Role In Flowering Research Articles

THE SYMBOLIC AND SPIRITUAL ROLE OF FLOWERS IN HINDU RELIGIOUS RITUALS IN BALI: A QUALITATIVE ANALYSIS

Humans' inability to understand God in metaphysical form often gives rise to the need to use symbolic or representational means in an effort to understand or describe a sublime and unattainable existence, one of which is a vital object as a symbol of God for the Balinese Hindu community is the existence of flowers in Every offering is considered as a condition that increases aesthetic passion or a condition within the spirit of human relationship with God of Flowers in Hindu religious practices in Bali. The research uses qualitative methods with a descriptive approach used to analyze and describe data from literature studies, strengthening understanding about the presence of flowers in rituals. The results of the research are divided into three discussion points (1) The Significance of Flowers in Hindu Religious Rites in Bali (2) Flowers as a Medium of Offerings (3) Spirit and Blessing: The Role of Flowers in Spirituality and the Human Environment, highlighting the importance of flowers in conveying the sincerity of the human heart in religious ceremonies, enrich spirituality, and deepen understanding of God's existence through religious symbols. Based on this, this research attempts to examine in depth the existence of flowers as a means of ritual through depiction or symbolization which allows humans to associate complex and abstract concepts such as God with something more concrete or visible, such as images, symbols, or rituals. This facilitates easier understanding and helps in forming an emotional, spiritual, or even intellectual bond with the concept of God.

Uncoupling FRUITFULL's functions through modification of a protein motif identified by co-ortholog analysis.

Many plant transcription factors (TFs) are multifunctional and regulate growth and development in more than one tissue. These TFs can generally associate with different protein partners depending on the tissue type, thereby regulating tissue-specific target gene sets. However, how interaction specificity is ensured is still largely unclear. Here, we examine protein-protein interaction specificity using subfunctionalized co-orthologs of the FRUITFULL (FUL) subfamily of MADS-domain TFs. In Arabidopsis, FUL is multifunctional, playing important roles in flowering and fruiting, whereas these functions have partially been divided in the tomato co-orthologs FUL1 and FUL2. By linking protein sequence and function, we discovered a key amino acid motif that determines interaction specificity of MADS-domain TFs, which in Arabidopsis FUL determines the interaction with AGAMOUS and SEPALLATA proteins, linked to the regulation of a subset of targets. This insight offers great opportunities to dissect the biological functions of multifunctional MADS TFs.

“Blooming” of litter-mixing effects: the role of flower and leaf litter interactions on decomposition in terrestrial and aquatic ecosystems

Abstract. The diversity effect on decomposition, through the litter-mixing effects plays a central role in determining the nutrient and carbon dynamics in ecosystems. However, the litter-mixing effects are centered on a leaf litter perspective. Important aspects related to intraspecific interaction and biomass concentration are rarely evaluated, even though they could be essential to determine the litter decomposition dynamics. To our knowledge, we introduced a new perspective to evaluate whether and how the interaction between flower and leaf litter affects the occurrence, direction, and magnitude of litter-mixing effects in terrestrial and aquatic ecosystems. We performed laboratory experiments using flower and leaf litter from the yellow trumpet tree Tabebuia aurea (Silva Manso) Benth. and Hook. f. ex. S. Moore as a model. To obtain realistic results, we manipulated various scenarios of flower : leaf litter biomass proportion and measured 13 functional traits. Litter-mixing effects were consistent in both aquatic and terrestrial environments, with faster decomposition of both litter types in mixtures compared to their monocultures (synergistic effects). Litter-mixing effects were stronger in the terrestrial environment and at higher flower : leaf litter biomass proportions. Our results indicate that synergistic outcomes are mainly associated with complementary effects. Flower litter had a higher concentration of labile C compounds, N, P, and K and lower lignin concentrations, representing a labile litter, while leaf litter had a higher concentration of lignin, Ca, Mg, and Na, representing a refractory litter. Our results demonstrate the importance of litter-mixing effects between flower and leaf litter via complementary effects. These results shed light on the secondary consequences of flower litter on decomposition, suggesting that species with high reproductive investment in flower biomass may play an important role in the nutrient and carbon recycling of diverse plant communities, exerting a pivotal role in biogeochemical dynamics.

