Rugosa Thunb Research Articles

Rosehip (Rosa rugosa Thunb.) ethanol extract ameliorates liver fibrosis through upregulation of the PPAR signaling pathway

The objective of this study was to examine the effect of rosehip (Rosa rugosa Thunb.) ethanol extract (RRE) on liver fibrosis in mice and the underlying molecular mechanisms. RRE has no acute oral toxicity, and the MTD in mice is 46.14 g/kg. Histopathological analysis showed that RRE significantly ameliorated inflammation, necrosis, and apoptosis of hepatocytes and collagen deposition caused by CCl4. Further assays indicated that RRE significantly suppressed the production of MDA while markedly increasing the levels of SOD and GSH in the liver. The AST, ALT, IL-6, IL-1β, TNF-α, HA, LN, PCIII, and IVC levels in serum were decreased by RRE. Transcriptome analysis shows that RRE significantly upregulates the PPAR signaling pathway. The RT-q PCR results further confirmed the RRE up-regulating Pparγ, Rxrα, and Plin5 in the PPAR signaling pathway. The Western blot and immunohistochemical results indicate that RRE upregulated PPARγ, RXRα, and Perilipin 5 while downregulating α-SMA and COL1A1 expression. The results indicate that RRE can ameliorate liver fibrosis in mice by upregulating the PPAR signaling pathway.

Total flavonoids from Rosa rugosa Thunb.: A comprehensive review of its extraction and purification process, chemical composition, biological effect and applications.

Rosa rugosa Thunb. is a kind of traditional Chinese medicine with homology of medicine and food in China for a long history. The total flavonoids from R. rugosa Thunb. (TFR), possessing antioxidant, anti-tumor, antidepressant, and antibacterial activities, can effectively prevent and treat cardiovascular, hyperglycemia, hyperlipidemia, and other diseases. However, few studies have focused on TFR analyses and no comprehensive review has yet been published. In order to provide a theoretical foundation and research direction for further study and development, this paper methodically reviewed the extraction and purification procedure, chemical composition, biological activity, toxicology, and application in food of TFR on the basis of previous research. A total of 102 distinct flavonoids have been isolated from R. rugosa Thunb. Further pharmacological activities and chemical analyses research will contribute to the development of TFR products in R. rugosa Thunb.

Micronized Rose Petal Powder: A Valuable Edible Floral Food Ingredient Containing Bioactive Compounds

Flower petals, as byproducts, provide significant health benefits and can be used in food production. In this study, the impact of the micronization process using a ball mill on the properties of micronized powders derived from wild rose petals of the rugosa variety (Rosa rugosa Thunb.) was examined. The micronized rose powders were subjected to an investigation regarding their particle size, color, molecular characterization (FTIR), electronic nose procedure and antioxidant potential. The study found that micronization considerably reduced d50 particle dimensions from 98.6 µm to 39.9 µm. An FTIR analysis revealed the presence of characteristic (2980, 1340, and 1225 cm−1) bands. The hydrolysable tannins are the most abundant polyphenolic chemicals in rose powders, followed by anthocyanins. Rose powders are an extremely valuable antioxidant raw material due to their high total phenol content (71.8 mg GAE/g), which increased by approximately 26% after micronization. The antioxidant activity, as determined by ABTS•+, DPPH• and FRAP, is likewise very high. The intensity of volatile chemicals decreased in powders after micronization.

Comparative analysis of antidepressant-like effects of five herbal essential oils on mice with reserpine-induced depression

