Retention Period Research Articles

Effect of Fig on relapse during retention period after orthodontic treatment (Animal study)

Effect of Fig on relapse during retention period after orthodontic treatment (Animal study)

Anaerobic Co-digestion of Palm Oil Sludge, Cassava Peels, Cow Dung and Ground Eggshells: Process Optimization and Biogas Generation

Indiscriminate disposal of crop and animal wastes has grown in acceptance across the globe as an environmentally hazardous practice. This study used a 225L polyethylene digester that was specially made to produce biogas from anaerobic co-digestion of palm oil sludge, cassava peels, and cow dung using ground eggshells for pH stabilization and a greenhouse for temperature control. Cassava peels, palm oil sludge, cow dung, and water were combined in a ratio of 1:1:2:5.3, respectively, and 1.3 kilograms of crushed eggshells were added. The bio-digestion system generated 650.60 L of cumulative biogas throughout the 30-day sludge retention period. The pH averaged 6.0, and the slurry temperature averaged 34.76oC during digestion, which is favorable for the production of biogas since microbial populations thrive under hospitable conditions. The biogas produced after a hydraulic retention time (HRT) of over 20 days had the highest methane concentration of 60%, while days under 10 HRT had the lowest methane content of 45.5%. On the 13th day of anaerobic digestion, biogas output peaked at 34.90L, and pH and temperature were maintained at 6.5 and 35.0°C, respectively, the ideal ranges for a healthy process. An efficient technique for producing energy in the form of biogas was shown by optimized anaerobic co-digestion of animal and crop waste utilizing ground eggshells and a greenhouse for pH and temperature control. Future research should focus on developing more efficient, cheaper microbial agents, such as enzymes for biological pre-treatment of palm oil sludge to reduce lignin, which negatively impacts biogas generation.

Investigation of changes caused by Rapid Maxillary Expansion in mandibular bone and temporomandibular joint trabecular structure using fractal analysis

BackgroundThe aim of this study is to evaluate the changes in the trabecular structure of the mandibular bone and temporomandibular joint following Rapid Maxillary Expansion.MethodsThirty-nine patients who received Rapid Maxillary Expansion treatment in the Department of Orthodontics of the Faculty of Dentistry were selected from the archieve for the study. Trabecular structure changes of the angulus mandible, corpus mandible, and condyle regions were examined using fractal analysis on the dental panoramic radiographs. Radiographs taken before the treatment (T0) and at the end of the 3-month retention period (T1) of the patients.ResultsThere was no statistically significant difference between T0 and T1 in the condyle, ramus mandible, and corpus mandible regions depending on time and sex. (p < 0.05)ConclusionThe results of this study showed that Rapid Maxillary Expansion treatment does not have a significant impact on the trabecular structure of the mandible and temporomandibular joint depending on time and gender.

Biogas Production from a Solar-Heated Temperature-Controlled Biogas Digester

This research paper explores biogas production in an underground temperature-controlled fixed dome digester and compares it with a similar uncontrolled digester. Two underground fixed-dome digesters, one fitted with a solar heating system and a stirrer and the other one with an identical stirrer only, were batch-fed with cow dung slurry collected from the University of Fort Hare farm and mixed with water in a ratio of 1:1. The solar heating system consisted of a solar geyser, pex-al-pex tubing, an electric ball valve, a water circulation pump, an Arduino aided temperature control system, and a heat exchanger located at the centre of the digester. Both the digesters were intermittently stirred for 10 min every 4 h. The digester without a heating system was used as a control. Biogas production in the two digesters was compared to assess the effect of solar heating on biogas production. The total solids, volatile solids, and the chemical oxygen demand of the cow dung used as substrate were determined before and after digestion. These were compared together with the cumulative biogas produced and the methane content for the controlled and uncontrolled digesters. It was observed that the temperature control system kept the slurry temperature in the controlled digester within the required range for 82.76% of the retention period, showing an efficiency of 82.76%. Some maximum temperature gradients of 7.0 °C were observed in both the controlled and uncontrolled digesters, showing that the stirrer speed of 30 rpm was not fast enough to create the needed vortex for a uniform mix in the slurry. It was further observed that the heat from the solar geyser and the ground insulation were sufficient to keep the digester temperature within the required temperature range without any additional heat source even at night. Biogas yield was observed to depend on the pH with a strong coefficient of determination of 0.788 and 0.755 for the controlled and uncontrolled digesters, respectively. The cumulative biogas was 26.77 m3 and 18.05 m3 for controlled and uncontrolled digesters, respectively, which was an increase of 33%. The methane content increased by 14% while carbon dioxide decreased by 10% from the uncontrolled to the controlled scenario. The percentage removal of the TS, VS, and COD was 66.26%, 76.81%, and 74.69%, respectively, compared to 47.01%, 60.37%, and 57.86% for the uncontrolled situation. Thus, the percentage removal of TS, VS, and COD increased by 19.25%, 16.44%, and 16.89%, respectively.

