Rooting Medium Research Articles

Influence of CuO nanoparticles on photosystem II structural stability and functional activity of corn (Zea mays L.) under drought stress

Photoinhibition and photooxidation of photosystem II (PSII) is one of the key damages caused by drought stress. The present study aimed to assess whether or not foliar application of copper oxide nanoparticles (CuO NPs) is effective in promoting PSII stability and activity in corn plants under drought stress. Three-week old plants of corn were subjected to drought stress and varying levels of CuO nano-particles (0, 25, 50, and 100 mM) of the rooting medium. In this study, the effects of foliar application of copper oxide nanoparticles were assessed on growth, plant water status, nutrient uptake and structural stability of photosystem II of drought stressed corn plants. Drought stress impeded overall growth of the corn plants by reducing leaf relative water content, water potential, chlorophyll a content, and accumulation of K+ in plant leaves and roots. Exogenous application of 25 mM CuO NPs significantly enhanced the growth of corn. Exogenous application of CuO NPs improved the dry biomass and length of roots of the corn plants under drought stress. Although the application of nano-particles did not change leaf photosynthetic pigments, relative water content, K+ accumulation, it enhanced the accumulation of K+ in roots. Drought stress did not affect the structural stability of PSII, but it reduced its activity (performance index, PIABS) due to changes in the reaction center density and the biochemical reaction efficiency or electron transport capability. Exogenous application of CuO NPs improved PIABS due to increase in active reaction center density and electron transport efficiency. However, 100 mM CuO NPs application caused PSII damage at the donor end and reduced active reaction center density in the corn plants under both normal and drought stress conditions. The present findings provide a baseline information that foliar application of CuO-nanoparticles in low concentrations can improve the growth of corn by improving accumulation of K+ and increasing PSII activity. Further research is required to explore its effect on cellular redox balance and activities of PSII and PSI, and optimum dose of CuO-nanoparticles for developing formulations for their commercial applications in farmers’ fields.

Establishment of a Highly Efficient Micropropagation System of Aquilaria crassna Pierre ex Lecomte

Aquilaria crassna Pierre ex Lecomte is a principal species renowned for its production of agarwood. However, the active components of agarwood are not universally in compliance with the standards set by the Chinese Pharmacopoeia. We have identified an elite A. crassna tree with agarotetrol and alcohol extract levels that exceed these standards and have successfully established a stable in vitro micropropagation system using stem segments from this elite tree. The effects of auxins and minerals on axillary-bud induction, shoot multiplication, and rooting were investigated. The most effective medium for axillary-bud induction was a half-strength Murashige and Skoog (1/2MS) medium supplemented with 0.50 mg/L 6-benzylaminopurine (6-BA), achieving an induction rate of 53.33% with minimal hyperhydricity. The optimal shoot proliferation medium was an MS medium with 0.40 mg/L 6-BA, yielding a propagation coefficient of 2.96 without hyperhydricity. The best rooting medium comprised quarter-strength MS (1/4MS) macroelements and 1/2MS microelements with 0.10 mg/L naphthaleneacetic acid (NAA), resulting in an 82.54% rooting rate. Substrate effects on transplant survival and growth were also evaluated, and peat soil was identified as the best substrate, achieving a survival rate of 96.67%. This study introduces a straightforward and efficient in vitro micropropagation system utilizing mature A. crassna as explants. It holds significant importance for the consistent production of agarwood that complies with the standards of the Chinese Pharmacopoeia and provides a model for the targeted breeding of medicinal plants.

