Biochar has been shown to enhance soil quality and agricultural yields. Previous studies about biochar's effect on soil properties mainly concentrated on the top 30 cm layer but less on the subsoils. Given the subsoil's active role in climate change mitigation and its significance for nutrient cycling and crop productivity, understanding biochar's effects at depth is crucial. This study explored the responses of soil organic carbon (SOC), total nitrogen, available potassium, available phosphorus, pH, and electrical conductivity to different doses of biochar addition (0, 10, 20, and 30 Mg/ha) over the 1 m depth of alkaline soil. Additionally, the impact of biochar on soil microbial community was assessed in the top 20 cm. Results demonstrated that biochar addition can increase SOC and improve soil properties in deep soil horizons. Specifically, a 30 Mg/ha biochar addition increased SOC by 1.2–10.1 Mg C/ha in the 10–40 cm layer and by 3 Mg C/ha in the 60–80 cm depth over two years. Additionally, biochar addition at this rate increased total nitrogen by 0.2–0.3 g N/kg in the 10–40 cm depth and elevated available potassium across the 1 m profile, with a maximum increment of 313 mg/kg in the surface 10 cm and a minimum of 97 mg/kg in the 40–60 cm depth. While biochar application did not increase available phosphorus, it resulted in a minor decrease in soil pH (<0.7 units) and a slight increase in electrical conductivity. Moreover, biochar addition did not significantly alter the soil microbial community. Our findings underscore the importance of considering subsoils when evaluating biochar's impact on soil properties. We suggest that subsoils should be considered when estimating the potential of cropland management for increasing soil carbon sequestration and improving soil conditions.