Inhibition of Orthodontic Relapse by Local Application of Simvastatin-Loaded Gelatin Hydrogel in a Rabbit Model.

This study aims to determine the effect of gelatin-simvastatin hydrogel application on bone remodelling during relapse. Twenty-four rabbits (Oryctolagus cuniculus) were divided into a control group (n = 12) and a treatment group (n = 12). The lower incisors were subjected to orthodontic force and moved distally by an open coil spring. The force (50 cN) was extended for 1 week and retained in a new position for 2 weeks (stabilisation period). The treatment group received a local gelatin-simvastatin hydrogel administration during stabilisation, and the control group received only gelatin hydrogel. The springs were debonded after the stabilisation phase from both groups to facilitate a relapse. The percentage of relapse was measured in a model study using digital callipers. Levels of OPG and RANKL were analysed using ELISA. The local application of gelatin-simvastatin hydrogel in the treatment group significantly decreases the relapse percentage and RANKL level. Additionally, the gelatin-simvastatin hydrogel significantly increases the level of OPG and ratio of OPG/RANKL. Applying gelatin-simvastatin hydrogel in the retention period can induce bone formation, inhibit orthodontic relapse and increase tooth stability (in vivo).

The Catalog of Procedures as Part of Recordkeeping

The Regulation of the Government of the Republic of Serbia on recordkeeping of state administration bodies, which applies to other holders of public administration bodies from 2023, adapted recordkeeping to the needs of e-administration. The designed ePisarnica (Serbian erecords centre) as a unique software solution for recordkeeping and managing centralized registry of records, and a unique system for managing documents as a separate software solution, caused a number of changes in the way of managing registries on files in regard to the so far way of recordkeeping. The catalog of procedures is a new element in office business, it is a record of all administrative and non-administrative procedures of administration bodies and entities exercising public powers. The catalog also contains a codebook of records categories with retention periods. The paper will show the development and innovations in recordkeeping and the role of the catalog of proceedings in the central record of cases with special reference to the catalog of proceedings of provincial bodies.

Simultaneous estimation of Flupenthixol and Esitalopram in bulk drug and tablet dosage form by UHPLC method

The objective of this study is to novel ‘high performance liquid chromatography’ method this is sensitivity and accurates, with focus on specificity, for measuring the amount of Flupenthixoland Escitalopramin bulk dosage form and pharmaceutical formulation. Methods: Drug and its impurities were separated chromatographically using C18(AGILENT) coloumn together with a methanol-based mobile phase and 0.1% TFAA (pH4.2 WITH TEA) at a flow rate of one milliliter per min in the ratio 65;35, with UV detection 235nm. Results: With good resolution, Flupenthixol and Esitalopram were successfully eluted during the retention period off 3.044 and 4.118 min, Respectivaly. The described method showed a linear relationship across the flupenthixol concentration range. (200-100ug/ml) and esitalopram (1-5 g/ml). Flupenthixol and Esitalopram were found to have 100%–102% recoveries. When validating the method, the ICH guidelines were adhered to. Excellent precision, accuracy, linearity, specificity, sensitivity, and robustness were demonstrated by the validation results. Conclusion: The proposed method effectively separated and quantified escitalopram and flupentixol. This developed technique is totally analysissure suitable for routine analysis ofEscitalopram and Flupenthixol in dosage of pharmaceuticals formulation.

