Ryegrass Lolium Research Articles

Occurrence of Li in groundwaters and plants from Dobrogea karst area, Romania

A positive association of Li consumption with the potentially protective and beneficial for the human health was reported (Barjasteh-Askari et al. 2020). Drinking water, grains, or vegetables can be a significant Li source for humans. Microdoses of Li intake may have antisuicidal, mood-stabilizing, antidepressive, and antimanic effects (Knudsen et al. 2017; Ng et al. 2019). The assessment of naturally occurring Li concentrations in water and food sources in different regions may present a high interest in the wellbeing of locals. In this study, a versatile quantitative ICP-MS method for Li quantitative determination in water and plant samples was optimized, and the relationship between Li, macroelements (Na, Mg, Al, K, Ca, Fe, Mn), and microelements (Cr, Co, Ni, Cu, Zn, Pb, Sr, Ba, V, As, Sr, Cd, Pb) concentration was assessed. Contents of Li, and micro- and macroelements were measured in groundwater (Praporgescu-GWR27, Closca-GWR28, Sipote-GWR29, and Tufani-GWR30) and plant samples (ryegrass—Lolium sp., nettles—Urtica sp., and mint—Mentha sp.) collected from Dobrogea karst area, Romania. The results indicated an acceptable precision in all studied matrixes and a reproducibility between 2.46 and 4.22% of the developed method. In the case of water, the highest Li concentration was measured in GWR27 followed by GWR28 (12.2 and 5.6 µg/L), while in the case of the plant’s samples, Lolium sp. collected from GWR28 and GWR27 (11.1 and 8.8 mg/kg DW) had the highest Li concentration.

Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms.

Perennial ryegrass (Lolium perenne L.) is an important turfgrass and gramineous forage widely grown in temperate regions around the world. However, its perennial nature leads to the inevitable exposure of perennial ryegrass to various environmental stresses on a seasonal basis and from year to year. Like other plants, perennial ryegrass has evolved sophisticated mechanisms to make appropriate adjustments in growth and development in order to adapt to the stress environment at both the physiological and molecular levels. A thorough understanding of the mechanisms of perennial ryegrass response to abiotic stresses is crucial for obtaining superior stress-tolerant varieties through molecular breeding. Over the past decades, studies of perennial ryegrass at the molecular and genetic levels have revealed a lot of useful information to understand the mechanisms of perennial ryegrass adaptation to an adverse environment. Unfortunately, molecular mechanisms by which perennial ryegrass adapts to abiotic stresses have not been reviewed thus far. In this review, we summarize the recent works on the genetic and molecular mechanisms of perennial ryegrass response to the major abiotic stresses (i.e., drought, salinity, and extreme temperatures) and discuss new directions for future studies. Such knowledge will provide valuable information for molecular breeding in perennial ryegrass to improve stress resistance and promote the sustainability of agriculture and the environment.

Analytical purity of old New Zealand forage seed samples and detection of fungal and insect contaminants including ryegrass endophyte and Argentine stem weevil

ABSTRACT Pasture seed samples that were harvested around 1917 in New Zealand were analysed for purity, Epichloë endophyte and insects. Samples consisted of 13 packets of seed varying from 2.2 to 15.0 g and comprising, Chewings fescue (Festuca rubra L.), cocksfoot (Dactylis glomerata L.), oat (Avena sativa L.), perennial ryegrass (Lolium perenne L.) and red clover (Trifolium pratense L.). These findings show that in the period prior to modern seed testing laboratories only four of the 13 samples (31%) could possibly have met today’s seed certification standards as it relates to seed purity. When compared with published purities and weed content of seed of the era these samples appear typical. Endophyte testing on the four ryegrass seed samples and the ryegrass contaminants found in the Chewings fescue and cocksfoot samples showed sample-to-sample variation in endophyte infection from nil to 91%. One cocksfoot sample, most likely harvested from the Banks Peninsula, contained an Argentine stem weevil. Subsequent checking of entomological collection records also revealed specimens being present in the Wellington region from 1916. Together these occurrences precede the earliest published record for the species in New Zealand by ten years and suggest that it may have been widespread at that time.

Hot Foam and Nitrogen Application to Promote Spring Transition of “Diamond” Zoysiagrass (Zoysia matrella (L.) Merr.) Overseeded with Perennial Ryegrass (Lolium perenne L.)

