AbstractWidespread adoption of water reuse technologies is critical to the development of a circular economy and to reduce the pressure on increasingly scarce freshwater resources. However, the adoption of water reuse technologies involves a complex and multi-level decision-making process, influenced by different factors that hinder or support such adoption, namely barriers and drivers. The present research provides a first identification of such factors from a value chain perspective. To do so, the paper first conceptualises the water reuse value chain, including the actors and stages required to bring collected wastewater to the reuse destination. It then conducts an exploratory case study in the context of an Italian water utility facing increasing water stress. External barriers such as societal perceptions and lack of demand for recycled water emerge as particularly influential in hindering the adoption of water reuse technologies. Drivers emerge from both the external and internal environment, with the level of water scarcity and the effectiveness of collaboration and coordination between different institutional and policy actors being particularly strong. Barriers and drivers are found to be of varying relevance depending on the characteristics of the facilities and the reuse value chain analysed, including the operational status of the reclamation facility, the type of final reuse, and the roles in the value chains. The paper provides relevant implications for academics, policymakers, and adopters of water reuse technologies for a more circular and environmentally sustainable water sector, offering useful insights for decision-makers in related sectors. Graphical abstract