Rocky Seashores Research Articles

Further Loss of Intertidal Mussel Stands on the Nova Scotia Coast (Canada) after the Passage of Cyclone Lee

Intertidal mussel stands are common on temperate rocky seashores and host many small invertebrates, so they are important biodiversity reservoirs. Their integrity, however, is being increasingly affected by environmental extremes. An unusual cold snap in Nova Scotia (Canada) in February 2023 was followed by the mass disappearance of mussel stands at mid-to-high elevations in rocky intertidal habitats. The present article presents evidence of significant losses at middle intertidal elevations following the passage of cyclone Lee in September 2023, which created severe surf conditions. The increasing occurrence of successive environmental extremes might challenge the persistence of these important biological systems.

Metatranscriptomics reveals diversity of symbiotic interaction and mechanisms of carbon exchange in the marine cyanolichen Lichina pygmaea.

Lichens are exemplar symbioses based upon carbon exchange between photobionts and their mycobiont hosts. Historically considered a two-way relationship, some lichen symbioses have been shown to contain multiple photobiont partners; however, the way in which these photobiont communities react to environmental change is poorly understood. Lichina pygmaea is a marine cyanolichen that inhabits rocky seashores where it is submerged in seawater during every tidal cycle. Recent work has indicated that L. pygmaea has a complex photobiont community including the cyanobionts Rivularia and Pleurocapsa. We performed rRNA-based metabarcoding and mRNA metatranscriptomics of the L. pygmaea holobiont at high and low tide to investigate community response to immersion in seawater. Carbon exchange in L. pygmaea is a dynamic process, influenced by both tidal cycle and the biology of the individual symbiotic components. The mycobiont and two cyanobiont partners exhibit distinct transcriptional responses to seawater hydration. Sugar-based compatible solutes produced by Rivularia and Pleurocapsa in response to seawater are a potential source of carbon to the mycobiont. We propose that extracellular processing of photobiont-derived polysaccharides is a fundamental step in carbon acquisition by L. pygmaea and is analogous to uptake of plant-derived carbon in ectomycorrhizal symbioses.

Ocean crossers: A tale of disjunctions and speciation in the dwarf-fruticose Lichina (lichenized Ascomycota)

Lichens thrive in rocky coastal areas in temperate and cold regions of both hemispheres. Species of the genus Lichina, which form characteristic black fruiting thalli associated with cyanobacteria, often create distinguishable bands in the intertidal and supralittoral zones. The present study uses a comprehensive specimen dataset and four gene loci to (1) delineate and discuss species boundaries in this genus, (2) assess evolutionary relationships among species, and (3) infer the most likely causes of their current geographic distribution in the Northern and Southern hemispheres. A dated phylogeny describes the time frame in which extant disjunctions of species and populations were established. The results showed that the genus is integrated by four species, with Lichina pygmaea, L. confinis and the newly described L. canariensis from rocky seashores in the Canary Islands, occurring in the Northern Hemisphere, whereas L. intermedia is restricted to the Southern Hemisphere. Lichina intermedia hosted a much higher intraspecific genetic diversity than the other species, with subclades interpreted as species-level lineages by the different species delimitation approaches. However, a conservative taxonomic approach was adopted. This species showed a striking disjunct distribution between Australasia and southern South America. The timing for the observed interspecific and intraspecific divergences and population disjunctions postdated continental plate movements, suggesting that long-distance dispersal across body waters in the two hemispheres played a major role in shaping the current species distributions. Such ocean crossings were, as in L. canariensis, followed by speciation. New substitution rates for the nrITS of the genus Lichina were inferred using a tree spanning the major Ascomycota lineages calibrated using fossils. In conclusion, this work lays the foundation for a better understanding of the evolution through time and space of maritime lichens.

Lichen zonation on UK rocky seashores: a trait-based approach to delineating marine and maritime lichens

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Where three snail species attach while emersed in relation to heterogenous substrate temperatures underneath intertidal boulders.

