River Estuary Region Research Articles

Sediment oxygen uptake and hypoxia in coastal oceans, the Pearl River Estuary region

Hypoxia is increasing in coastal oceans due to high oxygen consumption and weak ventilation. Quantifying biological oxygen uptake and how its effects on hypoxia respond to stratification is important for management and predicting future trends. This work introduces a simple analysis to quantify and compare the biological and physical drivers of hypoxia, by using an example from the Pearl River Estuary (PRE) region (10–70 m deep). We show that in the PRE region, sediment respires ∼50 % of organic matter produced in the water column and oxidizes ∼88 % of the ammonium produced from sediment organic matter degradation. These processes lead to high sediment oxygen uptake (SOU; 41.1 ± 16.3 mmol m-2d-1). Under stratification, sediment's effect on the bottom oxygen is strongly regulated by the thickness of the bottom boundary layer (BBL), and the robust relationships can be used to parameterize SOU. We then construct a simple and generic mass-balance model to estimate the water column oxygen uptake in the BBL (WOUBBL) and quantify the total oxygen loss. By comparing model results to observations in the PRE and other similar systems, we show that the sensitivity of oxygen levels to SOU is largely controlled by the duration of stratification, while the organic matter settling velocity controls the contributions of SOU to total oxygen consumption. We further demonstrate how the model can help evaluate the primary drivers of hypoxia (stratification versus high oxygen consumption), determine the time scale of stratification required to develop hypoxia, and explain the within and across system variabilities.

High O3 pollution initiated by cold front passage over Pearl River Estuary

The influence of cold fronts on the formation of ozone (O3) pollution, particularly the contributions of physical and chemical processes to O3 abundance during the passage of cold fronts in winter over the Pearl River Estuary (PRE) region was still unclear. This study provided an in-depth analysis on the occurrence of high O3 pollution based on data from field measurements and simulation of a chemical transport model (i.e., WRF-Chem) over the PRE region during winter 2021 when cold fronts were frequently observed. Totally 7 high O3 episode days were observed during pre- and post-frontal stages at DWS (i.e., Da Wan Shan island), a downwind island site of the inland Pearl River Delta (PRD) region, which were accompanied with the prevailing northerly wind patterns with insignificant variations of wind speeds in a stable boundary layer over the whole PRE region. The quantitative analysis based on simulation results indicated that horizontal transport and chemical processes accounted for the buildup of O3 abundance at DWS, with the highest mean rates of 7.3 and 5.6 ppbv/h at pre- and post-frontal stages, respectively. The high contributions from horizontal transport to O3 levels at DWS were associated with the persistent northerly winds with small variations, while the contributions of chemical processes were varied with the abundance of precursors and meteorological conditions. However, vertical transport made negative contributions to O3 abundance, with the most significant mean diffusion rate of −2.6 ppbv/h at the frontal stage because of the control of high-pressure system, while the less influence of vertical transport at pre- and post-frontal stages was associated with the weak pressure gradient forces and wind patterns. The sensitivity analysis further demonstrated that the emissions of precursors from the inland PRD region contributed 50–76% to O3 abundance over PRE at different stages. Overall, the above results quantitatively confirmed the dominant role of transport from inland PRD to the O3 pollution over the downwind PRE region and were helpful for better understanding of O3 pollution in winter over PRE.

Analyzing the spatio-temporal correlation between tide and shipping behavior at estuarine port for energy-saving purposes

Estuarine ports account for more than 80% of world ports. Considering the large portion of ships depart and arrive at estuarine ports worldwide, using current and tide to departure and arrive at the estuarine ports can help to save energy and reduce fuel consumption and pollution emissions. To better understand to what extent ships take and against the tide when entering and leaving estuarine port for energy-saving purposes, this study collected AIS data of ships in the Pearl River Estuary region in 2022 and fused it with the simulated current data to unveil the ship behavior patterns. It is found that most of the ships sailed against tide among the 225,333 ships entered and left the harbor in 2022. 75–76% of the ships choose to enter the harbor against current, 9% choose to enter the harbor in transverse current, and only 15–16% choose to take tide. In terms of outbound ships, the proportions of ships sailed against tide equals to the proportion take tide, each took 41–45%, 11–15% sailed in the transverse current direction. Our result revealed that the outbound ships is better at take tide than inbound ships, especially at the tide with a speed higher than 0.6 m/s. The methods demonstrated in this study can be adapted and applied to other estuarine ports to understand the impact of the tide on shipping energy consumption. Future studies should focus on increasing the energy efficiency of ships by better planning the shipping schedule and taking advantage of the tide while leaving and entering the ports.

