Root Growth Capacity Research Articles

Optimizing Initial Nitrogen Application Rates to Improve Peanut (Arachis hypogaea L.) Biological Nitrogen Fixation

The application of nitrogen fertilizer is crucial to the growth and biological nitrogen fixation of peanut, especially in the seedling stage where nodules have not yet formed. However, it is still uncertain how much initial nitrogen fertilizer should be applied to promote peanut root growth, nodule formation, and biological nitrogen fixation (BNF). There, a 2-year pot experiment was conducted using Huayu 22 (HY22, large-grain cultivar) and Huayu 39 (HY39, small-grain cultivar) as experimental materials to research the effects of different initial nitrogen fertilizer application rates on peanut root growth (root weight, root length, root mean diameter, root activity) and biological nitrogen fixation capacity (nodule number, nodule weight, biological nitrogen fixation, and nitrogen fixation potential per plant). N0, as control, four initial nitrogen fertilizer application rates were established: 15 kg·hm−2 (N15), 30 kg·hm−2 (N30), 45 kg·hm−2 (N45), and 60 kg·hm−2 (N60). The present results showed that the nodule number, nodule dry weight, nitrogenase activity, and biological nitrogen fixation of the HY22 cultivar under the N15 treatment were higher compared to those under other treatments over the two growing seasons. In addition, the cultivar of HY39 treated with the N15 treatment also increased the nitrogen fixation potential per plant and BNF relative to other treatments. Although the application of 60 kg·hm−2 nitrogen increased the root surface area and root volume, it decreased the nitrogenase activity, nodule dry weight, and nitrogen fixation potential per plant of HY22 and HY39 varieties in both growing seasons. Above all, an initial nitrogen application of 15 kg·hm−2 may be the optimal treatment for promoting peanut nodule formation and biological nitrogen fixation.

Silicon improves root system and canopy physiology in wheat under drought stress

AimsRoot system is an important regulator for unevenly distributed below-ground resource acquisition. In a rainfed cropping environment, drought stress (DS) significantly restricts root growth and moisture uptake capacity. The fact that silicon (Si) alleviates DS in wheat is widely reported, but its effects on the wheat root system remain unclear.MethodsThe present study investigated the effect of pre-sowing Si treatment on two contrasting wheat cultivars (RAC875, drought-tolerant; Kukri, drought-susceptible) at early growth stages. The cultivars were grown in a glasshouse in a complete randomized design with four replications and two watering treatments. Various root traits and physiological data, including non-destructive infrared thermal imaging for water stress indices, were recorded.ResultsUnder DS and Si (DSSi), Kukri had a significant increase in primary root length (PRL,44%) and lateral root length (LRL,28.1%) compared with RAC875 having a substantial increase in PRL (35.2%), but non-significant in LRL. The Si-induced improvement in the root system positively impacted canopy physiology and significantly enhanced photosynthesis, stomatal conductance and transpiration in Kukri and RAC875 under DSSi. Canopy temperature was reduced significantly in Kukri (4.24%) and RAC875 (6.15%) under DSSi, while canopy temperature depression was enhanced significantly in both the cultivars (Kukri,78.6%; RAC875, 58.6%) under DSSi.ConclusionThese results showed that Si has the potential to influence below-ground traits, which regulate the moisture uptake ability of roots for cooler canopy and improved photosynthesis under DS. It also suggests a future direction to investigate the underlying mechanisms involved in wheat’s Si-induced root growth and moisture uptake ability.

Assessing the potential toxicity of hazardous material released from Pb-based perovskite solar cells to crop plants

Perovskite solar cells are of interest to the solar power industry because of their comparatively low cost and simple manufacturing process. Large-scale solar arrays are typically located near arable land. However, limited data on the toxicity of perovskite compounds are currently available. This study investigated the effect of potentially hazardous chemicals from Pb-based perovskite solar cells on two crop plants (mungbean and sorghum). The direct exposure of a single chemical (PbI2) in soil media was simulated, a plant soil assay was conducted, and phytotoxicity was subsequently evaluated. After 21 days of exposure, toxicity symptoms in plant growth and physiological responses were observed. The results show that plant root and shoot growth and photosynthetic capacity all remarkably decreased by chemical exposure. Both Pb and I were found to be associated with toxicity symptoms. The reactions of these crop plants to PbI2 exposure indicate the potential phytotoxicity of leachable compounds from Pb-based perovskite solar cells; further, the results obtained in this study will contribute to the assessment of risks associated with the use of these cells in large-scale commercial solar arrays.