The antidepressant-like effects of five herbal essential oils (EOs), Jasminum sambac (Linn.) Aiton essential oil (JSEOs), Magnolia denudata Desr. essential oil (MDEOs), Rosa rugosa Thunb. essential oil (RREOs), Aloysia citriodora Ortega ex Pers. essential oil (ACEOs) and Abies balsamea (L.) Mill. essential oil (ABEOs) were evaluated. By virtue of a reserpine-induced depression mouse model, the antidepression activities and mechanisms of these EOs were explored. The results indicated that the inhalation of EOs significantly ameliorated depression-like behaviors in reserpine-induced depression mice, including a decrease in anxiety and immobility time. JSEOs and ABEOs significantly attenuated reserpine-induced depression-like behaviors, particularly the decreased body weight and immobility time, as determined by tail suspension and forced swimming tests. Furthermore, RT-qPCR and western blotting analyses indicated that the EOs influenced brain-derived neurotrophic factor (BDNF), serotonin1A (5-HT1A), and inflammatory cytokine levels. ACEOs attenuated reserpine-induced hippocampal and neuronal damage, significantly increased glucocorticoid receptor (GR) and 5-HT1A levels, and suppressed the inflammatory response. These results provide new insights into the antidepressant effects of herbal EOs and suggest that the five EOs evaluated in this study are potential nutraceuticals for the treatment of depression.

Molecular cloning and characterization of farnesyl diphosphate synthase from Rosa rugosa Thunb associated with salinity stress.

Rosa rugosa, a renowned ornamental plant, is cultivated for its essential oil containing valuable monoterpenes, sesquiterpenes, and other compounds widely used in the floriculture industry. Farnesyl diphosphate synthase (FPPS) is a key enzyme involved in the biosynthesis of sesquiterpenes and triterpenes for abiotic or biotic stress. In this study, we successfully cloned and characterized a full-length FPPS- encoding cDNA identified as RrFPPS1 using RT-PCR from R. rugosa. Phylogenetic analysis showed that RrFPPS1 belonged to the angiosperm-FPPS clade. Transcriptomic and RT-qPCR analyses revealed that the RrFPPS1 gene had tissue-specific expression patterns. Subcellular localization analysis using Nicotiana benthamiana leaves showed that RrFPPS1 was a cytoplasmic protein. In vitro enzymatic assays combined with GC-MS analysis showed that RrFPPS1 produced farnesyl diphosphate (FPP) using isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to provide a precursor for sesquiterpene and triterpene biosynthesis in the plant. Additionally, our research found that RrFPPS1 was upregulated under salt treatment. These substantial findings contribute to an improved understanding of terpene biosynthesis in R. rugosa and open new opportunities for advancements in horticultural practices and fragrance industries by overexpression of the RrFPPS1 gene in vivo increased FPP production and subsequently led to elevated sesquiterpene yields in the future. The knowledge gained from this study can potentially lead to the development of enhanced varieties of R. rugosa with improved aroma, medicinal properties, and resilience to environmental stressors.

In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts.

Malassezia furfur (M. furfur) is a prevalent dermatophyte that significantly impairs patients' quality of life. This study aimed to evaluate the synergistic antifungal effects of combined extracts from Rosa rugosa Thunb. (MG) and Coptidis Rhizoma (HL) against M. furfur, both in vitro and in vivo. High-performance liquid chromatography (HPLC) was used to identify the major active compounds present in MG and HL. The antifungal activity of the combined Meilian extract (ML) was assessed using the checkerboard method and time-kill curves. Microstructural alterations in the fungi were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The impact of the extracts on the fungal cell membrane was investigated through propidium iodide staining, protein concentration assays, and ergosterol quantification. Transcriptomic analysis was conducted to elucidate the molecular mechanisms underlying the effects of the extracts. Furthermore, the synergistic antifungal effects of ML were evaluated in a mouse model of seborrheic dermatitis induced by M. furfur. The study demonstrated that the combined application of MG and HL significantly affected the integrity of the M. furfur cell membrane and potentially modulated its formation processes. In the M. furfur-induced seborrheic dermatitis model, ML exhibited synergistic antifungal effects and effectively alleviated skin inflammation. These findings provide an important theoretical basis for understanding the antifungal mechanisms of ML and its potential application in dermatological therapy.

The Herbal Combination Shu Gan Jie Yu Regulates the SNCG/ER-a/AKT-ERK Pathway in DMBA-Induced Breast Cancer and Breast Cancer Cell Lines Based on RNA-Seq and IPA Analysis.