The perceptual and mnemonic effects of ensemble representation on individual size representation.

Our visual world consists of multiple objects, necessitating the identification of individual objects. Nevertheless, the representation of visual objects often exerts influence on each other. Even when we selectively attend to a subset of visual objects, the representations of surrounding items are encoded and influence the processing of the attended item(s). However, it remains unclear whether the effect of group ensemble representation on individual item representation occurs at the perceptual encoding phase, during the memory maintenance period, or both. Therefore, the current study conducted visual psychophysics experiments to investigate the contributions of perceptual and mnemonic bias on the observed effect of ensemble representation on individual size representation. Across five experiments, we found a consistent pattern of repulsive ensemble bias, such that the size of an individual target circle was consistently reported to be smaller than it actually was when presented alongside other circles with larger mean size, and vice versa. There was a perceptual component to the bias, but mnemonic factors also influenced its magnitude. Specifically, the repulsion bias was strongest with a short retention period (0-50 ms), then reduced within a second to a weaker magnitude that remained stable for a longer retention period (5,000 ms). Such patterns of results persisted when we facilitated the processing of ensemble representation by increasing the set size (Experiment 1B) or post-cueing the target circle so that attention was distributed across all items (Experiment 2B).

Survey - Based Study For Common Retention Practices Among Orthodontists In North Macedonia

Background: Orthodontic therapy always ends with retention to prevent the return of the final occlusal outcome and to maintain the teeth in the correct position after the end of orthodontic therapy. This research aimed to survey retention practices, factors affecting retention protocol and differences regarding retention among orthodontists in North Macedonia. Material and methods: 150 questionnaire copies were distributed by mail or personally to orthodontics in North Macedonia. The survey included 24 single or multiple-answer questions, about retention system, frequently used fixed and removable retainers in certain malocclusions, the duration of the retention period, retainer supervision, instructions for patients, and the need for retention guidelines in order to determine habits and knowledge, and reasons for choosing a particular retention regime. Results : Out of 150 questionnaires, 93 were appropriately filled out and returned, so response rate was 62%. 90% give oral information about retention, only 25% informed their patients both oral and written. The most preferred retainer in maxilla (54%) was vacuum-formed appliance, than removable acrylic plate appliance (41%) and only 9% bonded retainer. In mandible, mostly used are VFR (45%), then acrylic plate appliance (29%), bonded retainers (20%). Dual retention was used more in upper jaw. The retention period was 4 to 5 years or lifelong; the choice of retention device in 77% depended on malocclusion. The reason for the choice of VFR are availability (50,5%), quality (36,56%), low-cost (33,3%). Protocol was influenced by clinical experience (75%), knowledge from residency (58%), courses (56%) and from colleagues (40%). 83% made changes in retention protocol in past years, in appliance time (20%) or in retention period (19%). 95,7% of the surveyed orthodontists believe that general guidelines for retention are needed. Conclusions: The most preferred retainers in North Macedonia were vacuum-formed appliance, than removable acrylic plate appliance and at least bonded retainer. Combination of fixed and removable appliance in mandible were used less than in upper jaw. Reason for choosing was malocclusion. Retention protocols are influenced mostly by the clinical experience. Common retention guidelines are required for most orthodontists.

Serial engagement of distinct motor learning mechanisms to alter walking after stroke

This study asked if combining different motor learning mechanisms—adaptation and reinforcement—could produce immediate improvements in over ground walking after stroke. Fifteen adults with stroke engaged in three conditions: (1) reinforcement following adaptation, (2) reinforcement alone, and (3) adaptation alone. Adaptation involved split-belt treadmill walking to produce after-effects that reduce step asymmetry. Reinforcement involved the use of real-time auditory feedback about step length asymmetry. Auditory feedback was binary, signaling whether steps were asymmetric or equal, but not whether to shorten or lengthen either step. Change in step length asymmetry was the outcome assessed during over ground walking. Reinforcement following adaptation led to reductions in step length asymmetry that persisted into an immediate retention period. Importantly, it led to the desired pattern of lengthening the shorter step in a majority of participants. Reinforcement alone led to no significant change in step length asymmetry, and sometimes produced a non-optimal pattern of shortening the longer step. Our control condition of adaptation alone led to more transient reductions in step length asymmetry. These findings reveal the potential for utilizing serial delivery of adaptation and reinforcement to influence a complex movement in the real-world context of over ground walking, in people with stroke.

