Rocky Shore Habitats Research Articles

Molecular population genetics and phylogeographic studies of Ligia exotica and Ligia cinerascens in East Asia

IntroductionSea slater, in the genus Ligia, is widespread in rocky shore habitats, and the taxon is easily isolated due to its limited dispersal capacity. Therefore, most Ligia species exhibit an allopatric distribution, but Ligia exotica and L. cinerascens exhibit an overlapping distribution distribution in East Asia. Previous studies on both species have confirmed the existence of highly divergent lineages based on 16S rRNA.MethodsIn the present study, 282 Ligia individuals were collected at ten, three, and three sites in South Korea, Japan, and Vietnam, respectively, and 41 haplotypes were observed based on 16S rRNA.Results and discussionThe results of phylogeny, phylogenetic network, and TCS network, Principal Coordinates Analysis, and four Molecular Species Delimitation Analyses revealed that six genetic lineages including L. cinerascens, Lineages N and S of L. exotica, Ligia sp. 1, sp.2 and sp.3 were present. The three genetic lineages, including L. cinerascens, Lineage N of L. exotica, and Lineage S of L. exotica, were also identified in the phylogeny based on a nuclear gene of the sodium–potassium ATPase α-subunit (Nak). Phylogeographic analysis revealed that L. cinerascens and Lineage N of L. exotica were distributed overlappingly in South Korea, Japan, and the northern region of China. Generally, the two lineages of L. exotica were distributed allopatrically, which was more evident along the coastline of mainland China than that of Japan. The results of time-calibrated phylogeny suggested that the speciation events of Ligia species might be associated with Japanese mainland formation from Oligocene to Miocene (approximately 30-5 million years ago, Mya). The results of the present study provide insights that could facilitate the understanding of the evolutionary history of Ligia, tracking of geological processes, and evolutionary effects of palaeogeographical events at the population level.

The diversity of molluscan faunas in marine lakes of Raja Ampat, West Papua, Indonesia

Abstract Marine lakes are bodies of seawater that are land-locked and maintain a subterranean connection to the surrounding sea. Here, we document the species diversity of benthic molluscs in 11 marine lakes in Raja Ampat, West Papua, Indonesia, using the roving diving survey method. We specifically tested for relationships between species richness and lake size and the degree of connection to the surrounding sea, and tested potential environmental drivers of community structure. We recorded 73 species, belonging to the classes Gastropoda (48 species, comprising 36 genera and 25 families), Bivalvia (24 species, consisting of 17 genera and 12 families), and Polyplacophora (one species). Molluscs from marine lakes are a subset of species also occurring in coral, seagrass, mangrove, and rocky shore habitats in the open sea. We found lake communities to mostly consist of grazers and filter feeders. The number of mollusc species significantly increased with increasing connection to the surrounding sea, but not with increasing surface area, indicating that dispersal potential may be the main driving force. Furthermore, we observed no significant influence of the environment on the variation in mollusc species composition among marine lakes. Still, we observed certain species to be exclusively present in either high or low-connected lakes, indicating a potential effect of environmental filtering. Marine lakes provide a unique ecosystem for diverse mollusc assemblages and as such should be protected.

Significant northern range extension of the non-native batillariid Zeacumantus subcarinatus in Australia, with observations of new habitat usage

The New Zealand batillariid gastropod Zeacumantus subcarinatus has been recorded as an invasive species in Australia since at least 1924, with populations established in rocky shore habitats at multiple locations in Greater Sydney. We observed a large population (10,000+ individuals) of Z. subcarinatus at an estuarine tidal mudflat on the New South Wales mid-north coast, representing a ~ 250 km northern range extension, and the first known record of this species in a sheltered, muddy habitat in Australia. We discuss the possible establishment means of this new population.