Soothing the liver (called Shu Gan Jie Yu in Chinese, SGJY) is a significant therapeutic method for breast cancer in TCM. In this study, 3 liver-soothing herbs, including Cyperus rotundus L., Citrus medica L. var. sarcodactylis Swingle and Rosa rugosa Thunb. were selected and combined to form a SGJY herbal combinatory. To investigate the inhibiting effect of SGJY on breast cancer in vivo and vitro, and to explore the potential mechanisms. SGJY herbal combination was extracted using water. A breast cancer rat model was developed by chemical DMBA by gavage, then treated with SGJY for 11 weeks. The tumor tissue was preserved for RNA sequencing and analyzed by IPA software. The inhibition effects of SGJY on MCF-7 and T47D breast cancer cells were investigated by SRB assay and cell apoptosis analysis, and the protein expression levels of SNCG, ER-α, p-AKT and p-ERK were measured by western blotting. SGJY significantly reduced the tumor weight and volume, and the level of estradiol in serum. The results of IPA analysis reveal SGJY upregulated 7 canonical pathways and downregulated 16 canonical pathways. Estrogen receptor signaling was the key canonical pathway with 9 genes downregulated. The results of upstream regulator analysis reveal beta-estradiol was the central target; the upstream regulator network scheme showed that 86 genes could affect the expression of the beta-estradiol, including SNCG, CCL21 and MB. Additionally, SGJY was verified to significantly alter the expression of SNCG mRNA, CCL21 mRNA and MB mRNA which was consistent with the data of RNA-Seq. The inhibition effects of SGJY exhibited a dose-dependent response. The apoptosis rates of MCF7 and T47D cells were upregulated. The protein expression of SNCG, ER-α, p-AKT and p-ERK were all significantly decreased by SGJY on MCF-7 and T47D cells. The results demonstrate that SGJY may inhibit the growth of breast cancer. The mechanism might involve downregulating the level of serum estradiol, and suppressing the protein expression in the SNCG/ER-α/AKT-ERK pathway.

Search for Toxic Trace Elements in Rosa rugosa Thunb. By Instrumental Neutron Activation Analysis: Accumulation and Responses to Exposure

Species of the genus Rosa L. are widely used in urban gardening, and they are of considerable interest as plants accumulating heavy metals in an urban environment. Literature data are ambiguous about whether these species accumulate trace and toxic elements. Its fruits are used as medicinal raw materials in folk and official medicine. The purpose of the study is to determine whether this type of wild rose accumulates toxic metals (as well as various macro- and microelements), if so, which ones and in which organs, and to what extent raw materials of the plant can be used. For the first time, multi-element instrumental neutron activation analysis at the reactor IBR-2 of FLNP JINR in Dubna, Russia, was applied to examine accumulation of major and trace elements in various organs of Rosa rugosa Thunb. (Rosaceae family), namely, roots, leaves, fruits and seeds. A total of 33 elements (Al, Ca, Cl, I, Mg, Mn, V, As, Br, K, La, Na, Mo, Sm, U, W, Ba, Ce, Co, Cr, Cs, Fe, Hf, Ni, Rb, Sb, Sc, Sr, Ta, Tb, Th, Yb, and Zn) were determined. Samples were taken in the summer-autumn period of 2020, both in the city of St. Petersburg and on the coast of the Baltic Sea, Russia. This research found no critical accumulation of trace and toxic elements in different organs of R. rugose, but revealed quite high accumulation of Fe and Co in urban transport highway area.

Essential oils for treating anxiety: a systematic review of randomized controlled trials and network meta-analysis.