Effect of Foliar Feeding of Plant Growth Regulator and Nutrients on Phenological Attributes of Guava (Psidium guajava L.) cv Apple Colour

Guava, (Psidium guajava L.), small tropical tree or shrub of the family Myrtaceae, cultivated for its edible fruits. Guava trees are native to tropical America and are grown in tropical and subtropical areas worldwide. Nutrients and PGR play a crucial role in flowering and fruit production and their deficiency can significantly impact the yield, productivity, and quality of fruits. Therefore, keeping these points in view, the present investigation was carried out on a 10-year-old guava plant in a high-density plantation (3x3) at the Fruit Research Station Imaliya, Department of Horticulture, J.N.K.V.V., Jabalpur (M.P.) during the mrig-bahar season of 2022-23. The study employed a Factorial Randomised Block Design (FRBD) with 20 treatment combinations involving Plant Growth Regulators (PGR) like salicylic acid and various nutrients, replicated three times. Results indicated that the combination of salicylic acid (200 ppm) + borax (0.5%) significantly influenced several phenological parameters. This treatment resulted in the earliest flower bud initiation (18.33 days) after foliar application, the highest average number of flower buds per shoot (7.05), minimum days to 50% flowering (33.67 days), maximum average number of flowers per shoot (6.95), the highest fruit set percentage (92.18%), the highest fruit retention percentage (81.23%), and the lowest fruit drop percentage (18.77%). The application of salicylic acid at 200 ppm in conjunction with borax at 0.5% (S2 M4) followed by salicylic acid at 300 ppm with borax at 0.5% (S3 M4) demonstrated exceptional efficacy in significantly optimizing phenological parameters when compared to the control.

Rice OBF binding protein 4 (OsOBP4) participates in flowering and regulates salt stress tolerance in Arabidopsis

DNA-binding with one finger (DOF) transcription factors (TFs) play an important role in gene regulation for the proper growth and development of plants. Ocs-element binding factor (OBF) binding protein 4 (OBP4), an essential member of the DOF TF family, regulates flowering and abiotic stress. However, the functions of OBP4 in rice remain obscure, which limits its potential use in molecular breeding. In this study, rice OBP4 (OsOBP4) was functionally characterized and its role in flowering and abiotic stress was verified. Phylogenetic analysis revealed that OsOBP4 is homologous to OBP4 in Arabidopsis. OsOBP4 is modulated by salt, osmotic, and abscisic acid (ABA) stresses. Additionally, ectopic expression of OsOBP4 complements the phenotype of the Atobp4 mutation by enhancing root development and protecting the photosynthesis system. Under salt stress and short-day conditions, OsOBP4 regulated floral development by interacting with AGL7, the upstream component for floral meristem initiation. These results provide evidence for the role of OsOBP4 in multiple responses to salt stress and suggest that OsOBP4 could be used as a potential candidate gene to improve tolerance to salt stress.

MiCOL6, MiCOL7A and MiCOL7B isolated from mango regulate flowering and stress response in transgenic Arabidopsis.

The CONSTANS/CONSTANS-Like (CO/COL) family has been shown to play important roles in flowering, stress tolerance, fruit development and ripening in higher plants. In this study, three COL genes, MiCOL6, MiCOL7A and MiCOL7B, which each contain only one CCT domain, were isolated from mango (Mangifera indica), and their functions were investigated. MiCOL7A and MiCOL7B were expressed mainly at 20 days after flowering (DAF), and all three genes were highly expressed during the flowering induction period. The expression levels of the three genes were affected by light conditions, but only MiCOL6 exhibited a clear circadian rhythm. Overexpression of MiCOL6 promoted earlier flowering, while overexpression of MiCOL7A or MiCOL7B delayed flowering compared to that in the control lines of Arabidopsis thaliana under long-day (LD) and short-day (SD) conditions. Overexpressing MiCOL6, MiCOL7A or MiCOL7B in transgenic plants increased superoxide dismutase (SOD) and proline levels, decreased malondialdehyde (MAD) levels, and improved survival under drought and salt stress. In addition, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses showed that the MiCOL6, MiCOL7A and MiCOL7B proteins interact with several stress- and flower-related proteins. This work demonstrates the functions of MiCOL6, MiCOL7A and MiCOL7B and provides a foundation for further research on the role of mango COL genes in flowering regulation and the abiotic stress response.

