SBM diet (30% in phase 1 and 35% in phase 2), high SBM diet with multienzyme supplementation (100 ppm of phytase, 500 ppm of carbohydrase, 250 ppm of protease, and 25 ppm of keratinase), and high SBM diet with multienzyme and acidifier (0.5%) supplementation. The enzyme combination was selected based on an in-vitro digestion experiment evaluating the degradation rate for antinutritional factors present in SBM. Diets were provided in a 3-phase feeding program: phase 1 (wk 1), phase 2 (wk 2 and 3), and phase 3 (wk 4 to 6); common corn-SBM based diet. Growth performance was measured at the end of each phase along with blood collection for serum IGF-1, IgA, and IgE analyses. One pig per pen was euthanized to collect intestinal tissue for histomorphology and ileal digesta for sIgA analysis. Data were analyzed using MIXED procedure of SAS and fecal score data was analyzed using FREQ procedure of SAS. Pigs fed high SBM diets with multienzyme or with multienzyme and acidifier tended to have a greater (P = 0.08) average daily gain compared with pigs fed High-SBM diet during phase 2 by 12.5%. Pigs fed high SBM diets with multienzyme and acidifier supplementation had greater (P &amp;lt; 0.05) average daily feed intake compared with pigs fed high SBM diet in phase 2. During phase 1 and 2, pigs fed high SBM diets supplemented with multienzyme or multienzyme plus acidifier had less incidence of soft and watery feces (χ2 &amp;lt; 0.05) than those fed high SBM diet. Dietary treatment did not affect serum concentrations of T4, IGF-1, IgA, and IgE. Pigs fed high SBM diet with multienzyme and acidifier supplementation had less (P &amp;lt; 0.05) concentration of sIgA in the ileal digesta on d 14 compared with high SBM diet (24.2 vs 33.8 mg/mL) but similar to low SBM diet (22.7 mg/mL). Pigs fed high SBM diet with multienzyme and acidifier supplementation had greater (P &amp;lt; 0.05) jejunal villus height to crypt depth ratio on d 7 compared with low SBM by 12%. In conclusion, supplementation with multienzyme or multienzyme and acidifier in high SBM diets could reduce the negative impact of high SBM inclusion in nursery pig diets by improving growth performance, promoting intestinal health, and reducing allergenic response in nursery pigs.

3 The impact of early-life cecal microbiota transplantation on injurious behaviors in egg-laying chickens

Abstract Injurious behaviors in laying hens are a critical issue facing the poultry egg industry. Recent studies have shown that the gut microbiota influences host brain function and behavioral characteristics in humans and various animals. In laying hens, it has been reported that injurious behaviors (such as aggression, feather pecking and cannibalism) are associated with dysregulation of the microbiota-gut-brain axis. To further interrogate the role of the microbiota-gut-brain axis on bird behavior, we used two inbred layer chicken lines, 63 and 72 (line 72 displays more aggressive behavior), and transferred pooled cecal contents from either line to day of hatch chicks. Cecal microbiota transplantation (CMT) from each line was conducted once daily, by gavage, from d 1 to 10, then boosted once weekly from wk 3 to 5. We hypothesized that the cecal microbiota composition and animal behavior in recipient birds would be similar to that of their donor animals because of the microbiota-gut-brain axis. Microbial communities were compared using 16S rRNA gene amplicon sequencing, behavior was recorded in home cages as well as in paired aggression tests, and physiological measurements were taken from brain tissue and blood to measure levels of neurotransmitters and immune function. When examining the differences in the donor birds, hypothalamic serotonin and tryptophan concentrations were greater for line 63 compared with line 72 birds (P &amp;lt; 0.05). Plasma corticosterone, heterophil/lymphocyte ratios, and central norepinephrine concentrations were less for line 63 birds (P &amp;lt; 0.05). Cecal microbial diversity was not statistically different between the lines, but many bacterial taxa were differentially abundant between the two lines. After cecal transplantation, 63-CMT (recipient) birds displayed less aggressive behavior with a greater hypothalamic serotonergic activity at wk 5, similar to donor birds. Correspondingly, two amplicon sequence variants (ASVs) belonging to Lachnospiraceae and one Ruminococcaceae UCG-005 ASV were positively correlated with the concentrations of brain tryptophan and serotonin, respectively. Along with greater aggression, 72-CMT birds seemed to have greater physical growth traits (increased body weight, ileal villus/crypt; P ≤ 0.05), but decreased concentrations of brain norepinephrine and dopamine, and the greatest ileal serotonin turnover in the ileum at wk 5 (P &amp;lt; 0.05). ASVs belonging to Mollicutes RF39 and GCA-900066225 in 72-CMT birds were negatively correlated with the brain 5-hydroxyindoleacetic acid (5-HIAA) at wk 5, and one Bacteroides ASV was negatively correlated with plasma serotonin at wk 16. Meanwhile, 63-CMT birds potentially had better gut health and immune function (greater concentrations of ileal mucosal secretory IgA, plasma IL-10; P &amp;lt; 0.05). These results suggest linkages among serotonergic activity, stress response, innate immunity, and gut microbiota populations and that CMT could be a novel strategy for reducing aggressive behavior through regulating signaling along the microbiota-gut-brain axis.

