Retention Time In Digestive Tract Research Articles

Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect.

Liposomes have been broadly used in pharmaceutical field to overcome oral absorption barriers, such as gastric acid, tenacious mucus or intestinal epithelia. However, the concrete in vivo absorption mechanisms of liposomes are still indistinct. This study aims to visually elucidate the effect of particle size and surface characteristics on in vivo translocation of oral liposomes by fluorescence resonance energy transfer (FRET) effect. We fabricated liposomes of various sizes (100nm, 200nm and 500nm) and surface characteristics (anionic, cationic and PEGylated) which are also labeled with FRET probes for discriminating the intact liposomes. We then investigated the in vivo fate of those different liposomes upon oral administration. Results showed that smaller conventional liposomes, cationic and PEGylated liposomes had longer retention time in digestive tract. Few intact liposomes were taken up by intestinal epithelial cells and none were found in circulation. In vivo pharmacokinetics revealed that the smaller, cationic or PEGylated liposomes had higher relative bioavailability. Similar retention time of various liposomes in blood circulation to control solution indicated that liposomes improved oral drug absorption by either prolonging contact time with gastrointestinal tract or increasing penetration ability through mucus barrier, instead of being absorbed integrally into circulation. This study offered new insight into developing highly effective liposomes for oral delivery.

Mean retention time in digestive tract and digestion of fresh perennial ryegrass by lactating dairy cows: influence of grass maturity and comparison with a maize silage diet

The effects of grass maturity on the mean retention time of liquid and particles, digestibility and duodenal nutrient flows were measured by feeding second cuts of perennial ryegrass to 3 fistulated lactating dairy cows over 2 successive periods (late June and mid-July, 28 and 49 d regrowth, respectively). Fresh forage was given ad libitum with 12% (dry matter (DM) basis) concentrate. The increase in maturity lowered grass nitrogen content, organic matter (OM) digestibility and non-ammoniacal nitrogen flow into the duodenum, but did not affect OM intake (13.2 kg/d). Neither the fractional outflow rate of liquid in the rumen (FOR; 17.3%/h) nor the concentrate total mean retention time (TMRT; 32.9 h) were affected. With maturity forage TMRT tended to increase (43.0-48.8 h), certainly because of the longer comminution time of coarse particles. These results were compared with data obtained 2 months earlier with the same cows fed on a maize silage diet (25% DM basis of the same concentrate) ad libitum (17.4 kg OM/d). Despite a lower total OM intake, FOR was much higher with the grass than with the maize silage diet (12.1%/h) and this may explain the lower retention time in the rumen of the small particles when grass diets were given (19.0 vs 24.3 h for maize silage diet).