Roselle Calyx Research Articles

Antioxidant Compounds and Activities of Roselle (Hibiscus sabdariffa L.) Decoction Residues from Cordial and Juice Production

Roselle is a popular plant in Malaysia, especially for its use in food and beverage production. The calyces of the roselle plant are commonly decocted to make roselle juice and cordial, resulting in a significant amount of waste in the form of decocted calyces. Surprisingly, these decocted calyces retain their dark red colour and are still intact although soften. However, to date, there is no research on the antioxidant compounds and activities of roselle decoction residues from cordial and juice production have been reported. Therefore, the aim of this study is to determine the phenolic compounds and antioxidant activities of the roselle decoction residues. The roselle decoction residues were evaluated for colour, Delta E (∆E), pH, total anthocyanin content (TAC), total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging assay, ferric reducing ability assay (FRAP), and ferrous ion chelating (FIC). All assays were also correlated and show positive effect among themselves. The decocted residues reported were still in acidic conditions ranged of pH 2 to 4. The decocted cordial residue (DCR) showed similar colour with control or fresh roselle calyx (FRC), and a bit duller in decocted juice residue (DJR). The DCR showed similar and slightly lower antioxidant content and activity of TAC, TPC, TFC, DPPH, FRAP and FIC to FRC but much better than DJR. Therefore, the DCR were still rich in anthocyanin contents and had good antioxidant activity without having colour changes. These residues have the capacity to be converted to wealth and could be an alternative source for natural antioxidants. Further research is needed to explore their potential applications in functional foods, dietary supplements, and pharmaceuticals. By harnessing the potential of roselle decoction residues, we can contribute to a more sustainable and environmentally friendly approach to food and beverage production.

Improved extraction of anthocyanins with antibacterial activity from fresh roselle (Hibiscus sabdariffa L.) calyces

Roselle (Hibiscus sabdariffa L.) flower is a commonly consumed vegetable in Bangladesh and have culinary value to preparation of soup, jelly, and baking. Since previous studies suggested the presence of anthocyanins in roselle calyces, this study was designed to enrich anthocyanins from the calyces of this flower. This study explored that addition of mineral acid during the extraction process further enriched the anthocyanins content of the fresh roselle calyces extract. Also, methanol acidified with 1% (v/v) HCl was found as the best solvent to extract anthocyanins from the calyces resulting 7.621±0.001 mg crude anthocyanins per gram of fresh sample. Anthocyanin extraction decreased if 1% (v/v) HCl was replaced with 10% (w/v) tartaric acid or 10% (v/v) acetic acid. The extracted anthocyanins showed minimal antibacterial activity. A further study will explore the possibility of use these anthocyanins for industrial purposes. Dhaka Univ. J. Biol. Sci. 33(2): 139-147, 2024 (July)

Study of sensory evaluation, and cost evaluation of products made from roselle calyces

Study of sensory evaluation, and cost evaluation of products made from roselle calyces

Kinetics studies on the ascorbic acid extraction from roselle (Hibiscus sabdariffa) calyces

The effect of processing conditions of roselle (Hibiscus sabdariffa) calyces on the vitamin C content of the extracts was studied to develop models that best describe the process. The extraction parameters are the processing time (5, 10, 15 mins), process temperature (30oC, 50℃, 75℃ and 100℃), and calyx-water mass ratio (1:50, 1:20 and 1:10). These samples were tested for ascorbic acid using the spectrophotometric method. Statistical analysis was conducted on the experimental results and regression models were developed. A total of six mathematical models were selected for the description of vitamin C extraction from the calyces. The results showed that time, temperature and calyx-water mass ratio have a linear significant effect (p ≤ 0.01); while time-temperature and temperature-ratio had a significant (p ≤ 0.05) interaction effect; time and temperature had a significant (p ≤ 0.05) quadratic effect on the vitamin C content of the extract. Pseudo-first-order gave the highest overall coefficient of determination R2 and lowest overall root mean square error (RMSE). The activation energy of vitamin C ranged from 3 to 8.4 kJ; the effective diffusion coefficient ranged from 1.5×10-9 to 5.0×10-7 m 2 s -1 ; while mass diffusivity ranged from 3.11×10-5 to 8.6×10-4 ms-1 . The Biot number calculated ranged between 33.93 and 541.94 which showed that there is an insignificant external mass transfer resistance.

