Changing the hydrological behavior of soils contaminated with oil has an essential effect on soil erosion and runoff generation processes. However, the applied and economically efficient and environmentally friendly approaches to tackle the contamination-related soil and water loss issues have yet to be found. Therefore, adopting appropriate conservation measures in petroleum-contaminated soils through the application of environmentally friendly is crucial. Soil microorganisms play a critical role in ecosystem function by improving soil's physical and chemical properties. Nonetheless, the effectiveness of such microorganisms in controlling soil loss on petroleum-contaminated soils has to be studied. Hence, the present study has elucidated the effects of surface inoculation of endemic soil microorganisms on exceeded soil loss from petroleum-contaminated soil. Towards that, soil samples were collected from Iran's Tehran Oil Refinery suburban area. The soil was further contaminated by 720 mL of gasoil, which was evenly sprayed superficially. The suitable bacteria, cyanobacteria, and fungi effective in soil and water conservation and available in the soil organism's micro-bank were selected, purified, propagated, and inoculated uniformly on the soil surface of small 0.25 m2-plots. The plots were then exposed to a simulated design rainfall with an intensity of 35 mm h−1 and 30 min duration placed on a slope of 25%. The performance comparison of solo bacteria, cyanobacteria, fungi, and consortium bacteria + fungi, bacteria + cyanobacteria, cyanobacteria + fungi, and bacteria + cyanobacteria + fungi inoculation on the soil contaminated with gasoil verified non-significant (p = 0.40) changes +0.44%, −81.26%, −87.56%, −39.25%, −92.98%, −96.09%, and −39.70% in soil loss They further caused non-significant (p = 0.47) increases of 26.65%, 56.32%, 74.53%, 27.33%, 78.94%, 87.66% and 62.01% in sediment concentration compared to those reported for the control treatment (i.e., uncontaminated soil and with initial level of contamination). The approach with further emphasis on the application of individual or combined use of cyanobacteria and fungi can be supposed to be a bio-strategy, sustainable, environmentally-friendly, and efficient technique for petroleum-contaminated soil conservation thanks to the ability of soil microorganisms in improving soil physical and chemical properties.
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