Navigating University Openness in Research Policy Inconsistent with Indigenous Data Sovereignty: A Case Analysis.

Indigenous nations and communities in the United States have rights as sovereign governments to exercise control and ownership over all data and information generated by or from the tribes, tribal members, or tribal resources. Indigenous nations exercise these rights through data ownership policies established in response to unethical research practices in research involving Indigenous communities. Most universities in the U.S. have "openness in research" policies to ensure academic freedom to publish freely, exercised by retaining university control of data. Here, we describe our study of cultural ecosystem services in the St. Louis River estuary region (Nagaajiwanaang in the language Ojibwemowin) in Duluth, Minnesota, and Superior, Wisconsin, U.S., an area that includes portions of the 1854 and 1842 Ceded Territories and reservation lands of a local band of Ojibwe (hereafter referred to as "the Band"). In this university-led, Band-supported study, both the university and the Band sought ownership of data collected based on their respective policies, resulting in a research delay of nearly a year. We found that open research policies that do not consider Indigenous sovereignty can hamper collaboration between university researchers and tribal nations, even when there is broad agreement on research goals and objectives. University open research policies that do not explicitly address Indigenous sovereignty fall short of the open research principles they intend to support and should be revised. Formal adoption of principles for ethical research with sovereign tribal governments by universities is needed to improve coordination and trust among university and tribal researchers and members.

Distribution Characteristics of Microplastics in Surface Seawater off the Yangtze River Estuary Section and Analysis of Ecological Risk Assessment.

Microplastics are widespread in the oceans as a new type of pollutant. Due to the special geographical environment characteristics, the Yangtze River estuary region become hotspot for microplastics research. In 2017 and 2019, surface seawater microplastics samples were collected from five stations off the Yangtze River estuary during four seasons (spring, summer, autumn, and winter). The abundance and characteristics of microplastics in seawater were researched. The results showed that microplastics widely existed in surface seawater; the average abundance of microplastics in seawater was (0.17 ± 0.14) items/m3 (0.00561 ± 0.00462) mg/m3; and accounting for 80% of the total plastic debris, the abundance of microplastics was at moderately low levels compared to national and international studies. The particle size of most microplastics was between 1 mm to 2 mm, accounting for 36.1% of the total microplastics. The main shapes of microplastics were fiber, flake, and line, accounting for 39.5%, 28.4%, and 20.8%, respectively. Polypropylene, polyethylene terephthalate, and polyethylene were the main components of microplastics, accounting for 41.0%, 25.1%, and 24.9%, respectively. Yellow, green, black, and transparent were the most common colors, accounting for 21.9%, 19.6%, 16.5%, and 15.7%, respectively. This study shows that the spatial distribution of microplastics in the surface waters off the Yangtze River estuary shows a decreasing trend from nearshore to farshore due to the influence of land-based inputs, hydrodynamics, and human activities; the distribution of microplastics has obvious seasonal changes, and the level of microplastic pollution is higher in summer. The potential ecological risk of microplastics in the surface waters off the Yangtze River estuary is relatively small.

Terrestrial nanoparticle contaminants and geospatial optics using the Sentinel-3B OLCI satellite in the Tinto River estuary region of the Iberian Peninsula

The Tinto River is known globally for having a reddish color due to the high concentration of dissolved metals in its waters. The general objective of this study is to analyze the dispersion of nanoparticles (NPs) and ultra-fine particles in terrestrial and geospatial suspended sediments (SSs) using Sentinel-3B OLCI (Ocean Land Color Instrument) satellite images; by examining water turbidity levels (TSM_NN), suspended pollution potential (ADG_443_NN) and presence of chlorophyll-a (CHL_NN). The images were collected in the estuary of the Tinto River, in the city of Nerva, Spanish province of Huelva, between 2019 and 2021. The following hazardous elements were identified in nanoparticles and ultra-fine particles by FE-SEM/EDS: As, Cd, Ni, V, Se, Mo, Pb, Sb and Sn. Sentinel-3B OLCI satellite images detected a 2019 TSM_NN of 23.47 g−3, and a 2021 reading of 16.38 g−3.