Poly-Ether-Ether-Ketone versus dead-soft coaxial bonded retainers: a randomized clinical trial. Part 2: periodontal health and microbial biofilm assessment.

Retainers have the potential to detrimentally impact periodontal health and contribute to tooth decay. To investigate periodontal health and bacterial biofilm related to Poly-Ether-Ether-Ketone (PEEK) fixed retainers as compared to Dead-soft coaxial fixed retainer (DSC). A two-arm parallel groups single-centre randomized clinical trial. The trial included patients whose orthodontic treatment was completed and required retainers. Participants were randomly assigned into two retainer groups: PEEK retainers, prepared by computer-aided design and manufacturing into 0.8mm wire form, and DSC retainers. The objectives included assessing periodontal health through plaque accumulation index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD), gingival index (GI), calculus index (CI), and alveolar bone height (ABH) assessment. Biofilm assessment involved bacteriological screening of aerobic, facultative anaerobic, mutans streptococci, and lactobacilli. The periodontal indices and microbiological screening as well as were assessed at the debonding stage (T0), 1-month (T1), 3-month (T3), and 6-month (T6) after the commencement of the trial, except for the ABH, which was recorded using periapical radiograph at T0 and T6. Single blinding of participants in addition to the bacteriological specialist. Initially, the trial enrolled 46 participants, aged between 12 and 28 years, and were randomly assigned to two groups, with 23 participants in each group. Subsequently, one participant withdrew from the trial, resulting in a total of 45 participants whose data were analysed. Assessment of the periodontal indices, excluding the CI (P = .480), revealed statistically but not clinically significant differences between groups after 6-month of retention (P = .016 of PI, P = .020 of BOP, P = .05 of PPD, and P = .01 of GI). There was slight plaque accumulation, normal PPD (approximately 1mm), healthy to mild gingivitis with a GI of less than 1 and BOP was around 10%. Concerning the ABH, there was a noticeable reduction in its score after 6 months, particularly in the PEEK group, although the difference was not statistically significant (P = .102). Furthermore, the bacteriological viable count did not show any significant difference between the groups during the recall visits. There have been no reported negative consequences. Blinding the assessor of periodontal indices was not feasible due to the nature of the intervention. The trial follow-up duration was limited. Both the PEEK and DSC retainers have comparable impacts on periodontal health and bacterial accumulation and composition during the retention period. NCT05557136.

PEEK Retainers without CAD-CAM: Simple Solutions for Everyday Challenges

Background: The need to perform occasional or continuous MRI exams and the interference with metal orthodontic appliances might be important and take a primary role during retention since the retention period is significantly longer than orthodontic treatment. Several non-metallic materials were proposed as potential alternatives to perform fixed retainers in orthodontics, but they showed internal limits. Methods: Polyetheretherketone (PEEK) was used in the present clinical report as a fixed orthodontic retainer in the lower arch in order to perform an appliance with mechanical properties comparable to metallic ones but with a higher biocompatibility material and without the need for removal in case of an MRI exam. The retainer wire was handmade in the studio and then shaped to fit the arch. Results: PEEK showed a good capability for constructing a lingual fixed retainer compared to other aesthetic non-metallic and metallic materials. Conclusions: To the best of our knowledge, this study proposes how to easily build a retainer in PEEK and provides a clinical example of how this material can be beneficial.