The findings of clinical studies exploring essential oils (EOs) for anxiety remain disputed, and no studies have yet clarified the differences in the efficacy of EOs. The purpose of the study was to directly or indirectly compare the efficacy of different types of EOs on anxiety by pooling the results of randomized controlled trials (RCTs). PubMed, Cochrane Library, Embase, Scopus, Web of Science and the Cochrane Central Register of Controlled Trials (CENTRAL) databases were searched from inception to November 2022. Only full texts of RCTs that investigated the effects of EOs on anxiety were included. The trial data were extracted and the risk of bias was assessed by two reviewers independently. Pairwise meta-analysis and network meta-analysis were performed by Stata 15.1 or R 4.1.2 software. Forty-four RCTs (fifty study arms) involving 10 kinds of EOs and 3419 anxiety patients (1815 patients in EOs group and 1604 patients in control group) were included. Pairwise meta-analyses showed that EOs were effective in reducing State Anxiety Inventory scores (SAIS) [WMD = -6.63, 95% CI-8.17, -5.08] and Trait Anxiety Inventory scores (TAIS) [WMD = -4.97, 95% CI-6.73, -3.20]. Additionally, EOs could decrease systolic blood pressure (SBP) [WMD = -6.83, (95% CI -10.53, -3.12), P < 0.001] and heart rate (HR) [WMD = -3.43, (95% CI -5.51, -1.36), P < 0.001]. Network meta-analyses demonstrated that regarding the outcome of SAIS, Jasminum sambac (L.)Ait. (jasmine) was the most effective with a weighted mean difference (WMD) of-13.61 (95% CrI-24.79, -2.48). Followed by Citrus (citrus aurantium L.), which had a WMD of-9.62 (95% CrI-13.32, -5.93). Moderate effect sizes were observed for Rosa rugosa Thunb. (damask rose) (WMD = -6.78, 95% CrI-10.14, -3.49) and Lavandula angustifolia Mill. (lavender) (WMD = -5.41, 95% CrI-7.86, -2.98). Regarding the results of TAIS, citrus aurantium L. was the best ranked intervention with a WMD of-9.62 (95% CrI-15.62, -3.7). Moderate-to-large effect sizes were observed for Citrus limon (L.) Burm. F. (lemon) (WMD:-8.48; 95% CrI-16.67, -0.33) and lavender (WMD:-5.5; 95% CrI-8.7, -2.46). According to the comprehensive analysis, EOs are effective in reducing both state anxiety and trait anxiety, and citrus aurantium L. essential oil seems to be the most recommended type of EO for treating anxiety because of its significant effects in reducing SAIS and TAIS. https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42022331319.

Effects of invasive Rosa rugosa on Baltic coastal dune communities depend on dune age

Rosa rugosa Thunb. (Japanese Rose) is one of the most invasive species in Europe. It spreads spontaneously in coastal areas of western, central and northern Europe, posing a threat to dune habitats, including those indicated in the EU Habitats Directive as particularly valuable. R. rugosa has already been reported to displace native plants and alter soil properties. However, little is known about how these effects are mediated by the habitat context or the invader condition (health, ontogenetic stage). This study addressed that gap by examining vegetation and soil in 22 R. rugosa-invaded sites, half of which were in yellow dunes and the other half in grey dunes, i.e. two habitats representing the earlier and later stages of dune succession. The study was conducted on the Hel Peninsula (Poland’s Baltic coast). R. rugosa had a significant impact on dune vegetation, but the impact was strongly dependent on the habitat type. In the yellow dune sites, R. rugosa outcompeted most resident plant species, which translated into a strong decline in their total cover and richness. The invasion was almost not accompanied by changes in soil properties, suggesting that it affected the resident vegetation directly (through space takeover and shading). In the grey dunes, R. rugosa caused a shift in species composition, from that characteristic of open communities to that typical of forests. In this habitat, a significant increase in the soil organic layer thickness under R. rugosa was also observed, which means that both direct and indirect effects of the invasion on the vegetation should be assumed. Finally, a negative relationship was found between the total chlorophyll content in R. rugosa leaves and the parameters of resident plant communities, showing that the invasion effects can vary not only across habitats, but also with the condition of the invader. The results may have practical implications for managing R. rugosa invasions in coastal sand dune systems. Since R. rugosa accelerates grey dune succession, protecting this habitat may be more urgent and, at the same time, more complicated than protecting dunes at the earlier stages of development.