ACC SYNTHASE4 inhibits gibberellin biosynthesis and FLOWERING LOCUS T expression during citrus flowering.

Flowering is an essential process in fruit trees. Flower number and timing have a substantial impact on the yield and maturity of fruit. Ethylene and gibberellin (GA) play vital roles in flowering, but the mechanism of coordinated regulation of flowering in woody plants by GA and ethylene is still unclear. In this study, a lemon (Citrus limon L. Burm) 1-aminocyclopropane-1-carboxylic acid synthase gene (CiACS4) was overexpressed in Nicotiana tabacum and resulted in late flowering and increased flower number. Further transformation of citrus revealed that ethylene and starch content increased, and soluble sugar content decreased in 35S:CiACS4 lemon. Inhibition of CiACS4 in lemon resulted in effects opposite to that of 35S:CiACS4 in transgenic plants. Overexpression of the CiACS4-interacting protein ETHYLENE RESPONSE FACTOR3 (CiERF3) in N. tabacum resulted in delayed flowering and more flowers. Further experiments revealed that the CiACS4-CiERF3 complex can bind the promoters of FLOWERING LOCUS T (CiFT) and GOLDEN2-LIKE (CiFE) and suppress their expression. Moreover, overexpression of CiFE in N. tabacum led to early flowering and decreased flowers, and ethylene, starch, and soluble sugar contents were opposite to those in 35S:CiACS4 transgenic plants. Interestingly, CiFE also bound the promoter of CiFT. Additionally, GA3 and 1-aminocyclopropanecarboxylic acid (ACC) treatments delayed flowering in adult citrus, and treatment with GA and ethylene inhibitors increased flower number. ACC treatment also inhibited the expression of CiFT and CiFE. This study provides a theoretical basis for the application of ethylene to regulate flower number and mitigate the impacts of extreme weather on citrus yield due to delayed flowering.

An atypical heterotrimeric Gα and its interactome suggest an extra-large role in overcoming abiotic and biotic stress.

Canonical heterotrimeric G-proteins (G-proteins) are comprised of Gα, Gβ, and Gγ subunits. G-proteins regulate multiple crucial plant growth and development processes, incorporating environmental responses. Besides Gα, Gβ and Gγ, the discovery of atypical Gα subunits termed as extra-large G-proteins or extra-large GTP-binding proteins (XLGs) makes G-protein signaling unique in plants. The C-terminus of XLG shares similarities with the canonical Gα subunits; the N-terminus harbors a nuclear localization signal (NLS) and is rich in cysteine. The earlier explorations suggest XLG's role in flowering, the development of embryos and seedlings, root morphogenesis, stamen development, cytokinin-induced development, stomatal opening and regulation of rice grain filling. The XLGs are also known to initiate signaling cascades that prime plants against a variety of abiotic and biotic stresses. They are also engaged in controlling several agronomic parameters such as rice panicle length, grain filling, grain size, and biomass, highlighting their potential contribution to crop improvement. The present review explores the remarkable properties of non-canonical Gα subunits (XLGs) and reflects on the various developmental, abiotic and biotic stress signaling pathways controlled by them. Moreover, the bottleneck dilemma of how a tiny handful of XLGs control a multiplicity of stress-responsive activities is partially resolved in this review by addressing the interaction of XLGs with different interacting proteins. XLG proteins presented in this review can be exploited to gain access to highly productive and stress-tolerant plants.