A novel approach for posterior bite collapse in an adult with crossbite anterior using a 3D-printed bite riser

Background: Managing an adult patient presenting with an anterior crossbite in conjunction with posterior bite collapse (PBC) is a difficult challenge. Purpose: The purpose of this case study is to highlight the accuracy that can be attained with the utilization of a three-dimensional (3D) printed bite riser in the correction of PBC accompanied by an anterior crossbite in an adult patient with Class III malocclusion. Case: A 64-year-old male complained of being unable to chew properly. Extraoral examination revealed a concave profile with a protrusive mandible. The patient exhibits a 5 mm anterior crossbite and has missing lower posterior molars, resulting in bilateral PBC. The cephalometric analysis revealed skeletal Class III patterns (SNA: 82.19o; SNB: 86.34o; ANB: -4.15°), with protrusion and counterclockwise rotation of the mandible (SN-OP: 3.84o). Case Management: By utilizing a 3D-printed bite riser in conjunction with temporary anchoring devices (TADs), it is possible to resolve the issue of an anterior crossbite accompanied by PBC. This is achieved by repositioning the elongated posterior segments on both sides of the maxilla to generate vertical space for mandibular tooth rehabilitation, retracting the anterior segment, and narrowing the lower arch. Simultaneously, the crossbite on both sides was corrected, a Class I and ideal overbite and overjet were achieved, the occlusal vertical dimension increased, the occlusal plane angle was corrected (7.51o), and an acceptable ANB angle (-1.36°) was accomplished. The treatment lasted 18 months and yielded sustained results after a one-year retention period. Conclusion: Integrating 3D printing technology in orthodontic treatment offers numerous options for managing challenging cases such as PBC while also reducing the treatment length. The patient was satisfied with the results achieved.

Rhythm Facilitates Auditory Working Memory via Beta-Band Encoding and Theta-Band Maintenance.

Rhythm, as a prominent characteristic of auditory experiences such as speech and music, is known to facilitate attention, yet its contribution to working memory (WM) remains unclear. Here, human participants temporarily retained a 12-tone sequence presented rhythmically or arrhythmically in WM and performed a pitch change-detection task. Behaviorally, while having comparable accuracy, rhythmic tone sequences showed a faster response time and lower response boundaries in decision-making. Electroencephalographic recordings revealed that rhythmic sequences elicited enhanced non-phase-locked beta-band (16 Hz-33 Hz) and theta-band (3 Hz-5 Hz) neural oscillations during sensory encoding and WM retention periods, respectively. Importantly, the two-stage neural signatures were correlated with each other and contributed to behavior. As beta-band and theta-band oscillations denote the engagement of motor systems and WM maintenance, respectively, our findings imply that rhythm facilitates auditory WM through intricate oscillation-based interactions between the motor and auditory systems that facilitate predictive attention to auditory sequences.

Improving dairy manure hydrolysis and acidification through microbial community restructuring by adaptation to hyperthermophilic conditions

Dairy manure (DM) contributes significantly to greenhouse gas emissions and ecosystem degradation, yet its resistance to biodegradation hinders widespread bioprocessing applications. Lignocellulosic materials in DM pose a particular challenge because of their recalcitrance. Bioprocessing under hyperthermophilic (≥70 °C) conditions potentially offers an advantage over traditional fermentation temperatures due to enhanced activity of enzymes and the kinetics of enzymatic reactions. This can lead to a higher conversion rate and a greater extent of biomass hydrolysis and acidification. To test the validity of this hypothesis, the current study evaluated the efficacy of anaerobic hydrolysis and acidogenic fermentation of DM under mesophilic, thermophilic, and hyperthermophilic conditions. All inocula were adapted to corresponding temperatures but were derived from the same mesophilic source. Hyperthermophilic conditions resulted in superior DM hydrolysis efficiency (53%) compared to mesophilic (34%) and thermophilic (42%) conditions. The hyperthermophilic environment was particularly favorable to the decomposition of crude proteins and hemicellulose, which were reduced by 64% and 54%, respectively. Furthermore, hyperthermophilic fermentation also yielded the highest volatile fatty acid (VFA) production rate of 460 mg/L/day during the first four days, representing improvements of 50% and 90% over mesophilic and thermophilic conditions. In part, this was attributed to the enhanced production of branched-chain VFAs, including an increase of 6–10% in isobutyric acid and 12–13% in isovaleric acid. At hyperthermophilic conditions, however, there was no accumulation of VFAs during the days 5–8 of fermentation, which could be due to acetate conversion by the syntrophic acetate-oxidizing bacteria. A considerable gain in hydrolysis efficiency and VFA production rate were accompanied by a reduction in microbial diversity, which suggests that hyperthermophilic temperature is a favorable environment for the selection of organisms with enhanced DM hydrolysis and fermentation capabilities. A significantly increased relative abundance of xylanolytic Caldicoprobacter (23% of population) and proteolytic Thermovirga (9% of population) could be the major contributors to improved decomposition of hemicellulose and protein. As revealed by the techno-economic analysis, acidogenic fermentation of DM at 70 °C and a retention period of 4 days provides the greatest positive net present value, highest internal rate of return of 9.2%, and shortest investment payback period of 9 years. This study demonstrates that hyperthermophilic conditions enable superior deconstruction and bioconversion of lignocellulose-containing biomass into VFAs under reduced retention times, offering a promising approach for improving DM management and generating bioproducts.