Gametogenic Cycle of the Oysters Pinctada capensis (Sowerby III, 1890) and Saccostrea cucullata (Born, 1778) (Class Bivalvia) in Inhaca Island, Southern Mozambique: A Subsidy for Bivalve Culture in the Region

This study describes reproductive aspects of the Pinctada capensis (pearl oyster) and Saccostrea cucullata (rocky shore oyster) in Inhaca Island, southern Mozambique (Western Indian Ocean). Adult oysters were collected monthly over two years within seagrass banks for P. capensis and rocky shore habitats for S. cucullata. The animals were evaluated using biometric and histological analyses of the gonads. Of the total population, females were predominant among larger individuals (>55 mm) and males were more dominant among smaller individuals (<55 mm) for both species. The sex ratio was (1 M–1.5 F) for Pinctada capensis and (1 M–1.6 F) for Saccostrea cucullata. Five gonad maturation stages were identified: indifferent, developing I, developing II, ripe and spent. The size at first maturity was mm and 26.2 mm for pearl oysters females and males, respectively, whereas for rocky shore oysters was 32.8 and 28.3 mm for females and males, respectively. We conclude that the reproduction of S. cucullata and P. capensis occurs mainly in summer, with a short resting period in winter, when many oysters are in the indifferent stage. These results provide valuable information to fisheries for management of both species in the area.

The ecological benefits of adding topographic complexity to seawalls vary across estuarine gradients

Artificial structures such as seawalls increasingly dominate marine urban environments. As compared to natural rocky shore habitats, seawalls are usually flat, featureless, vertical surfaces that support reduced biodiversity. One approach to increase their biodiversity is to add topographic complexity (‘complexity’) that increases microhabitat diversity and surface area. Initial investigations of the effects of complexity on the biodiversity of marine built structures found positive relationships, but more recently spatially variable effects have been found at a biogeographical scale. The present study tested whether at the scale of sites within an estuary (in this case Sydney Harbour), effects of complexity also spatially vary, and whether pollution and estuarine gradients are good predictors of this variation. Comparisons of intertidal communities colonising flat and complex (creviced / ridged) tiles, affixed to seawalls confirmed that effects of complexity were spatially variable at the site-scale, ranging from neutral to highly positive. Proximity to stormdrains, a point source of contaminants including metals, was a poor predictor of complexity effects, but effects were generally greater in the outer harbour, where biodiversity was overall greater, than the inner harbour. These results suggest that eco-engineering interventions based on complexity will not have universally positive effects and instead vary between sites separated by as little as hundreds of meters. Knowledge of spatial variation in physico-chemical conditions and the size and composition of the species pool of available colonists may assist in predicting when and where adding complexity to marine built structures will be of ecological benefit.

Elemental Chemometrics as Tools to Depict Stalked Barnacle (Pollicipes pollicipes) Harvest Locations and Food Safety.

The stalked barnacle Pollicipes pollicipes is an abundant species on the very exposed rocky shore habitats of the Spanish and Portuguese coasts, constituting also an important economical resource, as a seafood item with high commercial value. Twenty-four elements were measured by untargeted total reflection X-ray fluorescence spectroscopy (TXRF) in the edible peduncle of stalked barnacles sampled in six sites along the Portuguese western coast, comprising a total of 90 individuals. The elemental profile of 90 individuals originated from several geographical sites (N = 15 per site), were analysed using several chemometric multivariate approaches (variable in importance partial least square discriminant analysis (VIP-PLS-DA), stepwise linear discriminant analysis (S-LDA), linear discriminant analysis (LDA), random forests (RF) and canonical analysis of principal components (CAP)), to evaluate the ability of each approach to trace the geographical origin of the animals collected. As a suspension feeder, this species introduces a high degree of background noise, leading to a comparatively lower classification of the chemometric approaches based on the complete elemental profile of the peduncle (canonical analysis of principal components and linear discriminant analysis). The application of variable selection approaches such as the VIP-PLS-DA and S-LDA significantly increased the classification accuracy (77.8% and 84.4%, respectively) of the samples according to their harvesting area, while reducing the number of elements needed for this classification, and thus the background noise. Moreover, the selected elements are similar to those selected by other random and non-random approaches, reinforcing the reliability of this selection. This untargeted analytical procedure also allowed to depict the degree of risk, in terms of human consumption of these animals, highlighting the geographical areas where these delicacies presented lower values for critical elements compared to the standard thresholds for human consumption.

