Rich Unsaturated Fatty Acid Research Articles

Mitochondrial genome study of Camellia oleifera revealed the tandem conserved gene cluster of nad5-nads in evolution.

Camellia oleifera is a kind of high-quality oil supply species. Its seeds contain rich unsaturated fatty acids and antioxidant active ingredients, which is a kind of high-quality edible oil. In this study, we used bioinformatics methods to decipher a hexaploid Camellia oil tree's mitochondrial (mt) genome based on second-generation sequencing data. A 709,596 bp circular map of C. oleifera mt genome was found for the first time. And 74 genes were annotated in the whole genome. Mt genomes of C. oleifera and three Theaceae species had regions with high similarity, including gene composition and gene sequence. At the same time, five conserved gene pairs were found in 20 species. In all of the mt genomes, most of nad genes existed in tandem pairs. In addition, the species classification result, which, according to the gene differences in tandem with nad5 genes, was consistent with the phylogenetic tree. These initial results provide a valuable basis for the further researches of Camellia oleifera and a reference for the systematic evolution of plant mt genomes.

The Effect of Okra Seed (Abelmoschus esculentus) Powder Supplementation on Nutritional, Textural, Microstructural, and Sensory Properties of Gluten‐Free Muffins

Celiac disease is an autoimmune‐mediated systemic disorder that develops in those who are genetically predisposed, the management of which is limited to a lifelong gluten‐free diet. Gluten‐free foods contain fewer proteins, dietary fiber, and minerals than their gluten counterparts. Among the appropriate ingredients, okra seed is known for its high protein, fat, and dietary fiber content, its well‐balanced amino acid composition, and its rich unsaturated fatty acid profile. In the present study, muffins were prepared with part of the rice flour substituted by okra seed powder (OSP) (15, 30, and 45%) and the nutritional, textural, microstructural, and sensory properties of the gluten‐free muffins were evaluated. The most preferred formulation was determined using the integrated SWARA‐TOPSIS multicriteria decision‐making method, revealing the 30% OSP‐added muffin to be the optimum alternative, with 2.43% ash, 28.01% fat, 10.0% protein, 7.39% total dietary fiber, and 49.75 mg GAE/100 g total phenolic content. In a mineral matter analysis, the Mg, P, K, Ca, Mn, Fe, and Zn contents of the 30% OSP‐added sample were found to be 4.3, 1.2, 2.3, 2.7, 1.4, 4.9, and 2.0 times higher than the control sample, respectively. The OSP‐added gluten‐free muffin samples were also found to be an important source of essential and nonessential amino acids and a good source of linoleic and oleic acids. All of the muffin samples received acceptable sensory scores (>4/7). A texture profile analysis revealed that the hardness, adhesiveness, gumminess, and chewiness values of the muffins increased with the supplementation of OSP, while scanning electron microscope imaging revealed a homogeneous pore structure in the control samples that decreased as the OSP substitution rate increased. The results of the study revealed OSP to be an appropriate natural source of protein and dietary fiber in gluten‐free muffin production.

Review on medicinal Use of Pumpkin Seed on Human Health

The pumpkin belongs to the family of Cucurbitaceae, is a well-known edible plant that has been frequently used as functional food or herbal medicine. Pumpkins contain rich unsaturated fatty acids, phytoestrogens and vitamins E in their seeds that have potential pharmaceutical, nutraceutical, and cosmeceutical properties. Information regarding their nutritional components and therapeutic properties of pumpkin seeds has expanded dynamically in the recent years and this review focus on the three main components of pumpkin seeds that described before. Several types of unsaturated fatty acids are the dominant component in pumpkin seeds which can play a role in the disease prevention and promote health. Pumpkin seeds also contain the important phytoestrogen compounds, i.e., secoisolariciresinol and lariciresinol that have estrogenic-like effect such as preventing hyperlipidemia and osteoporosis for menopausal women. Phytoestrogens in pumpkin seeds also could be related to a reduced hormone-dependent tumor. Pumpkin seeds are rich in vitamin E contents as an emerging free radical scavenger, anti-aging and antioxidant such as a-tocopherol and g-tocopherol. Findings of these studies prove that patents field for the innovation product of pumpkin seeds holds promise for the future along with their immense nutraceutical properties.

