The effects of coconut shell biochar and coke on anaerobic digestion of river snail rice noodle wastewater treatment were assessed, and the microbial community, and methane metabolic pathways were investigated. When the hydraulic retention time was 24 h, the average chemical oxygen demand (COD) removal rates in the reactors with coconut shell biochar and coke were 94.02% and 88.68%, respectively; when the hydraulic retention time was 12 h, the average COD removal rates were 91.32% and 85.47%, respectively. The addition of coconut shell biochar and coke increased the activity of protease in the sludge from 0.45% to 29.31% and from 1.00% to 21.35%, respectively. The addition of coconut shell biochar and coke to the two anaerobic reactors promoted the growth of Euryarchaeota, Proteobacteria, and Chloroflexi. In glycolysis, the key genes glk, pfk and pk were upregulated by 3.15%, 5.22%, and 0.44% in the coconut shell biochar reactor and 8.97%, 1.93% and 3.73% in the coke reactor, respectively, and the keytricarboxylic acid (TCA) cycle genes kor, frd, and mdh were also up-regulated.
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