Elevated CO2 Influences the Growth, Root Morphology, and Leaf Photosynthesis of Cacao (Theobroma cacao L.) Seedlings

Growing quality seedlings is a challenge for sustainable cacao production as the survival rate of young seedlings is strongly influenced by environmental factors that affect the productivity of cacao farmers. In this study, cacao (Theobroma cacao L.) seedlings were cultivated in a nursery, and the effects of elevated CO2 concentrations (approximately 800 ppm) applied to cacao seedlings during daytime (6:00–17:59) on the root growth, morphology, and leaf photosynthetic capacity were examined. Treatment with elevated CO2 significantly improved root growth, dry matter weight, and root/shoot ratio. Three-dimensional imaging of roots showed that lateral roots grew longer horizontally, lateral roots and fine roots were distributed over a larger area, and root surface and root volume increased significantly under elevated CO2 treatment. Accurate quantification of root morphology using X-ray CT indicated that the treatment with elevated CO2 concentrations may significantly affect root quality during the seedling stage by expanding the distribution range of lateral and fine roots, which increases the ability of lateral roots to elongate and absorb water and nutrients from the superficial layers. The photosynthetic characteristics of the aboveground leaves of cacao seedlings exposed to elevated CO2 concentrations showed a tendency to adapt to elevated CO2 concentrations by increasing light-use efficiency and CO2-use efficiency. Therefore, the treatment of cacao seedlings with elevated CO2 concentrations improved the growth quality of seedlings due to the characteristics of the roots as large sinks.

Root frost tolerance, seasonal variation in root growth, and field performance of one-year-old Russian larch seedlings with simulated root freezing damages

ABSTRACT Planting healthy seedlings with optimal growth potential is essential for proper growth and survival in forest regeneration. Assessing the seedling quality prior to planting is therefore important. In this Icelandic study, effects of root damage induced with artificial freezing in young Russian larch seedlings were examined using the root growth capacity method (RGC). Frost tolerance of roots varied during the winter, and root growth in undamaged seedlings fluctuated, indicating seasonal variations in growth rhythm. The LT50 value for root frost tolerance was −13.9°C in late January, but already at −10.6°C (LT10) root damages were severe. After one growing season, shoot elongation was significantly lower in seedlings frozen to −9°C, −13.5°C, and −15.5°C by 23%, 54%, and 72%, respectively, compared with undamaged seedlings. Control seedlings and seedlings frozen to −9°C achieved 100% survival after the first growing season. Survival in seedlings frozen to −13.5°C and −15.5°C was 85% and 27%, respectively. After the second growing season, survival decreased in all frost-damaged seedlings. The ongoing mortality demonstrates the long-lasting effects of planting seedlings with damaged root systems, and the fluctuation in root frost tolerance of young Russian larch seedlings during winter emphasises the need for care when seedlings are moved to outdoor storage.

Evaluation of Recycled Materials as Hydroponic Growing Media

Conventional soilless growing media, such as perlite, are mined from nonrenewable resources and can only be disposed of in landfills after limited use. There is a need to investigate novel, sustainable growing media adapted from waste or engineered to be reused over multiple cycles. This study investigated waste almond shells and a recycled plastic drainage plank as hydroponic growing media alternatives. Physiochemical properties were evaluated, and a germination and greenhouse growth trial was conducted to understand the effect these media have on production and nutritional quality of lettuce (Lactuca sativa L. cv. Catalogna Verde). Drought testing was carried out to understand how the media affected the lettuce’s response to water stress. In comparison to perlite, yields under regular irrigation were reduced by 52% in almond shells and 72% in plastic planks, although lettuce grown in almond shells still obtained commercially relevant yields. Reduced yields in almond shells were likely caused by the shell’s high salinity. Lettuce growth in plastic planks was limited by impeded root growth and low water-holding capacity. In conclusion, with minor alterations, almond shells could be used as a sustainable growing media alternative to perlite in hydroponic lettuce production. More research is needed to manufacture the planks to be conducive to plant growth.