In transition areas, cool season turfgrasses are overseeded in autumn to maintain the high quality of dormant warm season turfgrasses, while in spring several agronomic methods (scalping, coring, topdressing, verticutting, irrigation, and targeted fertilization) or chemical desiccation are adopted to remove the cool season turfgrasses from the stand. To reduce chemical applications, several methods of “thermal weeding” have been experimented with, but little is known about these methods in zoysiagrass (Zoysia spp. Willd) spring transition. A study was conducted at the University of Pisa, Italy, on Manila grass (Zoysia matrella (L.) Merr., cv “Diamond”) (Zm) overseeded with perennial ryegrass (Lolium perenne L.) (Lp) with the aim of comparing different methods of cool season grass suppression (scalping and hot foam) and different application rates of nitrogen. To assess treatment effect, green cover, turf quality, turf color, shoot density, and some vegetation indices (GLI, DGCI and NDVI) were determined. An average green cover of at least 90% was obtained on all plots seven weeks after the treatments. While scalping had minor effects on turf appearance and on polystand composition, hot foam had a stronger effect on turf color, green cover, and turf quality in the weeks following application. Once it had recovered from the hot foam treatments, the turf had a greater number of Zm shoots and a relevant reduction of Lp shoots. The hot foam was very effective in suppressing Lp while maintaining Zm recovery capacity.

Flux of Root-Derived Carbon into the Nematode Micro-Food Web: A Comparison of Grassland and Agroforest

Carbon (C) cycling is crucial to agroecosystem functioning. Important determinants for the belowground C flow are soil food webs, with microorganisms and microfaunal grazers, i.e., nematodes, as key biota. The present study investigates the incorporation of plant-derived C into the nematode micro-food web under two different cropping systems, grassland (ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.)) and agroforest (willow (Salix schwerinii Wolf and Salix viminalis L)). To quantify the C flux from the plant into the soil micro-food web, grass and willow were pulse-labeled with 13CO2 and the incorporation of 13C into the nematode trophic groups was monitored 3, 7, 14 and 28 days after labeling. The natural stable isotope signals (13C/12C, 15N/14N) were analyzed to determine the structure of the nematode micro-food web. The natural isotopic δ15N signal revealed different trophic levels for omnivores and predators in grassland and agroforest soils. The incorporation of plant C into nematode tissue was detectable three days after 13CO2 labeling with the highest and fastest C allocation in plant feeders in grassland, and in fungal feeders in agroforest soil. C flux dynamics between the aboveground vegetation and belowground micro-food web varied with cropping system. This demonstrates that crop-specific translocation of C affects the multitrophic interactions in the root environment, which in turn can alter soil nutrient cycling.

Nature-Based Solutions for the Sustainable Management of Urban Soils and Quality of Life Improvements

The rehabilitation and restoration of land-based ecosystems is a key strategy for recovering the services (goods and resources) ecosystems offer to humankind. The use of nature-based solutions (NBSs) to restore degraded soil functions and improve soil quality can be a sustainable and successful strategy to enhance their ecosystem services by working together with the forces of nature and using well-designed measures that require less maintenance, are more cost-effective, and if constructed in the right way may even be more effective over long periods because nature’s forces can increase the structural efficiency. In this study, we aimed to (i) evaluate the bioremediation capacity of some grasses and their suitability for lawn planting in settlements (in residential and non-residential areas, along roads, etc.) and (ii) propose technological solutions for their practical application in an urban environment. Emphasis was placed on the potential of some perennial grasses and their application for the bioremediation of polluted urban soils, including perennial ryegrass (Lolium perenne L.), crested wheatgrass (Agropyron cristatum L.), tall fescue (Festuca arundinacea Schreb), and bird’s foot trefoil (Lotus corniculatus L.). A case study from the city of Plovdiv (Bulgaria) is presented, together with an effective technological solution for the establishment of urban lawns and the roadside green buffer patches.