The invasive seaweed Asparagopsis taxiformis erodes the habitat structure and biodiversity of native algal forests in the Mediterranean Sea

Invasive seaweeds are listed among the most relevant threats to marine ecosystems worldwide. Biodiversity hotspots, such as the Mediterranean Sea, are facing multiple invasions and are expected to be severely affected by the introduction of new non-native seaweeds in the near future. In this study, we evaluated the consequences of the shift from the native Ericaria brachycarpa to the invasive Asparagopsis taxiformis habitat on the shallow rocky shores of Favignana Island (Egadi Islands, MPA, Sicily, Italy). We compared algal biomass and species composition and structure of the associated epifaunal assemblages in homogenous and mixed stands of E. brachycarpa and A. taxiformis. The results showed that the biomass of primary producers is reduced by 90% in the A. taxiformis invaded habitat compared to the E. brachycarpa native habitat. The structure of the epifaunal assemblages displayed significant variations among homogenous and mixed stands. The abundance, species richness and Shannon-Wiener diversity index of the epifaunal assemblages decreased by 89%, 78% and 40%, respectively, from homogenous stands of the native E. brachycarpa to the invasive A. taxiformis. Seaweed biomass was the structural attribute better explaining the variation in epifaunal abundance, species richness and diversity. Overall, our results suggest that the shift from E. brachycarpa to A. taxiformis habitat would drastically erode the biomass of primary producers and the associated biodiversity. We hypothesize that a complete shift from native to invasive seaweeds could ultimately lead to bottom-up effects on rocky shore habitats, with negative consequences for the ecosystem structure, functioning, and the services provided.

The surf flies of the Mascarene Islands (Diptera: Canacidae: Canacinae), with the description of a new species.

A survey of the surf flies (Canacinae) of the main Mascarene Islands of La Réunion, Mauritius, and Rodrigues was undertaken between 2015 and 2018. It encompassed intertidal material from 38 sampling stations on the islands and included records supplied by Martin J. Ebejer from La Réunion. No specimens of surf flies were collected on Rodrigues Is., which lacks suitable rocky shore habitats. A new species of Isocanace Mathis, 1982, that is deemed closely related to I. briani Mathis, 1982 described from Aldabra Is. and Madagascar, is described and figured from Mauritius (I. mauritiana Munari sp. nov.). Nocticanace mahensis (Lamb, 1912) is also reported from La Réunion and Mauritius and Procanace pninae Mathis Freidberg, 1991 from Mauritius (all representing first records for the islands concerned). The Procanace grisescens species-group is also reported from Mauritius for the first time, representing a first record of this species-group from the Mascarenes. An identification key to Afrotropical species of Isocanace is provided and the distributions of all species are mapped.

Impacts of Pervasive Climate Change and Extreme Events on Rocky Intertidal Communities: Evidence From Long-Term Data

Annual surveys of the abundance of intertidal invertebrates and macroalgae have been made at between 70 and 100 rocky intertidal time-series sites around the United Kingdom coastline since 2002 under the MarClim project. The data provide a unique opportunity to investigate the impacts of both pervasive climate change and their punctuation by extreme events on intertidal species. After the extreme storm events in the 2013/2014 winter season and the record heatwaves in the summers of 2018 and 2020, MarClim surveys recorded both physical and biological changes to rocky shore habitats. Subsequent surveys reassessed the effects on community structure via analysis of those species that resisted storm damage, those species that returned after the extreme storm events, and species that opportunistically occupied vacant habitat after storm-induced species loss. In addition, biannual storm damage surveys documenting communities recovery were carried out in the spring and winter of each year from 2014 to 2020 at three MarClim sites in north Cornwall (Crackington Haven, Trevone, and St. Ives), which experienced different types of abiotic and biotic damage resulting from these storms. Impacts of heatwaves and cold spells on the abundance of species were determined by regression on frequencies of event per year. Species of invertebrates and macroalgae generally declined in years of more frequent winter cold spells and summer heatwaves, while winter heatwaves and summer cold spells had similar numbers of positive and negative effects across species. Winter warm spells tended to have a more negative effect on cold-affinity species than on warm-affinity species. No abrupt shift was recorded after the 2013/2014 storms. Whilst a short-term change in some species was recorded in quantitative quadrat surveys, the biological communities returned to the long-term species composition and abundance within 2 years. The heatwave events caused sublethal heat damage in macroalgae, evidenced as dried areas of tissue on many individuals, with mortality-induced reductions in the abundance of only a few invertebrate species, recorded in Scotland and southwest England after the heatwave events in 2018 and 2020. MarClim and storm-damage surveys indicate that there have been no sustained impacts from either extreme thermal or storm events across the rocky intertidal communities, and biodiversity has not been significantly altered as a result. The abundance and biogeographical distributions of rocky intertidal species and communities around the United Kingdom are being driven by longer-term, large scale, pervasive change in environmental conditions, with a gradual shift towards dominance of Lusitanian species from the early 2000s in responses to warming of the marine climate.