New Plant Growth Stimulants Based on Water-Soluble Nanoparticles of N-Substituted Monoamino-Acid Derivatives of Fullerene C60 and the Study of their Mechanisms of Action

Growth-stimulating effects of water-soluble nanoparticles of N-substituted monoamino-acid derivatives of fullerene C60 (L- and D-alanine, L- and D-valine, L- and D-aspartic acid, β-alanine, and γ-aminobutyric and e-aminocaproic acids in potassium salt form) were investigated. It was found that the nanoparticle size and relative antiradical activity of fullerene derivatives were factors that influence such physiological parameters of field peas as seed germination rate, germination energy, and root growth capacity. It was shown that the relative antiradical activity of nanoparticles in the selected group of compounds was determined by the total surface area of the nanoparticles regardless of the structure of the amino-acid substituent. The possibility of using amino-acid derivatives of fullerene as effective growth stimulating substances has been demonstrated. A dose-dependent effect of N-(monohydrofullerenyl)-D-alanine potassium salt in a concentration range of 10–9–10–11 M on the seed-germination rate and germination energy of field peas has been demonstrated.

An Assessment of Storability of Norway Spruce Container Seedlings in Freezer Storage as Affected by Short-Day Treatment

Determination of safe times at which to transfer seedlings to freezer storage is problematic in forest tree nurseries. The present study aimed to determine the relationship between pre-storage frost hardiness (FH) of different plant parts, dry matter content (DMC), chilling hours (the sum of hours when temperature was between −5 °C and +5 °C), and post-storage vitality, and the impact of short-day (SD) treatment on these relationships. One and a half year old control seedlings and SD-treated seedlings of Norway spruce were transferred to freezer storage (−3 °C) on five occasions during autumn. On each occasion, the FH of buds, needles, stem, and roots, as well as DMC, were determined, and chilling hours were calculated. The vitality of the freezer-stored seedlings was determined through their root growth capacity in the subsequent spring, and through the field performance of the seedlings (shoot growth and seedling damage) at the end of the following two growing seasons. Seedlings were considered to be storable when the FH of the needles was at least −25 °C, and the FH of the roots was about −10 °C in both treatments. Early storage reduced the vitality of the seedlings. SD treatment did not advance the storability of the seedlings, although it alleviated some of the negative effects of early storage by improving the FH of needles and stem, but not that of the roots. The DMC value, indicating storability, was higher for SD-treated seedlings than for control seedlings. When data from five experiments conducted in Suonenjoki were combined, it was found that the relationship between accumulation of chilling hours and needle FH was dependent on nursery treatment and assessment year, which reduces the reliability of using chilling hours in predicting the storability of Norway spruce seedlings. The predicted climate change may complicate the fall acclimation of seedlings. New, user-friendly methods for determining storability of seedlings are urgently needed.

Treatments for induction of cold hardiness in Picea abies (L.) Karst. and Pinus sylvestris L. seedlings pre-cultivated under light-emitting diodes – impact of photoperiod and temperature including energy consumption and seedling quality after cold storage

ABSTRACTIn Nordic climates, the weather allows a narrow time window during spring and summer for forest seedlings to be transplanted from indoor growth to outdoor conditions. If a new method for year-round cultivation under LED lamps is to be successfully introduced in forest nurseries, a cold storage phase for batches produced outside of the vegetation period needs to be included in the concept. Different short-day treatments for induction of cold hardiness in very young seedlings of Picea abies and Pinus sylvestris cultivated solely under LED lamps were investigated. The study compared a combination of two photoperiods (5 h or 8 h) at three different temperatures (5°C, 10°C or 20°C) applied during five weeks. Chlorophyll fluorescence, shoot electrolyte leakage and molecular testing for gene expression of cold acclimation were used to assess the treatments. After a period of three months in cold storage at 2°C, the vitality of the seedlings was evaluated using a root growth capacity test. Lower temperatures during the treatments, especially for Pinus sylvestris, had a significant effect on inducing cold hardiness. The results showed that the photoperiod should not be overly reduced to allow photosynthesis and generation of carbohydrate reserves but it could be optimized to reduce electricity consumption.