Effects of Cultivar, Nitrogen Rate and Biostimulant Application on the Chemical Composition of Perennial Ryegrass (Lolium perenne L.) Biomass

The aim of this study was to determine the effects of cultivar, nitrogen (N) rate, and biostimulant application on the chemical composition of the aboveground biomass of perennial ryegrass (Lolium perenne L.). A small-area field experiment was established in the Agricultural Experiment Station in Tomaszkowo (53°42′40.8″ N 20°26′04.7″ E, north-eastern Poland). The experiment had a split-plot design with three replications, and the experimental variables were as follows: (i) perennial ryegrass cultivar: Bajka and Baronka, (ii) N fertilizer rate: 0, 120, 240 kg N ha−1, (iii) application of biostimulants: Blatt Boden-Foliar (BB-F) and Blatt Boden-Foliar + Blatt Boden-Multical (BB-F + BB-M). This study demonstrated that the tetraploid cultivar Baronka had a more desirable chemical composition than the diploid cultivar Bajka. The biomass of cv. Baronka had a higher content of CP, CF, and K, and it was characterized by higher leaf greenness (SPAD) values. Nitrogen fertilization considerably increased the content of CP, P, and K, and leaf greenness (SPAD) values in both cultivars, and the noted increase was higher when N was applied at 240 kg ha−1. The N fertilizer rate of 120 kg ha−1 led to a significant decrease in the average Ca content of plants, whereas the N fertilizer rate of 240 kg ha−1 had no significant effect on Ca concentration. The tested biostimulants significantly affected the chemical composition of perennial ryegrass biomass, and their influence was greater when they were applied in combination. The foliar application of Blatt Boden-Foliar and Blatt Boden-Multical increased the content of CP, P, and chlorophyll in perennial ryegrass leaves, whereas it decreased the accumulation of CF, K, and Ca in plants. The analyzed biostimulants had a positive effect on the chemical composition of perennial ryegrass biomass. This is an important practical consideration because high-quality green fodder for livestock can be produced while minimizing the use of mineral fertilizers and adverse environmental impacts.

Transcriptome profiling revealed candidate genes, pathways and transcription factors related to nitrogen utilization and excessive nitrogen stress in perennial ryegrass

Ryegrass (Lolium perenne L.), a high-quality forage grass, is a good nutrient source for herbivorous livestock. However, improving nitrogen use efficiency and avoiding nitrate toxicity caused by excessive nitrogen are continual challenges in ryegrass production. The molecular mechanism underlying the response of ryegrass to nitrogen, especially excessive nitrogen, remains unclear. In this study, the transcriptomic changes under different nitrogen levels were investigated in perennial ryegrass by high-throughput next-generation RNA sequencing. Phenotypic characterization showed that treatment with half of the standard N concentration (N0.5) led to a better growth state than the other three treatments. The treatments with the standard N concentration (N1) and treatments with ten times higher than the standard N concentration (N10) contained excessive nitrogen, which placed stress on plant growth. Analysis of differentially expressed genes indicated that 345 and 104 genes are involved in the regulation of nitrogen utilization and excessive nitrogen stress, respectively. KEGG enrichment analysis suggested that “photosynthesis-antenna proteins” may respond positively to appropriate nitrogen conditions, whereas “steroid biosynthesis”, “carotenoid biosynthesis” and “C5-branched dibasic acid metabolism” were identified as the top significantly enriched pathways in response to excessive nitrogen. Additionally, 21 transcription factors (TFs) related to nitrogen utilization were classified into 10 families, especially the AP2-EREBP and MYB TF families. Four TFs related to excessive nitrogen stress were identified, including LOBs, NACs, AP2-EREBPs and HBs. The expression patterns of these selected genes were also analyzed. These results provide new insight into the regulatory mechanism of ryegrass in response to nitrogen utilization and excessive nitrogen stress.

Phylogenetic Analysis of Ryegrass (Lolium rigidum) Populations and the Proliferation of ALS Resistance in Saudi Arabia