The neritid snails of Brunei Darussalam: their geographical, ecological and conservation significance

Neritid snails are diverse and conspicuous in tropical coastal environments. They can serve as indicators of environmental change and can provide conservation information. In the present review of the neritid species of Brunei Darussalam, we report sixteen species, including seven new records from estuarine, mangrove and rocky-shore habitats. These records update distributions across the Central Indo-Pacific realm, specifically the Palawan/North Borneo ecoregion. Under-sampling, species misidentifications and locally-rare species undermine the accuracy of records in previous studies for the region. Three of the rocky-shore and two of the mangrove species collected here are represented by single specimens; these rocky-shore species are possible colonizers from neighbouring regions and the scarcity of the mangrove species likely refers to under-sampling. We present novel shell characteristics that readily distinguish between Neripteron violaceum and Nerip. cornucopia. We describe the ecology and habitat use of the Brunei species and discuss conservation issues.

Ecological niche modelling of endemic fish within La Paz Bay: Implications for conservation

Endemic marine species are useful in determining and evaluating areas for conservation. Particularly Warm Temperate Province of the Northeast Pacific (WTPNP) includes priority conservation areas, but records of endemic marine species are limited, their distributions remains generally unknown, and often excluded in extant conservation plans. Within the WTPNP, the Balandra Protected Natural Area (BPNA) is located within La Paz Bay, and it is the only management area with a developed plan. However, marine endemic fish species have not been fully considering, and their protection status requires a re-evaluation, particularly the distribution of species with adequate spatial resolution. Despite the scarce information on marine endemic fishes, ecological niche modelling allows predicting distribution areas through occurrences of the species and their relationship with a set of scenopoetic environmental variables. The abiotically suitable areas based of the endemic marine fish species within the WTPNP documented within the Bay of La Paz were modeled and the high-value areas for conservation were established through a multi-species models; these spatial patterns of suitable areas were contrasted with the current state of fish protection. Modelling was performed with the Maxent software supplied with presence-only data of 18 species and four sets of environmental layers related to the geomorphology and bottom sedimentology, as well as the Euclidean distance measures from mangrove and rocky shore habitats. We generated sixteen distribution models that revealed that only 8.4 % of the predicted area, on average, was located within a maximum state of protection within the BPNA core zone. Moreover, the generated multi-species model reveals that only 17 % of the high-value areas (≥ 9 species/hectare) were located in the core zone. These high-value areas indicate updating the current management program is required. Finally, the study illustrates how the predicted-areas can be linked to conservation strategies in the marine habitat space within and outside the BPNA.

The Northward Habitat Expansion of the Korean Top Shell Turbo sazae (Gastropoda: Vetigastropoda: Turbinidae) in the Korean Peninsula: Effects of Increasing Water Temperature

Recent global climate change often leads to poleward expansions of habitat range of marine organisms in response to increasing water temperature at high latitude. This study investigated latitudinal distribution patterns of Turbo sazae from 2009 to 2018 along the southern and eastern coasts of Korea to verify whether gradual increases in seawater temperature in the East Sea/Sea of Japan (hereafter East/Japan Sea) accelerate changes in the geographic distribution of T. sazae. Between 2009 and 2018, underwater SCUBA surveys were conducted at 19 subtidal rocky shore habitats from the southern and eastern coast of the Korean Peninsula, including Jeju Island. Additionally, long-term seawater temperature records over the last 40 years (between 1980s and 2010s) from the East/Japan Sea were analyzed to verify how changes of water temperature corresponded to geographical distributions of T. sazae. The habitat range of T. sazae was found to have extended from latitude 34°02′ N to latitude 37°06′ N from 2009 to 2018. Although seawater temperature has gradually increased since the 1990s in the East/Japan Sea, habitat expansion was particularly evident during the rapid rise of coastal seawater temperature in the 2010s. Because the strong northward expansion of the Tsushima Current can accelerate the rise of seawater temperature in the East/Japan Sea, studies of the effects of climate change on marine ecosystems of the Korean Peninsula should include data from monitoring the dynamics of the Tsushima Current.

