Rigid Tail Research Articles

Experimental study on flow boiling heat transfer in rigid-tail rib-channel under rolling motion conditions

This study proposes a novel addition of rigid filamentous tail structure at the trailing edge of the ribs to enhance the heat transfer performance of flow boiling in mini-channel under rolling conditions. The flow boiling heat transfer characteristics of Novec649 in both rib-channel and rigid-tail rib-channel are experimentally investigated. Heat flux, inlet flow velocity, rolling angle and rolling period are considered as the variable parameters, which are 50 – 300 kW·m−2, 0.3 – 0.9 m·s−1, 0 – 15°, 10 – 20 s, respectively. The experimental results show that the rigid-tail structure improves the heat transfer performance of the channels within the entire range of thermal parameters, while increasing the pressure drop at high heat flux and/or high velocity. Compared with rib-channel, the rigid-tail structure exhibits the strongest enhancement in flow boiling heat transfer performance under high heat flux of qeff = 300 kW·m−2 and high flow velocity of vin = 0.9 m·s−1, and the heat transfer coefficient can be increased by 6.93 % at most. And increasing the rolling angle or decreasing the rolling period will decrease the average wall temperature by a maximum of 1.94 °C and increase the heat transfer performance factor j by a maximum of 8 % at vin = 0.3 m·s−1, qeff = 200 – 300 kW·m−2 for the rigid-tail rib-channel. Furthermore, the addition of a rigid-tail structure helps maintain smaller bubble sizes and a more uniform distribution in the mainstream region of the two-phase flow.

In vitro antimicrobial and antibiofilm activity of phage cocktail against Mammaliicoccus sciuri, a causative agent of bovine mastitis.

In this research paper the in vitro antimicrobial and antibiofilm activity of phage cocktail against the coagulase negative Mammaliicoccus sciuri was investigated. Three M. sciuri isolates obtained from clinical bovine mastitis samples were characterized and identified by 16S rRNA gene sequencing. Bacteriophages with lytic activity against M. sciuri isolates were isolated from dairy farm effluents. Two typical phages were isolated using standard enrichment and plaque assay techniques, purified by polyethylene glycol precipitation, and morphologically characterized based on shape and size using transmission electron microscopy. This was followed by determination of host range using spot tests and stability to varying temperature, pH and UV treatment. The phage cocktail suppressed bacterial activity within 30 min of exposure. Crystal violet assay showed that the tested phages and their cocktail significantly reduced the biofilm biomass of all three M. sciuri strains compared to the untreated control in vitro within 24 h with a single dosing. Transmission electron micrography of the purified phage particle revealed an icosahedral head and a rigid contractile tail, characteristic of the class Caudoviricetes. The findings open new avenues in phage-based antimicrobial approaches for controlling contagious and teat skin opportunistic bacteria causing bovine mastitis.

The effect of tail stiffness on a sprawling quadruped locomotion.

A distinctive feature of quadrupeds that is integral to their locomotion is the tail. Tails serve many purposes in biological systems, including propulsion, counterbalance, and stabilization while walking, running, climbing, or jumping. Similarly, tails in legged robots may augment the stability and maneuverability of legged robots by providing an additional point of contact with the ground. However, in the field of terrestrial bio-inspired legged robotics, the tail is often ignored because of the difficulties in design and control. In this study, we test the hypothesis that a variable stiffness robotic tail can improve the performance of a sprawling quadruped robot by enhancing its stability and maneuverability in various environments. In order to validate our hypothesis, we integrated a cable-driven, flexible tail with multiple segments into the underactuated sprawling quadruped robot, where a single servo motor working alongside a reel and cable mechanism regulates the tail's stiffness. Our results demonstrated that by controlling the stiffness of the tail, the stability of locomotion on rough terrain and the climbing ability of the robot are improved compared to the movement with a rigid tail and no tail. Our findings highlight that constant ground support provided by the flexible tail is key to maintaining stable locomotion. This ensured a predictable gait cycle, eliminating unexpected turning and slipping, resulting in an increase in locomotion speed and efficiency. Additionally, we observed the robot's enhanced climbing ability on surfaces inclined up to 20°. The flexibility of the tail enabled the robot to overcome obstacles without external sensing, exhibiting significant adaptability across various terrains.