Morphological and simple sequence repeat (SSR) approaches were used to determine the genetic diversity of 29 ryegrass (Lolium rigidum) genotypes belonging to eight populations collected from several regions in Saudi Arabia. In this study, 50 in Silico-developed SSR markers derived from genomic and expressed sequence tag (EST) microsatellites were examined. Analysis of variance showed highly significant differences in all studied traits. Cluster analysis based on the morphological data of the 29 Lolium genotypes and using PAST (paleontological statistics) software was performed. According to the results, clustering was based mostly on genotype location. The sensitive genotypes for herbicide were clustered in one group. In addition, using EST-SSR markers, we observed the existence of a considerable number of genetic variations among Lolium genotypes. From these markers, only 31 produced reasonable amplification products. The results showed that 23 SSR markers revealed that 74.19% were polymorphic. The number of alleles detected per primer ranged from one to five in the primer LTC SSR1. The tested primers amplified 1434 bands across eight populations, with an average of 46.26 bands per primer. The polymorphism information content (PIC) values ranged from 0.11 to 0.76 for the primers LT EST-SSR5 and LTC SSR1. The unweighted pair group method with arithmetic average (UPGMA) clustering of the 29 genotypes representing eight populations was based essentially on their locations and herbicide-tolerance levels. Most of the populations formed into four clusters, together representing genotypes. Moreover, the tolerant populations were distinguished from the sensitive ones. The relationship between the genetic diversity and geographical source of Lolium rigidum populations of Saudi Arabia was revealed through this study. The results showed that the efficiency of developed SSR markers are transferable across species. They have been helpful to assess the genetic diversity of the ryegrass population as this could be applied to differentiate between tolerant and sensitive populations of ryegrass.

Fertilizer Efficiency and Risk Assessment of the Utilization of AOD Slag as a Mineral Fertilizer for Alfalfa (Medicago sativa L.) and Perennial Ryegrass (Lolium perenne L.) Planting

Argon oxygen decarburization (AOD) slag is the by-product of the stainless steel refining process, which has caused considerable environmental stress. In this work, the utilization of AOD slag as mineral fertilizer for alfalfa (Medicago sativa L.) and perennial ryegrass (Lolium perenne L.) planting were investigated by pot experiments. The morpho-physiological parameters of biomass, plant height, root morphology and the biochemical parameters of malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, catalase (CAT) activity, peroxidase (POD) activity, and chlorophyll were measured. The accumulation of chromium in plants was also determined for an environmental safety perspective. It was found that low rates (≤0.5 wt.% for alfalfa and ≤2 wt.% for perennial ryegrass) of AOD slag fertilization are beneficial to the growth of these two plants. However, the soil enrichment with higher AOD slag amounts resulted in the reduction of biomass, plant height, and root growth. Compared with the alfalfa, the perennial ryegrass showed higher tolerance for AOD slag fertilization. The toxicity of the utilization of AOD slag as mineral fertilizer for perennial ryegrass planting is slight. Health risks induced by the consumption of the alfalfa grown on the soil with high AOD slag rates (≥8 wt.%) were detected.

Animal safety of a tall fescue endophyte (Epichloë sp.) in a perennial ryegrass (Lolium perenne) host

ABSTRACT Aims To assess animal health and production in sheep grazing perennial ryegrass (Lolium perenne) infected with a tall fescue endophyte (Epichloë sp.). Methods Three replicates of pure perennial ryegrass pastures infected with AR501 tall fescue endophyte (AR501 HE), AR1, AR37 or standard endophyte (STD) (all >85% infection) and a low endophyte control (AR501 LE) were grazed by 10 lambs for 7 weeks during late summer/early autumn, 2017. AR501 HE and AR501 LE were in the same tetraploid cultivar and the remaining treatments were in the same diploid cultivar. Lambs were weighed on Days 0, 19, 37 and 48 and assessed for ryegrass staggers on a 0–4 tremor scale nine times from Day 13. On Days 14 and 20, rectal temperatures and respiration rates were measured in lambs grazing AR501 HE, AR501 LE and STD under heat load. Pasture samples from each treatment were taken regularly (8 times) for analysis of known endophyte secondary metabolites. Results Peak mean ryegrass staggers scores for lambs grazing STD and AR37 treatments were 3.9 and 2.7, respectively, indicating environmental conditions were conducive to toxicity. Lambs grazing AR501 HE showed no ryegrass staggers at any date. The liveweight of STD lambs was up to 6.6 kg less than some or all other treatments on Days 19, 34 and 48, but there was no evidence of a difference between the liveweight of AR501 HE lambs and AR501 LE, AR37 or AR1 lambs on any date. Rectal temperatures and respiration rates of STD lambs were higher than AR501 HE lambs by 0.2°C and 26 breaths/minute, respectively. Apart from lower rectal temperature of AR501 HE lambs on Day 14, there was no evidence of differences between the AR501 HE and AR501 LE lambs. N-Formylloline was the only loline present in AR501 HE herbage (350–860 mg/kg). Peramine in AR501 HE herbage (42–77 mg/kg) was greater than that in STD and AR1 herbage on five and seven of the eight sampling dates, respectively. Conclusion Lambs grazing AR501 HE showed no ryegrass staggers, exacerbated heat stress or suppressed liveweight gain compared with lambs grazing AR501 LE in a season when endophyte-induced toxicity was evident in STD and AR37 pastures. This suggests AR501 tall fescue endophyte does not produce toxic concentrations of secondary metabolites. Clinical relevance This study suggests that there are no secondary metabolites produced by the tall fescue AR501 endophyte-perennial ryegrass association which affect animal health or production.