NMR and GPC Analysis of Alkyd Resins: Influence of Synthesis Method, Vegetable Oil and Polyol Content.

Alkyd resins are oil-based polymers that have been widely used for generations in the surface coating industry and beyond. Characterization of these resins is of high importance to understand the influence of its components on its behavior, compatibility with other resins, and final quality to ensure high durability. Here, NMR spectroscopy and GPC were used for characterizing differences in the chemical structure, molecular distribution, and dispersity between oil-based and fatty acid-based alkyd polymers made from sacha inchi and linseed oils. Sancha inchi (Plukentia volubilis L.) is a fruit-bearing plant native to South America and the Caribbean, and has a rich unsaturated fatty acid content. The effect of vegetable oil and polyol selection on the synthesis of alkyd resins for coating applications was analyzed. The influence of two different synthesis methods, monoglyceride and fatty acid processes, was also compared. Important structural differences were observed using NMR: one-dimensional spectra revealed the degree of unsaturated fatty acid chains along the polyester backbone, whereas, 2D NMR experiments facilitated chemical shift assignments of all signals. GPC analysis suggested that alkyd resins with homogeneous and high molecular weights can be obtained with the fatty acid process, and that resins containing pentaerythritol may have uniform chain lengths.

Vitamin E dosage variations as antioxidants for improving the quality of fish oil derived from processing waste of Pangasius catfish

The limited availability of tubifex poses a challenge in obtaining green catfish fry. Fish oil, comprising saturated and unsaturated fatty acids, serves as an alternative fat source in commercial feed. Utilizing by-products from catfish processing, fish oil production has garnered attention; however, its rich unsaturated fatty acid content renders it prone to oxidation. Vitamin E emerges as a potential antioxidant to curb this oxidation. This study aims to evaluate fish oil quality with vitamin E supplementation. Peroxide number (PV) and storage duration (0, 3, 6, 9, 12, and 15 days) were analyzed. Fish oil received varying doses of vitamin E (0, 1, 3, 5, and 7 mg/100 g), with each treatment replicated twice. Findings indicated that without vitamin E (0 mg/100 g), PV reached 10.0 meq/kg after 15 days of storage. Conversely, with vitamin E (1, 3, 5, and 7 mg/100 g), PV values after 15 days were 7.2, 6.0, 5.4, and 4.0 meq/kg respectively. The study establishes that higher vitamin E doses effectively prolong fish oil shelf life while conforming to the Codex PV standard (≤ 5 meq/kg).

Identification of Adulterated Evening Primrose Oil Based on GC‐MS and FT‐IR Combined with Chemometrics

AbstractEvening primrose oil has a high market value due to its rich unsaturated fatty acids, so it is likely to be adulterated under the drive of economic interests. In this study, evening primrose oil's fatty acid and physicochemical properties are systematically determined, and the characteristic fatty acids are screened out. The feasibilities of GC‐MS and FT‐IR in identifying oil adulteration are also evaluated. Evening primrose oil's major fatty acids are linoleic acid (C18:2), γ‐linolenic acid (C18:3n6), palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (C18:0). In addition, palmitoleic acid (C16:1), hexadecadienoic acid (C16:2), nonadecanoic acid (C19:0), eicosatrienoic acid (C20:3), erucic acid (C22:1), tricosanoic acid (C23:0) and lignoceric acid (C24:0) are rarely detected in other related studies on the evening primrose oil. This study uses fatty acids as indicators, hierarchical cluster analysis, and cosine similarity analysis to identify the evening primrose oil mixed with >5% peanut oil and >10% sunflower oil. Besides, the principal component analysis also distinguishes evening primrose oil blended with different proportions of peanut and sunflower oils. In summary, this study confirms that fatty acids can be characteristic indexes to identify adulterated evening primrose oil by GC‐MS and FT‐IR combined with chemometrics.Practical Applications: This study further clarifies the major and characteristic fatty acids of evening primrose oil. On this basis, the adulteration of evening primrose oil (taking peanut oil and sunflower oil as an example) is identified. Therefore, it is helpful to assess the quality and identify the authenticity of the evening primrose oil, which is vital for the stability of the evening primrose oil market and the interests of consumers. These methods combined with chemometrics can also be extended to ensure the certification of other oils and to classify oils into different classes. The improvements in vegetable oil quality will also benefit the vegetable oil industry and control bodies.