Spatial variation, sources, and potential ecological risk of metals in sediment in the northern South China Sea

Heavy metals are of great concern to humans because of their persistence, bioaccumulation, and toxicity. A study on the spatial variation, sources, and potential ecological risk of heavy metals in the coastal sedimentary environment are helpful to clarify the pollution history of aquatic systems and effectively manage and control heavy metal pollution. However, most studies are limited to the Pearl River Estuary region. To investigate the spatial distribution characteristics, sources, and potential ecological risks of heavy metals (Cr, Cu, Pb, Ni, Sn, Zn, As, Cd), a total of 41 sediment samples from the northern South China Sea (NSCS) region were analyzed. The results show that Cu, Ni, Pb, and Zn have similar distribution patterns and their migration path in the coastal area is mainly controlled by the coastal current in western Guangdong. Meanwhile, these metals also have similar sources, i.e. natural weathering products. The distribution patterns of Cr, Sn, Cd, and As are not affected by the coastal current, and the sources of these metals are not only natural weathering products but also human inputs such as electroplating and electronic industries and runoff from agricultural sites. Agricultural activities, coal-burning activities, and aerosol precipitation may be another important source of human activities for As. More than half (65.9 %) of the sampling sites were categorized as having low potential ecological risk, 24.4 % for moderate risk, 7.3 % for considerable risk, and 2.4 % for high potential ecological risk, and the potential ecological risk metals of Cr, Cd, and As in NSCS should be more attention.

Long-Term Temporal and Spatial Monitoring of Cladophora Blooms in Qinghai Lake Based on Multi-Source Remote Sensing Images

With climate warming and intensification of human activities, the eco-environmental problems of lakes in middle and high latitudes become increasingly prominent. Qinghai Lake, located in the northeastern of the Tibetan Plateau, is the largest inland saltwater lake in China. Recently, the problem of Cladophora blooms has been widely concerning. In this study, the area of floating Cladophora blooms (hereafter FCBs) in Qinghai Lake from 1986 to 2021 was extracted using Floating Algal Index (FAI) method based on Landsat TM/ETM+/OLI and Sentinel-2 MSI images, and then the intra- and inter-annual variation characteristics and spatial patterns of FCBs were analyzed. The results show that the general change trend of FCBs in Qinghai Lake featured starting in May, expanding rapidly from June to August, and increasing steadily from September to October. From 1986 to 2021, the area of FCBs in Qinghai Lake showed an overall increasing trend in all months, with the largest increase in July at 0.1 km2/a, followed by October at 0.096 km2/a. Spatially speaking, the FCBs area showed a significant increasing trend in the northern Buha River estuary (BRN) and southern Buha River estuary (BRS) regions, a slight increase in the Shaliu River estuary (SR) region, and a decreasing trend in the Quanji River estuary (QR) region and the Heima River estuary (HR) region. The correlation between the meteorological factors and the changes in FCBs was weak, but the increase in flooded pastures in the BRN region (Bird Island) due to rising water levels was definitely responsible for the large-scale increase in FCBs in this region. However, the QB, northeastern bay of Shaliu River estuary (SRB) and HR regions, which also have extensive inundated grassland, did not have the same increase in FCBs area, suggesting that the growth of Cladophora is caused by multiple factors. The complex relationships need to be verified by further research. The current control measures have a certain inhibitory effect on the Cladophora bloom in Qinghai Lake because the FCBs area was significantly smaller in 2017–2020 (5.22 km2, 3.32 km2, 4.55 km2 and 2.49 km2), when salvage work was performed, than in 2016 and 2021 (8.67 km2 and 9.14 km2), when no salvage work was performed.