The characteristics of foam-mat dried red and purple roselle calyces powder prepared with commercial emulsifiers as the foaming agents

The characteristics of foam-mat dried red and purple roselle calyces powder prepared with commercial emulsifiers as the foaming agents

Roselle (HibiscussabdariffaL.) calyces tea improves physical fitness of healthy adults.

Roselle calyces (Hibiscus sabdariffa L.) is a plant that contains anthocyanin and flavonoids, which function as exogenous antioxidants for the human body to counteract excess oxidative stress. Roselle has anti-hypertensive, anti-cancer and anti-diabetic effects. The present study evaluated roselle to see whether it affects physical fitness. The components of physical fitness include strength, balance, right and left hand grip, vertical jump and VO2max (maximum oxygen consumption). A total of 30 subjects received 200 ml rosella tea for 30 days every morning and evening. Every week, subjects were assessed for physical fitness. Data analysis used paired t and Wilcoxon test according to the normality test results. The results showed significant improvements in strength (from 24.9 to 27.3 kg; P=0.025), balance (from 23.3 to 42.2 sec; P=0.004), right (from 31.8 to 35.1 kg; P<0.0001) and left hand grip (from 29.8 to 31.6 kg; P=0.020), vertical jump (from 38.6 to 41.1 m/sec; P=0.008) and VO2max (from 31.1 to 34.3 ml/kg/min; P=0.014). This demonstrated that roselle significantly improved six parameters of physical fitness and may be used as a supplement to improve physical fitness without severe side effects.

Roselle Calyx (Hibiscus sabdariffa L.) Ethyl Acetate Fraction Lowering Malondialdehyde and TNF-α and Reducing Hypercoagulability in Diabetic Model

Introduction: Traditionally and empirically, Hibiscus sabdariffa L. has been used in treating diabetes mellitus due to its antioxidant activity. This study aimed to investigate the effect of administering the ethyl acetate fraction of hibiscus calyxes (EAFHCs) on malondialdehyde (MDA) levels, tumor necrosis factor-α (TNF-α) levels, bleeding time, and platelet count in male white rats induced with streptozotocin-induced diabetes. Method: Thirty-six Wistar Kyoto rats were induced with intraperitoneal streptozotocin at 55 mg/kg BW and stabilized for 5 days to obtain diabetic conditions. Diabetic animals were divided into four groups; the diabetic group was given vehicle, the glibenclamide group was given 0.45 mg/kg BW of glibenclamide, and two groups were administered the EAFHCs at doses of 100 mg/kg BW and 200 mg/kg BW for 5 days. Subsequently, the MDA, TNF-α, bleeding time and platelet count levels were examined on days 1, 3, and 5, respectively. All data were analyzed using two-way ANOVA followed by the Duncan Multiple Range Test (DMRT). Results: EAFHC significantly reduced MDA and TNF-α levels (p < 0.05). Additionally, this fraction appeared to shorten bleeding time and decrease platelet count in diabetic rats. Administration of the EAFHC for 5 days effectively lowered MDA and TNF-α levels significantly, decreased platelet counts and prolonged coagulation (p < 0.05) in diabetic rats. Conclusion: This study demonstrates that EAFHC effectively reduces MDA and TNF-α levels and reduces the risk of hypercoagulability in diabetic model.

Halochromic smart packaging film based on montmorillonite/polyvinyl alcohol-high amylose starch nanocomposite for monitoring chicken meat freshness

Polyvinyl alcohol (PVA) was blended with high amylose starch (HAS) at a ratio of 3:1, and reinforced with montmorillonite (MMT K10) at different concentrations (1, 2, 5, and 7 % w/w of polymers) and anthocyanins (ANT) to develop an active and smart packaging film. MMT addition enhanced the film's mechanical, barrier, thermal, and water resistance properties. Incorporating ANT extracted from roselle calyx into the optimal nanocomposite film (MMT/PVA-HAS II) increased the films' antioxidant, pH-response, and antibacterial properties. FTIR, XRD, and SEM confirmed the intermolecular interactions and even distribution of ANT and MMT in the film matrix. Release rate of ANT was dependent on type of simulant, with higher rate in aqueous solutions compared to alcoholic/fatty food simulants, and cytotoxicity evaluation proved safety of films for food packaging applications. Storage experiments confirmed the potential applicability of the novel halochromic smart film as a promising candidate for monitoring chicken spoilage under abusive storage conditions.

