Determination Of Rutin Research Articles

A Novel, Selective, and Fast Electrochemical Sensor Based on an 8-Nitroquinoline-Coated Pencil Graphite Electrode for Rutin Determination in Orange Juice

In this study, we developed a facile and low-cost method to prepare a modified electrode by coating the pencil graphite electrode (PGE) surface with 8-nitroquinoline (8-NQ). The surface modification process was carried out by electrochemical reduction of 8-NQ on the PGE surface in ethanol solution. The optimum conditions were determined for the experimental studies. The differential pulse voltammetry was used to determine rutin with the modified electrode (8-NQ/PGE). The developed electrode showed excellent performance for rutin oxidation in a pH 3.0 Britton Robinson buffer (BR). In this media, linearly increasing anodic peak currents were observed with the concentration of rutin in two wide concentration ranges (i.e., 0.016–0.96 μM and 0.96‒19.73 μM), the sensor showed a low detection limit (i.e., 4.14 nM) (3 s m−1). It displayed good stability and selectivity. Also, it was used successfully for the determination of rutin in orange juice samples.

Advanced stability-indicating gradient elution reverse phase-high performance liquid chromatography method development and validation for simultaneous estimation of rutin and curcumin using an analytical quality by design approach

This research delves into a systematic Analytical Quality by Design (AQbD) approach to develop and validate a stability-indicating robust RP-HPLC method for the simultaneous determination of Rutin and Curcumin, compounds known for their anti-inflammatory, antioxidant, and wound-healing properties. Utilizing QbD principles, critical method parameters (CMPs) were identified and screened using design of experiments (DoE) and optimized via response surface methodology (RSM) with Central Composite Design (CCD). The optimized chromatographic separation used a Phenomenex Luna C18 column with gradient elution, using methanol and 0.1% formic acid, a flow rate of 1 ml/min, and detection at 345 nm (isosbestic point). The retention times were 5.63 mins for Rutin and 17.01 mins for Curcumin. The stability indication of the developed HPLC method was performed, and validation parameters were meticulously conducted following the ICH Q2(R1) guidelines, demonstrating linearity over 2-10 μg/ml for Rutin and 5-25 μg/ml for Curcumin, with correlation coefficients of 0.9999 and 0.9998, respectively. Precision studies showed %RSD within acceptable limits, and accuracies ranged between 97-100%. This validated HPLC method offers a reliable, sensitive approach for simultaneous quantifying Rutin and Curcumin, suitable for routine analysis in pharmaceutical laboratories and quality control.

Ratiometric Electrochemical Sensor Using Chitosan@Rhodamine B/Gold Nanoparticles (AuNPs) for the Sensitive Determination of Rutin by Differential Pulse Voltammetry (DPV)

Internal reference probes can effectively eliminate internal and external interferences. Hence, finding a suitable internal reference probe is the key to ratiometric electrochemistry. In this study, a simple and sensitive ratiometric electrochemical sensor for the determination of rutin was prepared for the first time using rhodamine B (Rh B) as an internal reference probe. The modified electrochemical sensor was constructed by electrodepositing gold nanoparticles (AuNPs) on a glassy carbon electrode followed by drop-coating chitosan-immobilized rhodamine B (CS@Rh B). Rh B has a dual function in that it has a stable electrochemical signal (1.18 V) (vs. Ag/AgCl) that is completely separated from the oxidation peak of rutin (0.52 V) to serve as an internal reference probe. At the same time, it synergistically enhances the electrochemical signal of rutin with AuNPs. When differential pulse voltammetry was used for the determination of rutin, the current ratio of I Rutin/I Rh B was employed as the signal for rutin determination with a wide linear range (0.1 to 70 μM) and a low detection line (0.028 μM). The recoveries for the determination of rutin in Coreopsis tinctoria and Buckwheat tea were 95.4%–106% and 95.3%–104%, respectively. This work successfully demonstrated the feasibility of Rh B as an internal reference probe.

