Rhesus Monkey Red Cells Research Articles

Rosette formation between human T-lymphocytes and unsensitized rhesus monkey erythrocytes

Between 60 and 70% of peripheral blood lymphocytes in healthy adults form rosettes with rhesus monkey erythrocytes. Less than 2% of these rosetting lymphocytes carry detectable amounts of surface immunoglobulins. Using thymocytes, peripheral blood lymphocytes from normal adults or from patients with T cell deficiencies, a linear correlation existed between the percentages of rosettes formed with rhesus monkey and with sheep erythrocytes. It is concluded that rosette formation with rhesus monkey red cells is a feature of human T lymphocytes. Rosette inhibition experiments suggest that two different, though similar lymphocyte receptors are responsible for rosette formation with sheep, and with monkey erythrocytes. Both receptors are present in accessible form only on T lymphocytes, but can be demonstrated on B lymphocytes after Vibrio cholerae neuraminidase treatment.

The demonstration of human Gm-like serum -globulin types using nonhuman primate reagents. I. Experiments with anti-gibbon globulin serums.

An antiglobulin serum was prepared by immunizing rabbits with serum globulin of gibbons. This reagent was used for an inhibition test with rhesus monkey red cells coated with rhesus isoantibodies or baboon red cells coated with isoantibodies as indicator. With this test individual differences could be demonstrated in human γ-globulins comparable to the Gm types.

The definition of two I antigen components.

Abstract. The I blood group antigen has two main components named IFetal (IF) and IDeveloped (Id). The IF component is present on all human red cells including those of icord and iadult persons, and also on Rhesus monkey red cells. The ID component develops slowly on the red cells before birth and to a greater extent in the 18 months after birth. Inhibition studies with human milk show that strongly‐inhibitable anti‐I sera are of the anti‐ID variety, but only a minority of such sera are inhibitable. Comparative studies on the red cells of 12 newborn infants suggest that the same sequence of I antigen development occurs in them all, but different infants vary in the amount of I development that they have achieved at the time of birth. I antigen variants affect mainly the ID component, and it is this part of the antigen that may be weakened on the red cells of people with leukemia.

The Definition of Two I Antigen Components

The I blood group antigen has two main components named I^Fetal (I^F) and I^Developed (I^D). The I^F component is present on all human red cells including those of icord and iaduit persons, and also on Rhesus monkey red cells. The ID component develops slowly on the red cells before birth and to a greater extent in the 18 months after birth. Inhibition studies with human milk show that stronglyinhibitable anti-I sera are of the anti-I^D variety, but only a minority of such sera are inhibitable. Comparative studies on the red cells of 12 newborn infants suggest that the same sequence of I antigen development occurs in them all, but different infants vary in the amount of I development that they have achieved at the time of birth. I antigen variants affect mainly the ID component, and it is this part of the antigen that may be weakened on the red cells of people with leukemia.