Bioaccessibility of iron and zinc content of a traditional pearl millet gruel acidulated with extracts of roselle calyces and tamarind pulp

Iron and zinc deficiencies are a public health concern in the Far-north region of Cameroon, where diets are based on cereals containing inhibitors of minerals’ absorption. This work investigated the effect of acidulating a traditional pearl millet gruel with extracts of roselle calyces and tamarind pulp on iron and zinc bioaccessibility. We first determined pH, titratable acidity, phytates and polyphenols content of decoction and maceration extracts. Phytates, polyphenols and minerals content of the acidulated gruels as well as the bioaccessibility of iron and zinc using a simulated in vitro digestion method were then assessed. Finally, we evaluated the acceptability of the acidulated gruels. The pHs of roselle calyces extracts (2.59 – 2.71) were the lowest (p < 0.05) while phytates (114.61 – 115.72 µg.mL−1) and polyphenols (100.80 – 116.32 µg.mL−1) were more abundant (p < 0.05) in tamarind pulp extracts. There were no significant differences (p ˃ 0.05) in the titratable acidity of all extracts (0.20 – 0.24 g .100 mL−1). Gruels acidulated with decoction and maceration extracts of tamarind pulp were more concentrated (p < 0.05) in phytates (469.66 mg .100 g−1 DM), polyphenols (70.91 mg .100 g−1 DM) and Iron (5.70 mg .100 g−1 DM), respectively. All the acidulated gruels exhibited higher (p < 0.05) bioaccessible minerals with the greatest increases (22.28% for iron and 61.41% for zinc) being found in decoction and maceration extracts of roselle calyces, respectively. No acidulated gruels had a general acceptability score inferior to that of the control. The acidulation of pearl millet gruels with roselle calyces and tamarind pulp extracts could be an efficient and affordable food-based strategy to fight against iron and zinc deficiencies in at-risk populations.

A comprehensive in vivo study of the antihypertensive properties and toxicity of roselle (Hibiscus sabdariffa L.)

Background: Roselle (Hibiscus sabdariffa L.) calyces have been used in traditional medicine as diuretics, mild laxatives, and antihypertensive agents but to date, a comprehensive study of its pharmacological activity and safety has not been conducted. Aims of the study: The current study aims to provide a comprehensive evaluation of the antihypertensive efficacy and toxicity profile of Roselle (H. sabdariffa L.) calyces extract. Utilizing animal models, the investigation assessed the dose-dependent pharmacological effects and safety of H. sabdariffa L. Results: The findings indicate that the extract exerts a significant antihypertensive effect at a dose of 250 mg/kg body weight (BW), lowering systolic and diastolic blood pressures by 10.12% and 11.63%, respectively. Ethyl acetate fractions administered at 112.5 mg/kg BW demonstrated greater efficacy than n-hexane and aqueous fractions, suggesting that the active compounds likely possess semi-polar properties. Acute toxicity testing yielded an LD50 of 8.75 g/kg BW for male rats and 7.5 g/kg BW for female rats, classifying the extract as slightly toxic. The sub-chronic toxicity study shows that H. sabdariffa L. demonstrates an effect on bodyweight and urea levels in male and female rats, while the change in the blood parameters, creatinine level, and the liver index was only observed in female rats. Conclusions: These data suggest that H. sabdariffa L. extract exhibits therapeutic promise but should be administered cautiously, preferably at doses lower than 250 mg/kg BW, due to potential toxicity.

Comprehensive characterization of the overlooked residue generated during roselle calyxes brewing with potential use as functional ingredient

A significant quantity Hibiscus sabdariffa L. calyxes is generated as a by-product during the decoction process commonly used for roselle beverage preparation. We conducted an extensive characterization of polyphenols, organic acids, and antioxidant potential in roselle calyxes, decoction, and their by-product. Roselle calyxes were found to be a rich source of diverse polyphenols, including delphinidin sambubioside, caffeoylquinic acids, hibiscus acid, and citric acid as major components. Importantly, we extracted a significant proportion of these bioactive compounds during the decoction process, resulting in a polyphenol-rich beverage. The used calyces (decoction by-product) retained from 23 % to 140 % of the extractable polyphenols and organic acids found in roselle calyxes. Additionally, due to the leaching of hydrophobic components like soluble dietary fiber and extractable polyphenols and organic acids, the roselle by-product was enriched with non-extractable constituents attached to dietary fiber (126 % - 272 %). Therefore, roselle calyxes and their decoction by-products emerge as promising sources of polyphenols with the potential for use in dietary supplements, alongside the commonly consumed roselle decoction.