Effects of Composite LED Light on Root Growth and Antioxidant Capacity of Cunninghamia lanceolata Tissue Culture Seedlings

We used the 12th generation of the Cunninghamia (C.) lanceolata tissue culture seedlings, and white light emitting diode (LED) light as control (CK). We applied five composite LED light treatments, red-blue 4:1, 8:1 (4R1B and 8R1B), red-blue-purple 8:1:1 (8R1B1P), and red-blue-purple-green 6:1:1:1, 8:1:1:1 (6R1B1P1G and 8R1B1P1G), to study the effects of light quality on root growth characteristics and antioxidant capacity of C. lanceolata tissue culture seedlings. The results showed that: (1) rooting rate, average root number, root length, root surface area, and root activity were higher with 6R1B1P1G and 8R1B1P1G treatments compared to 4R1B, 8R1B, 8R1B1P and CK treatments; and the root growth parameters under the 8R1B1P1G treatment were as high as 95.50% for rooting rate, 4.63 per plant of the average number of root, 5.95 cm root length, 1.92 cm2 surface area, and 145.56 ng/(g·h) root activity, respectively. (2) The composite lights of 4R1B, 8R1B, 8R1B1P, 6R1B1P1G, and 8R1B1P1G are beneficial for the accumulation of soluble sugar content (SSC) and soluble protein content (SPC), but not conducive for the increase of free proline content (FPC); the plants under 6R1B1P1G and 8R1B1P1G treatments had higher superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) activity and lower malondialdehyde (MDA), polyphenol oxidase (PPO) activity. (3) Redundancy analysis showed that POD activity positively correlated with root activity; SPC, SOD and CAT activities positively correlated with root growth parameters; while SSC, MDA content, APX and PPO activities negatively correlated with root growth parameters. These results suggest that the responses of root growth and antioxidant capacity of the C. lanceolata tissue culture seedlings to different light qualities vary. The relationship between root growth parameters and antioxidant capacity was closely related. Red-blue-purple-green was the most suitable composite light quality for root growth of C. lanceolata tissue culture seedlings, and 8:1:1:1 was the optimal ratio, under which the rooting rate, root activity and root growth of tissue culture seedlings peaked.

Testing the potential of LEDs to enhance growth and quality characteristics of Salvia fruticosa

The effect of light-emitting diodes (LED) with broad radiation spectra on developmental, physiological, and phytochemical characteristics of Greek sage (Salvia fruticosa L.) seedlings was assessed. Fluorescent (FL – control) tubes and four LED lights [AP67 (moderate blue, red and far-red), L20AP67 (moderate blue, red and far-red, high green), AP673L (moderate blue, high red) and NS1 (high blue and green, low red, high red : far-red, 1% ultraviolet)] were used in a growth chamber. Seedlings grown under FL, L20AP67 and AP673L exhibited the best morphological and developmental characteristics. FL led to inferior root biomass formation compared to all LEDs. AP67 promoted greater root-to-shoot dry weight ratio and dry-to-fresh overground and root weight ratios, but induced the least morphological and developmental characteristics. NS1 performed well regarding the root biomass production. Total phenolic content and the root growth capacity were not significantly affected. The present study demonstrates that L20AP67 and AP673L LEDs performed equally to FL light regarding the developmental characteristics. AP67 and NS1 may have the potential to be used for compact seedling production.

Evaluating methods for storability assessment and determination of vitality status of container grown Norway spruce transplants after frozen storage

ABSTRACTAutumn sown small seedlings for later transplanting into large containers have been introduced in Swedish forest tree nurseries. Containerized transplants of Norway spruce (Picea abies (L.) Karst.) from three Swedish nurseries were frozen stored during the autumn of 2014 to find out storability and post-storage vitality. Seedling storability was determined by measuring electrolyte leakage after freezing shoots to −25°C (SELdiff−25), by measurements of dry matter content (DMC) of seedling shoots and by the commercial molecular test ColdNSure™. Vitality of seedlings after storage was determined by measuring the leakage of electrolytes from shoots (SEL), and seedlings were also tested in regrowth tests. All three methods for storability assessment gave similar predictions, except in one case where DMC showed “not storable” for successfully stored seedlings. Our results indicated that young transplants can be successfully short term stored before reaching the target levels for safe long-term storage of conventional seedlings. Early storage of young transplants resulted in low post-storage survival and vitality expressed as root growth capacity and shoot electrolyte leakage (SEL). A prolonged duration in storage generally resulted in lower survival as well as lower root growth capacity and higher levels of SEL, especially for seedlings stored at earlier dates.