The effect of phytohormones on the flowering of plants

During the development of angiosperms, one of the most critical stages of plants is the transition from vegetative to reproductive stage, and successfully producing seeds is necessary. Plants have developed a complex signaling pathway to recognize and combine endogenous and environmental signals. Plant growth regulators (PGRs) play a role in regulating flower growth on shoots. Physiological and biochemical processes work together to differentiate and produce flower buds. The impact of PGRs on floral bud differentiation has been the subject of several publications in recent years. In addition, the dynamic variations in gibberellin (GA), auxin, and cytokinin levels in buds and the hormonal-related signatures in gene regulatory networks indicate a crucial function for these hormones during floral bud development in plants. Especially the flowering hormone GA has a key role in regulating the activities related to flowering genes as well as controlling the activity of the DELLA protein. Abscisic acid (ABA) and ethylene (ET) have an inhibitory role in flowering but in some cases stimulate flowering depending on environmental conditions. This study aims to understand the regulation of phytohormones on flowering of plants and its effects on plant development during the flowering stage.

Novel roles of HSFs and HSPs, other than relating to heat stress, in temperature-mediated flowering.

The thermotolerant ability of heat shock factors (HSFs) and heat shock proteins (HSPs) in plants has been shown. Recently, focus has been on their function in plant growth and development under non-stress conditions. Their role in flowering has been suggested given that lower levels of HSF/HSPs resulted in altered flowering in Arabidopsis. Genetic and molecular studies of Arabidopsis HSF/HSP mutants advocated an association with temperature-mediated regulation of flowering, but the fundamental genetic mechanism behind this phenomenon remains obscure. Here we outline plausible integration between HSFs/HSPs and temperature-dependent pathways in plants regulating flowering. Moreover, we discuss how similar pathways can be present in thermoperiodic geophytic plants that require ambient high temperatures for flowering induction.

Comparative Genome-Wide Analysis of MicroRNAs and Their Target Genes in Roots of Contrasting Indica Rice Cultivars under Reproductive-Stage Drought.

Recurrent occurrence of drought stress in varying intensity has become a common phenomenon in the present era of global climate change, which not only causes severe yield losses but also challenges the cultivation of rice. This raises serious concerns for sustainable food production and global food security. The root of a plant is primarily responsible to perceive drought stress and acquire sufficient water for the survival/optimal growth of the plant under extreme climatic conditions. Earlier studies reported the involvement/important roles of microRNAs (miRNAs) in plants' responses to environmental/abiotic stresses. A number (738) of miRNAs is known to be expressed in different tissues under varying environmental conditions in rice, but our understanding of the role, mode of action, and target genes of the miRNAs are still elusive. Using contrasting rice [IR-64 (reproductive-stage drought sensitive) and N-22 (drought-tolerant)] cultivars, imposed with terminal (reproductive-stage) drought stress, we demonstrate differential expression of 270 known and 91 novel miRNAs in roots of the contrasting rice cultivars in response to the stress. Among the known miRNAs, osamiR812, osamiR166, osamiR156, osamiR167, and osamiR396 were the most differentially expressed miRNAs between the rice cultivars. In the root of N-22, 18 known and 12 novel miRNAs were observed to be exclusively expressed, while only two known (zero novels) miRNAs were exclusively expressed in the roots of IR-64. The majority of the target gene(s) of the miRNAs were drought-responsive transcription factors playing important roles in flower, grain development, auxin signaling, root development, and phytohormone-crosstalk. The novel miRNAs identified in this study may serve as good candidates for the genetic improvement of rice for terminal drought stress towards developing climate-smart rice for sustainable food production.

Genome-Wide Identification, Characterization and Expression Profiling of the CONSTANS-like Genes in Potato (Solanum tuberosum L.).

