Ruellia Simplex Research Articles

Assessment of Ornamental Plant <i>Ruellia simplex</i> and Bio-Adsorbent for Removal of Amoxicillin and Ciprofloxacin – A Preliminary Test for Constructed Wetlands

Assessment of Ornamental Plant <i>Ruellia simplex</i> and Bio-Adsorbent for Removal of Amoxicillin and Ciprofloxacin – A Preliminary Test for Constructed Wetlands

In-vivo toxicological study of cysteamine modified carbon dots derived from Ruellia simplex on fruit fly for potential bioimaging

Modern medicine must shift its paradigm from conventional treatment strategies to theranostics to meet individuals' needs. Carbon dots, as a class of fluorescent materials, provide biocompatible and multifunctional solutions for a wide range of applications including clinical sensing, imaging, and drug delivery. Various studies have focused on their synthesis, photophysical properties, and innoxious nature but their potential biomedical applications for the in-vivo toxicity are yet limited. In this work, previously synthesized C-Dots (CDs) were utilized to determine their effect on the development of Drosophila melanogaster. Simultaneously, the genotoxic potential of CDs was evaluated on specific larval cell types that play important roles in immunological defence as well as growth and development. The gut organ toxicity of both CDs was studied using DAPI and DCFH-DA dyes wherein RS-CDs didn't show significant toxicity to the concentration 500 μg/mL whereas RS-Cys-CDs showed nuclear fragmentation and modest ROS (reactive oxygen species) production. Subsequently, trypan-blue assay, larvae crawling assay, touch sensitivity, adult phenotype, and survivability assay were performed. The trypan-blue assay shows the non-toxic nature of both CDs even at the concentration of 500 μg/mL. The high concentrations of RS-Cys-CD (500 μg/mL) were further associated with the alteration in touch behaviour and decrease in pupa hatching. In-vivo and ex-vivo fluorescence assessment of both the CDs exhibit bright fluorescence in green and red channels upon excitation at 485 and 577 nm respectively. The prominent imaging results from RS-Cys-CDs highlight the positive impact of surface modification.

Analysis of Practical Design for Making Natural Acid-Base Indicator Paper from Purple Golden Flower Extract (Ruellia simplex) as Teaching Material for Acid-Base Material

Experiments are an essential part of chemistry. An indicator in the form of a synthetic indicator is generally used in acid-base practice. Using synthetic indicators can cause waste that is dangerous for the environment and is relatively expensive. Purple golden flowers are one of the plants that contain high levels of anthocyanin compounds, which have the potential to be an alternative in making natural acid-base indicators. This research aims to determine the curriculum and learning outcomes of acid-base material, the potential of natural indicator paper for purple golden flowers as a substitute for synthetic indicators, and the feasibility of indicator paper as a design for chemical experiments in SMA/MA. This research uses descriptive qualitative methods, which include literature studies, interviews, observations, and experiments. The purple golden flower indicator is produced by extraction using the maceration method using 96% ethanol solvent. The research results show that paper from purple golden flower extract can be used as a natural acid-base indicator by producing a pink color change in acidic solutions and a green color in essential solutions. Based on the natural indicator paper analysis, the purple golden flower can be used as an alternative indicator in practicum design in schools

Ornamental Invasive Plants in Florida with Research-founded Alternatives

The ornamental horticulture industry has long been significant in its vast economic contributions to the US agricultural sector, with Florida ranking second in nursery and greenhouse plant sales. A small proportion of introduced plants eventually escape cultivation and become invasive, leaving fragile ecosystems at risk. In response, a series of propagation and production research studies have been conducted over the years to 1) evaluate the female sterility and landscape performance of cultivars and/or hybrids of ornamental invasives, and 2) develop reliable propagation systems of novel or underused natives having ornamental and ecological value. Attractive, fruitless selections of popular species such as butterfly bush (Buddleja sp.), heavenly bamboo (Nandina domestica), Mexican petunia (Ruellia simplex), lantana (Lantana strigocamara), trailing lantana (Lantana montevidensis), privet (Ligustrum sp.), maiden silvergrass (Miscanthus sp.), and fountain grass (Pennisetum sp.) have been identified as suitable non-native alternatives to the invasive or potentially invasive resident species (wild type). Simultaneously, researchers have taken a closer look at native plant alternatives that may offer similar aesthetic traits as invasive plants, while bringing added biodiversity and function for more ecologically friendly landscapes and gardens. As such, successful multisite trialing and/or propagation systems have been developed for a number of species native to Florida, such as squareflower (Paronychia erecta), coastalplain honeycombhead (Balduina angustifolia), wireweeds (Polygonella sp.), blue porterweed (Stachytarpheta jamaicensis), wild coffees (Psychotria sp.), sweet acacia (Vachellia farnesiana), and wild lime (Zanthoxylum fagara). With pronounced marketing and consumer education, it is hopeful that together sterile cultivars and native species will ultimately replace wild-type forms of commercially available ornamental invasives. This paper summarizes the current status of ornamental invasives in Florida and the role of native species and sterile non-native cultivars.

