Objective To explore the application effect of PDCA cycle combined with standardized operation procedure in periodic cleaning of objects surfaces of clean operating room, so as to provide reference for management and control of surgical infection. Methods PDCA cycle was applied to analyze the periodic cleaning conditions of 336 surgical rooms in the operating room from July to December 2016.The results showed that the clearance rate of surface fluorescence labeling was 75.30%, the average time of periodic cleaning was 3.5 hours per operation room, the ratio of periodic cleaning personnel to room was 57.14%, the average defect rate of surface cleaning was 37.80%, the quality control rate was 27.38%, etc.Improvement measures were formulated: the cleaning table of periodic objects between clean operations was formulated to clarify the contents of periodic cleaning, and standardized operation procedure manual and video were developed accordingly for the training of general practitioners, and the responsibility was transferred to people.Comparison was made between January and June 2017 after the intervention. Results Compared with before intervention, the clearance rate of fluorescent label monitoring on the interoperative table was increased to 93.75%, the average time of periodic cleaning was reduced to 2.2 hours, the room ratio of periodic cleaning personnel was 28.57%, the defect rate of surface cleaning was 3.2%, and the quality control rate was 7.8%. All the differences were statistically significant (P<0.05). Conclusion PDCA cycle combined with standardized operation procedure can significantly improve the qualified rate of periodic cleaning of the surface of the clean operating room.It can reduce the defect rate of surface cleaning, shorten the cleaning time, reduce labor cost, improve work efficiency, enhance the quality of infection control in operating room, and it is worth popularizing and applying in clinic. Key words: Plan-Do-Check-Action; Standardized operation procedure; Operating room; Object surfaces clean; Infection control