The saclayvirus Aci01-1 very long and complex fiber and its receptor at the Acinetobacter baumannii surface.

The Acinetobacter baumannii bacteriophage Aci01-1, which belongs to the genus Saclayvirus of the order Caudoviricetes, has an icosahedral head and a contractile rigid tail. We report that Aci01-1 has, attached to the tail conical tip, a remarkable 146-nm-long flexible fiber with seven beads and a terminal knot. Its putative gene coding for a 241.36-kDa tail fiber protein is homologous to genes in Aci01-1-related and unrelated phages. Analysis of its 3D structure using AlphaFold provides a structural model for the fiber observed by electron microscopy. We also identified a putative receptor of the phage on the bacterial capsule that is hypothesized to interact with the Aci01-1 long fiber.

Structure and proposed DNA delivery mechanism of a marine roseophage

Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.

In Situ Structures of the Ultra-Long Extended and Contracted Tail of Myoviridae Phage P1.

The Myoviridae phage tail is a common component of contractile injection systems (CISs), essential for exerting contractile function and facilitating membrane penetration of the inner tail tube. The near-atomic resolution structures of the Myoviridae tail have been extensively studied, but the dynamic conformational changes before and after contraction and the associated molecular mechanism are still unclear. Here, we present the extended and contracted intact tail-structures of Myoviridae phage P1 by cryo-EM. The ultra-long tail of P1, 2450 Å in length, consists of a neck, a tail terminator, 53 repeated tail sheath rings, 53 repeated tube rings, and a baseplate. The sheath of the contracted tail shrinks by approximately 55%, resulting in the separation of the inner rigid tail tube from the sheath. The extended and contracted tails were further resolved by local reconstruction at 3.3 Å and 3.9 Å resolutions, respectively, allowing us to build the atomic models of the tail terminator protein gp24, the tube protein BplB, and the sheath protein gp22 for the extended tail, and of the sheath protein gp22 for the contracted tail. Our atomic models reveal the complex interaction network in the ultra-long Myoviridae tail and the novel conformational changes of the tail sheath between extended and contracted states. Our structures provide insights into the contraction and stabilization mechanisms of the Myoviridae tail.

Tail-stiffness optimization for a flexible robotic fish

Undulation regulation in a robotic fish propelled by a passive flexible tail is more similar to that of a natural fish than with a rigid tail, owing to the smooth curvature of the flexible tail. Moreover, it has been observed that fish change the stiffness of their bodies to adapt to various swimming states. Inspired by this, a stiffness optimization scheme is explored for a novel elastic tail, which can improve the performance of the robotic fish. Spring steels are used as passive flexible joints of the fishtail; these can be easily expanded into multi-joint structures and the joint stiffness can be altered by changing the joint size. In this study, the Lagrangian dynamic method is employed to establish a dynamic model of the robotic fish in which passive flexible joints are simplified by a pseudo-rigid-body model. In addition, the hydrodynamics of the head and tail are analyzed using the simplified Morison equation and quasi-steady wing theory, respectively. Furthermore, to determine unknown hydrodynamic parameters in the dynamic model, a parameter identification method is applied. The results show that the identified simulation speeds fit the experimental speeds well within a wide range of stiffness values. Finally, to improve performance, the influence of joint stiffness and frequency on swimming speed is investigated based on the identified dynamic model. At each frequency, the optimal joint stiffness distribution is one that reduces the stiffness from the front to the rear. At the maximum driving frequency of 2.5 Hz, the optimal swimming speed is 0.3 body lengths per second, higher than that when rigid joints are used.

Chordwise flexible aft-tail suppresses jet-switching by reinstating wake periodicity in a flapping foil