Modulating the Rhizosphere Medium and Indole − 3−butyric Acid Supply Influence Rooting, Nutrients and Biochemical Constituents and Histological Features of Pedilanthus tithymaloids

AbstractPedilanthus tithymaloids is a popular temperate indoor succulent ornamental plant and is highly marketable. However, the appropriate propagation techniques should be adopted to solve the rooting issues of cuttings. Therefore, the present research was designed to specify the optimal indole − 3−butyric acid (IBA) concentration and rooting medium type for obtaining good rooting and healthy plants. Three IBA levels (0, 1.0, 2.0, and 3.0 g L− 1) and five growing mediums (sand, sand: clay, sand: peat moss, sand: perlite, and sand: vermiculite as 1:1, v/v) were used. The experiment was performed in a randomized complete block design in three replications. The obtained data illustrated that roots number per cutting and rooting percentage reached the maximum values by treating P. tithymaloids cuttings with 3.0 g L− 1 IBA x sand: perlite or sand: vermiculite medium. Cultivating the treated-IBA 3.0 g L− 1 cuttings of P. tithymaloids in sand: perlite showed the most enhancements in plant height, branches number and leaves number, leaf area and herb dry weight. However, the heaviest root dry weight was noticed with IBA 3.0 g L− 1 x sand: peat moss medium. Except sand medium, all other tested media in combination with IBA at 1.0, 2.0–3.0 g L− 1 showed remarkable stimulation effect on chlorophyll content. Sand: vermiculite mixture x IBA at 2.0 g L− 1 was the effective practice for improving carbohydrates percentage. By studying the anatomical changes of the adventitious root of P. tithymaloids plant, it was found that there was an increase in the root diameter, phloem and xylem tissues and xylem vessels diameter due to growing in sand: perlite x 3.0 g L− 1 IBA. In conclusion, dipping the cuttings bases of P. tithymaloids in 3.0 g L− 1 IBA and cultivation in sand: perlite considered the most effective combination practice for better rooting ability and growth and could be advisable for commercial production.

In vitro cluster buds regeneration and control of shoot tip necrosis in tissue cultures of Trichosanthes cucumerina L.

Trichosanthes cucumerina L. is a medicinal vine with great potential that can be used as a vegetable. In this study, an efficient in vitro regeneration system of T. cucumerina was established by inducing adventitious buds and controlling Shoot Tip Necrosis (STN) in the in vitro culture. The results of hormone single factor and orthogonal experiments showed that 6-BA had a significant effect on inducing adventitious buds. The best medium for bud proliferation was MS + 6-BA 3.0 mg·L− 1 + KIN 2.0 mg·L− 1 + NAA 0.5 mg·L− 1, and the proliferation capacity was 14.68. However, after three consecutive subcultures, a higher incidence of STN (78.86%) was observed. The results of the STN control experiment showed that reducing the concentration of inorganic salt, shortening the subculture interval, and increasing the Ca2+ in the medium could effectively control the occurrence of STN. However, reducing the concentration of inorganic salt and shortening the subculture interval excessively reduces the proliferation capacity significantly. Considering both the incidence of STN and proliferation capacity, the measures proposed in this study to alleviate STN were to keep the concentration of inorganic salt unchanged, adjust the subculture interval from 45 days to 40 days, and increase the Ca2+ concentration in the medium to 480 mg·L− 1. When the subculture interval was 40 d, the stem segments obtained the lowest STN incidence (8.3%) and higher shoot proliferation capacity (10.02) on the medium of MS + 3.0 mg·L− 1 6-BA + 2.0 mg·L− 1 KIN + 0.5 mg·L− 1 NAA + 360.36 mg·L− 1 Ca2+ (360 mg·L− 1 is the concentration of additional calcium to be added, and after this addition, the total calcium concentration in the MS medium was 480.48 mg·L− 1). The best rooting medium for stem segments was MS + 0.50 mg·L− 1 NAA; the rooting rate was 100%, and no STN occurred. After acclimation, the survival rate of the rooted plantlet reached 95% after 40 days in the soil matrix of coconut husk: humus soil: perlite = 2: 2: 1 (v/v). The results of this study not only reveal the mechanism of STN in T. cucumerina, but also provide technical support and a theoretical basis for its further breeding and cultivation.