A Paper-Based Electrochemical Sensor Based on Chain Structured ZIF-8@MWCNTs for Efficient Determination of Rutin

Rapid and accurate determination of rutin content in pharmaceuticals in an important issue. Here, a paper-based electrochemical sensor for in situ growth of chain ZIF-8 using multi-walled carbon nanotubes (MWCNTs) as a template was constructed by hydrothermal method. The results showed that the combination of ZIF-8 and MWCNTs not only promoted the dispersion of MWCNTs, but also made up for the conductivity of ZIF-8. Under optimum conditions, the Ipa was linearly related to the concentration of rutin in the range of 0.01–10 and 10–190 μM with a limit of detection (LOD) of 2.82 nM. Finally, the paper-based electrochemical sensor can be used for the electrochemical detection of rutin through the determination of rutin in pharmaceuticals.

Quantitative analysis of rutin in flavonoid-rich plant raw material by HPTLC

Introduction. Due to the increasing requirements for the quality of pharmaceutical products, the requirements for standardization of herbal medicines are also growing, which in turn necessitates the development of approaches to quality control of medicinal plant raw materials. The use of such an advanced method as high-performance thin-layer chromatography (HPTLC) will improve the quality of drug standardization, which is associated with the advantages of this method: minimized sample preparation, parallel determination of small volumes, elution time, high sensitivity, efficiency and speed. Objective. Quantitative determination of rutin by high-performance thin-layer chromatography, as well as comparison of the results of quantitative determination of the amount of flavonoids in terms of rutin by the spectrophotometric method. Material and methods. Medicinal plant raw materials – violet herb – were subjected to extraction using a developed method with 70 and 96% ethanol. Quantitative determination of rutin in extracts was carried out by HPTLC and spectrophotometry. Results. The developed method for the quantitative determination of rutin was validated in terms of linearity, specificity and accuracy. The difference in the extractive ability of the solvents used was shown. The percentage of rutin determined using the HPTLC method in an ethanol (96%) extract (3 µl) is 3.0826%, in an ethanol (70%) extract (3 µl) – 1.5911%. The percentage of rutin determined using the HPTLC method in an ethanol (96%) extract (5 µl) is 3.0894%, in an ethanol (70%) extract (5 µl) – 1.6064%. The percentage of total flavonoids in terms of rutin determined using the spectrophotometric method in the ethanol (96%) extract is 3.4289%, in the ethanol (70%) extract – 2.4176%. Conclusion. As a result of the study, a method for the quantitative determination of rutin was developed and validated, which can be used in the standardization of violet herb.

Wavelength-dependent photoelectrochemical response demonstrated by the determination of acetaminophen and rutin in differential molecularly imprinted polymers strategy

Herein, the excitation wavelength-dependent responses of the molecularly imprinted polymer (MIP) photoelectrochemical (PEC) sensors were investigated, using acetaminophen (AP), rutin (RT) and perfluorooctanoate (PFOA) as the model templates, pyrrole as functional monomer, CuInS2@ZnS/TiO2 NTs as the basic photoelectrode. With wavelength λ > 240 nm, the photocurrent of MIPPFOA enhanced at higher concentrations of PFOA. With increasing AP concentration, the photocurrents of MIPAP could decline with λ < 271 nm, not change at λ = 270 nm, or increase with λ > 270 nm. As RT concentration increased, the photocurrents of MIPRT could decrease (λ < 431 nm), not change (λ = 431 nm) or increase (λ > 431 nm). The PEC responses depend on the comprehensive interaction of two contrary mechanisms from the template molecules within the MIP membrane. The photocurrent is enhanced by the role of the electron donor for photo-generated holes but attenuated due to the steric hindrance effect and the excitation light intensity loss via absorption or scattering. The apparent molar absorption coefficient of AP and RT within MIP membranes are 9.1–19.4 folds of those measured from dilute solutions. By using a routine UV lamp as the light source, the photocurrents of MIPRT at 254 nm and MIPAP at 365 nm were used to determine RT and AP, with the detection limits of 5.3 and 16 nM, respectively. The interference from the non-specific adsorption of interferents on the surfaces of MIPAP and MIPRT was reduced by one order of magnitude via a differential strategy.

A novel electrochemical sensor based on a molecularly imprinted polymer for highly selective and sensitive determination of rutin from herbal supplements and plant extracts.