Mobile intertidal gastropods can employ behavioural thermoregulation to mitigate thermal stress, which may include retreating under boulders when emersed. However, little is known about how gastropod occupancy of under-boulder habitats is associated with any variations in substrate temperature that exist under boulders. Thermal imagery was used to measure the temperature of boulder lower surfaces and investigate how three snail species were associated at low tide with the maximum and average temperatures underneath grey siltstone and quartzite. Lower boulder surfaces had heterogeneous temperatures, with grey siltstone having temperature gradients and quartzite temperature showing mosaics. Temperature differences between the hottest and coolest gradient or mosaic locations were >5 °C; thus there was a range of temperatures that snails could interact with. All three snail species occupied cooler parts of temperature mosaics or gradients, avoiding the hottest areas. Stronger associations were detected on the hotter grey siltstone and for the more-thermally sensitive Nerita atramentosa and Diloma concameratum. Even though snails were associated with cooler areas, some individuals were still exposed to extreme substratum heat (>50 °C). These results suggest that gastropod thermoregulatory behaviour is far more complex than simply retreating underneath boulders at low tide, as there is also a range of under-boulder temperatures that they interact with. Untangling interactions between intertidal gastropods and heterogenous substrate temperatures is important given rocky seashores already represent a thermally-variable and potentially-stressful habitat, which may be exacerbated further given predictions of warming temperatures associated with climate change.

How extraordinary periwinkles withstand extreme heat

Life might seem to be less eventful for the tenacious creatures that carve out life on the rocky sea shore once the tide has receded, but looks can be deceptive. Once the tide has gone on sunny days, many molluscs are at risk of baking in the sun. But the heat holds little fear for one species making a living on the southern coast of China. ‘The rocky intertidal periwinkle snail, Echinolittorina malaccana, is one of the most heat-tolerant eukaryotes, with an upper lethal thermal temperature of near 55°C’, says Yun-wei Dong from Ocean University of China. So how do these masters of heat deal with wilting conditions? And how hot does it really get once the surf has departed? Intrigued by the molluscs’ robust reputation, Dong, with colleagues from Xiamen University and his own institution, set about finding out just how hot knobbly periwinkles get.First, Dong secured iButton thermometers alongside the hardy snails on their rocky shore homes for 3 months during the summer in 2014 and was impressed to see that the average temperature generally hovered around 32°C. However, the temperatures did creep much higher from time to time and on one occasion even exceeded an extraordinary 53°C. But what effect did these high temperatures have on the mini molluscs’ beating hearts?Warming the snails gradually from 25°C, the team discovered that the heat really set the periwinkles’ hearts racing, first hitting a high of ∼42 beats min–1 at 37°C before reaching a second high of ∼67 beats min–1 at 52°C, after which they began to fail. ‘According to the heart beat curve of E. malaccana, metabolic depression occurs from ∼37°C to ∼45°C’, says Dong, adding that this reduction in metabolic rate helps the snails to survive at intermediate temperatures.The team then tracked the compounds produced in the snails’ bodies as a result of their metabolism and realised that the hardy molluscs switched from running their metabolism on oxygen at lower temperatures to other sources of energy for anaerobic metabolism at temperatures above 37°C. ‘High temperature and hypoxia [low oxygen] commonly co-occur in intertidal molluscs’, says Dong, explaining that shifting to anaerobic metabolism as the temperature soars allows the periwinkles to continue meeting their energy demands as their oxygen supplies dwindle.In addition, the team noticed that the snails produced a range of compounds that protect them from the damaging effects of heat. They included molecules that mop up toxic forms of oxygen – reactive oxygen species – that are produced by stress, modified lipids that improve the stability of cell membranes at high temperatures and a range of small molecules that protect proteins from damage. ‘This thermophilic species thus can deploy a wide array of adaptive strategies to acclimatise to extremely high temperatures’, says Dong.Having revealed the physiological mechanisms that underpin the periwinkle's extraordinary resilience, Dong and his team are eager to discover how the modest mollusc's genome has adapted to permit them to endure such extreme temperatures. But he is also concerned about their future. ‘We will apply these physiological traits in a species distribution model for predicating species biogeographic pattern in the context of climate change’, says Dong.

Rocks of different mineralogy show different temperature characteristics: implications for biodiversity on rocky seashores.