The effect of a chordwise flexible aft-tail of a rigid heaving aerofoil on the dynamical transitions of the trailing-wake is studied here. The two-way coupled fluid–solid dynamics is simulated using an in-house fluid–structure interaction (FSI) platform, comprising a discrete forcing immersed boundary method based incompressible Navier–Stokes solver, weakly coupled with a finite difference method based structural solver. The FSI dynamics is studied in comparison to the corresponding rigid tail configuration. For the latter, mild jet-switching due to quasi-periodic movement of the wake vortices gives way to vigorous jet-switching as the dynamics transitions to a state of intermittency, where the quasi-periodic behaviour gets interspersed with chaotic windows. Introduction of a moderately flexible tail regularises this intermittent dynamics, eliminating jet-switching. The wake exhibits a deflected reverse Kármán pattern with fluctuating angles, governed by quasi-periodicity. With a highly flexible tail (very low rigidity), the wake shows almost a symmetric reverse Kármán street as periodicity is restored. Flexibility of the aft-tail is next controlled by changing its length, and flow is regularised and periodicity retained for moderate rigidity for increased length. Different dynamical states are established through robust nonlinear dynamical tools. The underlying flow-field behaviour, instrumental in suppressing the jet-switching phenomenon, is identified through a detailed investigation of the near-field vortex interactions dictated by the dynamics. A suite of measures has also been derived from the unsteady flow field to quantify the interactions of the key near-field vortices with a view to understanding the mechanism of switching and its subsequent suppression through flexibility.

Scorpion-Shaped Zinc Porphyrins as Tetrafunctional TAR RNA Predators and HIV-1 Reverse Transcriptase Inhibitors.

HIV-1 reverse transcriptase (RT) inhibitors are fundamental to the discovery and development of anti-HIV drugs. Their main target is RT, and only a tiny number of them can bind to viral RNA. In this paper, five new Zn(II) porphyrin compounds were developed with different characters. ZnTPP4 has both the appearance and the functions of a scorpion with a rigid tail and stinger to selectively hunt HIV-1 TAR RNA based on the molecular recognition of hydrogen bonds, a fierce chelicera to bite RNA by metal coordination, mighty pedipalps to grasp the bound RNA by supramolecular inclusion, and a broad body maintaining the configuration of each functional area so that they can cooperate with each other and providing accommodation space for the bound RNA. This tetrafunctional Zn(II) porphyrin is relatively nontoxic to normal cells and can produce sensitive responses for RNA. Moreover, this work offers practical construction methodologies for medication of AIDS and other diseases closely related to RT like EBOV and SARS-CoV-2.

New theropod dinosaur from the Late Cretaceous of Brazil improves abelisaurid diversity

The Late Cretaceous Marília Formation (Bauru Group, Bauru Basin) is a geological unit that occurs on São Paulo, Minas Gerais, Goiás, and Mato Grosso do Sul states, Brazil. This formation consists predominantly of paleosols developed in a semiarid/arid environment and recent reappraisal of its formerly known members reduced its lithological composition and geographical distribution. Hence, the Marília Formation has a very sparse vertebrate fossil record without named species so far. In this contribution we describe a new abelisaurid theropod (Dinosauria) from this unit, namely Kurupi itaata gen. et sp. nov., discovery in the Municipality of Monte Alto, western São Paulo State. The holotype MPMA 27-0001/02 consists of three caudal vertebrae and the partial pelvic girdle. Kurupi itaata gen. et sp. nov. shares with other South American abelisaurids fused ischia and caudal vertebrae with long and laterodorsally oriented transverse processes, with fan-shaped distal ends. Autapomorphies of the new theropod include the variation of ∼15° of the inclination of the transverse process between the first (C1) and seventh (C7) caudal vertebra; C1 with anterolaterally projected triangular process placed below the proximodistal mid-portion of the transverse process, and a notch at the anterodistal portion of the transverse process, between a shelf-like process and the anterodistal corner of the transverse process; and anterior caudal vertebrae (present in C1 and C7, inferred in the others) with a cuneiform process, anterodorsally projected, located on the dorsal surface of the transverse process. Phylogenetic analysis recovered Kurupi itaata gen. et sp. nov. among abelisaurid theropods, but was nested in an unresolved massive polytomy of the entire clade. Taphonomic traits on the studied specimens corroborate previous proposals for the paleoenvironmental context of the Marília Formation. Kurupi itaata gen. et sp. nov. was about 5 m long, with a rigid tail, and cursorial locomotion as indicated by its a muscles attachment and bones anatomy. This new taxon contributes to the knowledge of the Maastrichtian continental fauna of Brazil and increases the diversity of medium-sized abelisaurids in western Gondwana.