Comparative Chemical Profiling and Citronellol Enantiomers Distribution of Industrial-Type Rose Oils Produced in China.

The chemical composition and aroma profile of industrial essential oils (EOs) from species of rose grown in China, including the native Kushui rose (R. sertata × R. rugosa) and R. rugosa Thunb. cv. Plena, and the recently introduced Damask rose (R. damascena Mill.), were studied in comparison by means of GC/MS and GC-FID. More than 150 individual compounds were detected in Chinese rose samples, of which 112 were identified and their quantitative content determined, representing 88.7%, 96.7% and 97.9% of the total EO content, respectively. It was found that the main constituents of the Chinese rose EOs were representatives of terpenoid compounds (mono- and sesquiterpenoids, predominantly) and aliphatic hydrocarbons. Comparative chemical profiling revealed different chemical composition and aroma profiles: while the R. damascena oil showed a balance between the eleoptene and stearoptene fractions of the oil (the average ratio between the main terpene alcohols and paraffins was 2.65), in the Kushui and R. rugosa oils, the odorous liquid phase strongly dominated over the stearopten, with a ratio of 16.91 and 41.43, respectively. The most abundant terpene was citronellol, ranging from 36.69% in R. damascena to 48.32% in R. rugosa oil. In addition, the citronellol enantiomers distribution, which is an important marker for rose oil authenticity, was studied for the first time in R. rugosa oil.

GT Transcription Factors of Rosa rugosa Thunb. Involved in Salt Stress Response.

Rosa rugosa was a famous aromatic plant while poor salt tolerance of commercial cultivars has hindered its culture in saline-alkali soil. In many plants, the roles of GT (or trihelix) genes in salt stresses responses have been emerging. In the wild R. rugosa, a total of 37 GTs (RrGTs) were grouped into GT-1, GT-2, GTγ, SH4, and SIP1 lineages. SIP1 lineage expanded by transposition. The motifs involved in the binding of GT cis-elements were conserved. Four RrGTs (RrGT11/14/16/18) significantly differentially expressed in roots or leaves under salt stress. The responsive patterns within 8 h NaCl treatment indicated that RrGTγ-4 (RrGT18) and RrGT-1 (RrGT16) were significantly induced by salt in roots of R. rugosa. Subcellular localizations of RrSIP1 (RrGT11) and RrGTγ-4 were on chloroplasts while RrGT-1 and RrSIP2 (RrGT14) located on cell nucleus. Regulation of ion transport could be the most important role of RrSIPs and RrGTγ-4. And RrGT-1 could be a halophytic gene with higher transcription abundance than glycophytic GT-1. These results provide key clue for further investigations of roles of RrGTs in salt stress response and would be helpful in the understanding the salt tolerance regulation mechanism of R. rugosa.

Novel polysaccharide and polysaccharide-peptide conjugate from Rosa rugosa Thunb. pseudofruit – Structural characterisation and nutraceutical potential

A novel bioactive polysaccharopeptide (C1) and polysaccharide (C2) with an average molecular weight of 180 kDa and 70 kDa were isolated from R. rugosa pseudofruit. The composition of the macromolecules was established using 1H NMR, FT-IR, GC–MS, SDS-PAGE coupled with enzymatic cleavage, and proteomic analyses (LC-MS). C1 was found to contain 60.56 ± 1.82 % of sugars and 21.17 ± 0.47 % of uronic acids. Its main neutral monosaccharides were arabinose, rhamnose, galactose, glucose, fucose, and mannose. C1 was found to be a polysaccharopeptide containing pectinesterase-like protein. C2 was composed of 32.85 ± 0.97 % of sugars and 48.77 ± 1.15 % of uronic acids. Its main neutral monosaccharides were galactose, glucose, rhamnose, arabinose, and mannose.A promising nutraceutical value of the polysaccharides was revealed. Assays showed strong α-glucosidase inhibitory activity of both macromolecules and considerable antiradical potential and moderate lipoxygenase inhibitory activity of the crude polysaccharide. Moreover, antiproliferative activity of C2 was observed.

