Rice Fields In Asia Research Articles

Chromosome-level genome assembly from a single planthopper Nilaparvata muiri (Hemiptera: Delphacidae)

The planthopper Nilaparvata muiri is a sister species to N. lugens (Hemiptera: Delphacidae), a notorious insect pest in Asian rice fields. N. muiri and N. lugens have a different host preference despite the similarities in many biological features. To better understand the adaptive evolution of planthoppers, comprehensive genomic information on N. muiri and N. lugens are urgently needed. In this study, we used ultra-low input PacBio HiFi libraries and Hi-C sequencing technologies to assemble a reference genome of a single N. muiri at the chromosomal level. The genome size was determined to be 531.62 Mb with a contig N50 size of 2.47 Mb and scaffold N50 size of 38.37 Mb. Totally, 96.61% assembled sequences were anchored to the 15 pseudo-chromosomes. BUSCO analysis yielded an Insecta completeness score of 98.6%. A total of 22,057 protein-coding genes were annotated, and 168.16 Mb repetitive sequences occupying 31.63% of genome were pinpointed. The assembled genome is valuable for evolutionary and genetic studies of planthoppers, and may provide sights to pest control.

Impacts of rainfall and rainfall anomalies on the population dynamics of rodents in southeast Asian rice fields.

The mechanisms that regulate multi-annual population dynamics of rodent pest species of cereal crops is often unknown. Better knowledge of such aspects can aid pest management and in turn improve food security and human health. The patterns and processes of the population dynamics of Rattus argentiventer, in rice fields of Indonesia, and Rattus tanezumi, in rice fields of the Philippines were assessed in this article. The meta-analysis of trapping data over 20 years in Indonesia, and 16 years in the Philippines indicated that rodent populations in rice fields did not show a regular multi-annual pattern. Rattus argentiventer populations in Indonesia responded to less rainfall from the current year. Rattus tanezumi populations in the Philippines responded positively to both rainfall and rainfall anomaly with a 1-year time lag. Our study of long-term population data indicates that certain combinations of rainfall parameters could be useful to predict years when there is higher rodent abundance in rice fields. The key rodent pest species in rice fields in Indonesia (R. argentiventer) and the Philippines (R. tanezumi) differ, and the populations of each species respond differently to rainfall anomalies. Other factors such as crop cover and water availability may also be important and should be considered in future work. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Mitigating atmospheric methane emissions from Asian rice fields: a review of potential and promising technical options

Agriculture serves as a significant anthropogenic source of methane emissions. Numerous recent studies have examined the factors influencing methane emissions and have developed emission models. However, there is no a bridging review study related to methene emissions in Asia as one of the primary methane emitters. This review is divided into two manuscripts. In this first manuscript, I explore the process of methane emission and the factors that impact methane production and emissions. Meanwhile, the present state of studies conducted in various Asian countries and knowledge gaps are elaborated in the second manuscript. I elaborate several factors that influence methane production and their roles in the emission process. Further, I highlight that the gas is mostly produced in zero oxygen condition, although, a little concentration of methane also can be generated in oxic condition. This finding provides basic knowledge that contribute to the future research on methane emissions in rice field ecosystems. Eventually, I also explore various recommended technical solutions to reduce the gas emission.

Development and demographic parameters of Fall Armyworm (Spodoptera frugiperda J.E. Smith) when feeding on rice (Oryza sativa)