Early to Middle Holocene Estuarine Shellfish Collecting on the Islands and Mainland Coast of the Santa Barbara Channel, California, USA

Terminal Pleistocene to Middle Holocene sea level rise resulted in a number of changes to coastal ecosystems around the world, providing new challenges and opportunities for coastal peoples. In California, glacial to interglacial sea level rise resulted in some reductions in rocky shore kelp forests, but it also resulted in the formation of estuaries. Estuaries were important for terminal Pleistocene peoples in the Santa Barbara Channel region (SBC), a pattern that persisted through the Early to Middle Holocene, and sometimes later. While paleoestuaries appear to have been relatively common along the SBC mainland coast, they were rare to absent on the Channel Islands. The Abalone Rocks Paleoestuary on Santa Rosa Island is the only well documented island estuary. However, questions remain about the size and productivity of this estuary and its importance for human subsistence and settlement relative to the more extensive mainland estuaries. Faunal data from two previously unreported site components and synthesis of shellfish data from other Abalone Rocks sites and similarly aged sites near mainland estuaries illustrate the importance of SBC mainland versus island estuaries. Estuarine shellfish were considerably more abundant at most Early and Middle Holocene mainland sites, with the Abalone Rocks Paleoestuary largely supplementary to rocky shore habitats. At island estuary sites, taxonomic richness was fairly consistent during the Early to Middle Holocene, although diversity and evenness decline slightly through time, with estuarine shellfish largely disappearing from island assemblages prior to 5000 years ago. These data demonstrate the power of archaeological research to evaluate the relationships between past environmental change and human behavior.

Effects of balancing selection and microhabitat temperature variations on heat tolerance of the intertidal black mussel Septifer virgatus

Realistic assessments of the impacts of global warming on population extinction risk are likely to require an integrated analysis of the roles of standing genetic variation, microhabitat thermal complexity, and the inter-individual variation of heat tolerance due to both genetic differences and seasonal acclimatization effects. Here, we examine whether balancing selection and microhabitat temperature heterogeneity can interact to enhance the population persistence to thermal stress for the black mussel Septifer virgatus. We deployed biomimetic data loggers on the shore to measure the microhabitat-specific thermal variation from June 2014 to April 2016. Thermal tolerance of specimens was indexed by measuring effects of temperature on heart rate. Genotyping of specimens was performed using double digestion restriction association RADSeq (ddRADseq). Our results show that inter-individual variations in thermal tolerance correlate significantly with genetic differences at some specific gene loci, and that heterozygotes have higher thermal tolerances than homozygotes. The observed seasonal changes in genotype frequency suggest that these loci are under balancing selection. The ability of thermally resistant heterozygotes to survive in sun-exposed microhabitats acts to balance the loss of homozygotes during summer and enable the persistence of genetic polymorphisms. Population persistence of the mussel is also facilitated by the micro-scale variation in temperature, which provides refugia from thermal stress. Our results emphasize that inter-individual variation in thermal tolerance and in microhabitat heterogeneity in temperature are important for the persistence of populations in rocky shore habitats.

Scale-dependent spatial and temporal patterns of abundance and population structure of the xanthid crab Leptodius exaratus on rocky shores in Kuwait

Rocky shore habitats are common in the Arabian Gulf region, however, there is little knowledge of the patterns of spatial and temporal distribution of key intertidal organisms. The spatial and seasonal distribution of Leptodius exaratus, a keystone species rocky shores on the Kuwait coast, were investigated to better understand the ecology of the species and to gain a wider knowledge of the ecology of the rocky shore habitats in the northwest Arabian Gulf. Intensive sampling across six sites along Kuwait’s coastline over a 14 month period between November 2013 and December 2014 indicated seasonal patterns of crab abundance, increasing during the warm season and decreasing during winter and with a more large crabs observed during the summer. L. exaratus was more abundant in the mid intertidal zone on both sheltered and exposed shores. Their abundance increased towards the lower shore in sheltered sites, whereas abundance was greater towards the higher shore in exposed sites. Males and females inhabit the same habitat, with similar spatial and seasonal distributions. There was high seasonal recruitment of juveniles during summer between July and September, with some low levels of recruitment later in October and November. Moreover, juveniles follow a similar pattern of distribution as adult crabs, with abundance of juveniles being directly related to the abundance of the adult crabs and ovigerous females present on each shore. This indicates direct or indirect recruitment to the adult intertidal habitat. Significant differences in crab abundance were seen between specific sampling sites, but not at a regional level between sheltered and exposed shorelines. This suggests that abundance is influenced by localised differences between rocky shore habitat, such as habitat complexity. L. exaratus is also exploited by intertidal gleaning, which may indirectly influence patterns of crab abundance through localised differences in habitat damage.