Effect of Population Migration and Socioeconomic Factors on the COVID-19 Epidemic at County Level in Guangdong, China

Coronavirus disease 2019 (COVID-19) has become a major public health concern worldwide. In this study, we aimed to analyze spatial clusters of the COVID-19 epidemic and explore the effects of population emigration and socioeconomic factors on the epidemic at the county level in Guangdong, China. Data on confirmed cases, population migration, and socioeconomic factors for 121 counties were collected from 1 December 2019 to 17 February 2020, during which there were a total of 1,328 confirmed cases. County-level infected migrants of Guangdong moving from Hubei were calculated by integrating the incidence rate, population migration data of Baidu Qianxi, and the resident population. Using the spatial autocorrelation method, we identified high-cluster areas of the epidemic. We also used a geographical detector to explore infected migrants and socioeconomic factors associated with transmission of COVID-19 in Guangdong. Our results showed that: 1) the epidemic exhibited significant positive global spatial autocorrelation; high–high spatial clusters were mainly distributed in the Pearl River Estuary region; 2) city-level population migration data corroborated with the incidence rate of each city in Hubei showed significant association with confirmed cases; 3) in terms of potential factors, infected migrants greatly contributed to the spread of COVID-19, which has strong ability to explain the COVID-19 epidemic; besides, the companies, transport services, residential communities, restaurants, and community facilities were also the dominant factors in the spread of the epidemic; 4) the combined effect produced by the intersecting factors can increase the explanatory power. The infected migrant factor interacted strongly with the community facility factor with the q value of 0.895. This indicates that the interaction between infected migrants and community facilities played an important role in transmitting COVID-19 at the county level.

Floating marine debris mitigation by vessel routing modeling and optimization considering carbon emission and travel time

Macro-marine debris becomes a global thorny issue to human beings and people are keeping trying every means to mitigate the risk of it. In this paper, we propose to use vessel and develop optimal vessel routing network to collect macro-marine debris on the nearshore surface. Due to the integrated force of winds and ocean currents, debris constantly changes floating location in the ocean. Advanced remote sensing technology and General National Oceanic and Atmospheric Administration (NOAA) Operational Modeling Environment (GNOME) software are employed to identify debris locations and track the drifting trajectory, respectively. In order to balance collecting efficiency and vessel emission reduction, we propose a bi-objective mixed integer nonlinear programming model for vessel routing to minimize travel time and carbon emission, considering time window at debris location, vessel capacity, low/medium/high vessel speed, and cost including carbon tax. A novel pheromone heuristic adaptive large neighborhood search (PHALNS ) algorithm combined with archived multi-objective simulated annealing (AMOSA) mechanism is developed to solve the proposed model. The Yangtze River Estuary region is taken as a numerical example to verify the proposed model and algorithm. Six criterions are introduced to evaluate the best collection time, and the proposed algorithm is also compared with NSGA II algorithm. The results show that low carbon emission and cost effective could be achieved simultaneously, and vessel speed has larger impact than the route scheme.

Occurrence and abundance of poly- and perfluoroalkyl substances (PFASs) on microplastics (MPs) in Pearl River Estuary (PRE) region: Spatial and temporal variations

Although the occurrence and distribution of various chemicals on microplastics (MPs) has been widely studied, little was known about the concentrations of poly- and perfluoroalkyl substances (PFASs) on MPs. In this study, MPs from eight rivers draining into Pearl River Estuary (PRE) region were collected and analyzed. Higher concentrations of PFASs on MPs (105–9.07 × 103 ng g−1) were found in the drain outlets receiving wastewater from most urbanized cities with large population densities. On the other hand, lower concentrations of PFASs on MPs (10.3–227.8 ng g−1) were found in the drain outlets receiving wastewater mostly from agricultural and forested areas. Specially, 8:2 disubstituted polyfluoroalkyl phosphates (8:2 diPAP) was detected with the highest frequency, in 92.5% of the samples. Furthermore, a positive Spearman correlation was found between 6:2 disubstituted polyfluoroalkyl phosphates (6:2 diPAP) and perfluorotetradecanoic acid (PFTeDA) (rs = 0.621, p = 0.012), indicating they might share similar sources. PFASs on MPs were found to vary significantly with different seasons. Higher concentrations of PFASs on MPs were found in dry seasons, while lower concentrations were observed in wet seasons. The results of this study should be important for the understanding of PFAS occurrence and distribution on MPs and the partitioning mechanism of PFASs on MPs in estuary systems.

