This research aims to determine the quality of the substrate of the Wiringtasi River, Barru Regency, Indonesia. The research locations include location 1, namely the location around the rice field area; location 2, namely the location around the pond area and beach; and location 3, namely the location around the river estuary where many mangrove plants grow around it. Subtract samples were taken at the research location, then the samples were taken to the laboratory for analysis. The results of laboratory analysis are made in the form of pictures and tables, then analyzed using descriptive analysis. The results of the research show that in this study the substrate quality of the Wiringtasi River, Barru Regency, Indonesia, each had a pH value of the substrate in the range of 8.05-8.64, total nitrogen was in the range. Nitrogen was in the range of 0.049-0.121%. The organic material content of the substrate is in the range of 0.33-0.59. The ammonia (NH3) content of the substrate is in the range of 0.019–0.45 ppm. The nitrite content of the research location is in the range of 0.0025-0.0064 ppm. The substrate texture of location 1 is dusty clay, while locations 2 and 3 are sandy.