Fall Armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), native to the Americas, is a polyphagous insect pest feeding on more than 350 plant species. We studied the developmental and demographic parameters of the maize (Zea mays) strain of FAW on rice (Oryza sativa), and compared the results with its prime host, maize. The developmental period from egg to adult among rice varieties did not differ significantly; however, it did differ significantly between rice and maize, as feeding on rice rather than maize extends development duration of FAW larvae by 15.15%. FAW larvae collected and reared on maize were found to be of significantly higher weight than those reared on rice at two sequential dates of their development; pupal weight however was observed as statistically similar between these two host crops. Regardless of the host, female adults always emerged before males; in maize, female FAW appeared 3.36 days earlier than males. Females derived from rice had longer pre-oviposition periods and shorter oviposition ones than those derived from maize. In rice and maize, the age-specific fecundity rate (mx) peaked at 40 days and 33 days, respectively. When the Fall Armyworm consumed maize instead of rice, there was an increase in the reproduction rate (R0), the intrinsic rate of natural increase (rm), and the finite rate of increase (λ). For instance, when FAW fed on rice, the rm value was 0.121, whereas it rose to 0.173 when FAW fed on maize. Feeding on rice instead of maize resulted in significantly longer mean length of generation (tG) and doubling time (tD) for the fall armyworm (FAW). This suggests that it took a longer time for the FAW population to double when it was fed rice under controlled greenhouse conditions. In summary, our research suggests that FAW can survive and complete its life cycle on rice plants and on multiple varieties of rice in Bangladesh. However, field verification is necessary before drawing strong conclusions as to the risk posed by FAW in rice. This requires additional studies of FAW and associated insect community dynamics under non-controlled conditions and in the context of multi-species interactions in Asian rice fields.

Characterization and functional analysis of an α-adrenergic-like octopamine receptor in the small brown planthopper Laodelphax striatellus

As an important biogenic amine in invertebrates and corresponding to the neurotransmitter norepinephrine in vertebrates, octopamine (OA) regulates diverse physiological and behavioral processes by binding to specific octopamine receptors (OARs) in invertebrates. At present, OARs have been identified and characterized in several insects. However, less is known about the OARs of Laodelphax striatellus, one of the most destructive pests in East Asian rice fields. In the present study, an α1-adrenergic-like OAR (LsOA1) from L. striatellus was cloned. LsOA1 has the typical characteristics of G-protein coupled receptors and is clustered with other insect homologs. The transcript level of LsOA1 varied in various stages and tissues, and was highly expressed at the egg stage and in the brain. Silencing of LsOA1 causes a reduction in vitellogenin (LsVg) and vitellogenin receptor (LsVgR) expression. Although LsOA1 interference did not affect the fecundity and survival of L. striatellus, the hatching rate of L. striatellus was significantly reduced, and the hatching period was prolonged. The decrease in the amount of honeydew excreted after silencing LsOA1 indicates that LsOA1 may be involved in regulating the feeding behavior of L. striatellus. In addition, the interference of LsOA1 significantly reduced the expression of capsid protein (CP) and viral RNA3 segment (RNA3) in rice stripe virus (RSV)-viruliferous L. striatellus, but did not affect the vertical transmission rate of RSV. The present study demonstrated that LsOA1 played a crucial role in the physiological and behavioral processes of L. striatellus, which will provide the basis for developing a new target gene for pest control.

Meloidogyne graminicola-A Threat to Rice Production: Review Update on Distribution, Biology, Identification, and Management.

Simple SummaryNew risks to plant health are constantly emerging. Such is the case of the rice root knot nematode Meloidogyne graminicola, adapted to flooded conditions and representing a risk to all types of rice agro-systems. It has been recently detected in Italy and added to the European and Mediterranean Plant Protection Organization (EPPO) Alert List. The presence of this nematode in Europe poses a threat to rice production, as there is a high probability to spread, due to trade activities and climate changes. In view of its importance, an extensive updated review was carried out. Rice (Oryza sativa L.) is one of the main cultivated crops worldwide and represents a staple food for more than half of the world population. Root-knot nematodes (RKNs), Meloidogyne spp., and particularly M. graminicola, are serious pests of rice, being, probably, the most economically important plant-parasitic nematode in this crop. M. graminicola is an obligate sedentary endoparasite adapted to flooded conditions. Until recently, M. graminicola was present mainly in irrigated rice fields in Asia, parts of the Americas, and South Africa. However, in July 2016, it was found in northern Italy in the Piedmont region and in May 2018 in the Lombardy region in the province of Pavia. Following the first detection in the EPPO region, this pest was included in the EPPO Alert List as its wide host range and ability to survive during long periods in environments with low oxygen content, represent a threat for rice production in the European Union. Considering the impact of this nematode on agriculture, a literature review focusing on M. graminicola distribution, biology, identification, and management was conducted.