Agri-environment schemes are associated with greater terrestrial invertebrate abundance and richness in upland grasslands

Agri-environment schemes are a key mechanism by which agricultural sustainability is encouraged by subsidising farmers to adopt environmentally friendly management (e.g. reduction of inputs) to maintain and enhance the delivery of biodiversity-associated ecosystem services. Studies testing the efficacy of agri-environment schemes have yielded varying results, and few have focused on upland (marginal or Less Favoured Area) grassland (> 150 m above sea level) where productivity is poor. This study used a factorial field experiment to examine patterns in plant communities and terrestrial invertebrates between agri-environment scheme and conventionally managed semi-improved and improved upland grasslands, using 90 spatially paired fields. Total plant species richness and rare plant species richness (those with < 10% occurrence) were unaffected by agri-environment scheme management, but were significantly higher on semi-improved than improved grasslands. Total and rare invertebrate abundance and family-level richness were unrelated to grassland type (semi-improved or improved). Total and rare invertebrate abundances were 4% and 218% higher, and total and rare invertebrate family-level richness were 17% and 14% higher in agri-environment scheme than conventionally managed fields, respectively. Here, we show that agri-environment scheme management of marginal or Less Favoured Area upland grassland was associated with higher multi-taxa invertebrate abundance and richness associated with swards indicative of wetter conditions with lower dominance of perennial ryegrass (Lolium perenne) and greater coverage of other native grass species compared to conventional management. This suggests that agri-environment schemes may maintain, enhance or offset declines in terrestrial invertebrates and their associated ecosystem service delivery by maintaining more diverse swards, and suggests that they make a positive contribution to biodiversity conservation.

Soil and Plant Responses to Phosphorus Inputs from Different Phytase-Associated Animal Diets

The over-supplementation of animal feeds with phosphorus (P) within livestock-production systems leads to high rates of P excretion and thus to high P loads and losses, which negatively impact the natural environment. The addition of phytase to pig and poultry diets can contribute to reducing P excretion; however, cascading effects of phytase on plant–soil systems remain poorly understood. Here, we addressed how three different diets containing various levels of exogenous phytase, i.e., (1) no-phytase, (2) phytase (250 FTU), and (3) superdose phytase (500 FTU) for pigs (Sus scrofa domesticus) and broilers (Gallus gallus domesticus) might affect P dynamics in two different plant–soil systems including comfrey (Symphytum × uplandicum) and ryegrass (Lolium perenne). We found that differences in phytase supplementation significantly influenced total P content (%) of broiler litter and also pig slurry (although not significantly) as a result of dietary P content. P Use Efficiency (PUE) of comfrey and ryegrass plants was significantly higher under the intermediate ‘phytase’ dose (i.e., commercial dose of 250 FTU) when compared to ‘no-phytase’ and ‘superdose phytase’ associated with pig slurry additions. Soil P availability (i.e., water soluble P, WSP) in both comfrey and ryegrass mesocosms significantly decreased under the intermediate ‘phytase’ treatment following pig slurry additions. Dietary P content effects on P losses from soils (i.e., P leaching) were variable and driven by the type of organic amendment. Our study shows how commercial phytase levels together with higher dietary P contents in pig diets contributed to increase PUE and decrease WSP thus making the plant–soil system more P conservative (i.e., lower risks of P losses). Our evidence is that dietary effects on plant–soil P dynamics are driven by the availability of P forms (for plant uptake) in animal excretes and the type of organic amendment (pig vs. broiler) rather than plant species identity (comfrey vs. ryegrass).