Light emitting diodes (LEDs) affect morphological, physiological and phytochemical characteristics of pomegranate seedlings

Abstract The impact of light-emitting diodes (LED) on developmental, physiological, and phytochemical characteristics of pomegranate (Punica granatum L.) seedlings was investigated. Five LED lights [L20AP67 (moderate blue and red, high green), AP673L (moderate blue, high red and red:far-red), G2 (low blue, high red and far-red), AP67 (moderate blue, high red), and NS1 (high blue and green, low red, high red:far-red, 1% UV) with wide and continuous radiation spectra were used. Fluorescent [FL (high blue and green, low red)] tubes constituted the control treatment. Among treatments, seedlings grown under L20AP67 exhibited the best morphological and growth characteristics (rapid height increase, longer roots, greater fresh and dry weight, greater leaf area). Root activity was comparable among the various treatments, with FL exhibiting the lowest arithmetic value. A variant effect on pigments and secondary metabolites was recorded with the highest chlorophyll and carotenoid content detected under FL, flavonoid under G2 and AP67, and anthocyanin under G2, AP67 and NS1. In addition, NS1 produced the highest total phenolic compound content. Root growth capacity (RGC) was monitored in specialized chambers, then assessed to evaluate the transplanting capacity. Transplanted seedlings of AP67 had the longest, of L20AP67 the heaviest newly formed roots, thus securing a successful transplantation. The herein undertaken study demonstrates that certain LEDs promoted developmental characteristics in pomegranate more efficiently than conventional FL. In particular, the use of L20AP67 was proven a promising tool for producing robust pomegranate seedlings, while some of the other LEDs tested apparently imposed a stressful situation on plants.

Growth responses of soybean (Glycine max L.) seedlings as affected by monochromic or mixture radiation provided by light-emitting diode

The light emitting diodes (LEDs), as a source of monochromic light, has been widely used in the field of agriculture and horticulture. We conducted a pot experiment to investigate the effect of LEDs (W, B, R1:B1:W1, R3:B1, R5:B1, R6:B1 and R) on growth and photosynthesis of soybean (Glycine max L. viz. Huachun 6 (HC6), Huaxia 3 (HX3) and Guixiadou 2 (GXD2)) seedlings. The agronomic traits, physiological characteristics and photosynthetic capacity of soybean seedlings were determined. The result showed that root growth, photosynthetic pigment, plant height, stem diameter, hypocotyl length, epicotyl length, root dry weight and shoot dry weight were significantly affected with light treatment. Pn was much higher and the root growth was much better under red/blue mixed light (R5:B1) than the other treatment. Photosynthetic pigment of each cultivar was different under various treatments. This study demonstrates that the root growth and photosynthetic capacity of soybean seedling could be regulated by light treatments. Therefore, it is important the effectiveness of light quality for growth and morphogenesis of soybean seedling.

Morpho-functional traits and plant response to drought conditions in seedlings of six native species of Ecuadorian Ecosystems

