Running Wheel Activity In Rats Research Articles

Effects of voluntary exercise on the expression of browning markers in visceral and subcutaneous fat tissue of normotensive and spontaneously hypertensive rats

High physical activity is important to optimize the function of adipose tissue. Dysfunctional adipose tissue contributes to the development of metabolic stress, chronic inflammation, and hypertension. To improve our current understanding of the interaction between physical exercise and adipose tissue, we analyzed the effect of 10 months voluntary running wheel activity of rats on uncoupling protein (UCP) 1 negative white adipose tissue (visceral and subcutaneous adipose tissue, VWAT and SWAT). Analysis was performed via RT-PCR and immunoblot from adipose tissues depicted from adult normotensive and spontaneously hypertensive female rats. UCP1 negative VWAT differed from UCP1 positive WAT and brown adipose tissue (BAT) from interscapular fat depots, by lacking the expression of UCP1 and low expression of Cidea, a transcriptional co-activator of UCP1. High physical activity affected the expression of five genes in SWAT (Visfatin (up), RBP5, adiponectin, Cidea, and Nrg4 (all down)) but only one gene (Visfatin, up) in VWAT. Furthermore, the expression of these genes is differentially regulated in VWAT and SWAT of normotensive and spontaneously hypertensive rats (SHR) under sedentary conditions (UCP2) and exercise (Visfatin, Cidea, Nrg4). Keeping the animals after 6 months of voluntary exercise under observation for an additional period of 4 months without running wheels, Visfatin, Cidea, and Nrg4 were stronger expressed in VWAT of SHRs than in sedentary control rats. In summary, our study shows that SWAT is more responsible to exercise than VWAT.

Behavioural, histological and cytokine responses during hyperalgesia induced by carrageenan injection in the rat tail

We produced experimental inflammatory hyperalgesia by injecting carrageenan into the tail of Sprague–Dawley rats. We compared the rats' voluntary running wheel activity following carrageenan injection into the tail to that after carrageenan injection into the hind paw, the conventional site of inflammation, to identify whether the site of inflammatory-induced hyperalgesia altered voluntary activity. We also measured voluntary running before and after injection of carrageenan or saline into the tail or hind paw, and in separate groups of rats we measured the nociceptive response and the associated pro-inflammatory cytokine profiles following a carrageenan injection into the tail. Female rats were injected intradermally with either 2 mg carrageenan or saline into the dorsal surface of the tail. Withdrawal responses to noxious heat (49 °C water), and punctate mechanical (electronic anaesthesiometer) challenges were recorded in 12 rats for 3 days before and 1 h to 48 h after injection. In a separate group of rats, interleukin (IL)-1β, IL-6, tumour necrosis factor-alpha (TNF-α) and cytokine-induced neutrophil chemoattractant (CINC-1) concentrations were measured in plasma and tail tissue samples taken at the site of injection, 3 h, 6 h and 24 h after injections. Voluntary wheel running was reduced significantly following carrageenan injection into the hind paw compared to that after saline injection into the hind paw. Carrageenan injection into the tail did not result in significant reduction in wheel running compared to that after saline injection into the tail. Both thermal and mechanical hyperalgesia were present after carrageenan injection into the tail ( P < 0.01, ANOVA). The hyperalgesia at the site coincided with significant increases in TNF-α, IL-1β, IL-6 and CINC-1 tissue concentrations, peaking 6 h after carrageenan injection ( P < 0.01, ANOVA). We conclude that carrageenan injection into the tail produces inflammatory hyperalgesia with underlying pro-inflammatory cytokine release, but does not affect voluntary running wheel activity in rats.

Circadian rhythm of drinking and running-wheel activity in rats with 6-hydroxydopamine lesions of the ventral tegmental area

Circadian rhythms in drinking and running-wheel (locomotor) activity of rats with 6-hydroxydopamine (6-OHDA, 4 μg/2 μl per rat)-induced lesions in the ventral tegmental area (VTA) were examined under a light–dark (LD) cycle and constant dim light (5 lux). Under the LD cycle, the length of the locomotor activity period was decreased during the dark, and increased during the light period in the lesioned rats. Under the constant dim light conditions, the free-running circadian period (τ) of drinking and activity rhythm was longer in lesioned rats than in sham-operated controls. The elongation of the circadian period was accompanied by decrements in activity. These observations suggest that the mesolimbic dopaminergic system modulates rhythms in circadian drinking and locomotor activity.

Light-dark patterns in running-wheel activity in rats during chronic administration of theophylline

Running-wheel activity for 24 hr and activity patterns were studied during chronic theophylline administration. Theophylline altered the normal relations between activity level and illumination. Dark-time activity was decreased to approximately 50% and 24 hr activity was unaffected. These observations were consistent with previous results showing that theophylline suppressed dark-time feeding but had no effect on 24 hr food intake. A possible mechanism to account for these results may be dependent on levels and turnover of brain norepinephrine.

Effect of alcohol on running-wheel activity in rats

Groups of 10 rats were given either 15% w/v ethanol (ETOH) or water and ad lib food in activity wheels which were allowed to move freely or were fixed for a 21-day period. There was no overall difference in activity between the ETOH or water groups; however, a significant increase in activity was found in the ETOH group during the first 6 experimental days. This effect may have been due to a significant reduction in the amount of food and fluid consumed by the animals as well as to weight loss during the initial stage.