Genome-Wide Association Mapping of Crown and Brown Rust Resistance in Perennial Ryegrass.

A population of 239 perennial ryegrass (Lolium perenne L.) genotypes was analyzed to identify marker-trait associations for crown rust (Puccinia coronata f. sp. lolii) and brown rust (Puccinia graminis f. sp. loliina) resistance. Phenotypic data from field trials showed a low correlation (r = 0.17) between the two traits. Genotypes were resequenced, and a total of 14,538,978 SNPs were used to analyze population structure, linkage disequilibrium (LD), and for genome-wide association study. The SNP heritability (h2SNP) was 0.4 and 0.8 for crown and brown rust resistance, respectively. The high-density SNP dataset allowed us to estimate LD decay with the highest possible precision to date for perennial ryegrass. Results showed a low LD extension with a rapid decay of r2 value below 0.2 after 520 bp on average. Additionally, QTL regions for both traits were detected, as well as candidate genes by applying Genome Complex Trait Analysis and Multi-marker Analysis of GenoMic Annotation. Moreover, two significant genes, LpPc6 and LpPl6, were identified for crown and brown rust resistance, respectively, when SNPs were aggregated to the gene level. The two candidate genes encode proteins with phosphatase activity, which putatively can be induced by the host to perceive, amplify and transfer signals to downstream components, thus activating a plant defense response.

Four In Silico Designed and Validated qPCR Assays to Detect and Discriminate Tilletia&nbsp;indica and T. walkeri, Individually or as a Complex.

Simple SummaryPlant pathogens represent a constant threat to human and animal food, as well as the economy. International trading is constantly expanding and has been known as a means of transportation and introduction for plant pests (e.g., bacteria, viruses, fungi, and insects) in new areas. They can damage or completely ruin a harvest and there are often strict regulations for the most unwanted plant pests in order to keep their incidence confined. The fungal plant pathogen Tilletia indica causes Karnal bunt, a wheat disease that breaks or hollows grains, grows in dark powdery masses, and emits a foul fishy odor, and is therefore highly regulated by a number of country authorities, many of which respond by imposing quarantine regulations. While there are many diagnostic methods developed (microscopy, molecular assays, etc.) to identify Karnal bunt, they have limitations. This study presents four highly sensitive quantitative PCR assays with molecular probes targeting unknown genomic regions for the detection and identification of T. indica and T. walkeri—its closest relative—and the species-complex including both species. Bioinformatics analyses of DNA sequences were used to design the toolkit presented.Several fungi classified in the genus Tilletia are well-known to infect grass species including wheat (Triticum). Tilletia indica is a highly unwanted wheat pathogen causing Karnal bunt, subject to quarantine regulations in many countries. Historically, suspected Karnal bunt infections were identified by morphology, a labour-intensive process to rule out other tuberculate-spored species that may be found as contaminants in grain shipments, and the closely-related pathogen T. walkeri on ryegrass (Lolium). Molecular biology advances have brought numerous detection tools to discriminate Tilletia congeners (PCR, qPCR, etc.). While those tests may help to identify T. indica more rapidly, they share weaknesses of targeting insufficiently variable markers or lacking sensitivity in a zero-tolerance context. A recent approach used comparative genomics to identify unique regions within target species, and qPCR assays were designed in silico. This study validated four qPCR tests based on single-copy genomic regions and with highly sensitive limits of detection (~200 fg), two to detect T. indica and T. walkeri separately, and two newly designed, targeting both species as a complex. The assays were challenged with reference DNA of the targets, their close relatives, other crop pathogens, the wheat host, and environmental specimens, ensuring a high level of specificity for accurate discrimination.

Prior Forage Type Influences Ruminal Responses to a Wheat Grain Challenge in Lactating Dairy Cows.