Class III correction and enhanced periodontal health with aligner treatment in a 53-year-old patient.

In this case report, we describe the successful camouflage treatment of a 53-year-old female with dental and skeletal Class III malocclusion combined with anterior crossbite, gingival recession, and mobility of the lower incisors, using clear aligners. The treatment involved periodontal debridement followed by orthodontic treatment. The mandibular posterior teeth were distalized to correct the anterior crossbite and to establish Class I molar relationships. During treatment, the mandibular incisors were intruded, and the mandibular occlusal plane underwent a clockwise rotation due to slight extrusion of the maxillary buccal segments and distalization of the mandibular posterior teeth. Following treatment, all objectives were achieved, including resolution of the anterior crossbite, significant reduction of gingival recession and tooth mobility, and improved functional occlusion. The dental and skeletal Class III malocclusion was corrected, and the marginal alveolar bone dehiscence was significantly reduced. The results remained stable over a 3-year retention period, with enhanced molar intercuspation and gingival growth progression. This case adds to the evidence supporting the adaptability and effectiveness of clear aligners in treating orthodontic patients with compromised periodontium. The treatment outcomes support that orthodontic treatment using clear aligners, combined with periodontal monitoring, can assist in managing alveolar bone defects, gingival recession, and tooth mobility.

Investigation of bone remodeling in gingival crevicular fluid in patients treated with rapid maxillary expansion during pubertal period

Purpose: We aimed to evaluate bone remodeling following 3-month and 6-month retention periods of rapid maxillary expansion (RME) in gingival crevicular fluid (GCF). Materials and Methods: 23 pubertal participants (15 girls- 8 boys, 12-15 years) with maxillary transversal deficiency were enrolled in the study. Following banded-type RME appliance was introduced into the mouth, RME protocol was initiated by turning the Hyrax screw twice daily (morning/evening) with ¼ activation. GCF samples were taken from right maxillary first molars at four different time points (T0: before the appliance was deployed, T1: following active phase, T2: at the end of the 3-month retention period, T3: at the end of the 6-month retention period) using paper strips. Probing depth, gingival index, plaque index, and bleeding percentage at probing were recorded. BALP, OPG, RANKL, and TNF-α levels were measured in GCF samples using ELISA kits. Results: Our study revealed that GCF's BALP and OPG levels remained unchanged over time in tension and pressure regions, and there were no statistically significant differences among regions at each time point (p&gt;0.05). RANKL level was statistically significantly increased in the buccal region (the pressure region) at time T1 (p=0.042). The palatinal region's TNF-α level was statistically significantly higher than the buccal region at T0, T2, and T3 time points (p=0.005, p=0.042, and p=0.006, respectively). Our study showed no correlation between 3-month and 6-month retention periods and biomarkers indicating RME's bone metabolic activities. Conclusions: Further studies with different retention periods and larger sample sizes are suggested.

