The inter-shaft bearing is a critical component in dual rotors aero-engine. Due to the complex and severe loads caused by the interactive excitation of HP and LP rotors during operation, the inter-shaft bearing is prone to damage accumulation and failure. Focusing on a typical failure of inter-shaft bearing inner ring fragmentation and cannot be spliced together completely in high thrust-weight ratio turbofan engine, this paper conducted morphological investigation, fractographic analysis, vibration signal processing, finite element numerical simulation, and fully studied the failure mechanism of this failure.The results indicated that: there exists an uncoordinated resonance speed of the HP rotor in close proximity to the operating speed. This puts the rotor in non-synchronous procession state, accompanied by the uncoordinated angular displacement of the inner and outer rings, which resulting in a lager rolling dynamic load in bearing inner ring. This further leads to high tangential impact load at the root fillet of the anti-rotation pin, causing significant stress concentration, and eventually leads to the generation of initial cracks. These cracks persist and propagate into fatigue oblique fracture over time. After oblique fracture, the modal frequencies of the inner ring become more dispersed. Under the rolling dynamic load (traveling wave load), nodal diameter vibration occurs, causing high stress vibration fatigue damage, and ultimately leading to multiple-point simultaneous fragmentation.The investigation suggests that reducing the stress levels at the root fillet of the anti-rotation pin of the inner ring is an effective approach to prevent bearing failure. This can be achieved through two methods: one is optimizing the rotor structure to decrease the rolling dynamic load on the inner ring, the other is eliminating the anti-rotation pin and appropriately reducing the tightening torque of the compression nut and the interference between the bearing inner ring and the HP turbine rear journal.