Ruellia simplex C. Wright (Acanthaceae): Antinociceptive, anti-inflammatory, and antidiabetic activities of a novel fatty acid isolated from its leaf extract

Ruellia simplex is a medicinal plant whose leaf is used to treat pains, inflammation, and diabetes in Nigeria. The current study was undertaken to determine the antinociceptive (analgesics), anti-inflammatory, and antidiabetic activities of a novel fatty acid isolated from the leaf extract of R. simplex. Isolation of a novel fatty acid from the most active fraction was carried out on silica gel column chromatography while, antinociceptive, anti-inflammatory, and antidiabetic activities of the isolated compound were evaluated by acetic acid, carrageenan, and alloxan-induced animal models respectively. The chemical structure of the new compound was elucidated by FT-IR, NMR, GC-MS, and LC-MS. The isolated fatty acid showed inhibition of pains by decreasing abdominal writhing in mice in dose dependent fashion as well as reduced paw volume in the carrageenan-induced paw edema in rats at IC50 = 12.5 ± 1.08 μg/ml and 10.21 ± 1.02 μg/ml, respectively, whereas the antidiabetic activity showed a dose dependent reduction in blood sugar levels with IC50 = 6.02 ± 0.01 μg/ml. The compound showed the following features: R-COOH functional group at 3327 wavelength cm-1 by FTIR; EI-MS [M]+* at m/z 467, peak area 62.231% and RT 14.086 min by GC-MS; singly charged fragments at m/z 116.1 and m/z 465.1, RT 1.31 min by LC-MS and eight proton signals consisting of singlets and multiplets (1H), thirty carbon atoms (13C) NMR data. From the study, the novel fatty acid from R. simplex extract was potentially active for the treatment of pains, inflammation, and diabetes.

Response of Ruellia simplex plants grown under water intervals and anti-transpirant treatments

Ruellia is herbaceous perennials are described as colorful groundcovers and it is very popular landscape plant cuttings of Ruellia plant were planted in pots. In the National Research Centre's greenhouse in Dokki, Giza, Egypt. A pot experiment was conducted in July 2020 and 2022 to examine the influence of KCl spraying and duration of irrigation on Ruellia plant growth and chemical contents. The current study compares the growth parameters of plants treated with KCl in three sprays at concentrations (0, 50 and 100 ppm) and water regime (3, 6 and 9 days) to those of control plants. However, increasing irrigation intervals for Ruellia plants there had no significant on growth parameters (plant height, branches number, root length, stem diameter and fresh and dry weight of shoots), while increasing irrigation intervals from 3days up to 9 days, increased flower number and delayed flowering. In addition, it is evident that N% was the highest by increasing irrigation intervals up to 9 days while P% showed a constant content, however the lower N% was recorded with the low irrigation intervals. Carbohydrate % as well as protein and photosynthetic pigments saved the same trend percentage. It can be concluded that, the increasing KCl concentration level up to 100 ppm caused an increase in previous mentioned vegetative growth parameters, flower number, flowering date, and chemical constituents (total chlorophyll, carbohydrate, protein%, carotenoids and mineral elements (N, P and K) concentrations compared with control and other KCl treatments. The purpose of this study is to learn more about the effects of KCl and water intervals on plant development and chemical components.

