Currently, the operational risk assessment of quay crane operators at ports relies on manual evaluations based on experience, but this method lacks objectivity and fairness. As port throughput continues to grow, the port accident rate has also increased, making it crucial to scientifically evaluate the risk behaviors of operators and improve their safety awareness. This paper proposes an automated evaluation method based on a Deep Q-Network (DQN) to assess the risk behaviors of quay crane operators in virtual scenarios. A risk simulation module has been added to the existing automated quay crane remote operation simulation system to simulate potential risks during operations. Based on the collected data, a DQN-based benchmark model reflecting the operational behaviors and decision-making processes of skilled operators has been developed. This model enables a quantitative evaluation of operators’ behaviors, ensuring the objectivity and accuracy of the assessment process. The experimental results show that, compared with traditional manual scoring methods, the proposed method is more stable and objective, effectively reducing subjective biases and providing a reliable alternative to conventional manual evaluations. Additionally, this method enhances operators’ safety awareness and their ability to handle risks, helping them identify and avoid risks during actual operations, thereby ensuring both operational safety and efficiency.