Simple SummaryHigh-producing dairy cows require more than just pasture to meet the energy demands of milk production. Wheat is an excellent energy source for milk production; however, cows require careful adaptation and monitoring to avoid ruminal upset when large amounts of wheat are introduced. The results of this study show that careful selection of the forage that precedes wheat could allow safer and more aggressive grain introduction strategies to be used in the dairy industry.To increase the dry matter and metabolisable energy intake of cows, dairy farmers often supplement pasture with concentrates and conserved fodder. Feeding large amounts of highly fermentable concentrates to cows can result in metabolic issues, such as ruminal acidosis, and thus safer but more efficient introduction strategies are desirable. We assessed the role that forages play in ruminal, behavioural and production responses to a wheat grain challenge in dairy cows with no previous wheat adaptation. Multiparous lactating Holstein dairy cows (n = 16) were fed a forage-only diet of either lucerne (Medicago sativa) hay, perennial ryegrass (Lolium perenne L.) hay or one of two cultivars of zero-grazing fresh perennial ryegrass herbage (Bealey or Base), for 3 weeks. The forage diet was then supplemented with crushed wheat grain at 8 kg dry matter/cow day−1, with no adaptation period. Wheat comprised between 32 and 43% of total dry matter intake. Cows fed hay maintained a higher mean ruminal fluid pH than those fed herbage, on both the forage-only diet (6.43 vs. 6.17) and the forage plus wheat diet (6.03 vs. 5.58). Following supplementation of wheat, cows fed herbage exhibited minimum ruminal fluid pH levels indicative of acute ruminal acidosis, at 5.15 and 5.06 for cultivars Bealey and Base, respectively. Furthermore, for both herbage cultivars, adding wheat resulted in a ruminal fluid pH under 6 for >20 h/day. The ruminal environment of cows fed lucerne hay remained most stable throughout the grain challenge, spending the least amount of time below pH 6.0 (9.0 h/day). Hay created a ruminal environment that was better able to cope with the accumulation of acid as wheat was digested. A combination of increased ruminating time and a slower rate of fermentation, due to higher neutral detergent fiber and lower metabolisable energy concentrations in the hays, is likely responsible for the higher ruminal fluid pH values. Forage plays a critical role in wheat introduction strategies; aggressive adaptation strategies could be implemented when a hay such as lucerne is used as the base forage.

Estimation of forage consumption for two dairy cattle biotypes in the high Andean tropics of Nariño, Colombia

The implementation of efficient grazing technologies in high mountain cattle ranches in the department of Nariño (Colombia) increases the sustainability of these production systems and promotes the conservation of the landscape design. With the objective of evaluating the dry matter intake of F1 (Kiwi-cross x Holstein) and Holstein cattle biotypes, present in hillside areas of the Nariño mountain, a preliminary trial of individual consumption of ryegrass (Lolium perenne) was carried out in Pasto (Nariño-Colombia) with the gauging technique, and the results were compared with models reported in the literature. The data were analyzed as a completely randomized design, with repeated measures over time. Intake per gauging was higher (p&lt;0.05) in Holstein cows, with respect to F1 cows (12.74 vs. 9.69 kg/dry-matter/day), but per unit live weight was similar (p&gt;0.05). On the other hand, the milk composition of F1 cows was superior (p&lt;0.05) for fat, protein, and total solids (%). In conclusion, the two breeds evaluated recorded the same forage intake capacity as a function of live weight, but, due to the larger size of the Holstein cows the absolute intake was higher, indicating that an adjustment in forage supply should be made according to the size of the animals. This work contributes to improve grazing techniques, making the use of pastures and their environment more sustainable, which favors productive efficiency and improves the quality of life for the producer families in the area.

Evaluation of grass mixtures of a lawn type in the conditions of forest-steppe of the Middle Volga region

In 2019-2020, the quality of lawn-type grass mixtures based on creeping clover (Trifolium repens L.), meadow fescue (Festuca pratensis), pasture ryegrass (Lolium perenne) and meadow bluegrass (Poa pratensis) was evaluated in the conditions of the forest-steppe of the Middle Volga region (Penza region). The studied factors are the composition of the mixtures (creeping clover + bluegrass component), the seeding rates of the components (40 + 70 %; 55 + 55 %; 70 + 40 %) and the background of mineral nutrition (without fertilizers, P45K45, N30P45K45). The growing season during the years of research was characterized by arid conditions, the hydrothermal coefficient (HTC) for the period May-September in 2019 was 0.79, in 2020 - 0.76. On average, over two years of grass mixtures of creeping clover with meadow bluegrass at a seeding rate of 40 + 70 % and 70 + 40 % (from the seeding rate in pure form), creeping clover with meadow fescue with a seeding rate of 70 + 40 % components at all doses of mineral fertilizers had the highest comprehensive assessment of the quality of the lawn. The grass mixtures of creeping clover with meadow bluegrass (70 + 40 %) and creeping clover with meadow fescue (70 + 40 %) against the background of application of N30P45K45 had the maximum indicators in terms of herbage density (1425 and 1475 pcs/m2), which significantly exceeded the control and the variant with the introduction of Р45К45. In these variants, the highest indicators of projective cover (97.5 %) and a comprehensive assessment of "excellent" and "highest quality". According to weediness, the crops were estimated at 1 point (slightly weedy), per 1 m2 of annual and biennial weeds there were 24-27 pcs., rhizome weeds - less than 10 pcs. per 1 m2, the area of weed projective cover did not exceed 5 %.