Biochar alleviating heavy metals phytotoxicity in sludge-amended soil varies with plant adaptability

Recycling sewage sludge (SS) to soil potentially causes soil heavy metal (HM) pollution and plant phytotoxicity. Biochar plays an important role in alleviating HM phytotoxicity, and responses vary with the feedstocks and usage of biochar. However, the effect of plant adaptability on biochar-mediated alleviation is poorly understood. Here, SS-derived biochar (SB) and rice straw-derived biochar (RB) applied at rates of 1.5% and 3% (W/W, SB1.5, SB3, RB1.5, and RB3) were used to improve the properties of soil amended with SS at 50% (W/W). Alleviation of phytotoxicity by biochar was further analyzed with SS-sensitive plant Monstera deliciosa and SS-resistant plant Ruellia simplex. Results revealed that both SB and RB significantly decreased the soil's bulk density and increased water retention. They also changed soil organic matter content and HMs fractionation. The addition of SB or RB alleviated the SS phytotoxicity, and they significantly promoted the growth and the root morphology and physiological index of M. deliciosa. But for R. simplex, these significant changes only synchronously occurred in SB3 treatment. The alleviation in M. deliciosa was more prominent and more closely connected with soil property changes than in R. simplex. Also, more soil property predictors were observed to play an important role in M. deliciosa growth than in R. simplex growth. These results indicated that biochar alleviating HMs phytotoxicity in SS-amended soil is associated with the changes of soil property. Moreover, the alleviation varies more prominently with plant adaptability than with biochar feedstocks and usage.

ALIEN FLORA OF SUMATRA I: TEN NEW RECORDS

The diversity of plants in Sumatra can be considered poorly known for the native species and the alien or non-native species. Recently, some new records of alien plant species have been added to the flora of Sumatra. Explorations were conducted to look for another undocumented alien plant species in this island in Langsa City, Aceh Province and Ogan Komering Ulu Regency, Sumatra Selatan Province. The results showed ten additional records of alien plants for Sumatra: Acalypha herzogiana (Euphorbiaceae), Coccinia grandis (Cucurbitaceae), Coleus monostachyus (Lamiaceae), Crossandra infundibuliformis (Acanthaceae), Ficus natalensis subsp. leprieurii (Moraceae), Melaleuca linariifolia (Myrtaceae), Ochna thomasiana (Ochnaceae), Ruellia simplex (Acanthaceae), Solanum diphyllum (Solanaceae), and Wrightia antidysenterica (Apocynaceae). Two of them (C. monostachyus and S. diphyllum) are already naturalized while others are in cultivation, or a few of them relicts of it. All species are listed, discussed, and provided with photographs to aid identification.

Assessment of Heavy Metal and Pesticide Contamination in Banana Fields and Development of Phytoremediation System in Kozhikode District, Kerala, India

Analysis of soil samples collected from various banana fields in Kozhikode district was carried out to identify the extent of heavy metal and pesticide contamination. Analysis by atomic absorption spectrometry revealed contamination of lead in some of the banana fields (Omasseri, Balussery, Vengeri & Nanmanda) in the district. The Gas Chromatogram analysis revealed that the banana fields in Kozhikode district were free of organo-chloride and organo-phosphate pesticide contamination. Among the several ornamental plants evaluated for assessing lead phytoremediation potential, Dianthus chinensis L. showed the highest phytoaccumulation potential. Chrysanthemum indicum L., Ruellia simplex C. Wright and Lantana camara L.also showed high lead accumulation. Therefore, these ornamental plants could be used in phytoremediation to remove lead from contaminated soils.

The complete chloroplast genome of ornamental plant Ruellia simplex C.Wright (Acanthaceae)

Ruellia simplex C.Wright is a perennial plant of the Acanthaceae, which has significant ornamental value. Because of its strong adaptability, it is widely planted in Chinese rural areas. Based on sequencing data from Illumina, the first complete chloroplast (cp) genome of Ruellia simplex C.Wright is reported in this paper. This cp genome was 143,016bp in length, including a large single-copy region (LSC) of 91,857bp, a small single-copy (SSC) of 17,591bp and two inverted repeat regions (IRs) of 16,784bp. It contained 128 genes, 35 transfer RNA genes, 8 ribosomal RNA genes, with an overall GC content of 38.41%. Additionally, the phylogenetic analysis showed that Ruellia simplex is closely related to Strobilanthes cusia (NC_037485), Strobilanthes bantonensis (MT576695) and Echinacanthus attenuatus (NC_039762). The results of this study provide valuable information for the continued study of its species evolution, genetic engineering and germplasm resource utilization.