Modeling and linearization of longitudinal dynamics for a flapping wing micro aerial vehicle dragonfly-like with active rigid tail

The main purpose of this paper is to model and simulate flight dynamics for a flapping wing micro aerial vehicle dragonfly-like with two pair clap and fling mechanism and active rigid tail. This article simulates the flight dynamics of a micro aerial vehicle dragonfly-like that can also use tail movements for longitudinal stability. Initially, using Kane's method, the equations of motion of the longitudinal mode are obtained. Then aerodynamics forces of Delfly II micro aerial vehicle and gearbox simulation are added to the equations of motion. Also, a novel design for a flapping wing micro aerial vehicle dragonfly-like is presented, in which tail movement is similar to the movement of insect tails in longitudinal mode. In this work, the tail movement is not used as an elevator, but the rigid tail movement is used as a control torque. The difference in brush motor rpm leads to differential thrust and pitch moment generation, similar to a quadrotor. Finally, the dynamic equations and aerodynamics and gearbox are all linearized and presented as state-space equations. Also, the response of the open-loop linearized model is compared with the nonlinear response by creating suitable initial conditions for the brush motor rpm.

A simplified and modified model for long rod penetration based on ovoids of Rankine

This paper proposes an alternative to the Alekseevskii-Tate model, denoted by AT, for erosion of a long rod penetrator. Specifically, the model uses simplified equations of the model for long rod penetration based on ovoids of Rankine developed in (Roisman et al., 2001). The resulting modified ovoid of Rankine model, denoted by MOR, proposes differential equations for the velocity of the projectile’s tail, the penetration velocity into the target, the erosion of the projectile’s mass and the penetration depth, as well as an algebraic equation to determine the point of separation of the target material from the projectile’s surface. The MOR model uses shock conditions to determine the initial value of the penetration velocity and it predicts the initial transient decrease of this velocity to its value for quasi-steady-state projectile erosion. This model also includes an expression for the crater diameter which predicts values close to experimental data. Relative to the AT model, the MOR model introduces a single empirical parameter in a modified evolution for mass erosion which significantly influences the penetration depth without affecting the crater diameter. This parameter models the interface between the deforming projectile’s nose and its rigid tail where the projectile’s tail is rapidly decelerating due to mushrooming and mass erosion. Its value is determined by comparing model predictions of the penetration depth with experimental data. Future research is needed to replace this empirical parameter with an analytical model and to better model the termination process of penetration.

Identification of PUFA interaction sites on the cardiac potassium channel KCNQ1.

Polyunsaturated fatty acids (PUFAs), but not saturated fatty acids, modulate ion channels such as the cardiac KCNQ1 channel, although the mechanism is not completely understood. Using both simulations and experiments, we find that PUFAs interact directly with the KCNQ1 channel via two different binding sites: one at the voltage sensor and one at the pore. These two amphiphilic binding pockets stabilize the negatively charged PUFA head group by electrostatic interactions with R218, R221, and K316, while the hydrophobic PUFA tail is selectively stabilized by cassettes of hydrophobic residues. The rigid saturated tail of stearic acid prevents close contacts with KCNQ1. By contrast, the mobile tail of PUFA linoleic acid can be accommodated in the crevice of the hydrophobic cassette, a defining feature of PUFA selectivity in KCNQ1. In addition, we identify Y268 as a critical PUFA anchor point underlying fatty acid selectivity. Combined, this study provides molecular models of direct interactions between PUFAs and KCNQ1 and identifies selectivity mechanisms. Long term, this understanding may open new avenues for drug development based on PUFA mechanisms.

Design, Modeling, and Simulation of a Wing Sail Land Yacht

Autonomous land yachts can play a major role in the context of environmental monitoring, namely, in open, flat, windy regions, such as iced planes or sandy shorelines. This work addresses the design, modeling, and simulation of a land yacht probe equipped with a rigid free-rotating wing sail and tail flap. The wing was designed with a symmetrical airfoil and dimensions to provide the necessary thrust to displace the vehicle. Specifically, it proposes a novel design and simulation method for free rotating wing sail autonomous land yachts. The simulation relies on the Gazebo simulator together with the Robotic Operating System (ROS) middleware. It uses a modified Gazebo aerodynamics plugin to generate the lift and drag forces and the yawing moment, two newly created plugins, one to act as a wind sensor and the other to set the wing flap angular position, and the 3D model of the land yacht created with Fusion 360. The wing sail aligns automatically to the wind direction and can be set to any given angle of attack, stabilizing after a few seconds. Finally, the obtained polar diagram characterizes the expected sailing performance of the land yacht. The described method can be adopted to evaluate different wing sail configurations, as well as control techniques, for autonomous land yachts.