Distribution patterns of two co-existing oyster species in the northern Adriatic Sea: The native European flat oyster Ostrea edulis and the non-native Pacific oyster Magallana gigas

Abstract The Pacific oyster Magallana gigas, globally one of the most translocated marine species, has never been commercially farmed in any part of the Croatian eastern Adriatic Sea, where the native flat oyster Ostrea edulis is the only cultured oyster species. The Pacific oyster has, however, established populations on the west coast of the Istria peninsula, in the northeast Adriatic Sea. Current distribution, abundance and size structure of the flat and Pacific oysters were studied there along spatial and depth gradients in natural and artificial habitats to assess their potential for coexistence. This is the first quantitative assessment of native and non-native oyster populations on a Mediterranean-wide scale providing a baseline for determining future changes in their distribution. Both species were omnipresent, with the Pacific oyster displaying a more pronounced variability in abundance and size in relation to survey regions and depths. The population density of flat oysters was low, generally less than 1 individual/m2, with no difference among regions. The density of the Pacific oyster was significantly higher, being on average 5 individuals/m2. Dense, reef-forming aggregations of Pacific oysters were contained in Lim Bay, a nationally-important shellfish aquaculture area, where its mean density was 107 individuals/m2. Along the open coastline its densities were considerably lower and followed a latitudinal gradient. The observed abundance and size distribution patterns of the Pacific oyster suggest that Lim Bay was the introduction point, with feral settlement likely originating from short-lived experimental aquaculture trials and subsequently dispersing by prevailing local currents. The flat oysters were larger in size and settled at different depths compared to the Pacific oysters. The vertical range of Pacific oysters was mostly contained in the tidal zone, while flat oysters were present in the subtidal exclusively. Such spatial partitioning likely resulted from introduced oysters occupying a vacant ecological niche and not due to interspecific competitive exclusion. Habitat type had a strong effect on proliferation of Pacific oysters. Artificial hard substrata harboured more abundant and larger Pacific oysters than the natural rocky shore habitat. The possibility of multiple local introduction pathways in ports and marinas is also discussed. Currently, there seems to be no spatial competition between the two oyster species, but as it is hard to predict future possible impacts of non-native species, we strongly suggest regular monitoring of the Pacific oyster, prioritising sensitive and protected areas as well as areas at the edge of its distribution.

Intertidal bait gleaning on rocky shores in Kuwait

Leptodius exaratus has been used traditionally as a bait for recreational fishing in Kuwait, however little known about the effect of this practice. The present study is the first step towards understanding the recreational fisheries in Kuwait and the indirect impact of this fishery on the intertidal rocky shore habitats through bait collection, using field and questionnaire survey approaches. Anglers catch 46 different fish species, with seven of them making up more than 70% of the reported catches. L. exaratus is considered very selective for sea breams and it represented 21.4% of intertidal gleaning catches, with an average harvesting rate of 191 crabs/collector/tide. Crab gleaners reported declines in crab abundance over time and impacts on some rocky shore habitats. Direct fishing mortality was relatively low, but habitat damage caused by gleaning high, in sites with low crab abundance and fragile rock substrate, where 80% of the rocks were turned over and more than half were broken. This indicates that development of unregulated gleaning on Kuwait shores may cause irreversible ecological impacts to sensitive intertidal habitats before legislation and effective enforcement can be put in place. Partial restriction of gleaning effort and/or catches is unlikely to be effective, so that a spatial management approach identifying habitats requiring a high level of protection is needed. The latter requires information on distribution of rocky shore habitat types, refining existing intertidal habitat classifications to inform development of a sensitivity matrix approach on sufficiently fine scale to resolve variation in sensitivity of different rocky shore types to physical disturbance.