Tropical Andean ecosystems have been identified as very vulnerable to climate change. Changes in climatic conditions, especially drought as envisaged by climate change projections, could affect the establishment of new individuals that are responsible for ensuring the persistence of species and plant communities. The aim was to study the main morpho-functional traits related to water use and drought resistance of native plant species from Ecuadorian ecosystems during imposed drought periods. Seedlings of six native species representative of the Ecuadorian Andes were studied: lower montane evergreen forest (Alnus acuminata and Cedrela montana), montane cloud forest (Podocarpus sprucei and Aegiphila ferruginea) and montane dry shrubland (Schinus molle and Caesalpinia spinosa). Morphological characterisation, a root growth capacity test and stomatal conductance measurements during two controlled drought periods were used to investigate biomass allocation patterns, the root system morphology and gas exchange patterns of species, respectively. The main results indicated that species-specific differences in morpho-functional traits and allocation patterns determined responses to water availability and drought conditions. Low relative growth rate, leaf area ratio and specific leaf area, and high specific root length, were related to drought-resistant species as P. sprucei and C. spinosa. In contrast, a high or moderate relative growth rate, leaf area and root biomass, but low specific root length as in A. ferruginea, S. molle, C. montana, were related to lower resistance to drought conditions. Despite A. acuminata showed high specific root length, it was reported as a species sensitive to drought. Overall, patterns of stomatal conductance, linked to biomass allocation patterns and root system morphology, especially specific root length, seemed to be related to differences in vulnerability to drought in these species. Drought response was independent of species habitats. Unexpectedly, P. sprucei, belonging to montane cloud forest showed higher drought resistance than S. molle, belonging to montane dry shrubland.

INFLUENCE OF POTASSIUM LEVELS ON ROOT GROWTH AND NUTRIENT UPTAKE OF UPLAND RICE CULTIVARS

ABSTRACT Potassium (K) is an essential nutrient for upland rice growth, but little information on the effects of K on root growth and nutrient uptake capacity of upland rice is found. Therefore, an experiment was conducted under greenhouse conditions to evaluate the influence of soil K levels on root growth and nutrient uptake of four upland rice cultivars. A completely randomized experimental design, in a 4x4 factorial scheme (4 levels of K: 20, 40, 80, and 160 mg dm-3; 4 cultivars: Caiapó, BRS-Primavera, IAC-202, and Maravilha) was used, with four replications. Based on regression equations, the highest values of root length density would be found with 136 mg dm-3 of K. The root diameter and dry matter, shoot dry matter and shoot K concentration increased linearly with the increasing K rates. The shoot K concentration of the upland rice cultivars did not differ. The increased level of K in the soil reduced the shoot Ca concentration of intermediate and modern cultivars, and the shoot Mg concentration of all cultivars. The potassium fertilization increased the plant growth, but the magnitude of this effect varied according to the cultivar.

Content of far-red light in supplemental light modified characteristics related to drought tolerance and post-planting growth in Scots pine seedlings

Light quality affects the morphological development of a plant. The aim of the experiment was to examine the effect of far-red radiation (FR, 700–800nm) on growth, morphology, and root growth capacity (RGC) in Scots pine seedlings originating from latitudes 61°N and 67°N, and on gas exchange and subsequent field growth in seedlings originating from 61°N. The seedlings were grown in a greenhouse under natural daylight, receiving either FR-rich (+FR) or FR-deficient (−FR) supplemental light for 20h per day, provided using light-emitting diodes (LEDs). The seedlings were outplanted and grown in the field for two growing seasons. Growth under +FR hastened terminal bud formation in seedings originating from 61°N, and delayed it in seedlings from 67°N. At the end of the growth period under the LEDs, +FR seedlings were dominated by secondary needles and had a higher needle mass than −FR seedlings, whose foliage comprised mainly primary needles. −FR improved root growth capacity in the seedlings originating from 61°N, but not in seedlings originating from 67°N. In seedlings from 61°N, photosynthesis (Pn) at the needle area level was higher and stomatal conductance lower under −FR, resulting in higher water use efficiency than under +FR. Due to the greater amount of photosynthetic tissue under +FR, the whole-seedling-level Pn was higher under +FR. Height and diameter growth were greater in −FR seedlings than in +FR seedlings during the second growing season. Growth habit and growth rate of Scots pine seedlings can be manipulated by controlling the FR content in the supplemental light, enabling production of seedlings targeted for outplanting on sites with specific characteristics.