CONSTANS-like (COL) genes play important regulatory roles in flowering, tuber formation and the development of the potato (Solanum tuberosum L.). However, the COL gene family in S. tuberosum has not been systematically identified, restricting our knowledge of the function of these genes in S. tuberosum. In our study, we identified 14 COL genes, which were unequally distributed among eight chromosomes. These genes were classified into three groups based on differences in gene structure characteristics. The COL proteins of S. tuberosum and Solanum lycopersicum were closely related and showed high levels of similarity in a phylogenetic tree. Gene and protein structure analysis revealed similarities in the exon-intron structure and length, as well as the motif structure of COL proteins in the same subgroup. We identified 17 orthologous COL gene pairs between S. tuberosum and S. lycopersicum. Selection pressure analysis showed that the evolution rate of COL homologs is controlled by purification selection in Arabidopsis, S. tuberosum and S. lycopersicum. StCOL genes showed different tissue-specific expression patterns. StCOL5 and StCOL8 were highly expressed specifically in the leaves of plantlets. StCOL6, StCOL10 and StCOL14 were highly expressed in flowers. Tissue-specific expression characteristics suggest a functional differentiation of StCOL genes during evolution. Cis-element analysis revealed that the StCOL promoters contain several regulatory elements for hormone, light and stress signals. Our results provide a theoretical basis for the understanding of the in-depth mechanism of COL genes in regulating the flowering time and tuber development in S. tuberosum.

Spatio-temporal analysis of strawberry architecture: insights into the control of branching and inflorescence complexity.

Plant architecture plays a major role in flowering and therefore in crop yield. Attempts to visualize and analyse strawberry plant architecture have been few to date. Here, we developed open-source software combining two- and three-dimensional representations of plant development over time along with statistical methods to explore the variability in spatio-temporal development of plant architecture in cultivated strawberry. We applied this software to six seasonal strawberry varieties whose plants were exhaustively described monthly at the node scale. Results showed that the architectural pattern of the strawberry plant is characterized by a decrease of the module complexity between the zeroth-order module (primary crown) and higher-order modules (lateral branch crowns and extension crowns). Furthermore, for each variety, we could identify traits with a central role in determining yield, such as date of appearance and number of branches. By modeling the spatial organization of axillary meristem fate on the zeroth-order module using a hidden hybrid Markov/semi-Markov mathematical model, we further identified three zones with different probabilities of production of branch crowns, dormant buds, or stolons. This open-source software will be of value to the scientific community and breeders in studying the influence of environmental and genetic cues on strawberry architecture and yield.

The role of flowers in the personalization of Christian funerals in Denmark

Flowers are a common element in Danish funerals. Drawing on fieldnotes, interviews and survey data on funeral practices in the Evangelical Lutheran Church of Denmark as well as theories of ritualization, meaning-making and practices, this article shows that flowers are not only a sine qua non in the funerals but are also used to make them more personal and to produce and reproduce social relations. Additionally, flowers are material objects and acquire their social meaning in the right ceremonial context. Outside this context they have no inherent meaning and might even obstruct the ceremony because, as physical objects, they have to be put somewhere in ceremonial space. Paradoxically, flowers are ubiquitous yet invisible.

The NAC transcription factors play core roles in flowering and ripening fundamental to fruit yield and quality.

Fruits are derived from flowers and play an important role in human food, nutrition, and health. In general, flowers determine the crop yield, and ripening affects the fruit quality. Although transcription factors (TFs) only account for a small part of plant transcriptomes, they control the global gene expression and regulation. The plant-specific NAC (NAM, ATAF, and CUC) TFs constitute a large family evolving concurrently with the transition of both aquatic-to-terrestrial plants and vegetative-to-reproductive growth. Thus, NACs play an important role in fruit yield and quality by determining shoot apical meristem (SAM) inflorescence and controlling ripening. The present review focuses on the various properties of NACs together with their function and regulation in flower formation and fruit ripening. Hitherto, we have a better understanding of the molecular mechanisms of NACs in ripening through abscisic acid (ABA) and ethylene (ETH), but how NACs regulate the expression of the inflorescence formation-related genes is largely unknown. In the future, we should focus on the analysis of NAC redundancy and identify the pivotal regulators of flowering and ripening. NACs are potentially vital manipulation targets for improving fruit quantity and quality.

