Ruminant Animal Species Research Articles

Effect of Feeding Solid Multi-Nutrient Blocks on Feed Intake, Nutrient Utilization and Haemato-Biochemical Profile of Crossbred Calves

Background: Supplementation of urea molasses mineral block (UMMB) showed promising results in improving the nutrient utilization and the productivity of animals. This offers several advantages such as easy transport, storage and handling and reduced risks of poisoning as compared with other approaches, such as giving a small amount of urea in drinking water, sprinkling of urea solution on fibrous feeds before feeding, or urea-ammonization of crop residues. These advantages of solid multi-nutrient blocks (SMB) opened up new vista to enhance productivity in terms of increased milk and meat production and higher reproductive efficiency in ruminant animal species. Keeping the above facts in view, this study was undertaken to determine the effect of feeding solid multinutrient blocks on feed intake, nutrient utilization and haemato-biochemical profile of crossbred calvesMethods: In this study twenty four crossbred male calves of about 14-17 months of age were divided into four groups of six animals in each group. All animals were supplied wheat straw ad lib. In addition, animals in all groups were fed a concentrate mixture to meet out their nutrient requirement. Additionally, 30 % of concentrate mixture (on CP basis) was replaced by solid multi-nutrient blocks (SMB-I), SMB-II and SMB-III in groups T1, T2 and T3, respectively. After 60 days of feeding a metabolism trial of 6 days was conducted to assess the nutrient utilization of animals. Blood samples were collected at 0, 75, 150 day of experimental feeding to determine hematological parameters, enzymes, biochemical parameters and serum mineral concentration.Result: The intake and digestibility of DM, OM, EE, NDF and ADF were comparable among 4 groups. The CP intake was significantly higher in SMB supplemented groups as compared to control but there was no significant difference in CP digestibility among 4 groups. All the animals in four groups were on positive nitrogen, calcium and phosphorus balance. Haematobiochemical parameters like Hb, PCV, glucose, total protein, globulin, A:G, SGOT and SGPT showed non-significant differences (p greater than 0.05) among the groups, however, periodical serum concentrations of albumin, urea and creatinine was higher in crossbred calves at 75 and 150 days of experiment in comparison to 0 day. On basis of present results, it may be concluded that supplementation of SMB can replace 30% crude protein of concentrate mixture without any adverse effect on feed intake, digestibility, nutrient utilization and blood biochemical profile of crossbred calves.

Meta-analysis of global livestock urine-derived nitrous oxide emissions from agricultural soils.

Nitrous oxide (N2O) is an air pollutant of major environmental concern, with agriculture representing 60% of anthropogenic global N2O emissions. Much of the N2O emissions from livestock production systems result from transformation of N deposited to soil within animal excreta. There exists a substantial body of literature on urine patch N2O dynamics, we aimed to identify key controlling factors influencing N2O emissions and to aid understanding of knowledge gaps to improve GHG reporting and prioritize future research. We conducted an extensive literature review and random effect meta‐analysis (using REML) of results to identify key relationships between multiple potential independent factors and global N2O emissions factors (EFs) from urine patches. Mean air temperature, soil pH and ruminant animal species (sheep or cow) were significant factors influencing the EFs reviewed. However, several factors that are known to influence N2O emissions, such as animal diet and urine composition, could not be considered due to the lack of reported data. The review highlighted a widespread tendency for inadequate metadata and uncertainty reporting in the published studies, as well as the limited geographical extent of investigations, which are more often conducted in temperate regions thus far. Therefore, here we give recommendations for factors that are likely to affect the EFs and should be included in all future studies, these include the following: soil pH and texture; experimental set‐up; direct measurement of soil moisture and temperature during the study period; amount and composition of urine applied; animal type and diet; N2O emissions with a measure of uncertainty; data from a control with zero‐N application and meteorological data.

Discovery of Enteric Pathogens in the Alaskan Subsistence Diet

Abstract Objectives Transmission of enteric pathogens from food ingestion is an ongoing public health concern, with commensal bacteria in ruminant animal species causing human disease. Enteric pathogens Salmonella, Shigella, and Shiga toxin producing Escherichia coli (STEC) have been isolated from domesticated animals. However, the Alaskan subsistence diet relies on wild game, such as reindeer, caribou, and moose for their food supply. Research concerning enteric pathogens in wildlife has not established. Therefore, we conducted a pilot survey on moose and reindeer to determine potential enteric pathogen transfer risk. Methods Between July 2018 and January 2019, we collected 72 fecal samples from reindeer and moose migrating in Fairbanks, Anchorage, and the Matanuska Valley. Samples were cultured for enteric pathogens, including E coli 0157, using standard clinical microbial process. Phenotypic Shiga toxin production was verified by enzyme immunoassay. Results Reindeer were statistically significant for enteric pathogens when compared to moose (P < .05) Eighty percent of the reindeer population were colonized for either Shigella, Yersinia, or Shiga toxin-producing E coli, with 20% positive for multiple pathogens. Non-0157 Shiga toxin production was observed in 30% of reindeer samples, generated by a sorbitol fermenting E coli. In contrast, moose population showed a near absence of enteric pathogens with only 5% positive for Shigella. Salmonella was not identified in either animal. Conclusion Reindeer, moose, and caribou meat are prominent in the Alaskan subsistence diet. Although moose had limited enteric pathogen colonization, reindeer were significant for transmission risk, including non-0157 Shiga toxin producing E coli, which has been linked to hemolytic uremic syndrome. Isolation of a non-0157 STEC in wildlife indicates environmental colonization. Because reindeer and caribou are closely linked in diet and migration, Alaska clinical laboratories should screen for enteric pathogens, including non-0157 Shiga toxins.