As some intertidal biota presently live near their upper tolerable thermal limits when emersed, predicted hotter temperatures and an increased frequency of extreme-heat events associated with global climate change may challenge the survival and persistence of such species. To predict the biological ramifications of climate change on rocky seashores, ecologists have collected baseline rock temperature data, which has shown substrate temperature is heterogenous in the rocky intertidal zone. A multitude of factors may affect rock temperature, although the potential roles of boulder surface (upper versus lower), lithology (rock type) and minerology have been largely neglected to date. Consequently, a common-garden experiment using intertidal boulders of six rock types tested whether temperature characteristics differed among rock types, boulder surfaces, and whether temperature characteristics were associated with rock mineralogy. The temperature of the upper and lower surfaces of all six rock types was heterogeneous at the millimetre to centimetre scale. Three qualitative patterns of temperature difference were identified on boulder surfaces: gradients; mosaics; and limited heterogeneity. The frequency of occurrence of these temperature patterns was heavily influenced by cloud cover. Upper surfaces were generally hotter than lower surfaces, plus purple siltstone and grey siltstone consistently had the hottest temperatures and white limestone and quartzite the coolest. Each rock type had unique mineralogy, with maximum temperatures correlated with the highest metallic oxide and trace metal content of rocks. These baseline data show that rock type, boulder surface and mineralogy all contribute to patterns of heterogenous substrate temperature, with the geological history of rocky seashores potentially influencing the future fate of species and populations under various climate change scenarios.

To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus

Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix. Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n). The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans). The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles. We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France. Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase. Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes. The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae. Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.

Thermal and salinity effects on locomotor performance of Thoropa taophora tadpoles (Anura, Cycloramphidae).

It is well known that environmental temperature influences several biological functions of ectotherms, notably in amphibians. The high permeability of anuran skin, associated with the effect of elevated environmental temperature, potentiates the dehydration process and this combination may restrict locomotor performance. Thoropa taophora is an endemic species from the Atlantic Rainforest whose tadpoles are semiterrestrial and predominantly diurnal, and are found in rocky seashores where they are exposed to sea spray and high temperatures. In this study we investigated how temperature and salinity conditions affect the locomotor performance in Thoropa taophora tadpoles. We also assessed how different osmotic concentrations affect the activity of the metabolic pathways that support muscle function. We measured the sprint speed of tadpoles of various sizes at different temperatures and salinities in the field. We also measured the activity of the enzymes pyruvate kinase (PK), lactate dehydrogenase (LDH) and citrate synthase (CS) in different temperatures and osmotic concentrations, and calculated the thermal sensitivity and the activity constants for each osmolality. Our results showed that, in general, sprint speed decreased with increasing temperature and salinity. However, whereas the effect of increased salinity was similar in smaller and larger tadpoles, increased temperature had a higher negative impact on sprint speed of larger tadpoles, thus indicating low thermal sensitivity of small tadpoles. PK and LDH thermal sensitivities and LDH constant of activity decreased as the osmolality increased. In conclusion, the locomotor capacity of tadpoles was decreased by temperature and salinity, which may be related to a decrease in anaerobic metabolism both in terms of sensitivity and total energy turnover through enzymatic activity. We discuss the ecological consequences, including the potential impacts on predator escape behavior promoted by changes in metabolism and locomotor performance in an early stage of development of this species.

Differential responses to salt concentrations of lichen photobiont strains isolated from lichens occurring in different littoral zones

AbstractAn interesting biota of lichen-forming fungi occurs along rocky seashores of cold and warm-temperate regions in both hemispheres. Most of the species belong to the family Verrucariaceae and form symbioses with an extraordinarily diverse group of photobionts. We isolated the photobionts of three species: Hydropunctaria maura and H. amphibia from the supralittoral zone, and Wahlenbergiella striatula from the upper intertidal zone. We characterized the isolated strains structurally by means of transmission electron microscopy, and molecularly using the nrSSU and nrITS and chloroplast RPL10A regions. Additionally, we studied the response of the strains to different salt concentrations, analyzed the concentration of osmoregulatory solutes, and measured photosynthesis performance by chlorophyll fluorescence and CO2 assimilation techniques. All strains belong to the recently described species Halofilum ramosum, although we found differences in the ITS and RPL10A regions among the strains shared by H. maura and H. amphibia and the strain isolated from W. striatula. Differences were also found in the main osmoregulatory response of the strains growing under high salt concentrations: W. striatula accumulated glycerol, while H. maura and H. amphibia synthetized sucrose. Analyses of photosynthesis performance also indicated differences in physiological behavior between supralittoral-dwelling and intertidal-dwelling species, W. striatula showing lower photosynthetic activity under high irradiance. Our results highlight the role of photobionts in determining lichen zonation on rocky seashores.