Identification and Analysis of Phosphatidylethanolamine-Binding Protein Family Genes in the Hangzhou White Chrysanthemum (Chrysanthemum morifolium Ramat)

The Hangzhou White Chrysanthemum (Chrysanthemum morifolium Ramat) is one of the “Zhejiang eight flavors” in traditional Chinese medicine. The phosphatidylethanolamine-binding protein (PEBP) plays an important role in flowering and floral organ development. Even so, the biological role of PEBPs in the Hangzhou White Chrysanthemum has not been studied, which attracted us. Here, nine CmPEBP genes that contain the PF01161 domain were identified in the Hangzhou White Chrysanthemum for the first time, and their biological role in flowering was preliminarily studied. A phylogenetic analysis classified the CmPEBP genes into three subfamilies: MFT-like, TFL-like, and FT-like genes. The differential expression analysis was performed under different tissues and different stressors using qRT-PCR. It showed that each CmPEBP displayed tissue-specific expression patterns. Expression patterns in response to different temperatures and hormone stressors were investigated. They were finally demonstrated to be differentially expressed. TFL-like gene expression, which delayed reproductive growth, was upregulated under heat stress. Conversely, FT-like gene expression was upregulated under low temperatures. CmFT1 expression could be inhibited by GA (gibberellin), 6-BA (benzylaminopurine), ET (ethylene), and MeSA (methyl salicylate) but could be activated by IAA (indole-3-aceticacid), ABA (abscisic acid), and SA (salicylic acid) in the dark, whereas CmFT2 and CmFT3 expression levels were upregulated by ET, MeJA (methyl jasmonate), and ABA but were downregulated by 6-BA, SA, and MeSA. GA, IAA, SA, and MeSA inhibited CmTFL gene expression under light and dark treatments. Further research on CmPEBP genes in the Hangzhou White Chrysanthemum could better determine their roles in flowering and floral organ development, especially in response to the prolonged spraying of exogenous hormones.

A Tea Plant (Camellia sinensis) FLOWERING LOCUS C-like Gene, CsFLC1, Is Correlated to Bud Dormancy and Triggers Early Flowering in Arabidopsis

Flowering and bud dormancy are crucial stages in the life cycle of perennial angiosperms in temperate climates. MADS-box family genes are involved in many plant growth and development processes. Here, we identified three MADS-box genes in tea plant belonging to the FLOWERING LOCUS C (CsFLC) family. We monitored CsFLC1 transcription throughout the year and found that CsFLC1 was expressed at a higher level during the winter bud dormancy and flowering phases. To clarify the function of CsFLC1, we developed transgenic Arabidopsis thaliana plants heterologously expressing 35S::CsFLC1. These lines bolted and bloomed earlier than the WT (Col-0), and the seed germination rate was inversely proportional to the increased CsFLC1 expression level. The RNA-seq of 35S::CsFLC1 transgenic Arabidopsis showed that many genes responding to ageing, flower development and leaf senescence were affected, and phytohormone-related pathways were especially enriched. According to the results of hormone content detection and RNA transcript level analysis, CsFLC1 controls flowering time possibly by regulating SOC1, AGL42, SEP3 and AP3 and hormone signaling, accumulation and metabolism. This is the first time a study has identified FLC-like genes and characterized CsFLC1 in tea plant. Our results suggest that CsFLC1 might play dual roles in flowering and winter bud dormancy and provide new insight into the molecular mechanisms of FLC in tea plants as well as other plant species.

Genome-wide identification of the auxin response factor (ARF) gene family in Magnolia sieboldii and functional analysis of MsARF5.