Effects of natural antioxidants on the oxidative stability of Eucommia ulmoides seed oil: Experimental and molecular simulation investigations

Eucommia ulmoides seed oil with high health potential is prone to oxidative rancidity due to its rich unsaturated fatty acids. In this work, three natural antioxidants were selected for exploring the oxidation resistance of the oil compared with the common synthetic antioxidant BHT. Antioxidant activity and its dispersion and migration as well as oxygen barrier performance were predicted via the bond dissociation enthalpy (BDE), mean square displacement (MSD), binding energy (Ebinding) and permeability coefficient (S). The predicted comprehensive performance is as follows: myricetin > epicatechin > caffeic acid > BHT. Free radical scavenging assay and Rancimat assay confirmed the antioxidant activity and protective effect on oil. That is the protective effect of three natural antioxidants on Eucommia ulmoides seed oil is better than BHT and myricetin shows the optimal comprehensive performance. The induction period of myricetin/lipid system increased 164.5% compared with the control. The experimental results are in good consistent with the simulation predictions.

Proteomic and lipidomics analyses of high fatty acid AhDGAT3 transgenic soybean reveals the key lipase gene associated with the lipid internal mechanism.

Vegetable oil is one of the most important components of human nutrition. Soybean (Glycine max) is an important oil crop worldwide and contains rich unsaturated fatty acids. Diacylglycerol acyltransferase (DGAT) is a key rate-limiting enzyme in the Kennedy pathway from diacylglycerol (DAG) to triacylglycerol (TAG). In this study, we conducted further research using T3 AhDGAT3 transgenic soybean. A high-performance gas chromatography flame ionization detector showed that oleic acid (18:1) content and total fatty acid content of transgenic soybean were significantly higher than those of the wild type (WT). However, linoleic acid (18:2) was much lower than that in the WT. For further mechanistic studies, 20 differentially expressed proteins (DEPs) and 119 differentially expressed metabolites (DEMs) were identified between WT (JACK) and AhDGAT3 transgenic soybean mature seeds using proteomic and lipidomics analyses. Combined proteomic and lipidomics analyses showed that the upregulation of the key DEP (lipase GDSL domain-containing protein) in lipid transport and metabolic process induced an increase in the total fatty acid and 18:1 composition, but a decrease in the 18:2 composition of fatty acids. Our study provides new insights into the deep study of molecular mechanism underlying the enhancement of fatty acids in transgenic soybeans, especially oleic acid and total fatty acid, which are enhanced by over-expression of AhDGAT3.

Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.

Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of Scenedesmus sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in Scenedesmus sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that Scenedesmus sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries.

Major Fatty Acid Profiles and Bioactivity of Seed Oils from Ten Tree Peony Cultivars as a Potential Raw Material Source for the Cosmetics and Healthy Products

As a new woody oleaginous crop, tree peony is now being widely developed and utilized, which attributed to the outstanding oil-use features regarding the fatty acid profiles and bioactivity. The major fatty acid profiles and bioactivity of seed oils in ten tree peony varieties collected from a common garden were investigated in the present study. The results showed that the oil yields, fatty acid profiles, bioactivity of seed oils existed significant variations among ten tree peony varieties (P<0.05). Considered the application value, 'Fengdan' (FD) and 'Ziban' (ZB) was the optimal resources as the high oil yields, rich unsaturated fatty acids especially high α-linolenic acid of 40.46 %, great antioxidant activity with low IC50 values, high ABTS and FRAP values, and strong antimicrobial activity with high DIZ and low MIC/MBC values. The study also confirmed seed oil of tree peony as the potential raw materials sources in functional food, pharmaceuticals and cosmetics for human health.