Tom Beeckman

Tom Beeckman

Empidoid flies from Cabo Verde (Diptera, Empidoidea, Dolichopodidae and Hybotidae) are not only composed of Old World tropical species

In the present paper three empidoid fly species new to science are described from the island Sal of the Cabo Verde archipelago. Aphrosylus salensis sp. nov., a marine dolichopodid fly, is found in the splash zone of a rocky sea shore. Crossopalpus salensis sp. nov. (Hybotidae) is recorded from two different sebkhas. Crossopalpus complicatus sp. nov. is reported from an irrigated wasteland. A key to the species of these two genera occurring on Cape Verde is provided. The presence of the morphospecies Tachytrechus tessellatus (Macquart, 1842), a presumed Old World tropical species, is confirmed.

A revision of the genus Littorina (Mollusca: Gastropoda) in Korea

ABSTRACTLittorina Férussac, 1822 is an abundant genus of small gastropods found in the upper littoral zone of rocky seashores worldwide. Although ecologically important, shell-based species identification in this genus is challenging due to phenotypic variation in shell morphology and lack of diagnostic characters among morphologically similar species. In this study, we revised the taxonomy of Korean Littorina species using morphological characters (shell and radula) and cox1 mitochondrial DNA sequences for three Korean species: L. brevicula, L. sitkana, and L. horikawai. Results suggest that L. sitkana was erroneously reported as L. kasatka in a previous study. A new record for Littorina horikawai (Matsubayashi & Habe in Habe, 1979), previously unknown from Korea, is described, which can be distinguished from L. sitkana by the presence of alternating white and brown spiral ribs on each whorl. Comparison of the mtDNA cox1 gene sequences shows very low intraspecific variation even between geographically distant populations. A phylogenetic tree supports a close relationship between L. horikawai and L. sitkana, consistent with earlier phylogenetic studies.

The instability of field experiments: building an experimental research tradition on the rocky seashores (1950-1985).

In many experimental sciences, like particle physics or molecular biology, the proper place for establishing facts is the laboratory. In the sciences of population biology, however, the laboratory is often seen as a poor approximation of what occurs in nature. Results obtained in the field are usually more convincing. This raises special problems: it is much more difficult to obtain stable, repeatable results in the field, where environmental conditions vary out of the experimenter's control, than in the laboratory. We examine here how this problem affected an influential experimental research tradition in community ecology, the study of the ecology of the rocky seashores. In the 1960s, a handful of North-American ecologists, most notably Joseph Connell, Robert Paine and Paul Dayton, made the rocky seashores a model study system for experimenting in the field. Their experiments were deceptively simple: they removed species living on the seashore and described the resulting effects on the local ecology. These experiments exerted a deep influence on community ecology. They provided evidence for speculative developments concerning the theory of interspecific competition, the factors responsible for species richness and the ecology of food webs. They also stimulated novel conceptual developments. In particular, Paine developed the predation hypothesis, which states that the presence of predators can favour species richness, before introducing the keystone species concept, according to which some species exert disproportionate effects on ecological systems. More broadly, these experiments gave support to a methodological trend in favour of field experimentation. Only controlled perturbations in the field, it seemed, provided a reliable method to get insights into the structure of ecological communities. However, as experiments were continued in time and repeated in different sites, divergent results appeared. We analyse here how intertidal researchers coped with the variability of environmental conditions and tried to stabilize their results. In the process, they reconsidered not only their early conclusions, but also the exclusive status given to field experiments. Expanding on this case study, we discuss some significant differences between laboratory and field experiments.

Metal-Mediated Molecular Self-Healing in Histidine-Rich Mussel Peptides

Mussels withstand high-energy wave impacts in rocky seashore habitats by fastening tightly to surfaces with tough and self-healing proteinaceous fibers called byssal threads. Thread mechanical behavior is believed to arise from reversibly breakable metal coordination cross-links embedded in histidine-rich protein domains (HRDs) in the principle load-bearing proteins comprising the fibrous thread core. In order to investigate HRD behavior at the molecular level, we have synthesized a histidine-rich peptide derived from mussel proteins (His5-bys) and studied its reversible adhesive self-interaction in the presence and absence of metal ions using PEG-based soft-colloidal probes (SCPs). Adhesion energies of greater than 0.3 mJ/m(2) were measured in the presence of metal ions, and the stiffness of the modified SCPs exhibited a 3-fold increase, whereas no adhesion was observed in the absence of metals. Raman spectroscopy confirmed the presence of metal-coordination via histidine residues by the peptide-supporting the role of His-metal complexes in the mechanical behavior of the byssus.