Rutin (RUT), a natural flavonoid with various beneficial pharmacological actions such as cardioprotective, antioxidant, anti-inflammatory, neuroprotective, etc., is found in the content of many plants that are consumed daily. Due to the healthful effects, RUT is also included in the composition of various herbal supplement samples. Therefore, it is highly important to develop a sensor with high selectivity and sensitivity to determine RUT in complex samples. In this study, it was aimed to take advantage of the cheap, easy, and sensitive nature of electrochemistry and, in addition, to improve the selectivity. For this purpose, the functional monomer selected in the fabricated molecularly imprinted polymer (MIP) was N-methacryloyl-L-aspartic acid (MA-Asp) while photopolymerization (PP) was applied as the polymerization route. After completing critical optimization steps, the developed sensor (MA-Asp@RUT/MIP-GCE) was characterized electrochemically and morphologically. As a result of analytical performance evaluation in standard solution, the linear response of the sensor was found in the concentration range between 1 and 10 pM with a detection limit of 0.269 pM. The recovery studies from plant extract and commercial herbal supplement samples emphasized accuracy and applicability. In imprinting factor studies figuring out quite good selectivity, molecules with a structure similar to RUT were selected as competitors to prove the affinity of the sensor against RUT. Consequently, the MA-Asp@RUT/MIP-GCE sensor offers a more sensitive and selective method thanks to its indirect analysis approach and also stands out with the diversity of its real sample application compared to other available studies.

Platforms of graphene/MXene heterostructure for electrochemical monitoring of rutin in drug and Tartary buckwheat tea

The use of two-dimensional heterostructure composite as electrode modification material has become a new strategy to improve the electrocatalytic activity and electroactive sites of electrochemical sensor. Herein, a soluble heterostructure, namely rGO-PSS@MXene, was designed and synthesized by integrating poly (sodium p-styrenesulfonate)-functionalized reduced graphene oxide into MXene nanosheets via ultrasonic method. The interactive heterostructure can effectively alleviate the self-stacking of MXene and rGO, endowing them with superior electron transfer capacity and large specific surface area, thereby producing prominent synergistic electrocatalytic effect towards rutin. In addition, the excellent enrichment effect of rGO-PSS@MXene for rutin also plays an important role through the electrostatic and π-π stacking interactions. The electrochemical characteristics of rutin on the sensor were examined in detail and a sensitive sensing method was proposed. Under optimized conditions, the method showed satisfactory linear relationship for rutin in the concentration range of 0.005–10.0 μM, with limit of detection of 1.8 nM (S/N = 3). The quantitative validation results in herbal medicine and commercial Tartary buckwheat tea were highly consistent with the labeled quantity and the results of HPLC determination, respectively, suggesting the sensor possessed excellent selectivity and accuracy. This proposed strategy for rutin determination is expected to expand the application of MXene heterostructure in electrochemical sensors, and is envisioned as a promising candidate for quality monitoring of drugs and foods.

Quantitative Determination and Source Variation of Rutin in Fagopyrum Tataricum

Buckwheat (Fagopyrum sp.) is a traditional crop of Uttarakhand (India) known for its high Rutin content, a bioflavonoid with significant medicinal properties. This study explores the quantitative measurement of Rutin in various buckwheat varieties grown in different regions of Uttarakhand using Thin Layer Chromatography (TLC) and High-Performance Thin Layer Chromatography (HPTLC). Twelve seed sources of Fagopyrum tataricum from the Garhwal and Kumaon regions, along with Fagopyrum esculentum seeds for comparison, were collected. Preliminary phytochemical screening of the extracts revealed the presence of flavonoids, alkaloids, tannins, and carbohydrates, with methanolic extracts showing higher concentrations. TLC analysis was employed to determine the most effective solvent system for Rutin separation, leading to the identification of three samples with substantial Rutin content. HPTLC was then used for quantitative Rutin assessment, with a calibration curve established using standard Rutin solutions. The three selected samples, along with the standard, were applied to HPTLC plates, and the Rutin content was measured based on peak areas and peak heights. The sample collected from Joshimath, Uttarakhand, known as "JOG," exhibited the highest Rutin content. This study provides valuable insights into the phytochemical composition of buckwheat varieties in Uttarakhand, which can inform the development of high-quality functional foods. Rutin, a potent antioxidant with various health benefits, underscores the significance of buckwheat as a naturally occurring functional food source. The results contribute to understanding the Rutin-rich potential of buckwheat grown in Uttarakhand and its applications in functional foods and nutraceuticals. Further research in this field can promote the cultivation and utilization of buckwheat as a source of this valuable bioactive compound with numerous health benefits.