A Comparative Analyses of the Complete Mitochondrial Genomes of Fungal Endosymbionts in Sogatella furcifera, White-Backed Planthoppers.

Sogatella furcifera Horvath, commonly known as the white-backed planthoppers (WBPH), is an important pest in East Asian rice fields. Fungal endosymbiosis is widespread among planthoppers in the infraorder Fulgoromorpha and suborder Auchenorrhyncha. We successfully obtained complete mitogenome of five WBPH fungal endosymbionts, belonging to the Ophiocordycipitaceae family, from next-generation sequencing (NGS) reads obtained from S. furcifera samples. These five mitogenomes range in length from 55,390 bp to 55,406 bp, which is shorter than the mitogenome of the fungal endosymbiont found in Ricania speculum, black planthoppers. Twenty-eight protein-coding genes (PCGs), 12 tRNAs, and 2 rRNAs were found in the mitogenomes. Two single-nucleotide polymorphisms, two insertions, and three deletions were identified among the five mitogenomes, which were fewer in number than those of four species of Ophiocordycipitaceae, Ophiocordyceps sinensis, Hirsutella thompsonii, Hirsutella rhossiliensis, and Tolypocladium inflatum. Noticeably short lengths (up to 18 bp) of simple sequence repeats were identified in the five WBPH fungal endosymbiont mitogenomes. Phylogenetic analysis based on conserved PCGs across 25 Ophiocordycipitaceae mitogenomes revealed that the five mitogenomes were clustered with that of R. speculum, forming an independent clade. In addition to providing the full mitogenome sequences, obtaining complete mitogenomes of WBPH endosymbionts can provide insights into their phylogenetic positions without needing to isolate the mtDNA from the host. This advantage is of value to future studies involving fungal endosymbiont mitogenomes.

Optimal design and setting of rotary strip-tiller blades to intensify dry season cropping in Asian wet clay soil conditions

Fine-textured clayey soils dominate Asian rice fields that are kept either fallow or cultivated with non-rice crops after harvest of monsoon rice. Use of seeding machinery compatible with the principles of conservation agriculture on such soils, however, has not been promising. Under these conditions – which predominate the population and poverty dense areas of coastal South Asia – such machinery fails to open a furrow or throws excessive soil out of the tilled furrow during strip-till seeding. This results in a poor seed coverage at planting jeopardizing crop establishment. In response, this soil bin study investigated strip-tillage blade designs and settings to optimize rotary strip-till system for wet clay soil conditions common in South Asian rice fields. Three designs of C type rotary blade (conventional, medium and straight) and two blade settings (four and six blades per row; 50 and 100 mm cutting widths) were tested at three blade operating depths (50, 75, and 100 mm) using a tillage test rig and a soil bin, and a high-speed camera to understand the processes of soil cutting, throwing, backfilling, and creation of furrow seedbed. The soil bin soil consisted of a wet sandy-clay-loam soil with a moisture content of 28.2% (85% of field capacity) and was compacted to the bulk density of 1440 kg m−3. Using the test rig, rotary speed of the blades was maintained at 480 rpm and forward speed at 0.4 m s−1. At four blades per row setting, all blades created high amounts of optimum clods (1–20 mm size). The conventional and medium blades threw too much soil out of the strip-tilled furrow while the straight blade created adequate backfill at 75 and 100 mm operating depths. At 6 blades per row setting, all blades produced high amounts of backfill at any depths, but the straight blade also produced the highest amounts of optimum clods and a uniform furrow. Considering machine and energy costs, blade performance, and the necessity of minimizing soil disturbance in strip-tillage, our study indicates that the use of straight blades (four blades per row) operated at a depth of 75 or 100 mm are more ideal. These specifications are likely to enhance strip-tillage stand establishment in fine-textured soils with high moisture contents, though further work is needed under actual field conditions to confirm suitability of the proposed strip-till system for crop establishment in currently fallowed as well as the intensively cropped lands of Asia.