Effects of different drying methods on the proximate composition, antioxidant activity, and phytochemical content of Hibiscus sabdariffa L. Calyx

The study investigated the impact of freeze drying (FD), cabinet drying (CD), and sun drying (SD) on the proximate composition, phytochemicals, and antioxidant activity of roselle calyx. The proximate composition, phytochemicals, and antioxidant activity of the roselle calyx were determined by the AOAC method and the free radical-scavenging DPPH technique, followed by the spectrophotometric method, respectively. The samples were frozen and condensed at -40 to -60 °C in the FD and then dried at 20 °C for 20–30 h, and in the CD and SD, it was done at approximately 65 °C and 30 °C, respectively. CD retained the least moisture (p < 0.05) and had the highest (p < 0.05) carbohydrate content (38–49 %). There was a substantial difference (p < 0.05) in the ash, protein, and fat content of the provided samples using different drying processes. The TPC of FD, CD, and SD of roselle calyx had 64.70 ± 1.33, 32.23 ± 1.36, and 25.84 ± 1.85 mg GAE/g, respectively. The TFC of FD, CD, and SD dried roselle calyx was 65.41 ± 2.02, 41.64 ± 3.12 and 27.60 ± 1.83 mg QE/g, respectively. The phytochemicals and antioxidant activity of roselle calyces were found to be significantly higher (p < 0.05) in FD compared to CD and SD. Finally, FD should be considered an appropriate drying procedure for retaining excellent qualities.

Scaling up stirring-assisted extraction and transformation of roselle anthocyanins into dried powder using spray-drying and oven-drying

Roselle (Hibiscus sabdariffa L.) is a source of anthocyanins with double functions as natural colorants and pharmacologically active ingredients in the food and pharmaceutical industries. Optimizing its extraction by upscaling and engineering the derived extract into its concentrated form or dry powder are thus needed. This study optimized the conditions of stirring-assisted extraction (SAE) of roselle calyces at an upscaled volume, determined the physicochemical and antioxidant properties of extract concentrated with rotary evaporation, and evaluated the effects of spray-drying and oven-drying on the powdered extract characterized from both properties. Results show that anthocyanin extraction from roselle was successfully upscaled using SAE with these optimized conditions: 50 % ethanol acidified with 2 % citric acid as the solvent, an S/L ratio of 1:15 g/mL, and three extraction cycles with a duration of 60 min each. Concentrated extracts (moisture content = 1.38–2.35 %) can be used as an alternative form of storage. Spray-drying with the matrix maltodextrin proved effective in producing dry powder with superior properties than oven-drying. Spray-drying and a 1:1 extract-to-maltodextrin ratio created more favorable dry powder: smooth surface, light red color, amorphous structure, spherical morphology, homogenous particle size (avg. 1.951 µm), total anthocyanin of 2.15 mg/g, antioxidant activity at IC50 = 203.56 µg/mL, and color stability maintained at pH = 2.6–4.0. These conditions enable extraction scale-ups for industrial production. Further, storage in thick concentrations and oven-drying can alternatives in specific cases when spray-drying is not doable.

Nutritional quality and health benefits of roselle calyces

Abstract Roselle calyces (Hibiscus sabdariffa L.) were evaluated through a critical study of existing research works on health benefits, mineral compositions, bioactive compositions, mechanisms, and possible research gaps. The use of roselle calyces as an alternative to synthetic food dyes, addressing growing global challenges of overweight, obesity, and cardiovascular diseases, was evaluated and encouraged. Studies indicate the attenuation of obesity by chlorogenic acid (the predominant phenolic compound in roselle calyx) via mechanisms associated with the UCP-1 and PGC-1α pathways, resulting in reduced blood lipid levels, reduced fat accumulation in the liver, and increased thermogenesis through fat metabolism. Minimum inhibitory concentration (MIC) of known bacteria and fungi, such as Listeria monocytogenes, Escherichia coli, Bacillus cereus, Salmonella typhimurium, Candida tropicalis, and Candida krusei, were studied. More research, however, needs to be conducted on organic acids present in roselle calyces to look into their possible applications and maximize their possible benefits.

Integrated Action of Rhizobacteria with Aloe vera and Moringa Leaf Extracts Improves Defense Mechanisms in Hibiscus sabdariffa L. Cultivated in Saline Soil.