Vegetation diversity and structure of urban parks in Cilegon City, Indonesia, and local residents’ perception of its function

Abstract. Muhlisin, Iskandar J, Gunawan B, Cahyandito MF. 2021. Vegetation diversity and structure of urban parks in Cilegon City, Indonesia, and local residents’ perception of its function. Biodiversitas 22: 2589-2603. The existence of urban parks is very essential for cities because they provide ecosystem services and benefits to humans. Diversity and structure of vegetation in urban parks play significant role in delivering several functions of ecosystem services. This paper aims to explore and analyze the vegetation diversity and structure, as well as ecosystem services of eight urban parks in Cilegon City, Banten Province, Indonesia. Vegetation analysis and residents’ perception are also conducted in this research. The analysis of vegetation is focused on horizontal structure of vegetation, including plant diversity, plant habitus, the original distribution of the plants, and vertical structure of vegetation (canopy stratification and vegetation profile). The residents’ perceptions are collected using interviews of selected respondents. The identification of vegetation recorded 114 plant species from 46 families are found in the concerned urban parks, however, the diversity and species richness in these parks tended to be low. The vegetation communities are uncertain and dominated primarily by non-native and invasive species. The canopy stratification is relatively homogeneous with the dominant plants having less than one meter in height, such as Ruellia simplex and Syzygium paniculatum. The vegetation planted in the urban parks are mostly ornamental plants that provide much more aesthetic function rather than ecological purpose, daily human needs, or other ecosystem services. This fact shows that urban parks in the study area have only few ecosystem services, hence they need some improvements in order to have more environmental services and to be able to enjoy more by the community.

Environmental conditions and the performance of free water surface flow constructed wetland: a multivariate statistical approach

During the past three decades, constructed wetlands have become an integral part of the suite of technologies for removing domestic and industrial wastewater contaminants. The use of constructed wetlands has disproportionately focused on domestic and agricultural wastewaters and storm water runoff and less on oil and gas-related produced water. In this context, the cumulative effect of environmental factors on the treatment/removal efficiency of contaminants in produced water is underserved by research. Therefore, this study assessed the effect of environmental factors (temperature, dissolved oxygen, oxidation–reduction potential, and pH) on contaminant removal efficiency in free water surface flow constructed wetland (FWSFCW) using ordinary least squares regression and experimental data from a waste treatment facility in Ghana. The results showed that environmental factors did not systematically vary across the experimental group and control set-up. Generally, the environmental factors explained relatively far less of the variance in contaminant removal efficiency compared with the plant species (Typha latifolia, Ruellia simplex and Alternanthera philoxeroides). Environmental factors cumulatively explained only 1.3%, 16.4%, 22.6%, and 5.6% of the variance in removal efficiency of BOD, COD, oil and grease, and total coliform bacteria, respectively. Temperature was the most important environmental predictor of the removal of BOD and phosphorus whereas DO was most important for removing nitrates and total coliform bacteria. ORP and pH were the most important predictors of COD, and oil and grease, respectively. These findings underscore the complex relationships among environmental factors and contaminant removal efficiency and the need for contaminant management practices and remedial techniques that address these complexities.

Substratos alternativos para produção de mudas de Ruellia simplex por estaquia

To produce quality seedlings using the vegetative propagation method, in addition to determining the ideal conditions for plant development, it is necessary to choose a substrate that has a good relationship between water storage and aeration. In this context, the objective was to evaluate the effect of different proportions of carbonized rice husk (CAC) and babassu decomposed stem (CDB) on the composition of substrates for Ruellia simplex vegetative propagation. The experiment was conducted at the Center for Agricultural and Environmental Sciences (CCAA) at the Federal University of Maranhao (UFMA). A completely randomized design with seven treatments was used, of which T1 - 100% Solo; T2 - 20% CAC + 80% Solo; T3 - 60% CAC + 40% Solo; T4 - 100% CAC; T5 - 20% CDB + 80% Solo; T6 - 60% CDB + 40% Solo; T7 - 100% CDB, with each experimental unit repeated 3 times and composed of three plants. There was no significant effect on the number of shoots and leaves, root length, root volume, fresh mass of the aerial part and root system. However, for the average length of aerial part, dry mass of aerial part and root system, a significant difference between the proportions of the substrates. We recommend the substrate containing 20% ​​babassu decomposed stem plus 80% soil and 20% carbonized rice husk plus 80% soil for vegetative propagation of Ruellia simplex.

