ABSTRACTTurbulence structures in flow over three types of wall roughness: sand-grain, cube roughness and a realistic, multi-scale turbine-blade roughness, are compared to structures observed in flow over a smooth wall in open channel flow at , using direct numerical simulations. Two-point velocity correlations, length scales, inclination angles, and velocity spectra are analysed, and the applicability of Townsend's outer layer similarity hypothesis [Townsend. The structure of turbulent shear flow. Cambridge: Cambridge University Press; 1976] to these parameters was examined. Results from linear stochastic estimation suggest that, near the wall, the quasi-streamwise vortices observed in smooth-wall flow are present in the large-scale recessed regions of multi-scale roughness, whereas they are replaced by a pair of ‘head-up, head-down’ horseshoe structures in sandgrain and cube roughness, similar to those observed by Talapatra and Katz [Coherent structures in the inner part of a rough-wall channel flow resolved using holographic PIV. J Fluid Mech. 2012;711:161–170]. The configuration of conditional eddies near the wall suggests that the kinematical process of vortices differ for each kind of rough surface. Eddies over multiscale roughness are conjectured to obey a growth mechanism similar to those over smooth walls, while around the cube roughness the head-down horse-shoe vortices of Talapatra and Katz [Coherent structures in the inner part of a rough-wall channel flow resolved using holographic PIV. J Fluid Mech. 2012;711:161–170] may undergo solid-body rotation on top of a cube roughness on account of the strong shear layer, shortening the longitudinal extent of near-wall structure and promoting turbulence production during this process. These results illustrate the sensitivity of turbulence structure to the roughness texture, particularly within the roughness sublayer.