Rooting Medium and Indole-3-Butyric Acid (IBA) Stimulate Adventitious Rooting in Codiaeum ‘Aucubaefolia’

The bright coloring of Codiaeum 'Aucubaefolia' leaves has attracted high demand in the floriculture crop industry. The stimulatory effect of rooting hormone (indole-3-butyric acid; IBA) and rooting media on adventitious root growth of 'Aucubaefolia' was evaluated. The study was conducted as two experiments to determine the rooting performance of ‘Aucubaefolia’ stem cuttings. The first experiment was designed to test the rooting performances of unrooted cuttings after a 48-hour cold storage condition at 21°C. The influence of rooting media and IBA on the development of rooted cuttings was assessed in experiment two. Coir dust and jiffy pellet were tested as two media and 0 mg·L-1, 250 mg·L-1, 500 mg·L-1, and 1000 mg·L-1 were tested as four concentrations of IBA. The most significant effects were shown by rooting media on the rooting of ‘Aucubaefolia’ stem cuttings. Among the two media, the jiffy pellet reported the highest number of roots per cutting, root-to-shoot ratio, and average root diameter. The coir dust medium showed the minimum days to root initiation. The application of 500 mg·L-1 IBA experienced the highest number of roots in jiffy pellet medium. Subsequently, the vegetative propagation of C. variegatum ‘Aucubaefolia’ using stem cuttings was significantly influenced by the rooting medium and IBA concentration.

In vitro propagation of triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl

AbstractTriploid lacquer trees have a higher yield of raw lacquer and greater stress resistance; however, it is challenging to develop efficient and rapid seedling culture techniques because of the influence of metabolites with raw lacquer. In this study, the stem segments of a triploid lacquer tree were used to investigate the effects of explant sterilization treatment, different combinations of exogenous hormone concentrations on the induction of axillary buds, seedling development, and rooting, as well as transplanting substrate ratios. The results showed that the optimal combination of disinfection treatment for triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl explants involves 75% alcohol immersion for 10 s, followed by 2.0% benzalkonium chloride treatment for 1 min and 0.1% HgCl2 treatment for 4 min. The suitable medium for axillary bud induction was Murashige and Skoog (MS) containing 0.1 mg•L−1 zeatin (ZT) and 0.1 mg•L−1 6-benzylaminopurine (6-BA), resulting in a high induction rate of 95.0%. Use of 1/2 MS supplemented with 0.5 mg•L−1 6-BA and 0.3 mg•L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was the most effective for axillary bud proliferation. The combination of 1/2 MS containing 0.1 mg•L−1 6-BA, 0.5 mg•L−1 2,4-D, 0.5 mg•L−1 indole-3-butyric acid (IBA), and 0.2% activated carbon (AC) was an optimum for hardening-off. With woody plant medium (WPM) supplemented with 0.5 mg•L−1 naphthaleneacetic acid (NAA) and 0.5 mg•L−1 2,4-D and 0.5 g•L−1 AC as a rooting medium, the rooting rate was 67.8%. Also, the appropriate transplanting substrate ratio for triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl was grass charcoal:laterite:humus = 1:1:1. Collectively, the results provided the technical assurance for seedling scale multiplication and propagation, facilitating its application in production.

Impact of Iba and Naa Concentrations on Growth Characteristics of MR2 Mulberry (Morus sinensis) Using Mini-Clonal Technology at Nursery Level

Using mini-clonal technology, an experiment was carried out to examine the effects of rooting hormone on the growth and rooting characteristics of the mulberry (Morus sinensis L.) variety 'MR2'. Cuttings from the apical shoots of the mulberry variety MR2, which is the popular variety among farmers and have good resistance towards mildew attack was taken for the study. The experiment was conducted at the Department of Sericulture, Forest College and Research Institute, Mettupalayam. Four replications of the Completely Randomized Design (CRD) experiment were used to arrange the data. Two hormones, such as Indole-3-butyric acid (IBA) and Napthalene acetic acid (NAA), were used in the experiment. The hormones were replicated four times in each of the following concentration ranges: 1000, 2000, 3000, 4000, and 5000 ppm. Different hormone concentrations were applied to apical shoot cuttings before they were planted in the appropriate rooting medium in a greenhouse. At the 90th day, measurements were taken of the following parameters like shoot length (cm), number of roots per plant (no’s), root length (cm) and number of leaves per plant (no’s). IBA and NAA at 5000 ppm out performed all other treatments in terms of mulberry mini cutting growth and root characteristics.

