Background and Aim: Spiritual leadership positively and significantly effects on spiritual well-being and organizational-personnel performance variables, through drawing faith in a vision and culture of excellence. The purpose of this study was to investigate the role of spiritual leadership and professional ethics in the organizational commitment of health care workers. Materials and Methods: In this descriptive-correlational study, the statistical population consisted of all health care workers who work in health facilities of the Urmia, Iran, in 2017. The sample size was estimated 240 people by Morgan table. Stratified random sampling method, and Fry’s Spiritual Leadership, Ghasemzadeh’s professional ethics and organizational Allen and Meyer ’ s commitment Questionnaire were used to collect data. SPSS 22 and Amos statistical soft wares were used to analyze the data. Findings: The direct effect of the spiritual leadership on professional ethics (0.37) and organizational commitment of the health care workers was significant (p=0.01). The direct effect of the spiritual leadership on organizational commitment of health care workers (0.69) also was significant (p=0.01). The indirect effect of the spiritual leadership on organizational commitment of health care workers also was significant (p=0.01). The fitting of the predictive model of the research variables was relatively good (62%) and suggests the alignment of the fitted model with the theoretical community of research. Conclusion: Based on the findings, it can be concluded that spiritual leadership influences on the organizational commitment of health care workers with the mediation role of professional ethics. Please cite this article as: Zahed Babelan A, Khaleg Khah A, Kazemi S, Gharibzadeh R. The Role of Spiritual Leadership and Professional Ethics in Organizational Commitment of Health Care Workers. Bioethics Journal 2017; 7(26): 23-30.