Characterisation of the bacterial community in the gastrointestinal tracts of elk (Cervus canadensis)

The resident bacteria of the gastrointestinal tract (GIT) and the behaviour of these microbes have been poorly characterised in elk as compared to other ruminant animal species such as sheep and cattle. In addition, most microbial community studies of deer gut have focused on rumen or faeces, while other parts of the GIT such as the small and large intestine have received little attention. To address this issue, the present study investigated the diversity of the GIT bacterial community in elk (Cervus canadensis) by 16S rRNA pyrosequencing analysis. Eight distinct GIT segments including the stomach (rumen, omasum, and abomasum), small intestine (duodenum and jejunum), and large intestine (cecum, colon, and rectum) obtained from four elks were examined. We found that bacterial richness and diversity were higher in the stomach and large intestine than in the small intestine (P < 0.05). A total of 733 genera belonging to 26 phyla were distributed throughout elk GITs, with Firmicutes, Bacteroidetes, and Proteobacteria identified as the predominant phyla. In addition, there was spatial heterogeneity in the composition, diversity, and species abundance of microbiota in the GIT (P < 0.0001). To the best of our knowledge, this is the first study to characterise bacterial communities from eight GIT regions of elk by 16S rRNA pyrosequencing.

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis.

The Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of antimicrobial susceptibility, the presence of femA gene, the genetic relationships among isolates of S. aureus obtained from milk originating from flocks diagnosed with subclinical mastitis in nine rural properties in the northern of Minas Gerais State. To this end, 498 samples of bovine milk tested positive for the California mastitis test (CMT) were subjected to morphological methods and biochemical patterns for microbiological presumptive identification of S. aureus. The PCR test with the genetic marker femA was used to confirm the species S. aureus. All the 26 isolates presumptively identified as S. aureus amplified a fragment of 132 bp corresponding to the femA gene. The profile of antimicrobial susceptibility was performed according to the disk-diffusion methodology and two isolates were susceptible to all the antibiotics tested. The drug multiresistence was found in 80.76% of the isolates. The determination of the genetic profile and the clonal relationship among the isolates was performed by the method of DNA RAPD-PCR polymorphism. The S. aureus isolates were divided into two groups with 26 distinct subgroups. The analysis of RAPD-PCR showed no genetic diversity among them, heterogeneous profile and absence of clonality.

Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) in the plasma and milk of ruminant animals

Milk contains a variety of proteins and peptides that possess biological activity. Growth factors, such as growth hormone, insulin-like, epidermal and nerve growth factors are important milk components which may regulate growth and differentiation in various neonatal tissues and also those of the mammary gland itself. We have recently shown that pituitary adenylate cyclase-activating polypeptide (PACAP), an important neuropeptide with neurotrophic actions, is present in the human milk in much higher concentration than in the plasma of lactating women. Investigation of growth factors in the milk of domestic animals is of utmost importance for their nutritional values and agricultural significance. Therefore, the aim of the present study was to determine the presence and concentration of PACAP in the plasma and milk of three ruminant animal species. Furthermore, the presence of PACAP and its specific PAC1 receptor were investigated in the mammary glands. Radioimmunoassay measurements revealed that PACAP was present in the plasma and the milk of the sheep, goat and the cow in a similar concentration to that measured previously in humans. PACAP38-like immunoreactivity (PACAP38-LI) was 5–20-fold higher in the milk than in the plasma samples of the respective animals, a similar serum/milk ratio was found in all the three species. The levels did not show significant changes within the examined 3-month-period of lactation after delivery. Similar PACAP38-LI was measured in the homogenates of the sheep mammary gland samples taken 7 and 30 days after delivery. PAC1 receptor expression was detected in these udder biopsies by fluorescent immunohistochemistry suggesting that this peptide might have an effect on the mammary glands themselves. These data show that PACAP is present in the milk of various ruminant domestic animal species at high concentrations, the physiological implications of which awaits further investigation.