Numerical Simulation of a Multi-Body System Mimicking Coupled Active and Passive Movements of Fish Swimming

A multi-body system model is proposed for the mimicking of swimming fish with coupled active and passive movements. The relevant algorithms of the kinematics and dynamics of the multi-body system and coupled fluid solver are developed and fully validated. A simplified three-body model is applied for the investigation of the hydrodynamic performance of both an active pitch motion and passive movement. In general, there is an optimal stiffness, under which the model swims with the fastest velocity. The effect of the damper can be drawn only when the stiffness is small. Comparing with the rigid tail, the flexible tail leads to a faster speed when the stiffness and damping coefficients are in a suitable range.

Dynamic modeling, analysis, and comparative study of a quadruped with bio-inspired robotic tails

Looking to nature, animals frequently utilize tails to work alongside or in place of their legs to maneuver, stabilize, and/or propel to achieve highly agile motions. Although the single-link robotic tail shows its dynamical superiority and practical effectiveness in mobile platform maneuvering, most tails observed in nature have multi-link structures. Therefore, to investigate this novel tail structure, bio-inspired and biomimetic multi-link robotic tails were proposed and implemented. However, due to the lack of a whole-body dynamic model, previous research focused on investigating the tail subsystem independently without considering the mobile platform’s motions, which introduces deficiencies on both analysis and control. To bridge this theoretical gap, this paper presents a unified dynamics model that incorporates both the quadruped and the tail subsystems as a complete coupled dynamic system. Classical multibody dynamics formulation based on the principle of virtual work is utilized to derive the dynamic model. Based on the new whole-body dynamic model, three typical tail structures, including a single-link pendulum tail, a multi-link rigid tail, and a multi-link flexible tail are evaluated. The results indicate that by using a center of mass-based benchmark, the multi-link tail structure is dynamically equivalent to the single-link tail structure for bending motion. However, for rolling motions, the multi-link structure illustrates noticeable dynamical benefits compared to a single-link structure due to its higher inertia. In addition, a multi-link flexible structure shows significant oscillations and uncontrollable dynamic behaviors due to its under-actuation feature, which may limit its usage for highly dynamic applications.

DNA-Origami-Assisted Flow-Aligned Single-Particle Diffractive Imaging using XFEL Pulses

Outrunning radiation damage, highly intense femtosecond pulses of X-ray free-electron lasers (XFELs) open up the possibility of structure determination of macromolecules to viruses at room temperature, by aggregating diffraction patterns recorded from uncrystallized single-particles. A key challenge in XFEL single-particle diffractive imaging (SPI) is to either constrain the orientation of the particle or to determine it from each of the very noisy weak diffraction patterns. Here we report a unique approach to address these challenges using structural DNA nanotechnology. A DNA-origami “rigid tail” is site-specifically attached to the macromolecule to flow-align it in a thin liquid jet, and also provides a strong holographic reference. In a proof-of-principle study, the computational design and production of the DNA-origami-target construct has been achieved and the experimental results obtained from the Linac Coherent Light Source (LCLS), USA show the alignment of the target single-particle, consistent with simulations, at extremely low concentrations approaching single-molecule in the interaction region with the nanofocus hard X-ray laser beam, in a sum of as few as a thousand single-shots. The results open up the possibility of single-molecule diffractive imaging in solution with XFEL pulses. Acknowledgements: The Human Frontier Science Program (RGP0010/2017).