Microplastics in sediments from an interconnected river-estuary region

Microplastics are an emerging pollutant of international concern due to its wide distribution through various pathways. Estuary is an important pathway for land microplastics to enter into the oceans by rivers. In this study, we hypothesized that microplastics would sink into estuary sediment during diffusion and transmission from river before entering into the sea, which results in higher accumulation of microplastics in proximity to river-estuary than in the oceans. In order to demonstrate this hypothesis, sediment samples were collected from an estuary and its two main inputting rivers and the microplastics in these samples were analyzed. In the collected sediment samples, 19 types of polymers, including the three most common polymers (polyethylene, polyethylene terephthalate, and poly(propylene:ethylene)), were identified and confirmed by FT-IR. Eight types of polymers were consistently detected in all samples, while 11 types of polymers were occasionally found in some samples. These microplastics exhibited four shapes and their percentages followed the high-to-low order of film, fragment, fiber and pellet. A relatively lower abundance of microplastics was found in river sediments from Shuangtaizi River with an average of 170 ± 96 particles/kg d.w., compared to that from Daliao River with an average of 237 ± 129 particles/kg d.w., but it was higher than that from Liaohe Estuary with an average of 120 ± 46 particles/kg d.w. Furthermore, the highest concentration of microplastics was found at the mouth of rivers, showing high accumulation where the freshwater and saltwater meet. Results from this study, including the abundance, characteristics and spatial distribution of microplastic pollution in sediments from an interconnected river-estuary system, revealed the fate and distribution of microplastics in the river and estuary environment.

High-Frequency Variations in Pearl River Plume Observed by Soil Moisture Active Passive Sea Surface Salinity

River plumes play an important role in the cross-margin transport of phytoplankton and nutrients, which have profound impacts on coastal ecosystems. Using recently available Soil Moisture Active Passive (SMAP) sea surface salinity (SSS) data and high-resolution ocean color products, this study investigated summertime high-frequency variations in the Pearl River plume of China and its biological response. The SMAP SSS captures the intraseasonal oscillations in the offshore transport of the Pearl River plume well, which has distinct 30–60 day variations from mid-May to late September. The offshore transport of freshwater varies concurrently with southwesterly wind anomalies and is roughly in phase with the Madden–Julian Oscillation (MJO) index in phases 1–5, thus implying that the MJO exerts a significant influence. During MJO phases 1–2, the southwest wind anomalies in the northeastern South China Sea (SCS) enhanced cross-shore Ekman transport, while the northeast wind anomalies during MJO phases 3–5 favored the subsequent southwestward transport of the plume. The high chlorophyll-a concentration coincided well with the low-salinity water variations, emphasizing the important role of the offshore transport of the Pearl River plume in sustaining biological production over the oligotrophic northern SCS. The strong offshore transport of the plume in June 2015 clearly revealed that the proximity of a cyclonic eddy plays a role in the plume’s dispersal pathway. In addition, heavy rainfall related to the landfall of tropical cyclones in the Pearl River Estuary region contributed to the episodic offshore transport of the plume.

Mapping Annual Land Use and Land Cover Changes in the Yangtze Estuary Region Using an Object-Based Classification Framework and Landsat Time Series Data

A system understanding of the patterns, causes, and trends of long-term land use and land cover (LULC) change at the regional scale is essential for policy makers to address the growing challenges of local sustainability and global climate change. However, it still remains a challenge for estuarine and coastal regions due to the lack of appropriate approaches to consistently generate accurate and long-term LULC maps. In this work, an object-based classification framework was designed to mapping annual LULC changes in the Yangtze River estuary region from 1985–2016 using Landsat time series data. Characteristics of the inter-annual changes of LULC was then analyzed. The results showed that the object-based classification framework could accurately produce annual time series of LULC maps with overall accuracies over 86% for all single-year classifications. Results also indicated that the annual LULC maps enabled the clear depiction of the long-term variability of LULC and could be used to monitor the gradual changes that would not be observed using bi-temporal or sparse time series maps. Specifically, the impervious area rapidly increased from 6.42% to 22.55% of the total land area from 1985 to 2016, whereas the cropland area dramatically decreased from 80.61% to 55.44%. In contrast to the area of forest and grassland, which almost tripled, the area of inland water remained consistent from 1985 to 2008 and slightly increased from 2008 to 2016. However, the area of coastal marshes and barren tidal flats varied with large fluctuations.