The use of bioluminescent enzyme bioassay for the analysis of human saliva: Advantages and disadvantages.

The purpose of the work was to find optimal conditions for bioluminescent enzymatic analysis of saliva (based on the use of NADH:FMN oxidoreductase + luciferase) and then to determine the biological effect of using bioluminescence assay of saliva to study the physiological state of the body under normal and pathological conditions. The saliva of snowboarders and students were studied in the "rest-training" model. The saliva of patients diagnosed with acute pharyngitis was examined in the "sick-healthy" model. Bioluminescence assay was performed with a lyophilized and immobilized bi-enzyme system using cuvette, plate, and portable luminometers. The concentrations of secretory immunoglobulin A (sIgA) and cortisol were determined by enzyme immunoassay, and the total protein content was measured by spectrophotometric method. The activity of the bioluminescent system enzymes increased as the amount and volume of saliva in the sample was decreased. The cuvette and plate luminometers were sensitive to changes in the luminescence intensity in saliva assay. Luminescence intensity correlated with the concentrations of sIgA and cortisol. The integrated bioluminescent index for saliva was reduced in the "rest-training" model and increased in the "sick-healthy" model. Thus, the non-invasive bioluminescent saliva analysis may be a promising tool for assessing the health of the population.

Profile of Schoolchildren with Different Nutritional Status After Social Isolation: Sedentary Behavior, Aerobic Fitness, Secretory Immunoglobulin-A, and Anxiety Symptoms

ABSTRACT Background The COVID-19 pandemic imposed social isolation measures, which impacted the child population, especially regarding physical and mental health aspects. Purpose The aim of this study was to investigate the relationship between sedentary behavior, cardiorespiratory fitness, immunoglobulin -A secretion, and anxiety symptoms in 267 children aged 6–11 years, eutrophic or overweight. Methods Sedentary behavior was evaluated by the time of exposure to screens. Cardiorespiratory fitness was assessed using the 6-minute run/walk test and immunoglobulin-A by salivary samples. Anxiety symptoms were reported by the SCAS-version for parents. Results It was observed that after a period of confinement, overweight in children had a negative impact on cardiorespiratory performance and sedentary behavior. Differences were also observed in the concentration and secretion rate of SIgA in the overweight group compared to the normal weight group. Discussion The return to face-to-face classes brought a high proportion of overweight students, who showed lower aerobic performance. Considering sedentary behavior, all groups presented values greater than 2 hours of daily exposure to screens. Translation to Health Education Practice Special attention is needed from health professionals and teachers in relation to reducing sedentary behavior and improving cardiorespiratory fitness in schoolchildren.

Exploration of skin redness and immunoglobulin A as markers of the affective states of hens

Non-invasive markers of affective states can help understanding animals' perception of situations and improving their welfare. These markers are scarce in avian species. In this study, we investigate the potential relation between alterations in facial skin redness in hens and their corresponding affective states. Six hens were filmed in both naturally unfolding scenarios and controlled tests designed to elicit various affective states. The facial skin redness was measured from images extracted from the videos. Our observations revealed that hens exhibited the highest degree of facial skin redness in negative situations of high arousal, a high redness in positive situations of high arousal, and the lowest in positive situations of low arousal. In a second study, we further examined whether facial skin redness and secretory immunoglobulin A (S-IgA) can serve as markers for the quality of the human-animal relationship. Two groups of hens, one habituated to humans (n=13) and one non-habituated (n=12), were compared for general fearfulness in an open field test and for fear of humans in a reactivity to human test. In the open-field test, there were no statistical differences in general fearfulness, facial skin redness or S-IgA concentrations between both groups. However, habituated hens exhibited significantly lower fearfulness and facial skin redness in the presence of humans compared to non-habituated hens in the reactivity to human test. Additionally, habituated hens showed significant lower S-IgA concentration in lachrymal fluid in the presence of humans, with no significant differences in saliva or cloacal samples. We propose that changes in facial skin redness reflect variations in affective states and can be used as a marker for assessing the quality of the human-hen relationship. The relationship between S-IgA concentrations and affective states requires further investigation.