Effects of Nitrogen Content and pH on Micropropagation of Rudraksha (Elaeocarpus ganitrus Roxb.)

This study evaluated the effects of pH and inorganic nitrogen sources (KNO3 and NH4NO3) on the regeneration of Elaeocarpus ganitrus Roxb. The pH and nitrogen concentration in the medium were crucial for the successful micropropagation of E. ganitrus. The concentration of nitrogen in the medium significantly affected the development and growth rate of cultures. As a basal medium, Murashige and Skoog's medium (MS) was employed together with various nitrogen concentrations and plant growth hormones. After 4-5 weeks of inoculation, BAP (0.5 mg/l) and NAA (0.1 mg/l) produced the maximum number of shoots (4.42 ± 2.17) among all the growth hormones tested. To obtain the maximum number of shoots and shoot length, different strengths of KNO3 (500-3500 mg/l), NH4NO3 (500-3500 mg/l), and pH (5-7.5) were adjusted in the medium. The largest number of roots was contained in MS medium with 1.0 mg/l NAA (4.47 ± 1.38) after root induction was carried out on MS with NAA, IBA, and IAA (0.2-2.0 mg/l). When the medium contained 1500 mg/l KNO3 and 2000 mg/l NH4NO3 (3.02 ± 1.60), the highest root initiation was observed. The rooting medium with a pH of 6.0 had the most influence and produced the maximum number of roots (4.8 ± 0.48). The plantlets were allowed to harden and acclimate in a greenhouse. A 76% survival rate was noted in the field.

Influence of rooting hormone and rooting substrate on growth of apical shoot cuttings of mulberry (Morus indica l.) Using mini clonal technology at nursery level

An experiment was conducted at Department of Sericulture, Forest College and Research Institute, Mettupalayam to analyse the effect of rooting hormone and their concentrations, rooting medium on apical shoot cuttings of mulberry. Auxin with various concentrations namely IBA and NAA (1000, 2000, 3000, 4000 and 5000 ppm) and rooting mediums such as Soil, Soil: Sand: FYM (1:1:1), Soil: Coir pith (1:1), Soil: Sand: Vermicompost (1:1:1) and Soil: Coir pith: FYM (1:1:1) were used for this study. Mulberry apical cuttings were treated with different hormonal concentrations and placed in different rooting medium under shade house condition. After planting survival per cent, sprouting per cent, rooting per cent and shoot length of the plants were examined. Among the various interaction effect, IBA at 3000 ppm treated plants placed in Soil: Coir pith: FYM medium performed well in all parameters.

Comparative Analyses of Green Plantlet Regeneration in Barley (Hordeum vulgare L.) Anther Culture

The efficient doubled haploid (DH) plant production methods play a key role in accelerating the breeding of new varieties and hybrids in cultivated plants. Consequently, DH plant production methods are continuously improving for barley (Hordeum vulgare L.) breeding and research programs. Two plant regeneration (FHGR and K4NB) and three rooting media (MSr, N6I and ½N6I + Ca) were compared with four F1 barley cross-combinations to clarify the effect of medium on the regeneration of green and albino plantlets and acclimatization. The plant regeneration efficiency was higher using K4NB medium (74.53 green plantlets/100 anthers and 30.85 albino/100 anthers) compared to FHGR (55.77 green plantlets/100anthers and 21.32 albino/100 anthers). The percentage of acclimatization was highest when the K4NB regeneration medium was combined with the MSr rooting medium. Altogether, 61.83% of the anther culture-derived plantlets of 8 cross-combinations acclimatized to the greenhouse conditions, and 1403 acclimatized plantlets were produced from the F1 cross-combinations. Haploid (22.52%), diploid (69.37%) and tetraploid (8.11%) plantlets were identified among the 111 tested green plantlets by flow cytometric analyses. The tetraploid lines can be explored to offer new scopes for future barley research and breeding directions. Nearly one thousand DH plants have been integrated into our barley breeding program.

