This study presents a new knock control criterion based on different loads and engine power output loss. By utilizing a modified port fuel injection (PFI) spark-ignition (SI) methanol marine engine, the suppressive effects of retarding ignition timing, reducing air-fuel ratio and delaying injection timing on knock intensity at different loads were investigated. Subsequently, a comparative analysis of three knock control strategies was conducted to determine their impacts on power output and thermal efficiency, thus proposing the optimal knock control strategy at different loads. The results show that at both low load (MAP = 73 kPa) and high load (MAP = 139 kPa), the high knock intensity in KLSA (knock-limited spark advance) +5 case is reduced to the safety limitation by retarding ignition timing and reducing air-fuel ratio, while retarding injection timing can effectively suppress knock only at low load. Furthermore, under KLSA+5 condition, there is a significant increase in the probability of severe knock for CA05 earlier than the knock boundary. As the knock tendency is mitigated, IMEP reduces monotonically. There is lower power output loss by optimizing injection timing and air-fuel ratio at low load, and optimizing ignition timing at high load, while the air dilution strategy consistently results in minimal thermal efficiency loss.