DDT exposure induces cell cycle arrest and apoptosis of skin fibroblasts from Indo-Pacific humpback dolphin via mitochondria dysfunction

Although the global use of the 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (p,p’-DDT) has been prohibited, its persistence in the environment has caused long-lasting exposure on marine mammals. Our previous studies revealed exceedingly high residue levels of DDTs in Indo-Pacific humpback dolphins (Sousa chinensis) from the Pearl River Estuary region, China. However, the molecular mechanisms of p,p’-DDT toxicity on the dolphin are largely unknown. This study conducted the first cytotoxicity effect exploration of p,p’-DDT on the dolphin skin fibroblasts (ScSFs) to enhance the understanding of the cellular and molecular regulation impacts. ScSF cells were exposed to p,p’-DDT (28∼168 μM) for 24, 48 and 72 h. The exposure remarkably decreased viability of ScSF cells, possibly due to the synergetic effects of cell cycle arrest and apoptosis via DNA damage and mitochondria dysfunction. The DNA damage and mitochondria dysfunction were likely triggered by an increase of cellular reactive oxygen species (ROS), alteration in mitochondrial membrane potential, reduction in the cellular ATP levels, decreased expression of the genes CDK1, CDK4, cyclin B1, cyclin D1 and apoptosis regulator Bcl-2, release of cytochrome c, and activation of caspase-3, caspase-8 and caspase-9. Moreover, caspase inhibitor displayed protective activity against p,p’-DDT-induced apoptosis, indicating that caspases played a central role in p,p’-DDT-triggered apoptosis in the ScSF cells. We hypothesize apoptosis likely plays a minor role in cytocidal effects induced by p,p’-DDT exposure, but the mechanisms remain unclear. Overall, this research provides new evidence of the cytotoxic mechanisms underlying p,p’-DDT exposure on humpback dolphin skin cells, and suggests that p,p’-DDT contamination is one of key health concern issues for the protection of this marine mammal.

A blended inherent optical property algorithm for global satellite ocean color observations

AbstractWater inherent optical properties (IOPs) can be derived from satellite‐measured normalized water‐leaving radiance (nLw(λ)) spectra. In this study, we evaluate the performance of the quasi‐analytical algorithm (QAA) and the near‐infrared (NIR)‐based IOP algorithm using a Hydrolight simulation data set covering a wide range of water types that span from clear open ocean to turbid coastal/inland waters. The NIR‐based algorithm produces significantly improved IOP retrievals over turbid coastal and inland waters, while the QAA algorithm performs well in the open ocean and less turbid coastal waters. Based on the advantages of the NIR‐based and QAA‐based algorithms, a combination of the NIR‐ and QAA‐based algorithm has been proposed using satellite‐measured nLw(745) as the threshold in order to produce accurate IOP products for both the open ocean and turbid coastal/inland waters. The new combined IOP algorithm can produce reasonably accurate IOP data for all water types, and can be easily implemented into the satellite ocean color data processing. The La Plata River Estuary region is used as an example to show the difference in performance of IOP retrievals from the Visible Infrared Imaging Radiometer Suite (VIIRS) measurements between 2012 and 2017 with the NIR‐based, QAA‐based, and NIR‐QAA combined IOP algorithms. We also demonstrate that the NIR‐QAA combined algorithm can be applied to VIIRS global ocean color observations to derive good quality IOP products in China's east coastal region, the US east coastal region, and the region of Mississippi River Estuary and tributaries.

The genus Amphorotia (Bacillariophyta) in subtropical Asian coastal areas: Species diversity in fossil material from the Pearl River system, China

ABSTRACTAmphorotia is a diatom genus of considerable morphological and biogeographical significance. It was established by transferring several Eunotia species and describing several new species. The most species were found dispersed towards Southeast Asia. In coastal areas of China, a number of species of Amphorotia were found and the assemblages contained several new taxa. This article provides additional information on five previously described species and describes one new species, Amphorotia zhujiangensis sp. nov., and one uncertain but special species, A. sp. These are based on fossil material from a deltaic borehole, subaquatic sediment cores and surface sediments collected in the Pearl River estuary region. Morphological data were derived from light and scanning electron microscopy. A detailed morphological comparison of seven Amphorotia species is included, indicating the existence of a diverse group in brackish waters. Relationships within the family Eunotiaceae are briefly discussed. The biogeographical range of regional habitats occupied by Amphorotia species in tropical Southeast Asia is also expanded.