Electrochemical Determination of Rutin on a Gold Microsphere—Taro Stalk Porous Carbon Modified Carbon Ionic Liquid Electrode (CILE)

As an abundant, inexpensive, and renewable biomass raw material, taro [Colocasia esculenta (L.) Schott] stalks were employed as a simple biochar template due to their porous structure. A composite composed of gold microsphere and taro stalk derived porous carbon (Au-MS@TSPC) was used to construct an electrochemical sensor for the sensitive determination of rutin. Under the optimized conditions, the sensor exhibited a wide linear range from 0.12 to 10.0 μmol·L−1 with a detection limit of 0.0265 μmol·L−1. The sensor demonstrated good selectivity and was applied to the determination of rutin in human serum with recoveries from 94.0% to 105.8%. The fabrication of Au-MS@TSPC in this study provides a promising approach for the subsequent utilization of taro stalks.

Synthesis of p-aminothiophenol functionalized graphene oxide/silver nanoparticles nanocomposite and its application for the determination of rutin in rose species flowers

This study used an electrochemical sensor based on a glassy carbon electrode that was designed with a nanocomposite of silver nanoparticles and p-aminothiophenol functionalized graphene oxide (Ag NPs/PATP/f-GO/GCE) to measure the level of rutin in actual samples taken from flowers of rose species. To create the Ag NPs/PATP/f-GO nanocomposite, the Ag NPs were synthesized using the traditional heating process and chemically combined with acid-treated GO and p-aminothiophenol. Ag NPs/PATP/f-GO nanocomposite's effective production was confirmed by X-ray diffraction (XRD), scanning electron microscope (SEM), and X-ray photoelectron spectroscopy (XPS) investigations. The construction of a sensitive, selective electrochemical system for the identification of rutin was made possible by the synergistic interaction of Ag NPs, PATP, and f-GO nanosheets, as demonstrated by electrochemical studies based on cyclic voltammetry (CV) and square wave voltammetry (SWV) measurements. The suggested sensor demonstrated a linear response of 5–1050 μM. It was determined that the sensitivity and limit of detection (LOD = 3σ/S, where S is the slope of the regression line and σ is the standard deviation of the intercept) were 0.02902 μA/μM and 17 nM, respectively. For the purpose of determining the level of rutin in a genuine sample made from flowers of a rose species (damascene rose) from Nanyang in Henan province, China, the suggested electrochemical rutin sensor's accuracy and capability were evaluated. Results showed that Ag NPs, PATP, f-GO, GCE may be used as dependable rutin sensors to monitor the rutin concentration in samples of rose species flowers with remarkable recovery values (higher than 98.32 %) and relative adequate accuracy (RSD less than 5.41 %).

Phytochemical profile analysis by LC-ESI-MSn and LC-HR-ESI-MS and validated HPLC method for quantification of rutin in herbal drug and products from leaves of Croton blanchetianus.

Phytochemical analysis of Croton blanchetianus leaves was performed by. After that, a high performance liquid chromatography method was developed and validated for the determination of rutin in herbal drug and products of C. blanchetianus. The separation was achieved on a C18 column, and the mobile phase was composed of ultrapure water and methanol (acidified with trifluoroacetic acid) with a gradient of 0.8ml/min. The method was validated following international guidelines. The chemical analysis revealed the presence of flavonoids. Among them rutin was used as the standard for validation. In the HPLC the presence of rutin was observed at 24.7min. The method was robust, with no significant variations, and linear in the range evaluated with R2 > 0.99. Regarding the matrix effect, it was possible to prove the absence of interference of the constituents in the herbal drug. The precision was determined with a relative standard deviation of <1.34%. The recovery results were achieved between 89.29 and 101.21%. Furthermore, with partial validation, the method was proved to be suitable for the liquid extract, dry extract and effervescent granules. Therefore, this study demonstrated that the method is effective for the quality control analysis of C. blanchetianus leaves and products.