Performance and digestive characteristics in dairy cattle maintained under grazing regime and supplemented with linseed

Linseed is characterized by the rich unsaturated fatty acids and slow release of oil in the rumen. The objective of the present study was to evaluate the intake, rumen degradability, and digestibility of dairy cattle maintained under grazing regime and supplemented with linseed. Twelve Girolando (¾ Holstein × ¼ Gir) cows with a mean age of 5.2 ± 1.9 years and body weight of 583.9 ± 67.6 kg were used. The cows were maintained in a rotational grazing system on the mombaça grass (Panicum maximum) and divided into 2 experimental groups. The CONTR (n = 6) group received only the base diet (pasture) and the LINS group (n = 6) received pasture plus linseed. Initially, a fifteen-day adaptation period was established to stabilize the desired consumption amount. After this period, the animals received 800 g of linseed for 126 d. The ruminal degradability was evaluated using 2 males with a cannula in the rumen, and they were provided the same experimental diet. The measured dry matter production of pasture did not show differences during the experimental period. The intake of crude protein and ethereal extract was higher, whereas neutral detergent fiber content was lower in the cows supplemented with linseed. However, this did not alter the weight gain and digestibility coefficients, except the digestibility coefficient of the ethereal extract. At the ruminal level, linseed showed low effective and potential degradability with negative effect on grass degradability.

A Review: The Emerging Nutraceutical Potential of Pumpkin Seeds

The pumpkin, belongs to the family of Cucurbitaceae, is a well-known edible plant that has been frequently used as functional food or herbal medicine. Pumpkins contain rich unsaturated fatty acids, phytoestrogens and vitamins E in their seeds that have potential pharmaceutical, nutraceutical, and cosmeceutical properties. Information regarding their nutritional components and therapeutic properties of pumpkin seeds has expanded dynamically in the recent years and this review focus on the three main components of pumpkin seeds that described before. Several types of unsaturated fatty acids are the dominant composition in pumpkin seeds which can play a role in the disease prevention and promote health. Pumpkin seeds also contain the important phytoestrogen compounds, i.e., secoisolariciresinol and lariciresinol that have estrogenic-like effect such as preventing hyperlipidemia and osteoporosis for menopausal women. Phytoestrogens in pumpkin seeds also could be related to a reduced hormone-dependent tumor. Pumpkin seeds are rich in vitamin E contents as an emerging free radical scavenger, antiaging and antioxidant such as a-tocopherol and g-tocopherol. Findings of these studies prove that patents field for the innovation product of pumpkin seeds holds promise for the future along with their immense nutraceutical properties.

Determination of Metabolizable Energy of Rich Unsaturated Fatty Acids Dry-Fat in Chicken Diets Using Chemical, Biological and Mathematical Methods

Two biological experiments were conducted to determine apparent Metabolizable Energy (AME) of rich unsaturated fatty acids dry-fat (Polyfat as an example, PF) using corn or corn-soybean meal diets with ® different inclusion levels of PF in adult cockerel diets. The biological experiments designed to follo w procedures of excreta total collection method (TCM). The chemical evaluation of PF included determination of peroxide No., acid No., fatty acid profile and gross energy. Then different mathematical equations were applied to calculate AME of PF based on the chemical evaluation. The determined values of AME of PF using chemical methods were 7160 and 7188 kcal/kg for PF. Among biological experiments, using restricted quantity of corn diet with high levels of PF (25 or 50% of diet) resulted in lower AME values (4724 and 3992kcal/kg PF). While applying ad libitium consumption of corn-soybean practical diet containing 3% soybean oil (as reference oil), 3.8% of PF or 50:50 mixture of both (1.5% SO+1.9% PF), gave more realistic value of 6973 kcal/kg for PF. Mixing PF with soybean oil showed clear synergism effect and added caloric value of 9.6% (737 kcal) to the mixture above the expected value. Applying simple regression procedures on results of chemical and biological evaluations showed highly significant (p<0.001) relationships between AME of PF and either digestible fat, dietary saturated fatty acids (%) or supplemental stearic acid (%) in diet. These relationships have been presented as high confidant prediction equations with r values ranged 2