Granites containing abundant dioritic enclaves are widespread in the Songnen–Zhangguangcai Range Massif (SZRM). However, the role of mantle- and crust-derived magmas in the petrogenesis of the dioritic enclaves is debated. We present new zircon U–Pb–Hf–O isotopic compositions, whole-rock geochemistry, mineral geochemistry, and Sr–Nd isotopic compositions of hornblendites and dioritic enclaves and their host granites to elucidate their origins and investigate crust–mantle hybridization. Zircon U–Pb dating suggests that these intrusive rocks were emplaced during the Early Jurassic (197–186 Ma). The geochemical characteristics of hornblendites are similar to those of the Early Jurassic mafic–ultramafic rocks in the Lesser Xing’an–Zhangguangcai Range. They yield low (87Sr/86Sr)i ratios (0.704266–0.704324), high εNd(t) values (3.87–3.91), and are enriched in Ba, Sr, and K and depleted in Nb, Ta, and Ti, suggesting that they were derived from the partial melting of mantle wedge metasomatized by sediment melt. The host granites have high SiO2 (>65.12 wt%) and low MgO (<1.92 wt%) and Al2O3 (<16.23 wt%) contents and are to metaluminous–peraluminous high-K calc-alkaline rocks. In addition, they yield positive zircon εHf(t) values (3.42–7.58), zircon δ18O values (4.87‰–5.85‰) and whole-rock εNd(t) values (0.10–0.44), suggesting that the granitic magma originated from the partial melting of continental crust with the involvement of mantle-derived melts. The field and petrographic observations and geochemical analyses indicate that the enclaves were derived from magma mixing and mingling. The dioritic enclaves contain K-feldspar megacrysts and back-veins, and plagioclase crystals within them are reversely zoned and have ocellar textures. The weighted mean ages of the host granites (186 ± 3 and 188 ± 2.3 Ma) are similar to those of the dioritic enclaves (186 ± 1.8 and 188 ± 1.1 Ma). The major element contents of the intrusive rock are linearly correlated with the SiO2 contents, and the whole-rock geochemical and isotopic characteristics of the hornblendites and host granites show that they are the mafic and felsic end-members, respectively, involved in the magma mixing. Therefore, we propose that underplating by mantle-derived magma likely triggered partial melting of the continental crust to produce felsic magma, and the dioritic enclaves were formed by the mixing and mingling of mantle- and crust-derived magma alongside melting, assimilation, storage, and homogenization. This suggest that the Mudanjiang Oceanic crust was subducted westward beneath the SZRM during the Early Jurassic.