The global adoption of e-scooters as a convenient mode of micro-mobility transportation is on the rise, offering a flexible solution for covering first- and last-mile journeys. However, this surge in usage brings challenges, particularly concerning road safety, as e-scooter riders often share road space with other vehicles, heightening the risk of serious accidents. While numerous studies have explored safe overtaking behaviors and safety perceptions from drivers’ viewpoints, limited attention has been given to understanding the varying safety perceptions of both drivers and e-scooter riders, particularly after riding an e-scooter and being overtaken by their own vehicles. This research aims to bridge this gap by examining variations in safety perceptions and assessing behavioral changes before and after experiencing overtaking scenarios. Specifically, the study focuses on scenarios where an e-scooter rider experiences being overtaken by a vehicle they had previously driven. A Unity-based sequential simulation process is employed to replay scenarios obtained from a vehicle simulator during an e-scooter experiment involving the same participant without their awareness. This innovative approach allows e-scooter rider participants to relive their own prior vehicle overtaking maneuvers while riding an e-scooter. The findings reveal that most participants (64%) felt less safe as e-scooter riders, influenced by factors like relative speed and acceleration of overtaking vehicles. After experiencing being overtaken by their own pre-driven vehicles, a noteworthy positive correlation emerged between safety perception and lateral distance, indicating that greater distance is derived from a better understanding of e-scooter safety. The study demonstrates the effectiveness of the sequential simulation strategy in fostering safe driving behavior and raising road safety awareness. Experiencing overtaking behaviors firsthand as an e-scooter rider, previously behind the wheel of the overtaking vehicle, encourages a heightened awareness of road safety. These findings have significant implications for road safety authorities, suggesting the potential application of this approach in driver education programs. By incorporating such interventions tailored to improve the safety of vulnerable road users, authorities can take proactive steps towards mitigating risks associated with micro-mobility transportation.