Effect of landraces on Musa micropropagation

This work aimed at assessing the performance of two landraces of Musa spp in micropropagation. Tissues from Owom and Efol were sterilized by soaking the explants in 70 % ethanol for 30 sec, 8 % NaOCl for 5 min, 5 g/L benlate for 5 min, and in 1.2 g/L HgCl2 for 10 min before being thoroughly washed with distilled water. Tissue exposure to U. V. light for 5 min was done before they were cultured in a shooting MS-based culture medium supplemented with 5.00 mg/L concentration of 6-Benzylaminopurine, and later to a rooting MS-based culture medium supplemented with indoleacetic acid (IAA) of 2.00 mg/l concentration. Data were collected on number of shoots, shoot height, shoot’s health, days to rooting, root number, and root length. The result from shooting medium showed that the plants’ heights were highly significantly different at P ≤ 0.01, with Owom producing marginal mean heights of 3.59 cm and 5.05 cm at 3 and 5 weeks respectively, after initiation. A very high significant difference was observed with plant health at P ≤ 0.001 whereby Owom landrace reduced in health status as the week increased, unlike Efol which increased in health status across the weeks. For rooting medium, significant differences at P ≤ 0.05 for days to rooting, root length, health performance, and plant height were observed. While high significant difference was observed for the number of roots at P ≤ 0.01. Owom performed significantly better than Efol and has shown value for commercial production and breeding programs.

Determining the Effect of Rooting Media and Different Polythene Wrappers on Air Layering of Grape Fruit (Citrus paradisi)

The present study aims to investigate the “EFFECT OF ROOTING MEDIA AND DIFFERENT POLYTHENE WRAPPERS ON AIR LAYERING OF GRAPE FRUIT (Citrus paradisi’)”at The Department of Horticulture, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, during the period 2023-24. The experiment was laid in completely randomized block design with three replications and ten treatment combinations. viz, T1 (BA1000PPM + BLACK POLYTHENE + SPHAGNUM MOSS) ,T2 (IBA1000PPM + BLACK POLYTHENE + SAWDUST), T3 (IBA1000PPM + BLACK POLYTHENE + GARDEN SOIL+ SPHAGNUM MOSS),T4 (IBA1000PPM + BLACK POLYTHENE + SPHAGNUM MOSS+ SAWDUST), T5(IBA1000PPM + BLACK POLYTHENE + GARDEN SOIL+ SAWDUST), T6 (IBA1000PPM + WHITE POLYTHENE + SPHAGNUM MOSS), T7 (IBA1000PPM + WHITE POLYTHENE + SAWDUST),T8(IBA1000PPM + WHITE POLYTHENE + GARDEN SOIL + SPHAGNUMMOSS), T9(IBA1000PPM + WHITE POLYTHENE + GARDEN SOIL + SAWDUST),T10(IBA1000PPM + WHITE POLYTHENE + SPHAGNUM MOSS + SAWDUST). On the basis of present experimental findings, it is concluded that treatmentT4(IBA1000PPM + BLACK POLYTHENE + SPHAGNUM MOSS+ SAWDUST) was found to be best in terms of Number of days to root formation (initial rooting), Root length(cm), Root thickness(mm), Number of roots per layer, Survival percentage, Number of new sprouts per layered plant, Number of new leaves per layered plant and length of new shoot(cm) in Grape fruit.Whereas T9(IBA1000PPM + WHITE POLYTHENE + GARDEN SOIL + SAWDUST) was found to be least in terms of Number of days to root formation (initial rooting), Root length(cm), Survival percentage, Number of new sprouts per layered plant, Number of new leaves per layered plant and Length of new shoots(cm) whereas in Root thickness(mm) and number of roots per layer in Grape fruit.

