Surface sediments collected in 2021 from six locations in the southern Baltic Sea (Polish district) were examined by chemical and toxicological methods. Chemical analyses included polybrominated diphenyl ethers (PBDEs), polycyclic aromatic hydrocarbons (PAHs), and their alkylated derivatives, butyltin compounds and 16 major and trace elements. The toxicity was measured using Ostracodtoxkit F and Microtox. The ecological risk of sediment was estimated by hazard quotient (HQ) calculation. Some PAHs, alkylated PAHs and metals (Zn, Hg, Cd and As) could pose a moderate risk in the sediments from the Gdańsk Deep and in the vicinity of the wrecks, but the risk resulting from the presence of all analyzed compounds was considered high for these sediments. In studies using biotests, sediments from the vicinity of the t/s Franken wreck and the Słupsk Furrow were highly toxic to test organisms. Ostracodtoxkit F, compared to Microtox, appeared a more sensitive test for measured compounds.