Response of Cover Crops to Phytopythium vexans, Phytophthora nicotianae, and Rhizoctonia solani, Major Soilborne Pathogens of Woody Ornamentals

Management of plant diseases is a subject of concern for researchers as well as growers. Different management practices are being developed and used to combat the rising number of plant pathogens, which threaten nursery crop production. Use of cover crops for sustainable management of soilborne diseases is being explored as an alternative strategy to the chemicals. However, the potential threat of these cover crops acting as a secondary host of these devastating soilborne pathogens has not been described. We studied the response of the major cover crops being used by woody ornamental growers in the Southeastern United States to Phytopythium vexans, Phytophthora nicotianae, and Rhizoctonia solani in greenhouse conditions to identify the effective cover crops that can be used in a nursery field production system. Data related to post-emergence damping-off and plant growth parameters (plant height increase and fresh weight) were recorded. Similarly, cover crop roots were assessed for root rot disease severity using a scale of 0–100% roots affected. Among the tested cover crops, the grass cover crops triticale (×Triticosecale Wittm. ex A. Camus.), annual ryegrass (Lolium multiflorum L.), Japanese millet (Echinochloa esculenta (A. Braun) H. Scholz), and the legumes Austrian winter pea (Pisum sativum var. arvense (L.) Poir) and cowpea ‘Iron and Clay’ (Vigna unguiculata (L.) Walp.), showed lower root rot disease severity and post-emergence damping-off in the soil inoculated with P. nicotianae, R. solani, or P. vexans compared to the other crops. Since these cover crops can act as non-host crops and benefit the main crop in one way or another, they can be used in the production system. Further research is recommended to evaluate their performance in a natural field setting.

Grass Growth and N2O Emissions From Soil After Application of Jellyfish in Coastal Areas

The supply of nutrients for agricultural production faces enormous challenges as food security and sustainability goals have to be ensured. Processing of marine biomass has high potential to provide nutrients for agricultural purposes in coastal areas. One underexplored resource are jellyfish, which occur as blooms and by-catch of the fishing industry. In this context, a pot experiment investigated the effects of jellyfish as a fertilizer on biomass accumulation of annual ryegrass (Lolium multiflorum), and its effect on the important greenhouse gas N2O as a sustainability indicator of novel fertilizers. Dried and ground jellyfish was applied [3 species: Aurelia aurita (AA), Cyanea capillata (CC), Periphylla periphylla (PP)] and compared with an unfertilized and a mineral fertilized (calcium-ammonium-nitrate, CAN) treatment. Dried jellyfish and CAN were applied at equal N rates of 5 g N per m2. The N2O-fluxes from soil were measured over 56 days after fertilizer application. Grass dry matter yields, when using CC and PP treatments, were not significantly different to the CAN treatment (p &amp;gt; 0.05). After reducing its salinity, AA also showed no differences to CAN on plant growth and the lowest coefficient of variation for dry matter yield as an indicator for yield stability. Accumulated N2O-emissions were lowest in the control and were 3-times higher in AA and CC compared to CAN (p &amp;lt; 0.05). If salinity levels are moderate, jellyfish application to soil can compete with artificial mineral fertilizers in terms of N-supply for above- and belowground yield response, regardless of jellyfish species used. However, elevated N2O-emissions are likely to affect its suitability for large-scale application. Nevertheless, if energy-efficient methods of drying and desalination of jellyfish can be developed, in coastal areas dried jellyfish is a valuable fertilizer in coastal areas, particularly in situations where nutrient supplies for agriculture are limited.