Sheath blight of rice caused byRhizoctonia solaniis regarded as one of the most widely distributed diseases of rice, and is one of the major production constraints for rice in India and most rice-growing countries of Asia. Biological control of plant diseases using antagonistic bacteria is now considered as a promising alternative to the use of hazardous chemical fungicides or bactericides. Several bacterial endophytes have been reported to support growth and improve the health of the plants and therefore, may be important as biocontrol agents.In the present study, putative antifungal metabolites were extracted from rice foliage endophyteLysinibacillus sphaericusKJ872548 by solvent extraction methods and purified using HPTLC techniques. Separated bands were subjected to assess thein vitroantagonistic activity toward rice sheath blight pathogenRhizoctonia solaniusing a dual culture method. Partially purified active fraction B2 obtained from HPTLC analysis showed the highest percentage of inhibition (76.9%). GC MS and FTIR analyses of B2 revealed the compound as1, 2-Benzenedicarboxylic acid butyl 2-Ethylhexyl ester, a strong antifungal volatile organic compound. Light microscopic analysis of the fungal mycelium from the dual culture plate of both culture filtrate and 1, 2-Benzenedicarboxylic acid butyl 2-Ethylhexyl ester disclosed strong mycolytic activity as evident by mycelial distractions and shrinkage. This is the first report on antifungal production by endophyticLysinibacillus sphaericusagainstR. solani,the rice sheath blight pathogen. The findings of this study biologically prospect the endophyteL. sphaericusas an inexpensive broad spectrum bioagent for eco-friendly, economic and sustainable agriculture.