The Influence of Black Tea on Secretory IgA from Saliva in Caries and Caries-Free Subjects

The crucial role of saliva in the prevention of dental caries is associated with the regulation of its flow rate as well as salivary protein. Black tea, derived from the plant Camellia sinensis, contains catechins and has been shown to have a beneficial effect on saliva in preventing tooth decay. Nevertheless, research on the oral health benefits of black tea is still limited. This study aimed to investigate the effects of black tea (Camellia sinensis) on salivary pH (potential hydrogen), salivary flow rate, lysozyme, and sIgA levels in caries and caries-free patients. The study used a quasi-experimental design with a pre-test and a post-test. Twenty six subjects (male or female) were selected and divided into two groups (caries and caries free), thirteen subjects for each. Saliva samples were obtained before and thirty minutes after tea consumption. The flow rate of saliva was measured by dividing the weight of saliva collected by the time. At the same time, secretory immunoglobulin A (sIgA) levels were analyzed by enzyme-linked immunosorbent assay (ELISA). The t-dependent and t-independent tests and Mann-Whitney tests were selected to evaluate the effect of drinking black tea on salivary flow rate and sIgA value. The results showed that black tea significantly increased salivary flow rate but did not significantly affect the concentration of sIgA. No statistically significant differences in salivary flow rate and sIgA were observed between patients with and without caries after tea consumption. Although the concentration of sIgA did not demonstrate a significant change, however, the salivary flow rate was significantly enhanced. Therefore, drinking black tea did not negatively affect the saliva in the oral cavity, suggesting it can be a good option for daily consumption due to its protective role against dental caries.

Clinical progression of parotid salivary gland sialadenosis based on the selected &lt;i&gt;H. pylori&lt;/i&gt; eradication therapy

Relevance. In approximately 50.82% of instances, sialadenosis of the parotid salivary gland (PSG) emerges as a sequel to gastrointestinal (GI) disorders. This condition is characterized by diminished saliva production (hyposalivation) and enlargement (hypertrophy) of the gland. In Russia, the prevalence of GI diseases linked to acid-related issues and H. pylori infection (HP) is notably high. The therapeutic approach to this pathology focuses on the eradication of HP, employing various treatment strategies that incorporate the use of a proton pump inhibitor (PPI). The efficacy of PPIs hinges on their ability to inhibit the proton pump, which is situated in the endothelial lining of the smooth muscle vessels, including those that encase the acinar cells of the PSG. An essential aspect of parotid secretion is its protective role, which is executed through the production of secretory immunoglobulin A (sIgA) within the gland's ductal system. Therefore, exploring the clinical progression of PSG sialadenosis under eradication therapy regimens holds substantial significance.Materials and methods. Between 2021 and 2023, 80 patients with parotid salivary gland (PSG) sialadenosis and Helicobacter pylori stomach infection were studied. They were divided into treatment groups: Group I (25 patients, aged 45.81 ± 6.39 years) received quadruple therapy (standard-dose PPI, amoxicillin 1,000 mg, clarithromycin 500 mg twice daily, and bismuthate tripotassium dicitrate 120 mg four times daily); Group II (55 patients, aged 47.79 ± 7.15 years) underwent standard triple therapy (double-dose PPI, amoxicillin 1,000 mg, and clarithromycin 500 mg twice daily). A control group consisted of 20 nearly healthy individuals (average age 26.46 ± 4.12 years). All subjects underwent comprehensive dental and PSG assessments, including questionnaires, sialometry, and measurement of (sIgA) levels in parotid secretion.Results. After eradication therapy, Group I patients treated with quadruple therapy exhibited a 1.2-fold reduction in PSG secretory function (p = 0.048) and a 1.2-fold decrease in sIgA concentration (p = 0.035) compared to initial levels. Group II patients receiving triple therapy demonstrated a significant 3.6-fold decline in PSG sialometry parameters (p = 0.002) and a 1.8-fold reduction in sIgA levels (p = 0.006) by the 14th day post-eradication, relative to pre-treatment figures. Conclusion. Therefore, patients with GI acid-related conditions linked to HP infection should be evaluated by a dentist for potential PSG sialadenosis. The presence of PSG sialadenosis in individuals with HP stomach infection warrants consideration in the selection and administration of eradication therapy.