Hexavalent chromium induces oxidative stress and mitochondria-mediated apoptosis in isolated skin fibroblasts of Indo-Pacific humpback dolphin

The increasing gas emissions and industrial wastewater discharge of anthropogenic hexavalent chromium (Cr(VI)) have been growing health concerns to the high trophic level marine mammals. Our previous studies showed that Indo-Pacific humpback dolphin (Sousa chinensis), stranded on the Pearl River Estuary region, contained exceedingly high levels of Cr in their skin-tissues. Unfortunately, the molecular toxic mechanisms on this mammal are absent, limiting our understanding of the eco-physiological impacts of Cr(VI) on dolphins. Thus, the cytotoxicity effects of Cr(VI) were analyzed on fibroblasts we isolated from the skin of S. chinensis (ScSF). This study showed that Cr(VI) markedly inhibited the viability of ScSF cells via induction of apoptosis accompanied by an increase in the production of reactive oxygen species and the population of G2/M arrest or apoptotic sub-G1 phase cells, up-regulation of p53, and activation of caspase-3. Further investigation on intracellular mechanisms indicated that Cr(VI) induced depletion of mitochondrial membrane potential in cells through regulating the expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) proteins, resulting in decrease of the ATP level, cytochrome c release from mitochondria into cytosol, and the activation of caspase-9. Furthermore, antioxidants N-acetylcysteine and vitamin C displayed chemoprotective activity against Cr(VI) via suppression of p53 expression, indicating that the Cr(VI)-induced cell death may be mediated by oxidative stress. Overall, these results provide insights into the potential mechanisms underlying the cytotoxicity of Cr(VI) in Indo-Pacific humpback dolphin skin cells, offer experimental support for the proposed protective role of antioxidants in Cr(VI)-induced toxicity, and suggest that Cr(VI) contamination is one of key health concern issues for the protection of Indo-Pacific humpback dolphin.

The Monitoring of Topography Change in the Estuary of the Korean East Coast Using LiDAR

ABSTRACT Kim, S.; Lee, H.; Jun, K., and Jun, B.-H., 2018. The monitoring of topography change in the estuary of the Korean east coast using LiDAR. In: Shim, J.-S.; Chun, I., and Lim, H.S. (eds.), Proceedings from the International Coastal Symposium (ICS) 2018 (Busan, Republic of Korea). Journal of Coastal Research, Special Issue No. 85, pp. 46–50. Coconut Creek (Florida), ISSN 0749-0208. Recently, due to abnormal climates caused by climate changes, important shorelines found in the east coast of Korea are facing many topographical changes. Especially within the estuary regions that meet the rivers, complex phenomenon due to artificial causes like topographical and stream character changes are occurring. In this study, GPS with RTK (Real Time Kinematic) application and above ground LiDAR (Light Detection And Ranging) were used to observe and the changing typography by season of Gagok River estuary region located in the east coast of Korea. Also the changes in water discharge from the Gagok River were measu...

Interannual sea level variability in the Pearl River Estuary and its response to El Niño–Southern Oscillation

Abstract The South China coast, especially the Pearl River Estuary (PRE) region, is prosperous and densely populated, but vulnerable to sea level changes. Sea level anomalies (SLA) during 1954–2012 from tide gauge station data and regional SLAs during 1993–2012 from satellite altimetry are analyzed and compare to the El Nino–Southern Oscillation (ENSO). Results show that sea level declines during El Nino events and rises during La Nina. Sea level in the PRE responds to ENSO with ~3–month lag. The ENSO can cause sea level in the PRE to fluctuate from −8.70 to 8.11 cm. Sea level cycles of 3 and 5 years are related to ENSO. The ENSO mechanism affecting sea level in the PRE was analyzed by identifying dominant regional and local forces. Weak/strong SLAs in most El Nino/La Nina events may be attributed to less/more seawater transport driven by anomalously weak/strong north winds and local anomalously high/low sea level pressure. Wind-driven coastal current is the predominant factor. It generated coastal seawater volume transport along a ~160 km wide cross section to decrease by 21.07% in a typical El Nino period (January 2010) and increase by 44.03% in a typical La Nina period (January 2011) as compared to an ENSO neutral situation (January 2013). Results of sea level rise and its potential mechanism provide insight for disaster protection during extreme El Nino/La Nina events.