Joint influence of hydraulic load and hydraulic retention time on oilfields wastewater contaminant removal dynamics in free water surface flow constructed wetland

Constructed wetlands have been proposed to address the frequency and magnitude of oil and gas-related environmental contamination. The effect of co-variation of hydraulic load and hydraulic retention time on the dynamics of contaminant removal efficiency of heterogeneous plant species was assessed using ordinary least squares regression. The results showed that hydraulic load (HL), hydraulic retention time (HRT) and plant species jointly explain 87%, 79%, 83%, 85% and 66% of the total variance in removal efficiency of conductivity, TDS, BOD, COD, and total coliform bacteria, respectively. The models also explain 86%, 80% and 81% of the variations in removal efficiency of oil and grease, total phosphorus, and nitrate. More than 90% of the explained variance of total coliform removal efficiency is jointly attributable to hydraulic load and retention time. Hydraulic load of 1000 L and retention time of 72 h (1000 L 72 h) recorded optimum removal efficiency for TDS and conductivity. Optimum removal efficiency for BOD, COD and total coliform bacteria were achieved at HL and HRT of 1000 L 72 h, 1000 L 48 h and 1250 L 24 h, respectively. Alternanthera philoxeroides recorded the highest removal efficiency for oil and grease, conductivity and TDS, whereas Ruellia simplex recorded the highest removal efficiency for COD. Typha latifolia had the highest removal efficiency for total phosphorus and nitrate. Plant species suppressed the relationship between HL and HRT (1250 L 48 h, 1500 L 72 h, 1750 L 48 h and 2000 L 48 h) and removal efficiency for conductivity. Similarly, plant species suppressed the relationship between 1000 L 48 h and 1750 48 h and removal efficiency for TDS. These relationships underscore the complex dynamics between optimal contaminant removal efficiency and required hydraulic load, hydraulic retention time and plant species.

Natural Area Weeds: Mexican Petunia (Ruellia simplex)

Minor Revision.
 Ruellia simplex (Mexican bluebell, Mexican petunia, or Britton's wild petunia) is an herbaceous perennial (USDA Hardiness Zone 8–11) in the family Acanthaceae. Five species of Ruellia are native to Florida, and three non-native species are listed as naturalized in the state, including R. simplex (Wunderlin et al. 2019). There has been some confusion about the name of this species, commonly referred to as Ruellia brittoniana, Ruellia coerulea, Ruellia tweediana, orRuellia malacosperma (USDA NRCS 2019; Wunderlin et al. 2019). Ruellia simplex C. Wright was the name used when this species was first described in Cuba in 1870; therefore, it has taxonomic priority (Ezcurra and Daniel 2007). This name is now widely used in the horticultural trade and amongst state agencies and will therefore be used in this publication.https://edis.ifas.ufl.edu/ep415

Ruellia simplex cuttings propagation produced on carnauba waste substrate

The production of quality seedlings is necessary for the trading of ornamental plants. Seedling quality depends on several factors, among these factors, the substrate is highlighted. Thus, this study aimed to evaluate the effect of substrates based on bagana of carnauba, in the production of Ruellia simplex seedlings, by vegetative propagation. A completely randomized design was adopted, with six treatments and four replications. The treatments (substrates) were composed of bagana of carnauba (BC) and soil, in the following proportions: BC0 - 0% BC + 100% of soil; BC20 - 20% BC + 80% of soil; BC40 - 40% BC + 60% of soil; BC60 - 60% BC + 40% of soil; BC80 - 80% BC + 20% of soil; BC100 - 100% BC + 0% of soil. The plot consisted of a plant. After 45 days of cutting, it was evaluated: number of shoots; number of leaves per shoot; diameter of the highest shoot; length of the highest shoot; root length; inflorescence number; number of flowers per inflorescence; leaf area; root volume; aerial part fresh mass; root system fresh mass; aerial part dry mass; and root system dry mass. There was a significant difference for the variables, number of leaves per shoot, diameter and length of the highest shoot, number of flowers per inflorescence, fresh and dry mass of the aerial part. Having a direct relationship between the increase in the proportion of bagana of carnauba volume and the inflorescence number, the substrate 100% of bagana of carnauba is indicated.