An electrochemical sensor based on Ce-MOF-derived Ce-doped poly(3,4-ethylenedioxythiophene) composite for efficient determination of rutin in food

As a common antioxidant and nutritional fortifier in food chemistry, rutin has positive therapeutic effects against novel coronaviruses. Here, Ce-doped poly(3,4-ethylenedioxythiophene) (Ce-PEDOT) nanocomposites derived through cerium-based metal-organic framework (Ce-MOF) as a sacrificial template have been synthesized and successfully applied to electrochemical sensors. Due to the outstanding electrical conductivity of PEDOT and the high catalytic activity of Ce, the nanocomposites were used for the detection of rutin. The Ce-PEDOT/GCE sensor detects rutin over a linear range of 0.02–9 μM with the limit of detection of 14.7 nM (S/N = 3). Satisfactory results were obtained in the determination of rutin in natural food samples (buckwheat tea and orange). Moreover, the redox mechanism and electrochemical reaction sites of rutin were investigated by the CV curves of scan rate and density functional theory. This work is the first to demonstrate the combined PEDOT and Ce-MOF-derived materials as an electrochemical sensor to detect rutin, thus opening a new window for the application of the material in detection.

Application of Response Surface Methodology for the Development of an Innovative Stability Indicating UHPLC Method for the Simultaneous Determination of Rutin and Catechin in Corn Silk Extract Tablets

Application of Response Surface Methodology for the Development of an Innovative Stability Indicating UHPLC Method for the Simultaneous Determination of Rutin and Catechin in Corn Silk Extract Tablets

Study of the influence of ingredients on biopharmaceutical factors and pharmacological activity of a medicinal product with carrot extract and rutin

Aim. The work aimed to study the influence of the components of a soft rectal medicine with carrot root extract and rutin on biopharmaceutical parameters and its pharmacological activity.&#x0D; Materials and methods. The objects of the study were samples of soft pharmaceutical forms made on different bases. Pharmacological, biopharmaceutical, physicochemical and pharmacotechnological research methods were used in the study.&#x0D; The results. According to the data of organoleptic studies, determination of colloidal stability, and determination of pH, it was established that the studied samples were stable during the entire observation period. According to the data of rheological studies, it was established that all systems are thixotropic. However, the recovery time of the system is different, which is related to the physicochemical properties of auxiliary substances included in the samples. The performed spectral analysis of dialysate solutions of experimental samples of soft medicine indicates the possibility of quantitative determination of the number of flavonoids in dialysates in terms of rutin. The components of the base of the samples and the thick extract of carrot roots do not interfere with the determination of rutin in dialysates with pH 6.8 by the absorption spectrophotometry method at a wavelength of 352 nm. The analysis of the obtained results of the study of the release of rutin from samples into a phosphate buffer solution by dialysis through a semipermeable membrane shows that the complete release is provided by auxiliary substances used in the preparation of sample No. 4, which is an emulsion of the first kind. The obtained data from pharmacological studies on the dynamics of planimetric indicators on the model of stencil wounds in rats demonstrated a wound-healing effect in all the studied samples and the reference agent - Hemorol suppositories. However, using sample No. 4 in the treatment of a stencil wound promotes faster healing, which in clinical use can contribute to reducing the risk of infection, the spread of infection, and reducing the area of ​​the wound defect.&#x0D; Conclusions. According to the results of the complex studies, moderate advantages of sample No. 4 over the comparison drug and other samples have been established, determining the perspective of further research

Validation of HPLC method for quantitative determination of active compounds in Thai traditional herbal medicine to treat gastrointestinal disease

Quality control is essential for determining the quality and consistency of medicinal plant products before their use. Thatbunjob is a Thai herbal formulation included in the National List of Essential Medicines for treating gastrointestinal diseases. This study aimed to develop and validate a high-performance liquid chromatography method for determining active compounds in Thatbunjob. The main compounds, gallic acid, chebulagic acid, rutin, and eugenol, were determined following the International Conference on Harmonization (ICH) guidelines. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ), and found to be linear, with a correlation coefficient (R2) ranging from 0.9995 to 0.9998. The LOD and LOQ values calculated from the calibration curve ranged from 7.29 to 20.29 and 22.09 to 61.48 µg/mL, respectively. The method also exhibited acceptable precision, with relative standard deviation values lower than 2%. The accuracy of the method was evaluated based on the recovery percentages, and found to be within an acceptable range (90.12–105.39%). The validated protocol developed in this study was employed to analyze the concentration levels of gallic acid, chebulagic acid, rutin, and eugenol in eight different Thatbunjob products commercially available in Thailand. The performance parameters show that the developed and validated method is appropriate for the quantitative determination of gallic acid, chebulagic acid, rutin, and eugenol in Thatbunjob.

Determination of Rutin's antitumoral effect on EAC solid tumor by AgNOR count and PI3K/AKT/mTOR signaling pathway.