Efficient In Vitro Regeneration System and Comparative Transcriptome Analysis Offer Insight into the Early Development Characteristics of Explants from Cotyledon with Partial Petiole in Small-Fruited Pepper (Capsicum annuum).

In our research, we utilized six small-fruited pepper germplasms as materials, selected cotyledons with the petiole and hypocotyls as explants, and conducted in vitro regeneration studies. Our outcomes specify that the most suitable explant is cotyledon with the petiole, and the suitable genotype is HNUCA341. The optimal medium for inducing and elongating adventitious buds for this genotype is Murashige and Skoog medium (MS) + 9.12 μM Zeatin (ZT) + 0.57 μM 3-Indoleacetic acid (IAA), with a bud induction rate of 44.4%. The best rooting induction medium is MS + 1.14 μM IAA, with a rooting rate of 86.7%. Research on the addition of exogenous hormones has revealed that the induction speed of buds in small-fruited pepper (HNUCA341) in the combination of ZT and IAA hormones (abbreviated as ZI) is quicker, and the induction effect is better. The histological observations indicate that ZI treatment accelerates the initiation of explant division and differentiation, causing a shorter duration of vascular-bundle tissue production. The plant hormone signaling pathway was significantly enriched by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, including ARR9 (LOC107843874, LOC107843885), ARR4 (LOC107848380, LOC107862455), AHK4 (LOC107870540), AHP1 (LOC107839518), LAX2 (LOC107846008), SAUR36 (LOC107852624), IAA8 (LOC107841020), IAA16 (LOC107839415), PYL4 (LOC107843441), and PYL6 (LOC107871127); these significantly enriched genes may be associated with in vitro regeneration. In addition, the carbon metabolism pathway and plant mitogen-activated protein kinase (MAPK) signaling pathway are also significantly enriched in KEGG. The results of the Gene Ontology (GO) analysis revealed that differentially expressed genes related to carbon metabolism and fixation, photosynthesis and MAPK signaling pathways were upregulated under ZI treatment. It was found that they might be associated with enhanced regeneration in vitro. Furthermore, we also screened out differentially expressed transcription factors, primarily from the MYB, bHLH, AP2/ERF, and NAC families. Overall, our work accumulated important data for the in-depth analysis of the molecular mechanism of in vitro regeneration of pepper, and provides valuable germplasm for establishing an efficient stable pepper genetic-transformation system based on tissue culture.

The Effect of Grass Juices on The Agricultural Properties of Legumes Grown in Different Root Media

The consumption of grass juices obtained from cereals for health purposes is increasing day by day. For this purpose, research on different plant solutions has gained momentum. In our study, barley (Hordeum vulgare L.) and oat (Avena sativa L.) grass juices grown in hydroponic conditions were used. Obtained grass juices were applied to pea and lupine seeds in 100% soil (control), 100% pumice, 100% perlite, 100% tea waste and 100% cocopeat substrate. Germination percentage, germination rate, average daily germination, peak value, germination value, root dry and fresh weight, stem dry and fresh weight, root and stem length, plant yield, grass yield, plant yield seed rate, macro and micro (N, P, K, Ca, Mg, Fe, Cu, Zn and Mn) mineral substance contents were investigated pea and lupine. Grass juices were obtained by mowing barley and oat seeds sown in 30 × 50 × 7 cm plastic tubs in ten days and passing them through a juicer. Pea and lupine 25 seeds, which were placed in each pot, were grown in different root media (100% soil, 100% cocopeat, 100% pumice, 100% perlite, and 100% tea waste) under in vitro conditions. 150 ml of water (control), barley grass juice, oat grass juice and barley + oat grass juice were applied to the seeds according to their subjects. The experiment was carried out in 400 pots with 10 replications x 2 species x 4 treatments x 5 media, according to the factorial fully randomised design. As a result of the research, germination physiology (germination percentage, germination rate, average daily germination, peak value and germination value), mineral substance content (N, P, K, Ca, Mg, Fe, Cu, Zn and Mn), growth and yield parameters at (root dry and wet weight, stem dry and wet weight, root and stem length, plant yield, grass yield, plant yield seed rate) barley grass juice application was found to be more effective than other applications after control. In all parameters examined in the research, it was determined that the five different medias used as growing media followed the order of soil>cocopeat>pumice>perlite>tea waste, and after the control and the best growing medium was cocopeat.