Osmotic stress is a serious physiological disorder that affects water movement within the cell membranes. Osmotic stress adversely affects agricultural production and sustainability and is largely caused by soil salinity and water stress. An integrated nitrogen-fixing bacteria (NFB) soil amendment and an exogenous foliar application of Aloe vera leaf extract (ALE), and moringa leaf extract (MLE) were evaluated on roselle (Hibiscus sabdariffa L.) growth, calyx yield, secondary metabolites, and tolerance to osmotic stress in salt-affected soil. The osmotic stress markedly decreased above- and below-ground development of the roselle plant, but integrated NFB soil amendment with ALE or MLE foliar application significantly alleviated its negative impacts. Broadly, an improvement was observed in chlorophyll, carbohydrates, and protein levels following NFB and extracts foliar application, as well as a significant enhancement in antioxidant production (total phenols, ascorbic acid, and FRAP), which decreased peroxide production and increased stress tolerance in plants. Under osmotic stress, the roselle calyx revealed the highest anthocyanin levels, which declined following NFB soil amendment and foliar extract application. Additionally, an enhancement in nitrogen (N), phosphorus (P), and potassium (K) contents and the K/Na ratio, along with a depression in sodium (Na) content, was noticed. The integrated application of Azospirillum lipoferum × ALE exhibited the best results in terms of enhancing above- and below-ground growth, calyx yield, secondary metabolites, and tolerance to osmotic stress of the roselle plants cultivated in the salt-affected soil.

Exploring the Potential of Roselle Calyx and Sappan Heartwood Extracts as Natural Colorants in Poly(butylene Succinate) for Biodegradable Packaging Films.

Recently, there has been a growing concern among consumers regarding the safety of packaging products, particularly due to the presence of potentially harmful substances like synthetic pigments and inorganic dyes. These substances, which are often used to attract consumer attention, can migrate and contaminate products over extended shelf storage periods. To address this issue, the focus of this research was the development of a biodegradable packaging film using poly(butylene succinate) (PBS) incorporated with natural colorants extracted from roselle (RS) and sappan heartwood (SP). RS and SP serve as non-toxic and alternative pigments when compared to synthetic colorants. The biodegradable packaging films were prepared using blown film extrusion, encompassing different weight percentages of RS and SP (0.1%, 0.2%, and 0.3%). The films exhibited distinct colors, with RS films appearing pink to purple and SP films exhibiting an orange hue. The water vapor transmission rate slightly decreased with an increasing content of RS and SP extracts, indicating improved barrier properties. Additionally, the films showed reduced light transmittance, as evidenced by the UV-Vis light barrier results. The degree of crystallinity in the films was enhanced, as confirmed by X-ray diffraction and differential scanning calorimetry techniques. Regarding mechanical properties, the PBS/RS and PBS/SP films exhibited slight increases in tensile strength and elongation compared to neat PBS films. Moreover, the blended films demonstrated higher stability after undergoing an aging test, further highlighting their potential for use in biodegradable packaging applications. The key advantages of these films lie in their non-toxicity, biodegradability, and overall environmental friendliness.

Sustainable aquafeed development: Incorporating select fruit wastes into Zebrafish diets using mathematical model-based approach

Expensive aquafeed is a major problem in aquaculture, creating the need for a low-cost feed that provides ideal nutritional requirement to maximize growth performances. This study aims to formulate and evaluate two new optimized Zebrafish feeds (F1 and F2) using linear programming mathematical model, one of which incorporates two pigment rich fruit wastes (Pitaya peel and Roselle calyx) in the formulation. The model represents nutritional content and cost of each ingredient into linear equations, with the goal of finding ideal combination that satisfies the specific nutrient requirements. By systematically evaluating ingredient proportions, linear programming ensures that Zebrafish receives adequate nutrients at the lowest possible cost, making the feed development process more efficient and cost-effective. The novel feed formulations derived from the mathematical model were tested on the growth and pigmentation of Zebrafish in comparison to a commercial feed (control). Feed intake of F1 and F2 were generally found to be similar to the control feed, indicating the acceptability of the formulated feeds by the Zebrafish. Body weight and length of Zebrafish fed with F1 and F2 were comparable to Zebrafish fed with control feed (p > 0.05). Similarly, Zebrafish fed with F1 and F2 showed no significant differences in pigment intensity compared to Zebrafish fed with control feed (p > 0.05). The survival rate of fishes in all feeding groups were greater than 70 % with no significant differences (p > 0.05). Results obtained in this study illustrated the potential of mathematic linear programming and effectiveness of utilising pigment-rich fruit wastes in formulating an optimized economic aquafeed.