Comparison of the pharmacologic profiles of arginine vasopressin and oxytocin analogs at marmoset, titi monkey, macaque, and human oxytocin receptors

The oxytocin-arginine vasopressin (OT-AVP) ligand-receptor family influences a variety of physiological, behavioral, and social behavioral processes in the brain and periphery. The OT-AVP family is highly conserved in mammals, but recent discoveries have revealed remarkable diversity in OT ligands and receptors in New World Monkeys (NWMs) providing a unique opportunity to assess the effects of genetic variation on pharmacological signatures of peptide ligands. The consensus mammalian OT sequence has leucine in the 8th position (Leu8-OT), whereas a number of NWMs, including the marmoset, have proline in the 8th position (Pro8-OT) resulting in a more rigid tail structure. OT and AVP bind to OT’s cognate G-protein coupled receptor (OTR), which couples to various G-proteins (Gi/o, Gq, Gs) to stimulate diverse signaling pathways. CHO cells expressing marmoset (mOTR), titi monkey (tOTR), macaque (qOTR), or human (hOTR) OT receptors were used to compare AVP and OT analog-induced signaling. Assessment of Gq-mediated increase in intracellular calcium (Ca2+) demonstrated that AVP was less potent than OT analogs at OTRs from species whose endogenous ligand is Leu8-OT (tOTR, qOTR, hOTR), relative to Pro8-OT. Likewise, AVP-induced membrane hyperpolarization was less potent at these same OTRs. Evaluation of (Ca2+)-activated potassium (K+) channels using the inhibitors apamin, paxilline, and TRAM-34 demonstrated that both intermediate and large conductance Ca2+-activated K+ channels contributed to membrane hyperpolarization, with different pharmacological profiles identified for distinct ligand-receptor combinations. Understanding more fully the contributions of structure activity relationships for these peptide ligands at vasopressin and OT receptors will help guide the development of OT-mediated therapeutics.

Design, Analysis, and Integration of a New Two-Degree-of-Freedom Articulated Multi-Link Robotic Tail Mechanism

Abstract Based on observations from nature, tails are believed to help animals achieve highly agile motions. Traditional single-link robotic tails serve as a good simplification for both modeling and implementation purposes. However, this approach cannot explain the complicated tail behaviors exhibited in nature where multi-link structures are more commonly observed. Unlike its single-link counterpart, articulated multi-link tails essentially belong to the serial manipulator family which possesses special motion transmission design challenges. To address this challenge, a cable-driven hyper-redundant design becomes the most used approach. Limited by cable strength and elastic components, this approach suffers from low-frequency response, inadequate generated inertial loading, and fragile hardware, which are all critical drawbacks for robotic tails design. To solve these structure-related shortcomings, a multi-link robotic tail made up of rigid links is proposed in this paper. The new structure takes advantage of the traditional hybrid mechanism architecture, but utilizes rigid mechanisms to couple the motions between the ith link and the (i + 1)th link rather than using cable actuation. By doing so, the overall tail becomes a rigid mechanism that achieves quasi-uniform spatial bending for each segment and allows performing highly dynamic motions. The mechanism and detailed design of this new robotic tail are presented. The kinematic model was developed and an optimization process was conducted to reduce the bending non-uniformity for the rigid tail. Based on this special optimization design, the dynamic model of the new mechanism is significantly simplified. A small-scale three-segment prototype was integrated to verify the proposed mechanism's unique mobility.

A novel jumbo Tenacibaculum maritimum lytic phage with head-fiber-like appendages.

A novel jumbo bacteriophage (myovirus) is described. The lytic phage of Tenacibaculum maritimum, which is the etiological agent of tenacibaculosis in a variety of farmed marine fish worldwide, was plaque-isolated from seawater around a fish aquaculture field in Japan. The phage had an isometric head 110-120 nm in diameter, from which several 50- to 100-nm-long flexible fiber-like appendages emanate, and a 150-nm-long rigid contractile tail. The full genomes of the two representative phages (PTm1 and PTm5) were 224,680 and 226,876 bp long, respectively, both with 29.7% GC content, and the number of predicted open reading frames (ORFs) was 308 and 306, respectively. The average nucleotide sequence identity between PTm1 and PTm5 was 99.95%, indicating they are quite similar to each other. A genetic relationship was found in 15.0-16.6% of the predicted ORFs among the T. maritimum phages PTm1 and PTm5, the Tenacibaculum spp. phage pT24, and the Sphingomonas paucimobilis phage PAU. Phylogenetic analysis based on the terminase large subunit genes revealed that these four phages (PTm1, PTm5, pT24 and PAU) are more closely related than the other 10 jumbo myoviruses that have similar genome sizes. Transmission electron microscopy observations suggest that the head fibers of the T. maritimum phage function as tentacles to search and recognize the host cell surface to facilitate infection.