Taxonomic assessments of some Cyprinotinae Bronstein, 1947 species(Crustacea: Ostracoda) from Japanese and Korean rice fields, including(re-) descriptions of six species and a review of the type species of the subfamily.

Rice is the staple food crop for approximately half the world's population, but some common and abundant taxa inhabiting rice fields, such as ostracods, are poorly studied. For many ostracod species only brief, initial descriptions are available, hindering reliable identifications, and so their ecological roles in rice fields remain obscure. This study partially addresses this problem by assessing the taxonomic validity of six species of Cyprinotinae ostracods reported from rice fields in Japan and South Korea: Cyprinotus uenoi Brehm, 1936, Hemicypris megalops Sars, 1903, Hemicypris ovata Sars, 1903, Hemicypris vulgaris Okubo, 1990, Heterocypris rotundata (Bronstein, 1928), and Hemicypris kawagaensis Okubo, 2004 nomen nudum. Type material of C. uenoi was not designated and Brehm's ostracod collection is missing, but Japanese and Korean records of C. uenoi differ significantly from Brehm's original description. The Japanese and Korean species is described and named herein as Cyprinotus cassidula sp. nov. Japanese specimens reported to be Hemicypris megalops are probably misidentified, but details of the carapace of Hemicypris megalops are obscure due to poor preservation of type material. Examination of topotype material of Hemicypris posterotruncata Bate, 1970, a sub-fossil from near Lake Turkana in Kenya, demonstrates that this species is extant in Asian rice fields. Investigation of type material of Hemicypris kliei (Lindroth, 1953) has revealed that it is not a senior synonym of Hemicypris posterotruncata, contrary to previous studies. Size and morphological data indicates that Hemicypris vulgaris is a large form of Hemicypris posterotruncata and the two species are treated as synonyms herein. Examination of paralectotypes of Hemicypris ovata indicates that it is very similar to Hemicypris posterotruncata, especially the larger morphotypes, but as these two species can be distinguished by morphological features they are both retained as separate species. However, Japanese records of Hemicypris ovata are considered misidentifications of the larger forms of Hemicypris posterotruncata. Hemicypris kawagaensis, which has only been reported once from East Asia, is herein regarded as a junior synonym of Hemicypris barbadensis Broodbakker, 1983, and is likely an alien species in Asia. Asian specimens previously identified as Heterocypris rotundata show sufficient differences to European specimens to determine that they are not conspecific. This species is described as Heterocypris savatenalintonae sp. nov. Taxonomic descriptions of Hemicypris ovata, Hemicypris posterotruncata, Hemicypris megalops, Hemicypris kliei, Heterocypris savatenalintonae sp. nov., and Cyprinotus cassidula sp. nov. are presented. Finally, it is noted that past redescriptions of Cyprinotus cingalensis Brady, 1886, the type species of the genus and the subfamily Cyprinotinae Bronstein 1947, are based partly on other species.

Ilyocypris (Crustacea: Ostracoda) species in North East Asian rice fields; description of one new species, and redescriptions of Ilyocypris dentifera Sars, 1903 and Ilyocypris japonica Okubo, 1990.

Ilyocypris species are abundant and widespread in North East Asian rice fields, but for many species their taxonomy is poorly defined, which negatively impacts ecological studies on rice field ecosystems. Herein, two of these species, Ilyocypris dentifera Sars, 1903 and Ilyocypris japonica Okubo, 1990, are redescribed, and a third species, Ilyocypris incus sp. nov., is described and named. This latter species has previously been reported from Japan and possibly Korea under the names Ilyocypris angulata Sars, 1903 and I. dentifera, but study of the hemipenes demonstrates that it is a separate species. Ilyocypris nipponica Okubo, 2004 nomen nudum previously reported from Japan is herein considered to be a junior synonym of I. dentifera. Tuberculated and non-tuberculated forms of both I. dentifera and I. japonica were recovered during this study and the presence or absence of tubercles is postulated to be related to environmental conditions, such as water chemistry or predation pressures. Although tuberculated forms of I. dentifera do occur, we concur with some previous studies that Ilyocypris angulata Sars, 1903 should be treated as a separate species, based on features of the hemipenes and carapaces. Figures of the carapaces and hemipenes of I. angulata, and Ilyocypris mongolica Martens, 1991, which is morphologically very close to I. japonica, are also presented for comparisons. This study confirms that three species of Ilyocypris inhabit North East Asian rice fields: I. dentifera, I. japonica and Ilyocypris incus sp. nov. Extant Ilyocypris species in North East Asia, including those from habitats other than rice fields, can be divided into two groups based on the Zenker organ, hemipenis, and sixth limb morphologies. These groups are postulated to represent two distinct lineages of Ilyocypris in North East Asia.