Systematic Identification and Analysis of OSC Gene Family of Rosa rugosa Thunb.

The oxidosqualene cyclase family of Rosa&amp;nbsp;rugosa (RrOSC) provides a starting point for the triterpenoid pathway, which contributes to the medicinal value of the extraction of tissues of Rosa&amp;nbsp;rugosa. However, the structure and function of key RrOSCs of active triterpenoids remain ambiguous. In this study, a total of 18 RrOSC members with conservative gene structures and motifs were identified based on the genome of Rosa&amp;nbsp;rugosa. The RrOSCs were located on three chromosomes including two gene clusters that derived from gene replication. The phylogenetic relationship divided RrOSCs into six groups, and the RrOSCs of GI and GIV that were represented by lupeol or α-amyrin were identified as likely to include candidate genes for producing active triterpenoids. Considering the high expression or specific-tissue expression of the candidates, RrOSC1, RrOSC10, RrOSC12, and RrOSC18 were considered the key genes. RrOSC12 was identified in vitro as lupeol synthase. The results provided fundamental information and candidate genes for further illustration of the triterpenoid pathway involved in the pharmacological activities of Rosa&amp;nbsp;rugosa.

Crude Polysaccharide Fraction from Rosa rugosa Thunb. Root—Chemical Characterisation, Enzyme Inhibitory, Antioxidant and Antiproliferative Activity

Rosa rugosa Thunb. (Rosaceae) plantations can provide industrial amounts of roots, which contain many biologically active metabolites. Here, we report the first studies of the chemical composition and pharmacological potential of a crude polysaccharide fraction obtained from R. rugosa roots (CPL-Rx). It was found that the roots contained water-soluble and -insoluble sugars, including a large amount of β-glucans (12.95 ± 0.30 g/100 g). The water-soluble fraction was found to be mostly composed of sugars (28.94 ± 0.01%), uronic acids (2.61 ± 0.41%), and polyphenols (4.55 ± 0.12%). High-performance capillary electrophoresis analysis showed that glucose was the prevalent monosaccharide (64.31%), followed by a smaller amount of galactose (11.77%), fructose (11.36%), and arabinose (4.27%). SDS-PAGE followed by liquid chromatography mass spectrometry revealed the presence of unbound protein identified as protein plastid transcriptionally active 14-Like. CPL-Rx was found to inhibit the activity of pro-inflammatory enzymes (cyclooxygenase and hyaluronidase) and to have antioxidant potential in the Trolox equivalent antioxidant capacity assay (0.302 ± 0.01 mmol Trolox/g), DPPH radical scavenging effect (EC50 3.92 ± 0.12 mg/mg DPPH•) and oxygen radical absorbance capacity assay (0.134 ± 0.10 mmol Trolox/g). It also exerted a slight antiproliferative effect on SW480 (colon) and A549 (lung) cancer cell lines. Our research provides the first insights into the composition and pharmacological application of crude polysaccharides from rugosa rose roots. It suggests that CPL-Rx may potentially be used for cosmetic and bio-medical purposes, especially as an antihyaluronidase or chemopreventive agent.

Effect of Petal Color, Water Status, and Extraction Method on Qualitative Characteristics of Rosa rugosa Liqueur.

Flowers of Rosa rugosa Thunb. are a rich source of bioactive compounds with high antioxidant properties and are used for the production of jams, teas, juices, and wines. In the present paper, the petals of R. rugosa cv. Alba (white flowers) and Rubra (purple flowers) were evaluated for their morphological and phytochemical characteristics, and for the preparation of an alcoholic liqueur. In particular, the effect of two extraction procedures (conventional and maceration) of fresh and dry petals on the quality of a rose liqueur was determined. As expected, the concentration of the flower’s bioactive compounds was affected by petal water content and by tested cultivars: dry petals showed higher total carotenoids and anthocyanins contents with respect to the fresh ones; cv. Rubra evidenced higher values compared to cv. Alba. As regards the quality of rose liqueur, the two petal extraction procedures did not affect the polyphenol content and higher values were recorded only on dry petals with respect to the fresh ones and, in particular, on those from cv. Rubra. The liqueur’s flavonoid content was influenced by the petal extraction method, water content, and color as higher values were recorded on rose liqueur prepared after the maceration of cv. Rubra dry petals whereas lower values were observed on alcoholic drinks prepared after the conventional extraction of cv. Alba fresh petals. Our study shows that R. rugosa petals have a fair amount of secondary metabolites with antioxidant activity, making them suitable for use in the beverage industry.