Natural spices and flavour substitution in zobo tea and drink production: A review

This paper reviews the effects of natural spices and flavour on the overall quality of zobo tea and drinks. Zobo is made from the calyces of Hibiscus sabdariffa, also known as roselle. Zobo tea and drinks are rich in nutrients (carbohydrate, fibre, ash, vitamins A and C), minerals (potassium, magnesium, sodium, calcium, iron), and bioactive compounds (organic acids, anthocyanins, flavonoids, phenolic acids) which make them predisposed to microbial proliferation, although nutrient composition and product quality vary with the variety of raw materials used and methods of production. Zobo can be consumed unsweetened or sweetened with honey, maple syrup, sugar cane, or fruit juice extracted from orange, pineapple, apple, or strawberry. Spoilage activities of microorganisms can be minimised with spices when they are used in place of chemical flavouring and preservatives. Several natural preservatives or spices of organic or natural origin could improve the quality attributes of zobo tea and drinks, and reduce both microbial diversity and density. Some of the widely utilised and potential spices include garlic, ginger, mixture of garlic and ginger, lime, clove, cinnamon, nutmeg, kola nut, and pepper. These spices, along with the dried calyces of H. sabdariffa, have antimicrobial properties, and are rich in phytonutrients, including vitamins and minerals. The overall effects are highly dependent on the biological composition of natural additives and synergistic or antagonistic effects between roselle calyces and the additives used. If properly packaged, zobo can be kept longer than zobo drinks, thus resulting in a product with longer shelf life. The use of natural additives in zobo production would go a long way towards enriching both the zobo tea and drinks, and subsequently replenishing the lack of nutrients.

Physicochemical characterization and mass modelling of Roselle (Hibiscus sabdariffa L.) calyx and seeds

Roselle (Hibiscus sabdariffa L.) calyx and seeds are underutilized, whose utilisation can be widened with the design and development of proper post-harvest systems. For this purpose, a detailed study on its engineering properties is necessary. Hence, the main goals of this study were to investigate the physical, biochemical, thermal, and textural properties of Roselle and to select the most precise models for calculating the mass from its physical properties. Roselle calyx has an average weight of 3.106 g, actual density of 1.04 g/cm3 and bulk density of 0.89 g/cm3. Similarly, Roselle seeds has an average weight of 1.427 g, actual density of 1.37 g/cm3 and bulk density of 0.65 g/cm3. Moreover both the calyx and seeds were found to be rich in protein, phenols and minerals. Finally, the FTIR analysis identified the presence of esters, ethers and carboxylic acids in Calyx and alkyne, alkane, hydroxyl and amide groups in the seeds. In addition, the mass was conducted using four different empirical models, and the power model was found to be the most suitable. The present research will considerably broaden our understanding and be instrumental in creating post-harvest machinery that will increase its value and enable its commercial production.

Quality Assessment and Comparative Study of Value-added Giblet Pickle Incorporated with Red Calyces of Roselle (Hibiscus subdariffa) and Acetic Acid

Background: The present study was carried out to develop a suitable formulation for value added pickle out of chicken giblet by incorporating roselle and evaluate their physico-chemical properties, sensory attributes and shelf-life qualities through microbial count, extend of proteolysis and fat rancidity after packaging in normal food grade polyethylene packaging material stored at ambient temperature. Methods: The developed value added giblet pickle was standardised with 15% rosella. The comparison was made among the samples incorporated in paste, oven dried powdered and 4% acetic acid. Result: The addition of 15% roselle in paste form increased the overall acceptability as well as the product yield. The protein content of both the treatments was found to be higher compared to the control. The pH of control (3.06±0.04) ranged to 4.25±.07 in treatment. The water activity did not differ significantly in control and treatments and the value ranged from 0.94±0.12 to 0.95±0.04. Thiobarbituric acid value (TBARS), tyrosine values and microbial count significantly (p less than 0.05) increased during storage days in all the pickle samples. However, the increase in TBARS, tyrosine values and microbial load were within the recommended limits as per FSSAI for pickled meat products. From the above study, it can be conclude that value-added giblet pickle with good nutritive value and higher acceptability can be prepared by incorporating roselle at 15% level.