Vertical stratification in foraging activity of Chaerephon plicatus (Molossidae, Chiroptera) in Central Thailand

Chaerephon plicatus consumes large amounts of planthoppers, which are the most serious pest in Asian rice fields. Brown planthoppers (Nilaparvata lugens) are known to migrate at high altitude and can cause severe damage and economic loss over a large area. However, we lack evidence whether this bat species also forage at high altitudes. This study investigated the stratification in foraging activity of C. plicatus at approximately 0, 100, and 200 m above ground level by using a helium-filled balloon-kite. The activity of C. plicatus aloft (100 m and 200 m) on average was six to twelve times higher than that close to the ground. Peak activity corresponded with the top of the nocturnal stable boundary layer, which is also the layer of maximum temperature and wind speed. Nilaparvata lugens has been found flying at the altitude where peak bat foraging activity occurs, which suggests that C. plicatus actively follows these migratory insects in the air. Thus, C. plicatus bats potentially play an important role in insect pest suppression in the areas they inhabit as well as at the landscape scale. However, the height of peak activity also corresponds with the height of wind turbine blades, thus, such turbines may pose a serious threat to the species.

Analysis of Homologs of Cry-toxin Receptor-Related Proteins in the Midgut of a Non-Bt Target, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae).

The brown planthopper (BPH) Nilaparvata lugens is one of the most destructive insect pests in the rice fields of Asia. Like other hemipteran insects, BPH is not susceptible to Cry toxins of Bacillus thuringiensis (Bt) or transgenic rice carrying Bt cry genes. Lack of Cry receptors in the midgut is one of the main reasons that BPH is not susceptible to the Cry toxins. The main Cry-binding proteins (CBPs) of the susceptible insects are cadherin, aminopeptidase N (APN), and alkaline phosphatase (ALP). In this study, we analyzed and validated de novo assembled transcripts from transcriptome sequencing data of BPH to identify and characterize homologs of cadherin, APN, and ALP. We then compared the cadherin-, APN-, and ALP-like proteins of BPH to previously reported CBPs to identify their homologs in BPH. The sequence analysis revealed that at least one cadherin, one APN, and two ALPs of BPH contained homologous functional domains identified from the Cry-binding cadherin, APN, and ALP, respectively. Quantitative real-time polymerase chain reaction used to verify the expression level of each putative Cry receptor homolog in the BPH midgut indicated that the CBPs homologous APN and ALP were expressed at high or medium-high levels while the cadherin was expressed at a low level. These results suggest that homologs of CBPs exist in the midgut of BPH. However, differences in key motifs of CBPs, which are functional in interacting with Cry toxins, may be responsible for insusceptibility of BPH to Cry toxins.

Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2

Bacterial leaf blight of rice (BLB), caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent. IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC3F3 lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines (BC3F3 5.1.5.1, BC3F3 5.1.5.12, BC3F3 8.5.6.44, BC3F3 9.5.4.1 and BC3F3 9.5.4.23) were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security.

