This study analyzes and evaluates the relationship of surface and groundwater of the Syrdarya River within Kazakhstan. The share of groundwater recharge of the river was calculated by two methods: by genetically partitioning the total runoff hydrograph and by calculating low f low. The difference of groundwater flow into the river between the selected hydrostations is also calculated. A comparison of the obtained results for the quasi-natural and disturbed periods is made. In natural conditions, the infiltration of river runoff into underground horizons prevailed along the length of the Syrdarya River, however, under the influence of anthropogenic activity, the picture changed to the opposite: the discharge of groundwater prevails along the length of the Syrdarya River, including the emergence of filtration waters below the dam of the Shardara HPP, collector-drainage and discharge waters from irrigation f ields of the adjacent territory. Also, apparently, the truncation of spring flood peaks leads to an increase in the groundwater level and an increase in the share of underground river supply. Recommendations for the application of the methods used in the work are given.