Effect of auxin and media for rooting of different fruit crop cuttings: A review

Effect of auxin and media for rooting of different fruit crop cuttings: A review

A Rapid and Cost Effective Hardening Method for Tissue Culture Derived Banana Plantlets

The present study was aimed to develop a cost effective method for the hardening of tissue culture derived plants of banana var. Robusta. Two approaches were investigated to reduce cost. First, the shoots inoculated in rooting medium were incubated in (i) growth room under controlled temperature and light conditions for four weeks (RL) (ii) shadehouse exposed to natural light and ambient temperature (RP). The plants obtained from these treatments were given five hardening treatments (a) direct planting in polybags (filled with 1 : 1 Soil and leaf mould) (DP), (b) DP plants covered individually with transparent polybags (DPC), (c) DPC plants placed in tray of water (up to 3 cm height)-DPCW (d) DP plants kept in polytunnel (DPP) (e) DP plants placed in tray of water and kept in polytunnel DPWP, (f) control plants (CN)-primary hardened in potrays kept in polytunnel for four weeks followed by transplantation to polybags (filled with3:1 Soil and leaf mould). RP Plants were greener and sturdier with less hyperhydricity. Rooting outside the lab (RP) reduced the cost as the growth room space during rooting could be saved and so there is saving in electricity of lighting and climate control. 80% of plants in control and 50% plants in direct planting (DP) survived. In all other treatments 100% survival was observed. RP-DPCW and RP-DPWP plants showed the best performance in terms of visible appearance and accumulation of biomass. The method employed could reduce the hardening period from 12 weeks (CN) to 8 weeks. The cost reduction occurs in this process by a) omitting primary hardening process which requires costly potting media, manpower and climate controlled greenhouse, b) Lab space could be saved as incubation for rooting can be done in shade house thereby saving electricity c) As there is 4 weeks saving in hardening, shade house space could be utilized for hardening more plants. The method could be adopted for hardening in small nurseries to large industries and can result in cost reduction of plantlets. Saving of 4 weeks for hardening will be highly beneficial to the micropropagation industries for delivering plants to farmers in time during peak planting seasons. Plant Tissue Cult. & Biotech. 34(1): 11-24, 2024 (June)

Effects of Different Rooting Medium and Growth Regulating Agents on Rooting Parameters of Cuttings of Lavender and Lavandin Cultıvars (Lavandula sp.)

This study was conducted in Çanakkale province (North-West Anatolia) to find the optimal auxin dosages (IBA 2000 ppm and NAA 1000 ppm) and rooting medium (soil, peat, and cocopeat) for cutting propagation of lavender, which has great industrial and commercial value. Hemus, Sevtopolis and Drujba varieties belonging to the Lavandula angustifolia species and Super A variety belonging to the Lavandula intermedium species were used as study materials. The experiment was set up with 3 replications according to the split-plot trial design divided into random plots. As a result, the Super A variety of Lavandula intermedium species cultivated in cocopeat medium following IBA 2000 ppm administration had the highest rooting rate (88.33%) and rooting status (17.67 units). The Sevtopolis variety of the Lavandula angustifolia species produced the highest root number (15.33 pieces), root length (9.88 cm), shoot number (20.93 pieces), and shoot length (15.35 cm) values when grown in cocopeat medium with IBA 2000 ppm application. According to these results, cocopeat medium and IBA 2000 ppm auxin application came to the fore in the propagation of lavender by cuttings.