Research on Key Parameters for Relieving Pressure on Roofs of Deep Mine Cutting and Retaining Roadways

Scientific and reasonable roof-cutting parameters are key to ensuring pressure relief of the retained roadway roof. This manuscript takes the 7135 working face of Qidong Coal Mine as the engineering background and uses theoretical analysis, numerical and on-site measurement methods to study the quantitative relationship and pressure relief effect between different roof-cutting parameters of GERRC. We established a fracture criterion based on the tensile strength of the main roof of the uncut joint along the cutting line. We analyzed the quantitative relationship between different main roof thickness, cutting height, cutting angle, and the main roof tensile stress of the uncut joint. We found that within a small range of cutting angles, as the cutting angle decreases, the tensile stress on the main roof of the uncut joint increases. When the cutting angle is 0, the main roof tensile stress of the uncut joint reaches its maximum. As the cutting height increases, the limitation of the cutting angle on the cutting height becomes smaller. Numerical simulation was conducted to study the distribution patterns of maximum and minimum principal stresses along the direction of the roadway roof during the retention period under different roof-cutting heights and angles. Based on this, the optimal unloading effect of the roadway roof and the minimum concentration of mining stress were obtained at a roof-cutting height of 9 m and a roof-cutting angle of 80°. Through on-site measurement of the stress on the reinforcement anchor cable during the retention period, the deformation of the sinking roadway roof, and the pressure relief control effect of the retained roadway roof, the pressure relief effect and scientific rationality of the design of the cutting height and cutting angle were verified.

Mark-recapture validates the use of photo-identification for the widely distributed blue-spotted ribbontail ray, Taeniura lymma

The ability to identify individual animals can provide valuable insights into the behaviour, life history, survivorship, and demographics of wild populations. Photo-identification (photo-ID) uses unique natural markings to identify individuals and can be effective for scalable and non-invasive research on marine fauna. The successful application of photo-ID requires that chosen distinguishing markings are unique to individuals and persist over time. In this study, we validate the use of dorsal spot patterns for identifying individual blue-spotted ribbontail rays (Taeniura lymma) in conjunction with traditional tagging methods. Spot patterns were unique among T. lymma with 90.3% of individuals correctly identified using I3S photo-matching software from images taken up to 496 days apart. In comparison, traditional physical tagging methods showed a tag loss rate of 27% and a maximum tag retention period of only 356 days. Our findings demonstrate the effectiveness of photo-ID as a tool to monitor populations and better understand the ecology of the blue-spotted ribbontail ray without the need for physical tagging. The validation of photo-ID for this widespread species is important as it enables behavioural and demographic changes to be easily tracked in relation to coastal threats such as human development and habitat degradation.

Does krill oil enhancing the new bone formation in orthopedically expanded median palatal suture in rat model? A micro-CT and immunohistochemical analysis

BackgroundThe purpose of this study was to assess the effects of systemically given krill oil (KO) on the development of new bone formation in the sutura palatina media following rapid maxillary expansion (RME).Methods28 4–5 week-old male Wistar albino rats were randomly divided into 4 groups: Control (C), Only Expansion (OE) (no supplement but undergoing expansion and retention), KE (supplemented during both the expansion and retention phases), Krill Oil Nursery Group (KN) (supplemented during the 40-day nursery phase as well as during the expansion and retention phases). A 5-day RME was followed by a 12-day retention period. All rats were euthanized simultaneously. Micro-computerized tomography (Micro-CT), hemotoxylen-eosin (H&E) staining, and immunohistochemical analysis were conducted. Kruskal-Wallis and Dunn tests with Bonferonni corrrection were applied (p < 0.05).ResultsExpansion and KO supplementation did not cause a statistically significant change in bone mineral density (BMD), bone volume fraction (BV/TV), spesific bone surface (BS/BV) and trabecular thickness (Tb.Th). While the expansion prosedure increased the trabecular seperation (Tb.Sp), KO supplemantation mitigated this effect. The KE group exhibited a statistically significantly increase in trabecular number (Tb.N) compared to the OE group. Although receptor activator of nuclear factor-kappa-Β ligand (RANKL)/osteoprotegerin (OPG) ratios did not show significant differences between groups, the KE and OE groups demonstrated the lowest and highest value, respectively. KE showed a reduced amount of tartrate-resistant acid phosphatase (TRAP) compared to the OE.ConclusionKO positively affected the architecture of the new bone formed in the mid-palatal suture. In this rat model of RME, results support the idea that administering of KO during the expansion period or beginning before the RME procedure may reduce relapse and enhance bone formation within the mid-palatal suture.