Tolerance of ruminant animals to high dose in-feed administration of a selenium-enriched yeast1

The objective of the study was to determine if there were adverse effects on animal health and performance when a range of ruminant animal species were fed at least 10 times the maximum permitted European Union (EU) Se dietary inclusion rate (0.568 mg of Se/kg of DM) in the form of Se-enriched yeast (SY) derived from a specific strain of Saccharomyces cerevisiae, CNCM I-3060. In a series of studies, dairy cows, beef cattle, calves, and lambs were offered a control diet that contained no Se supplement or a treatment diet that contained the same basal feed ingredients plus a SY supplement that increased total dietary Se from 0.15 to 6.25, 0.20 to 6.74, 0.15 to 5.86, and 0.14 to 6.63 mg of Se/kg of DM, respectively. The inclusion of the SY supplement increased (P < 0.001) whole-blood Se concentrations, reaching maximum mean values of 716, 1,505, 1,377, and 724 ng of Se/mL for dairy cattle, beef cattle, calves, and lambs, respectively. Seleno-methionine accounted for 10% of total whole-blood Se in control animals, whereas the proportion in SY animals ranged between 40 and 75%. Glutathione peroxidase (EC 1.11.1.9) activity was greater (P < 0.05) in SY animals compared with controls. A range of other biochemical and hematological parameters were assessed, but few differences of biological significance were established between treatment groups. There were no differences between treatment groups within each species with regard to animal physical performance or overall animal health. It was concluded that there were no adverse effects on animal health, performance, and voluntary feed intake with the administration of at least 10 times the EU maximum, or approximately 20 times the US Food and Drug Administration permitted concentration of dietary Se in the form of SY derived from a specific strain of Saccharomyces cerevisiae CNCM I-3060.

VerotoxigenicEscherichia colifrom animals, humans and foods: who's who?

Verocytotoxigenic (shigatoxigenic) and enterohaemorrhagic Escherichia co/i, VTEC (STEC) and EHEC, produce a toxin active on Vero cells in vitro. VTEC and EHEC have been isolated from humans and different animal species, mainly ruminants and pigs. The verocytotoxins, also named shiga toxins (Stx), are active in vivo on the endothelial cells of the blood vessels of the gastro-intestinal mucosa, the kidneys, the brain, and other tissues of humans and piglets, leading to fluid leakage or haemorrhages. Conversely, their role in diseases of young ruminants remains unclear. Adult ruminants can also act as asymptomatic carriers of VTEC and EHEC strains similar to those causing diseases in humans. And they are incriminated as an important source of direct or indirect contamination of humans by the most famous EHEC strain belonging to the O157:H7 serotype, through faecal contamination of either foods of animal origin, or other foodstuffs (fruit, vegetables, etc.), or the environment. But dozens of non-O157 human and ruminant VTEC and EHEC strains with similar general and virulence-associated properties, have been described, whose epidemiology is much less well understood. The purpose of this review manuscript is to describe and compare the properties of human, ruminant and food VTEC and EHEC strains.

Identification of Interferon-Tau at the Maternal-Fetal Interface in Shiba Goats.

Interferon-tau (IFNτ) is a primary factor secreted by the conceptus responsible for maternal recognition of pregnancy in ruminant animal species. Although IFNτ is well characterized in ovine and bovine species, only minute data are available for caprine IFNτ species (cIFNτ) and there is not much information available on Shiba goat IFNτ. The aim of the present investigation was to establish a new animal model system for the study of maternal-fetal communication in Shiba goats. In order to detect Shiba goat IFNτ expression, recombinant ovine IFNτ (oIFNτ) proteins were initially prepared and were used to prepare an antibody against oIFNτ in rabbits. This antiserum was used to execute immunohistochemical analysis with tissue sections prepared from Shiba goat whole uteri fixed and embedded in paraffin. Conceptus tissues exhibited distinctive staining for the presence of a protein(s) related to oIFNτ. Using the primers designed from oIFNτ cDNA, DNA sequences for potential Shiba goat IFNτ were then amplified. Nucleotide and deduced amino acid sequences of the Shiba goat equivalent were analyzed and compared to IFNτ of other species. Three kinds of nucleotide sequences close to oIFNτ and cIFNτ were identified: Two of which were almost equivalent to oIFNτ and cIFNτ, and one of which had lower homology in nucleotide and amino acid sequences. Deduced amino acid sequences of three types of clones were conserved in each position distinctive for IFNτ family. These data indicate that three types of genes identified are IFNτ homologues of Shiba goats. These data suggest that nucleotide probes derived from these genes could be used to further study biological significance and transcriptional regulation of IFNτ during periods of peri-implantation.

Isolation of satellite cells from ovine skeletal muscles

We describe our protocol for isolating myogenic cells from the semimembranous muscles of lamb. Cultured cells display properties similar to myogenic satellite cells isolated from other species; similar characteristics include the ability to proliferate, differentiate, and fuse to form myotubes in primary cell culture. Using the described procedures, we provide the first documentation of satellite like cells existing in postnatal skeletal muscle of a ruminant animal species.