Auxin plays an essential role in flowering, embryonic development, seed dormancy, and germination. Auxin response factors (ARFs) are plant-specific key transcriptional factors in mediating the gene expression network of auxin signaling. Although ARFs in model plants such as Arabidopsis had been well characterized, their identities and potential roles in non-model plants are less studied. Here, we performed genome-wide identification of ARFs in Magnolia sieboldii K. Koch, a primitive species with high taxonomic importance and medicinal values. We found 25 ARF genes in M. sieboldii, which were widely distributed across multiple chromosomes. Based on sequence similarity, the encoded proteins could be either transcriptional repressors or activators. Gene expression analysis showed a dynamic pattern for many ARFs including MsARF5 during seed germination. In addition, overexpressing of MsARF5 showed that it restores many developmental defects in the Arabidopsis mutant. Moreover, two phenotypically distinct transgenic Arabidopsis lines were obtained, indicating a link between gene expression levels and developmental phenotypes. Taken together, we provided a systematic investigation of the ARF gene family in M. sieboldii and revealed an important role of MsARF5 in mediating auxin signaling.

Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato

Plant organ abscission is a common phenomenon that occurs at a specific position called the abscission zone (AZ). The differentiation and development of the pedicel AZ play important roles in flower and fruit abscission, which are of great significance for abscission in tomatoes before harvest. Previous studies have reported some genes involved in AZ differentiation; however, the genes regulating pedicel AZ cell development in tomatoes after AZ differentiation remain poorly understood. In this study, transcriptome analyses of tomato pedicel AZ samples were performed at 0, 5, 15, and 30 days post-anthesis (DPA). Pedicel AZ growth was mainly observed before 15 DPA. A principal component analysis and a correlation analysis were carried out in order to compare the repeatability and reliability for different samples. We observed 38 up-regulated and 31 down-regulated genes that were significantly altered during 0 to 5 DPA, 5 to 15 DPA, and 0 to 15 DPA, which may play key roles in AZ cell enlargement. GO and KEGG enrichment analyses of the selected DEGs under all three different comparisons were conducted. Auxin-signaling-related genes were analyzed, as well as AUX/IAA, GH3, and small auxin up-regulated RNA (SAUR) gene expression patterns. The presented results provide information on pedicel AZ development, which might help in regulating flower or fruit pedicel abscission in tomato production facilities.

Analysis of expression and interaction proteins of BjuSPL10c in B. juncea

• BjuSPL10c was expressed at a high level in flowers and up-regulated at reproductive stage. • Seven potential interactive proteins (BjuB025567, BjuA041065, BjuB033332, BjuA045694, BjuA027365, BjuA031237 and BjuA005619) were screened from B.juncea yeast library of flower buds at squaring stage. • Six (BjuB025567, BjuA041065, BjuB033332, BjuA045694, BjuA031237 and BjuA005619) of those proteins interacted with BjuSPL10c in vivo. • All of these six genes expressing interactive proteins were down-regulated in flowers. Brassica juncea var. tumida Tsen et Lee (Tumorous stem mustard) is a specific vegetable in China. Its tumorous stem can be consumed as fresh vegetable or produced pickle. In practice, early-bolting happens in around 15%, which inhibits B. juncea industry development. SPL is a plant specific transcription factor, plays important roles in flowering and gibberellins (GA) signaling. qRT-PCR assay found that BjuSPL10c was expressed at a high level in flowers and reproductive stage. To explore interactive proteins, BjuSPL10c was used as bait, finally seven proteins were screened and they are BjuB025567, BjuA041065, BjuB033332, BjuA045694, BjuA027365, BjuA031237 and BjuA005619. Further study confirmed that six of those proteins interacted with BjuSPL10c in vivo. qRT-PCR was conducted to understand mRNA expression levels of these six proteins in different tissues and development stages. The results showed that compared with root, mRNAs for all of these six genes were down-regulated in flowers. For different developmental stages, BjuB025567 and BjuA005619 were up-regulated at vegetative stage, down-regulated at reproductive stage; BjuB033332, BjuA045694 and BjuA031237 were significantly up-regulated at both vegetative and reproductive stages; BjuA041065 was showed “down-up-down” expression pattern. Taking all results together, BjuB025567, BjuA041065, BjuB033332, BjuA045694 and BjuA031237 may participate in flowering time regulation through interacting with BjuSPL10c in B. juncea .