Paddy Field Mapping Using Topographic and Scattering Features of PolSAR Data

Irrigated rice fields in Asia have a distinct topography and scattering mechanisms. Because of these factors, many studies using radar backscattering information have been conducted to improve the mapping accuracy; however, relatively little attention has been paid to the topographic features (TF) provided by polarimetric synthetic aperture Radar (PolSAR) data. To address this issue, this letter presents a simple rice field mapping method in its late-vegetative stage using both the TF and scattering features (SF) of the PolSAR data. First, two TF, the polarization orientation angle and the dominant beta angle from the PolSAR data, were analyzed with respect to the slope from a digital elevation model. The results indicated that this feature pair had a moderately high correlation. As a result, flat areas were easily extracted using these two TF. Second, to use the SF of rice in the late-vegetative stage, the copolarized ratio and the copolarized phase difference were simply combined to map only paddy fields, of which scattering was dominated by the double-bounce scattering caused by the ground and canopy reflection. The results using C-band Radarsat-2 data were compared with a support vector machine classifier and demonstrated that the proposed mapping method provided an overall better performance. The proposed method might also be efficiently applied, with modification, for the aforementioned purpose in inaccessible areas.

Deployment and Performance of a Uav for Crop Spraying

Small, unmanned aircraft systems (UAS) provide an opportunity for pesticide spray application in which the applicator can be displaced from close proximity of the spray discharge and in which the spray application can be made with highly targeted spatial resolution, particularly in challenging geographic terrain. In this project, a commercially manufactured UAS-mounted spray system was deployed in high-value specialty crops in California. The UAS used in this project was a unmanned aerial vehicle (UAV) and an associated ground control station that provided a means for remote piloting of the aircraft. The aircraft was a petrol-powered helicopter (RMAX, Yamaha Motor Co. USA, Cypress, CA USA) originally developed for spraying of rice fields in Asia. In this test, the primary experimental areas for spray deposition and performance assessment included a 0.61 ha block of Cabernet Sauvignon wine grapes located at the University of California Oakville Field Station in Napa County, CA USA. The block consisted of 42 rows, each 61 m long with a row spacing of2.4 m. Depending on the spray method deployed, specifically, the swath width used and the flight pattern flown, the UAS spray application could achieve 2.0 to 4.5 ha/h work rates while applying volume rates of 14.0 to 39.0 L/ha. Spray deposition on the grape foliage increased with applied volume rate. In comparisons to ground-based sprays at 935 L/hr, deposition in the grape canopy from the UAS at 47 L/ha was similar.

Batch adsorption studies on the removal of malachite green from water by chemically modified Azolla pinnata

Azolla pinnata (AP), a common water fern found in rice fields in Asia and cultivated for biological nitrogen fixation, was studied as a potential adsorbent for the removal of malachite green in a batch adsorption system. The batch adsorption studies, involving unmodified AP (UAP) and two chemically modified AP—H3PO4-treated AP (PAP) and NaOH-treated AP (NAP)—included the effects of adsorbent dosage, pH, ionic strength, contact time, thermodynamics and kinetics studies, estimation of activation energy and regeneration experiments. Three isotherm models namely the Langmuir, Freundlich and Dubinin–Radushkevich models were used and the Langmuir model best represented all the three adsorption systems with maximum adsorption capacity (qm) of UAP, PAP and NAP at 25°C, to be at 87.0, 292.1 and 109.6 mg g−1, respectively. The kinetics modelling included the pseudo-first-order, pseudo-second-order, Weber–Morris intraparticle diffusion and the Boyd models. Thermodynamic studies showed that all the three adsorption systems are endothermic and spontaneous in nature. All three adsorbents were regenerated with 0.1 mol L−1 NaOH and were effective even after five cycles.

Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52.

The brown planthopper (BPH) is the most serious insect pest of rice in Asia. The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (BROWN PLANTHOPPER RESISTANCE 26) and BPH25. Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site–leucine-rich repeat (CC–NBS–LRR) protein. BPH26 mediated sucking inhibition in the phloem sieve element. BPH26 was identical to BPH2 on the basis of DNA sequence analysis and feeding ability of the BPH2-virulent biotype of BPH. BPH2 was widely incorporated in elite rice cultivars and was well-cultivated in many Asian countries as a favorable gene resource in rice breeding against BPH. However, BPH2 was rendered ineffective by a virulent biotype of BPH in rice fields in Asia. In this study, we suggest that BPH2 can be reused by combining with other BPH resistance genes, such as BPH25, to ensure durable resistance to BPH.