Rosa rugosa polysaccharide induces autophagy-mediated apoptosis in human cervical cancer cells via the PI3K/AKT/mTOR pathway

The aim of this study was to investigate the effect of a polysaccharide from Rosa rugosa Thunb. on human cervical cancer cells (HCCCs) and the underlying mechanism. Here, a novel Rosa rugosa polysaccharide, named as RRP, was purified from Rosa rugosa petals. RRP consisted of glucose, galacturonic acid, mannose, rhamnose, galactose, arabinose, xylose, and glucuronic acid (molar ratio: 7.78:7.59:4.23:3.22:3.15:1.65:1.00), with Mw of 327.92 kDa. RRP remarkably inhibited cell proliferation, migration, and cell cycle arrest in HeLa and SiHa cells. Furthermore, RRP induced apoptosis by activating the caspase family of proteins and mediating the reactive oxygen species (ROS)-mediated mitochondrial pathway. In addition, RRP was found to dose-dependently induce autophagy, which occurred prior to apoptosis. RRP also primarily induced autophagy-mediated apoptosis in HCCCs via the PI3K/AKT/mTOR pathway. Thus, RRP might serve as a legitimate therapeutic drug candidates against human cervical cancer.

Antagonistic Activity of Lactic Acid Bacteria and Rosa rugosa Thunb. Pseudo-Fruit Extracts against Staphylococcus spp. Strains

Staphylococcus bacteria are ubiquitous microorganisms. They occur in practically all environments. They also show the ability to colonize the skin and mucous membranes of humans and animals. The current trend is to look for new natural factors (e.g., plant extracts rich in polyphenols) limiting the growth of undesirable bacteria in food and cosmetics or use as an adjunct in antibiotic therapy. The aim of this study was to evaluate the effect of extracts from Rosa rugosa Thunb. on the antagonistic properties of selected lactic acid bacteria strains in relation to Staphylococcus spp. isolates. The biological material consisted of seven strains of lactic acid bacteria (LAB) and seven strains of bacteria of the Staphylococcus genus. The anti-staphylococcal properties of the Rosa rugosa Thunb. pomace extracts (the tested extracts were characterized by a high content of polyphenols, namely 8–34 g/100 g DM/dm) were tested using the well method. The conducted research showed that the pomace extracts of the pseudo-fruit (Rosa rugosa Thunb.) had the ability to inhibit the growth of Staphylococcus spp. bacteria. The minimum concentration of polyphenols inhibiting the growth of staphylococci was in the range of 0.156–0.625 mg/mL. The conducted research showed that combined lactic acid bacteria and extracts from the pomace from the pseudo-fruit Rosa rugosa Thunb. (LR systems) may be factors limiting the growth of Staphylococcus spp. bacteria. As a result of the research, two-component antagonist systems consisting of LAB cultures and extracts from Rosa rugosa Thunb. pomace were developed, which effectively limited the growth of the test strains of Staphylococcus spp. In 41% of all tested cases, the zone of inhibition of growth of bacteria of the genus Staphylococcus spp. after the use of two-component antagonist systems was higher than that as a result of the control culture (without the addition of extracts).

Cool-Warm Temperature Stratification and Simulated Bird Digestion Optimize Removal of Dormancy in Rosa rugosa Seeds.