Bioactivity assessment of high potency arachidin mixture from large-scale peanut hairy root culture medium

Bioactivity assessment of high potency arachidin mixture from large-scale peanut hairy root culture medium

In vitro mass propagation of Dendrocalamus asper (Giant bamboo) through direct organogenesis

Dendrocalamus asper (giant bamboo) is a clumping type of bamboo belonging to the Poaceae family. Due to its economic and environmental value, demand for this species has increased tremendously. Conventional propagation methods have limitations due to low seed viability and the lack of healthy clumps. Therefore, an in vitro mass propagation protocol was developed to provide healthy plants for large-scale plantations. Seeds were used as the explant and they were surface sterilized and cultured on MS medium free of growth regulators. Nodal segments taken from in vitro germinated seedlings were used for shoot initiation. The best medium for shoot induction (MS medium supplemented with 0.0–2.5 mg/L BAP), best medium for multiple shoot induction (MS medium supplemented with 0.0–5.0 mg/L BAP), effect of shoot cluster size (shoot clusters containing 1–4 shoots) and effect of physical state of the medium (semisolid and liquid media) on multiple shoot induction were determined using shoots per node, mean shoot length and mean number of leaves per shoot after 6 weeks of incubation. Elongated shoots were transferred into a rooting medium and the best medium for root induction (MS medium supplemented with 0.0–5.0 mg/L IBA and IAA) and effect of cluster size (shoot clusters containing 1–4 shoots) on rooting were determined using the number of roots and root length after 6 weeks of incubation. All the cultures were maintained under a 16-hour photoperiod. Well-developed plantlets were transferred to coir pellets and after four weeks transferred into different potting mixtures containing different combinations of sand, compost and coir dust. Unless otherwise mentioned there were at least twenty replicates in all treatments. The highest mean number of shoots per node (16.87±0.52), mean shoot length (4.12±0.27 cm) and mean number of leaves per shoot (4.80±0.33) were observed in the MS medium supplemented with 1.0 mg/L BAP. The MS medium supplemented with 2.0 mg/L BAP was the best for multiple shoot induction, shoot cluster with 3 shoots was the best cluster size and the liquid medium had a better effect on shoot multiplication. The half-strength MS medium supplemented with 2.0 mg/L IBA was the best for in vitro root induction with the highest mean number of roots (7.15±0.77) and a mean root length of 10.79±1.11 cm. Shoot clusters with 3 shoots was the best cluster size for root induction. A sand: compost: coir dust (1:1:1) mixture was the best potting mixture giving 100% survival. These findings provide a reliable micropropagation protocol for D. asper, which holds great promise for meeting the growing demand for bamboo resources and promoting sustainable bamboo cultivation.

Effect of auxins on biochemical composition of apical cuttings of Mulberry (Morus indica l.) Using mini clonal technology

A research study was carried out to examine the effects of auxin and rooting hormone on the biochemical composition of the mini clonal mulberry leaves raised using mini-clonal technique of (Morus indica L.) variety 'V1'.Two hormones, such as indole-3-butyric acid (IBA) and napthalene acetic acid (NAA), were used in the experiment. The hormones were replicated four times in each of the following concentration ranges: 1000, 2000, 3000, 4000, and 5000 ppm. Different hormone concentrations were applied to apical shoot cuttings before they were planted in the appropriate rooting medium in a greenhouse condition. The biochemical parameters analysed during the research study include leaf moisturecontent, nitrogen, phosphorous, potassium, chlorophyll-a, chlorophyll-b, carotenoid, total chlorophyll, crude protein, total carbohydrates, crude fat, total protein content present in mulberry leaves. The nutritive value of mulberry varieties depends on its biochemical constituents present in it. The results indicated that V1 mulberry mini clones have recorded better biochemical constituents and suitable for rearing of mulberry silkworms to get successful cocoon crop.