A simple paper-based color change label using plant extracts for Ammonia gas detection

Paper and plant extracts are materials that are cheap and easy to find everywhere, have great potential to be used as a portable, low-cost and biodegradable label for the application of chemical compounds detection. In this paper, we have explored potential methods for the label to detect ammonia gas, an indicator of meat spoilage, with the colorimetric principle. By immersing paper with plant extracts that are sensitive to ammonia, in this case, is anthocyanin from Ruellia simplex flower extract, the detection of ammonia at various concentrations can be achieved. Ammonia concentration used is 0; 0.005%; 0.025%; 0.1%, 0.25% and 0.5%. Color change images on the label when detection is scanned by a flatbed scanner. ImageJ software is used to quantify the color changes. Ammonia detection at various concentrations results in a color change from red to yellow on the paper label. Paper-based labels with discoloration that can be captured visually by the consumer’s eyes are considered as promising approaches to determine the freshness of meat.

Observation of Flower Visitors to Determine their Potential Role as Pollinators of Ixora coccinae and Ruellia simplex

This study aimed to determine the potential pollinating agents of Ixora coccinae and Ruellia simplex at Universiti Malaysia Terengganu (UMT) campus based on the foraging activities of the flower visitors. The diurnal observations (0700 hr to 1800 hr) were conducted for a total of 144 hours and 96 hours for I. coccinae, for R. simplex respectively. The flower visitor activities (landing or hovering at the flowers) and the time spent at the flowers however were recorded for only the first 10 minutes of every hour between 0700 hr and 1000 hr for four days. A total of 383 flower visitations was recorded by 17 insect species and a bird species, the olive-backed sunbird (Cinnyris jugularis). For I. coccinae, the most common visitors were Apis cerana, Heterotrigona itama and Xylocopa confusa, in which all species were found to show only landing behaviour to forage at the flowers, and the time spent foraging at the flowers was not significantly different between the species (Kruskal-Wallis test, H = 1.323, df = 2, p = 0.055). For R. simplex, the most common visitors were A. cerana with 100% landing, and C. jugularis which recorded 80% landing and 20% hovering during their visits to the flowers. Between the two, A. cerana recorded significantly longer time spent at the flowers as compared to C. jugularis (Mann-Whitney test, U = 17.355, p < 0.001). Based on the visitation rate and feeding behaviour of the flower visitors, A. cerana, H. itama and X. confusa were the potential pollinating agents for I. coccinae while for R. simplex, A. cerana showed higher potential to be a pollinating agent as compared to C. jugularis. Therefore, this study highlighted the importance of recording the behaviour of each flower visitor to determine the relative contribution to the pollination success of the plant species visited.

Production of stunted pot plants from Ruellia simplex

Production of stunted pot plants from Ruellia simplex

Studi Morfologi Stomata Daun Glodokan (Polyalthia longifolia Sonn) pada Lingkungan Berbeda

Abstract. This study investigated a natural acid-base indicator which is extracted from plants in Kupang city. There are 14 plants that potential as a source of natural indicator for acid base titration, i.e, KolUngu (Brassica oleracea Capitata Group), Turi Merah flower (Sesbania grandiflora L. Pers), BelimbingWuluh flower (Averhoa bilimbi L), Kaktus Merah fruit (Opuntia vulgaris Mill), Ruelia flower (Ruellia simplex), Flamboyan flower (Delonix regia), bugenvil flower (Bougainvillea spectabilisWilld.), Bayam Merah leaves (Amaranthus tricolor L.) Jamblang fruit (Syzygiumcumini L.), Murbey fruit (Morus alba L.), Pinang fruit (Areca catechu L.), Sirih fruit (Piper betle L.), Kunyit (Curcuma longa Linn), and Nanas Kerang leaves (Rhoeo discolor). Plants extract shows a sharp color change in acid and base solution. Promising results as a natural indicator also shown in acid base titration which is have similar equivalent point to synthetic indicator. We can use these natural indicators as an alternative to synthetic indicator because they are found to be simple, very useful, cheap, easy to extract, accurate, and eco-friendly. Keywords: plant, natural indicator, acidbase.