Rutin is one of the flavonoids found in fruits and vegetables. The PI3K/AKT/mTOR signaling pathway is critical for the life cycle at the cellular level. In current study, we purposed to demonstrate the antitumoral effect of rutin at different doses through the mTOR-signaling pathway and argyrophilic nucleolar regulatory region. EAC cells were injected subcutaneously into the experimental groups. 25 and 50 mg/kg Rutin were injected intraperitoneally to the animals with solid tumors for 14 days. Immunohistochemical, Real-time PCR and AgNOR analyzes were actualized on the taken tumors. When the rutin given groups and the tumor group were compared, the tumor size increase was detected to be statistically significant (p<0.05). In immunohistochemical analysis, a significant decrease was encountered in the AKT, mTOR, PI3K and F8 expressions especially in the groups administered 25 mg Rutin, in comparison with the control group (p<0.05). AgNOR area/nuclear area (TAA/NA) and average AgNOR number were determineted, and statistically important differences were detected between the groups in terms of TAA/NA ratio (p<0.05). There were significant statistical differences between the mRNA quantity of the PI3K, AKT1 and mTOR genes (p<0.05). In the in vitro study, cell apoptosis was evaluated with different doses of annexin V and it was determined that a dose of 10 µg/mL Rutin induced apoptosis (p <0.05). In our study, it was demonstrated in vivo and in vitro that Rutin has an anti-tumor effect on the development of solid tumors formed by both EAC cells.

Tailoring the electroactive area of carbon screen-printed electrodes by simple activation steps towards rutin determination

Tailoring the electroactive area of carbon screen-printed electrodes by simple activation steps towards rutin determination

Highly Selective and Sensitive Sensor Based IL and CMC-MWCNTs Nanocomposite for Rutin Determination

Rutin is a natural antioxidant flavonoid compound with anti-inflammatory, antioxidant, and antiviral effects that is used to prepare drugs with wide application in clinical treatment. Therefore, the quantitative detection of rutin has important practical significance. In this work, a novel electrochemical sensor based on glassy carbon electrodes (GCEs) modified with sodium carboxymethylcellulose (CMC), multi-walled carbon nanotubes (MWCNTs), and 1-butyl-3-methylimid (ionic liquid, IL) was developed for the super-sensitive detection of the flavonoid rutin. The properties of these modified materials were analyzed by transmission electron microscope (TEM), cyclic voltammograms (CVs), and electrochemical-impedance spectroscopy (EIS). CMC was used to disperse MWCNTs to further enhance their hydrophilicity and biocompatibility. The modified MWCNTs improved the sensitivity of rutin detection. The square-wave voltammetry (SWV) technique showed that the linear range of rutin concentration determination was 0.01 μM to 1 µM and 1 µM to 10 µM. The minimum concentration detection of rutin was 0.83 nM and 6.6 nM, respectively. The proposed sensor presented good selectivity for rutin and successfully analyzed rutin content in the pharmaceutical rutin tablets. These results are consistent with those measured by ultra-high-performance liquid chromatography (UHPLC). Therefore, this sensor has latent application value in the analysis of rutin in food and drug tablets and nutraceutical samples.

Self-assembly of gold nanoparticles on three-dimensional eggshell biological carbon fiber membranes: Non-enzymatic detection of rutin

A highly selective electrochemical sensing platform based on gold nanoparticles@three-dimensional carbonized eggshell fiber membrane (Au NPs@3D CEFM) was developed for non-enzymatic detection of rutin in the presence of ascorbic acid, dopamine and uric acid. The porous 3D connected-network CEFM with a pore size of ∼5 µm was prepared. Au NPs (∼10 nm) were uniformly anchored on the surface of the 3D CEFM by self-assembly. The porous 3D CEFM acts as a conductive platform and the regular small-sized Au NPs provide more active sites to significantly enhance the catalytic and selective properties of Au NPs@ 3D CEFM. Under the optimal conditions, the Au NPs@ 3D CEFM/ITO electrode exhibits an excellent sensitivity of 3.08 μA·μM−1·cm−2 and a low detection limit of 0.014 µM (S/N = 3) for the detection of rutin in 0–60 µM. Meanwhile, the electrode is also effectively used for the determination of rutin in actual biological samples and the recoveries for spiked samples ranged from 92.3% to 106.8%.