Rosa rugosa Thunb. has been explored multi-function in medicinal, edible, cosmetic, ornamental and ecological etc. However, R. rugosa natural populations have recently declined substantially in China, besides of global climate change, this species also has the defect of limiting the reproduction of itself such as the hard-to-release seed dormancy. In this study, only 30% of R. rugosa seeds were viable, and the others were incompletely developed or diseased seeds. Without stratification, morphologically complete viable seeds imbibed water but those seeds could not germinate even after seed husk removal under suitable condition to exhibit a physiological dormancy. After cold (4°C) and warm (18 ± 2°C) stratification, macromolecular substances containing carbon or nitrogen accumulated, and respiration, antioxidant enzyme activity, and gibberellin (GA3) /abscisic acid (ABA) and auxin (IAA)/ABA ratios increased significantly in seeds. Water absorption also increased as endocarps softened. Thus, physiological dormancy of seed was broken. Although warm and cold stratification increased separation between endocarp and embryo, the endocarp binding force was removed insufficiently, because only 10.20% of seeds germinated. Therefore, stratified seeds were treated with simulated bird digestion. Then, folds and cracks in loosened endocarps increased permeability, and water absorption rate increased to 64.43% compare to 21.14% in cold and warm stratification treatment. With simulated digestion, 24.20% of radicles broke through the endocarp with plumules and cambiums to develop into seedlings. Thus, the seed dormancy type of R. rugosa is physiological as seeds imbibed water and possessed fully developed embryos with a low growth potential in combination with a mechanical constraint from the endocarp. Cold stratification helped remove physiological dormancy, and additional warm stratification accelerated the process. The optimal stratification treatment was 4°C for 45 days followed by 18 ± 2°C for 15 days. After warm and cold stratification, simulated bird digestion broke the mechanical constraint from the seed covering layers. Based on this research, production of R. rugosa seedlings can be greatly increased to help protect the species from further declines.

Growth and development of representatives of the genus Rosa L. in the Stavropol Botanical Garden

Relevance. To identify the features of the growth and development of species of roses of different geographical origin, to establish the biological minimum temperatures necessary for the beginning of flowering of representatives of the genus Rosa L., to study the morphological indicators of fruits.Methodology. The research material was 12 wild roses belonging to 4 sections: Canina Crep, Cinnamomeae DC, Luteae Crep, Pimpinellifoliae DC. The research was carried out using the methods of the Main Botanical Garden, floral and geographical analyses — according to Hrzhanovsky. A caliper was used to measure the length and diameter of 10 fruits. For the analysis of statistical data, the MS Excel table processor and the integrated MATLAB mathematical package were used.Results. The article presents the results of a study of species of roses in the Stavropol Botanical Garden of various geographical origin. The study involved 12 species belonging to 4 sections. The features of growth and development of plants in new soil and climatic conditions are revealed. The dates of the beginning of the main phenological phases andtheir duration have been established: spring regrowth of shoots, budding, beginning and end of flowering, fruiting. In the studied species of roses, the growing season begins in the first — second decade of March, flowering occurs in late May — early June, the fruits ripen in August — September. The dependence of the timing of the beginning of flowering in the studied roses on the dynamics of the accumulated average daily air temperatures and the sum of effective temperatures above 5 °C was revealed. Mathematically, it was proved that the lower limit of the air temperature required for the beginning of flowering of species of roses is +16 °C, when the sum of the average daily temperature air St° = 1007.75–1069.48 °С and St эф &gt; 5 °С = 530.75–594.75 °С are accumulated. Early flowering species of roses include Rosa foetida Herrm., R. pendulina L., R. pimpinellifolia L., late flowering — R. caudata Baker and R. corymbifera Borkh. The shortest flowering period is in R. caudata Baker, R. jacutica Juz. and R. kamtschatica Vent. Representatives of those types mostly bloom once. Their flowers are formed on biennial and more mature shoots. The flowering period is 13–23 days. Rosa rugosa Thunb. blooms again, its flowering period is extended and long — from 40 to 48 days. All species of roses form fruits. The longest fruits are in R. pendulina L., R. caudata Baker, R. rugosa Thunb., R. corymbifera Borkh. and R. canina L., and large ones — in R. rugosa Thunb., R. marretii Lev. and R. roxburghi Tratt.