Vertisol prevent cadmium accumulation in rice: Analysis by ecophysiological toxicity markers

Alfisol and vertisol cover more than fifty percent of rice fields in Asia. Rice cultivated in these soils is found to be contaminated with cadmium (Cd). Influence of physiochemical properties of these soils on Cd accumulation and ecophysiological functions of Oryza sativa L. cv MTU 7029 and MO 16 were analyzed. Plants grown in vertisol accumulated lower amount of Cd compare with those in alfisol. Soil properties such as cation exchange capacity and alkalinity played major role in decrease in Cd accumulation. Difference in Cd accumulation also reflected as more decrease in ecophysiological components such as biomass, relative water content and non-photochemical quenching among plants grown in alfisol. Plants grown in vertisol produced more flavanols, chlorophyll, anthocyanin, sugar, and protein than those in alfisol under Cd stress. But phenolic content was higher in plants grown in alfisol. Quantum efficiency of PSII, thiols, and silicon was increased while carotenes and calcium were decreased upon Cd accumulation. These changes were irrespective of soil type. Among the varieties chosen for the study, MTU 7029 accumulated more Cd compare with MO 16. It was concluded that soil properties caused difference in Cd accumulation among the plants grown in soils chosen and this would differentially reflect on specific ecophysiological markers.

The Complete Mitochondrial Genome of the Stalk-Eyed Bug Chauliops fallax Scott, and the Monophyly of Malcidae (Hemiptera: Heteroptera)

Chauliops fallax Scott, 1874 (Hemiptera: Heteroptera: Malcidae: Chauliopinae) is one of the most destructive insect pests of soybean and rice fields in Asia. Here we sequenced the complete mitochondrial genome of this pest. This genome is 15,739 bp long, with an A+T content of 73.7%, containing 37 typical animal mitochondrial genes and a control region. All genes were arranged in the same order as most of other Heteroptera. A remarkable strand bias was found for all nine protein coding genes (PCGs) encoded by the majority strand were positive AT-skew and negative GC-skew, whereas the reverse were found in the remaining four PCGs encoded by the minority strand and two rRNA genes. The models of secondary structures for the two rRNA genes of sequenced true bugs and Lygaeoidea were predicted. 16S rRNA consisted of six domains (domain III is absent as in other known arthropod mitochondrial genomes) and 45 helices, while three domains and 27 helices for 12S rRNA. The control region consists of five subregions: a microsatellite-like region, a tandem repeats region and other three motifs. The unusual intergenic spacer between tRNA-H and ND4 only found in the species of Lygaeoidea, not in other heteropteran species, may be the synapomorphy of this superfamily. Phylogenetic analyses were carried out based on all the 13 PCGs showed that Chauliopinae was the sister group of Malcinae and the monophyly of Lygaeoidea.

Pollination of chasmogamous flowers and the effects of light and emergence time on chasmogamy and cleistogamy inMonochoria vaginalis

Monochoria vaginalis is one of the most serious weeds of rice fields in Asia. The species has both chasmogamous (CH) and cleistogamous (CL) flowers on individual plants. The objective of this study is to clarify the reproductive characteristics that affect the selfing rate of M. vaginalis. An emasculation experiment revealed that the CH flowers emasculated just after flower opening produced seeds. The seed production in the flowers emasculated just after flower opening was less than that of the flowers emasculated and hand-pollinated with pollen from another plant. These results indicate that, although self-pollination before anthesis occurs, cross-pollination also can produce seeds. An experimental manipulation of light and the emergence time of the plants revealed that more than half of the flowers produced were CL flowers and the CH/CL ratio decreased with reduced light availability. The plants that emerged later produced as many CH flowers as those that emerged earlier. There was no significant difference between the CH and CL flowers in the seed number per fruit and the seed mass. These results suggest that M. vaginalis has reproductive characteristics that make the selfing rate high; however, outcrossing also can occur. Under favorable light intensity conditions, such as on the edges of paddy fields or in fallow fields, M. vaginalis will produce more CH flowers and will have a higher outcrossing rate than in shaded conditions.