Interplay of experimental harvesting and climate-related disturbance on benthic assemblages of rocky seashores

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 495:131-142 (2014) - DOI: https://doi.org/10.3354/meps10574 Interplay of experimental harvesting and climate-related disturbance on benthic assemblages of rocky seashores J. P. Oliveira1,2,*, I. Sousa-Pinto2,3, G. M. Weber1,2,4, I. Bertocci2 1Estação Litoral da Aguda, Rua Alfredo Dias, praia da Aguda, 4410-475 Arcozelo/VNG, Portugal 2CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal 3Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal 4ICBAS - Institute of Biomedical Sciences Abel Salazar, Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal *Corresponding author: zpoliveira@gmail.com ABSTRACT: Assessing responses of assemblages to compounded perturbations is a crucial goal of research on ecological impacts of current and predicted environmental changes. We examined the interactive effects of changes in temporal variance of ‘storm-like’ mechanical disturbance and in harvesting intensity of mussels (Mytilus galloprovincialis) on sessile assemblages associated with mussel beds on rocky shores in north Portugal. Response variables were measures of temporal variance in the abundance of individual taxa and in the structure of assemblages. Events of disturbance regularly distributed over the period of the experiment determined a lower temporal heterogeneity in assemblage structure than events characterized by larger temporal variance, but only in the presence of an intact mussel bed. The opposite pattern was documented in plots where mussels were completely removed. Univariate results indicated taxon-specific responses, with macroalgae, such as Corallina spp. and Porphyra linearis, showing patterns analogous to the multivariate one and grazing invertebrates displaying an opposite trend. This is one of the first manipulative examples aimed at examining effects of temporal variability of climate-related disturbance on systems increasingly depleted by concomitant stressors. The main finding was that effects of temporal variability of climate-related events of mechanical disturbance are mediated by the presence of intact stands of a foundation species. This may contribute to understanding and predicting responses of populations and assemblages under scenarios of concomitant climatic and nonclimatic changes. KEY WORDS: Climate change · Foundation species · Multiple disturbances · Mytilus galloprovincialis · Human impact · Intertidal · Temporal variance Full text in pdf format Supplementary material PreviousNextCite this article as: Oliveira JP, Sousa-Pinto I, Weber GM, Bertocci I (2014) Interplay of experimental harvesting and climate-related disturbance on benthic assemblages of rocky seashores. Mar Ecol Prog Ser 495:131-142. https://doi.org/10.3354/meps10574 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 495. Online publication date: January 09, 2014 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2014 Inter-Research.

BIODIVERSITY OF THE BUNDALA NATIONAL PARK AND RAMSAR WETLAND

The Bundala National Park (6216 ha) is located in the Hambantota District, within the SoutheasternArid Zone of Sri Lanka. T:lC park consists mainly of dry thorny scrubland and lagoons;Koholankala (390 ha), Malala (6511 ha), Embilikala (430 ha) and Bundala (520 ha). Theseshallow water lagoons form a complex wetland system that harbours a rich bird life, includingseveral species of migratory waterfowl. Bundala is Sri Lanka's first Ramsar wetland-a wetlandof international importance especially for migratory waterfowl. Recent studies have indicatedthat the Bundala National Park and its wetlands are being degraded by various adverse factors,Therefore, this survey was intended to document the present status of biodiversity in thisprotected area, and the threats it faces today. The systematic survey on biodiversity extendedfrom January to April 2001, during which the area was visited at fortnightly intervals, The faunaand flora were surveyed using scientifically valid inventorying techniques. The survey enabledto document 7 major terrestrial vege.ation/habitat types (dry thorny scrubland, Arid zone forests,sand dune vegetation, gentle sea shore vegetation, Arid zone maritime grasslands/pastures,Riverine forest, anomalous Mesquite (Prosopis) scrublands and 6 wetland types (saltmarsh,mangrove, brackish water lagoons, sandy and rocky sea shore, seasonal water holes and tanks,saltern) in Bundala. A total of j83 plant species have been documented from the abovevegetation and habitat types in Bundala, These include 6 endemics and 7 species that areconsidered as nationally threatened. Shrubs and herbs (plants with leaves and non-woody stems)are the predominant plant life forms of Bundala. A total of 324 species of vertebrates have beenrecorded from Bundala National Park, of which 11 species are endemic, while 29 species arenationally threatened. Among the invertebrates are 52 species of colourful butterflies.At present, the biodiversity of Bundala National Park is facing several threats, which could besummarised under habitat deterioration and degradation (i.e., Shell mining, driving of vehiclesoff the recommended tracks, discharge of irrigation water in to lagoons, release of sludge intothe Bundala lagoon), direct exploitation of species (poaching, felling of trees, road kills), spreadof invasive alien species (4 animal species and 15 plant species), prolonged drought, andunplanned land-use practices. It is envisaged that these findings would contribute to theconservation and management of this globally important PA