DYNAMICS OF BIOCHEMICAL MARKERS OF HUMORAL IMMUNITY AND METABOLISM IN MANDIBULAR BONE TISSUE UNDER INFLUENCE ON REPARATIVE OSTEOGENESIS

The scientific and practical significance of our study lies in the application of various therapeutic measures aimed at accelerating the processes of reparative osteogenesis in the presence of perforated defects in the bone tissue of the mandible using hydroxyapatite-containing osteotropic material “Biomin GT bone graft» and thymaline (injections into the surrounding soft tissues) analyzed in our article.&#x0D; The aim of the study is to investigate the dynamics of secretory immunoglobulin A content and activity of acid and alkaline phosphatases in the oral fluid of rats under the conditions of influence on the processes of reparative osteogenesis when filling the bone defect with osteotropic material and injecting thymaline into the surrounding soft tissues at different study periods.&#x0D; Materials and methods. Experimental studies were conducted on 30 mature WAG population rats weighing 160-180 g, which were divided into five groups. The control group consisted of 6 intact rats, the first group included rats with a simulated hole defect of the mandible, the second group included rats with a simulated hole defect followed by closure of the bone defect with hydroxyapatite-containing osteotropic material, the third group included rats with a mandibular defect after thymaline injections into the surrounding soft tissues, and the fourth group included animals with a defect after filling the bone defect with osteotropic material and injections of thymaline into the surrounding soft tissues. The concentration of sIgA in the oral fluid of rats was determined by enzyme-linked immunosorbent assay using «The IgA Saliva ELISA kit» (Diametra, Italy). The activity of alkaline (ALP) and acid phosphatase (AP) in the biomaterial was measured using a set of reagents “Granum» Ukraine, Kharkiv. The optical density was measured using a STAT-FAX 303+ immunoenzyme analyzer.&#x0D; Results and discussion. A decrease in the content of sIgA in the oral fluid of rats of groups I-IV was found both on day 3 and day 7 of the study compared with the control group. On the 14th day, an increase (by 16.7%) in the content of sIgA in the oral fluid of rats of group IV was determined compared to the first group. The content of sIgA in the oral fluid of rats of groups I and III remained below the control by 29.6%, 32.7%, 29.9%, respectively. On the 28th day, only in rats of group IV, sIgA exceeded the value of its content in rats of the control group and was 52.6% higher compared to rats of group I. On the 3rd day, an increase in the activity of ALP from 102% to 111%, respectively, was observed in the oral fluid of rats of groups I-IV compared to intact animals. On the 7th day, the greatest (18.9%) decrease in the activity of ALP was observed in the oral fluid of rats of group IV; on the 14th day, only rats of group IV showed a decrease in the activity of ALP (by 38.1%) compared to the 3rd day. On the 28th day, a decrease in the activity of ALP (by 43.12%) was observed in the oral fluid of rats of group IV compared to day 3 and was equal to the control. On the third day, there was an increase in the activity of AP in the oral fluid of all rats. When comparing the values of AP activity in the oral fluid of rats of groups I and IV, a difference of 17.3% was determined. In rats of group IV on day 14: 25.6% decrease in AP activity compared to day 3 of the study, although it differed from the control group. However, statistically significant decrease in AP activity was observed in rats of group IV compared to groups I-III.&#x0D; Conclusions. In rats with a simulated mandibular perforation defect on the third and seventh days of observation, a deficiency of sIgA in the oral fluid was observed, indicating a decrease in the functioning of the humoral link of local immunity. In rats of group IV, on the 14th and 28th day of the study, the sIgA content was normalized. There is an activation of reparative osteogenesis in the bone tissue of the mandible in rats with a simulated hole defect under the conditions of its subsequent closure with hydroxyapatite-containing osteotropic material and injections of thymaline into the surrounding soft tissues. The determination of biochemical markers of bone metabolism, in particular alkaline and acid phosphatase in the oral fluid in mandibular fractures, can be used in clinical practice to improve the efficiency of diagnosing reparative osteogenesis in the jaw bones.