Artificial light at night erases positive interactions across trophic levels

Abstract Artificial light at night (ALAN) is one of the most recently recognized sources of anthropogenic disturbance, with potentially severe effects on biological systems that are still to be fully explored. Among marine ecosystems, high‐shore habitats are those more likely to be impacted by ALAN, due to a more intense exposition to outdoor nocturnal lightings (mostly from lamps along coastal streets and promenades, or within harbours, ports and marinas). By performing in situ nocturnal manipulations of a direct source of white LED light and presence of herbivores in a Mediterranean high‐shore habitat, we assessed the interactive effects of light pollution and grazing on two key functional components of the epilithic microbial community (the cyanobacteria, as the main photoautotrophic component, and the other bacteria, mainly dominated by heterotrophs) developing on rocky shores. Results showed an unexpected increase in the diversity of epilithic bacterial biofilm at unlit sites in the presence of grazers, that was more evident on the other (mainly heterotrophic) bacterial component, when giving weight to more abundant families. This effect was likely related to the mechanical removal of dead cells through the grazing activity of consumers. ALAN significantly modified this scenario, by reducing the density of grazers and thus erasing their effects on bacteria, and by increasing the diversity of more abundant cyanobacterial families. Overall, direct and indirect effects on ALAN resulted in a significant increase in the diversity of the photoautotrophic component and a decrease in the heterotrophic one, likely affecting key ecosystem functions acting on rocky shore habitats. ALAN may represent a threat for natural systems through the annihilation of positive interactions across trophic levels, potentially impairing the relationship between biodiversity and functioning of ecosystems and interacting with other global and local stressors currently impinging on coastal areas. A free Plain Language Summary can be found within the Supporting Information of this article.

Recruitment of the limpetPatella ulyssiponensisand its relationship with crustose coralline algae: patterns of juvenile distribution and larval settlement

AbstractRecruitment of the limpetPatella ulyssiponensiswas investigated in relation to the presence of living crustose coralline algae (CCA) in rocky-shore habitats. Juvenile limpets (≤10 mm maximum shell length) were counted in CCA-present and CCA-absent habitats, on three shores in SW Portugal during summer 2007 and winter 2009. Furthermore, the settling response of laboratory-reared larvae ofP. ulyssiponensisto CCA-covered substratum, and bare-rock, was examined. Across the intertidal zone, we found a clear association between the distribution and abundance of juveniles and the presence of CCA. Although the presence of CCA was not an absolute requisite for juvenile occurrence, null juvenile densities were mostly recorded in CCA-absent areas. The highest juvenile densities (maximum of 64 individuals in 15 × 15 cm) were consistently found in CCA-dominated habitats, namely steep wave-exposed areas at low-shore and rock-pools. The hypothesis of CCA-enhanced settlement was not supported, as settlement intensities of laboratory-reared larvae were similar between chips of rock encrusted by CCA and chips of bare-rock. From the overall number of settlers onto CCA-encrusted rock chips, 51% were found in tiny pits lacking CCA. This was the first study of the settlement patterns of larvae of the genusPatellausing naturally occurring rocky substrata. These results are preliminary and should be confirmed with choice-experiments and improved monitoring of the position of settlers. We suggest that CCA plays a role in the recruitment ofP. ulyssiponensis, potentially promoting survivorship of early benthic stages, but possibly not enhancing settlement.

Shelving the Coast With Vertipools: Retrofitting Artificial Rock Pools on Coastal Structures as Mitigation for Coastal Squeeze

Coastal squeeze caused by sea level rise threatens the size, type and quality of intertidal habitats. Along coastlines protected by hard defences, there is a risk that natural rocky shore habitat will be lost, with remaining assemblages characteristic of hard substrata confined to sea walls and breakwaters. These assemblages are likely to be less diverse and different to those found on natural shores as these structures lack features that provide moist refugia required by many organisms at low tide, such as pools and crevices. Engineering solutions can help mitigate the impacts of sea level rise by creating habitats that retain water on existing structures. However, as experimental trials are strongly affected by local conditions and motivations, the development of new techniques and solutions are important to meet the needs of local communities and developers. Following a small scale community project, a feasibility study retrofitted five concrete-cast artificial rock pools (‘Vertipools’) on to a vertical seawall on the south coast of England. After 5 years, the artificial pools increased the species richness of the sea wall and attracted mobile fauna previously absent, including fish and crabs. The Vertipools had assemblages which supported several functional groups including predators and grazers. Although disturbance of algal assemblages on the seawall from the retrofitting process was still evident after 3 years, succession to full canopy cover was underway. Collaboration between policy makers, ecologists, children and artists produced an ecologically sensitive design that delivered substantial benefits for biodiversity and that could be adapted and scaled-up to both mitigate habitat loss and enhance coastal recreational amenity.