Habitat selection by anurofauna community at rocky seashore in coastal Atlantic Forest, Southeastern Brazil

Rocky seashores are low granitic hills distributed along the southeastern Brazilian coast with xeric-like vegetation due to the shallow soil. Knowledge on amphibian communities and their reproductive patterns is especially reduced on this kind of environment. Herein, we present a framework of two years monitoring an amphibian community at a rocky seashore environment located at the protected area of Parque Estadual da Serra da Tiririca, municipality of Niterói, state of Rio de Janeiro, Brazil. We conducted diurnal and nocturnal searches for frogs in tank bromeliads, rocky surface and shrubby vegetation. Annual pattern of breeding activity of anurans was also estimated. Individuals of the most abundant tank-bromeliad, Alcantarea glaziouana were collected and measured according to several variables to understanding the selection of bromeliads by frogs. We checked the influence of the environmental conditions on amphibian abundance, association between the bromeliads measures, and the water storage in the tank. We recorded the species: Scinax aff. x-signatus; S. cuspidatus; S. littoreus; Thoropa miliaris and Gastrotheca sp. Bromeliads were the preferential habitat used by anurans. The nocturnal habit was predominant for all species and during diurnal searches, the specimens were found sheltered in bromeliads axils. The number of calling males as well as amphibian abundance was associated with the rainiest and warmest period of the year. The species S. littoreus was observed in breeding activity in the majority of sample period. Adult calling males of T. miliaris were observed especially in the rainy season. Rainfall and temperature combined are positively correlated to the total number of captured amphibians. However, individually, rainfall was not significantly correlated, while temperature was positively correlated with the amphibian abundance. Water storage capacity by bromeliads was correlated to characteristics and size of the plant. In the rainy season, the height of the plant and the diameter on top view were correlated with the occurrence of amphibians, while during the driest period there was no correlation among variables and the bromeliad usage by amphibians. Recorded species were strongly associated to the Atlantic Forest domain. Nevertheless, the occupation of rocky seashores by anurans may be more associated with the specialized reproductive modes presented by species, since there is no permanent water available in ponds or streams.

Endocarpon maritima sp. nov. (lichenized Ascomycota) from the maritime region of South Korea

A new lichenized fungus, Endocarpon maritima, was collected from the rocky seashores of Bogil Island, South Korea. The species is characterized by a squamulose to lobate brown to olive-green thallus attached to the substratum by rhizohyphae, blackish hypothallus, 2-spored asci, spores 25–37.5(–50) × (10.5–)13–17(–20) μm, and a maritime distribution. Lichenized species of Aspicilia, Buellia, Caloplaca, Phaeophyscia, Physcia, and Pterygiopsis occur in close association with E. maritima.

Phylogenetic position of the brown algae-associated lichenized fungus Verrucaria tavaresiae (Verrucariaceae)

Abstract The lichen species Verrucaria tavaresiae is associated with Petroderma maculiforme, an unusual photobiont belonging to the brown algae. This marine ascomycete has so far been found only on the coast of Central California, although its photobiont has a much broader geographical distribution. Morphological and ecological characters have led a previous author to attribute this marine fungus to Verrucaria, a genus including other species colonizing the intertidal zone of rocky seashores. Phylogenetic inferences from two nuclear and one mitochondrial loci confirm the placement of Verrucaria tavaresiae within the lichen family Verrucariaceae and more specifically within the marine genus Wahlenbergiella. The new combination Wahlenbergiella tavaresiae (R.L. Moe) Gueidan, Thus & Perez-Ortega is therefore proposed here.