Morphology, development, and transplant potential of Prunus aviumand Cornus sanguinea seedlings growing under different LED lights

The objective of the present study was to investigate the impact of light-emitting diodes (LEDs) on the morphological and developmental characteristics of wild cherry (Prunus avium) and common dogwood (Cornus sanguinea). The LEDs used were L20AP67 (moderate blue, red and far-red, high green), AP673L (moderate blue, high red), G2 (low blue, high red and far-red), AP67 (moderate blue, red and far-red), and NS1 (high blue and green, low red, high red:far-red, 1% ultraviolet). Fluorescent light [FL (high blue and green, low red)] tubes served as the control treatment. The growth rate and subsequently the shoot height of Cornus sanguinea were greater under FL. Root length of Prunus avium was longer under NS1. Prunus avium produced more biomass under NS1 and AP67, while Cornus sanguinea was favored under G2 and AP67. Greater root:shoot ratio was found under NS1 for Prunus avium and under NS1, AP67, G2, and AP673L for Cornus sanguinea. Root growth capacity (RGC) was also assessed in order to evaluate the transplanting response. RGC of Prunus avium and Cornus sanguinea was favored after precultivation under G2 and AP67, and under NS1 and AP67, respectively. Our study demonstrated that LEDs were more efficient in promoting a number of morphological characteristics than conventional FL in Prunus avium and Cornus sanguinea.

Comparative response of two wucai (Brassica campestris L.) genotypes to heat stress on antioxidative system and cell ultrastructure in root

The effects on root growth, root antioxidant capacity, and cellular ultrastructure were investigated using two wucai genotypes (heat-tolerant WS-1 and heat-sensitive WS-6) under heat stress (40/30 °C) for 5 days. Heat stress caused decreases in root biomass, relative water content (RWC), root vigor, and root traits of two wucai genotypes. In addition, it resulted in reactive oxygen species (ROS) accumulation and increased hydrogen peroxide (H2O2) content, superoxide anion (O2 −) formation rate, and malondialdehyde (MDA) content, but the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were inhibited to different extents in two genotype wucai roots. However, these data indicated that the decline extent of WS-1 (heat tolerant) in root growth and antioxidant capacity was significantly lower than that of WS-6 (Heat sensitive). Microscopic analyses revealed that WS-1 (heat tolerant) showed a better cellular shape than WS-6 under heat stress and slightly oxidative damage; nuclear and mitochondria in WS-1 were of a better intact shape and clear bilayer membrane. Most importantly, the thicker root cell wall in heat-tolerant wucai genotype responding to heat stress was first reported. These results suggested that the ability of heat-tolerant wucai genotype to minimize the heat stress depended upon the higher self-regulation capacity and effectiveness of the antioxidant metabolism.

Artificial LED lighting enhances growth characteristics and total phenolic content of Ocimum basilicum, but variably affects transplant success

The morphological and phytochemical characteristics of two Ocimum basilicum cultivars (Lettuce Leaf, and Red Rubin-mountain Athos hybrid) under artificial lighting were investigated. Four LED light treatments [AP673L (high red and high red:far-red), G2 (high red and low red:far-red), AP67 (moderate blue and red and low red:far-red), and NS1 (high blue and green, high red:far-red and 1% ultraviolet)] with different colors mixing UV, blue, green, red and far-red, and fluorescent tubes (FL, high blue, green and red:far-red) as Control were used in the growth chambers for 28 days under PPFD of 200±20μmolm−2s−1 for all treatments at plant height. G2, Control and AP67 treatments for Lettuce Leaf, and G2 for Red Rubin hybrid had higher growth rate. Roots of Lettuce Leaf were significantly longer under AP673L compared to NS1, while Red Rubin hybrid showed no significant differences. Total biomass was significantly greater under NS1, AP67 and G2 compared to the Control, for both cultivars. For both Lettuce Leaf and Red Rubin hybrid, root:shoot ratio (R/S) was favored under NS1, whereas the Control had the lowest impact. Leaf area of both cultivars was greater under the Control. Root growth capacity evaluation was also assessed. Seedlings of Lettuce Leaf cultivated under the effect of the Control and AP673L, and seedlings of Red Rubin hybrid grown under AP673L (mainly) quickly developed new root system. This could offer the advantage of fast exploitation of larger soil volume after transplanting. Total phenolic content of Lettuce Leaf was significantly higher under NS1 compared to the rest of the treatments, while in Red Rubin hybrid, NS1 had significantly higher total phenolic content compared to the Control and G2. Our study demonstrates that LEDs variably affected growth characteristics and increased total phenolic content compared to conventional fluorescent light for these two O. basilicum cultivars.