With the rapid development of smart healthcare, accurate Heart Rate Variability (HRV) estimation for the early detection of diseases has become a hot research topic. Advanced work uses the wireless signal to estimate the heartbeat in a contact-free way, which usually cannot separate multiple users or work in a dynamic environment. In this paper, we propose a lightweight heartbeat-sensing method based on RFID tag pairs, which focuses on HRV extraction in a more general sensing scenario. Based on the tag-pair design, we build a novel heartbeat and respiration model to describe the signal relationship between the two tags from the time and space domains. Based on the model, we propose a Calibrated Temporal-Spatial IQ-Shaping-based signal cancellation algorithm to cancel the respiration and extract the heartbeat. To remove the interference in dynamic measurement, we build an IQ-based signal model via a Principal Component Analysis-based interference estimation. To reduce the statistical error in HRV extraction, we further design a neural network to predict the HRV index. We have implemented a system prototype in a real environment with COTS RFID devices. Extensive experiments show that our system can achieve a median RMSSD error of 7.51ms, which satisfies the medical demand in HRV measurement.