Resveratrol Consumption Research Articles

Synergistic Role of Aerobic Training and Resveratrol on AMPK/PGC1-α/SIRT1 Pathway in the Hippocampus of Rats with Alzheimer's Disease

Background: Alzheimer's disease symptoms indicate that increased Aβ leads to central nervous system dysfunction. Exercise reduces Aβ deposition through the AMPK signaling pathway. Additionally, resveratrol (RSV) has neuroprotective effects related to cognitive decline. Objectives: This study investigated the effects of aerobic training and RSV on the AMPK/PGC-1α/SIRT1 pathway in the hippocampus of Alzheimer's rats. Methods: Thirty-five male Wistar rats were divided into five groups: Normal (NO), Alzheimer's (AD), Alzheimer's-training (ADT), Alzheimer's-resveratrol (ADRSV), and Alzheimer's-training-resveratrol (ADTRSV). The supplement groups received 20 mg of RSV (per kg of body weight) orally during the intervention period. The aerobic exercise program, which included running on a treadmill at speeds of 6 - 18 meters per minute, was performed 5 days a week for eight weeks. Results: Alzheimer's induction caused a significant decrease in the expression of AMPK/PGC-1α/SIRT1 (P = 0.0001). Exercise and RSV significantly increased the expression of AMPK/PGC-1α/SIRT1 in AD rats (P < 0.05). Additionally, significant increases in the expression of AMPK, PGC-1α, and SIRT1 were observed in the ADTRSV group compared to the ADT group (P = 0.034, P = 0.020, and P = 0.038, respectively) and ADRSV (P = 0.026, P = 0.021, and P = 0.021). Conclusions: AD induction was associated with decreased AMPK/PGC-1α/SIRT1 expression. Aerobic exercise and RSV consumption can reverse this decrease. Given the crucial role of this signaling pathway in hippocampal function, alterations in these indicators following physical activity and RSV use may partially mitigate Alzheimer's disease complications.

Resveratrol delays the progression of diabetic nephropathy through multiple pathways: A dose-response meta-analysis based on animal models.

Accumulating experimental evidence has shown that resveratrol supplementation is effective for treating diabetic nephropathy (DN) in animal models. In this systematic review and meta-analysis, we assessed the effects and multiple mechanisms of resveratrol in animal models of DN. Before September 2023, preclinical literature was systematically searched and screened across PubMed, Web of Science, EMBASE, and the Cochrane Library. Forty-two studies were included, and the risk of bias tool from SYRCLE was used to assess the methodological quality. Pooled overall effect sizes of the results were generated by STATA 16.0. The overall results provide preliminary evidence that the consumption of resveratrol can significantly reduce the mesangial index, glomerular basement membrane thickness, glomerular hypertrophy, serum creatinine, blood urea nitrogen, 24-h urinary protein, blood glucose, kidney index, total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels. In contrast, the levels of albumin and high-density lipoprotein cholesterol are significantly increased. However, resveratrol did not significantly reduce creatinine clearance or glycated hemoglobin levels. Dose-response analysis revealed that resveratrol was most effective at improving kidney function and reducing DN when administered at lower doses of ≤15 mg/kg/day or higher doses of 100-200 mg/kg/day, with significant improvements in biochemical kidney injury markers and a better effect on dysglycemia. The benefits of resveratrol in DN are likely due to its anti-inflammatory, antioxidant, metabolic regulatory, and autophagy-promoting effects. To confirm these findings for clinical use, further large-scale, long-term, high-quality preclinical trials are warranted to accurately assess the anti-DN effects and safety of resveratrol.

Resveratrol ameliorates mitochondrial biogenesis and reproductive outcomes in women with polycystic ovary syndrome undergoing assisted reproduction: a randomized, triple-blind, placebo-controlled clinical trial

BackgroundThis study was designed to examine the effect of resveratrol on mitochondrial biogenesis, oxidative stress (OS), and assisted reproductive technology (ART) outcomes in individuals with polycystic ovary syndrome (PCOS).MethodsFifty-six patients with PCOS were randomly assigned to receive 800 mg/day of resveratrol or placebo for 60 days. The primary outcome was OS in follicular fluid (FF). The secondary outcome involved assessing gene and protein expression related to mitochondrial biogenesis, mitochondrial DNA (mtDNA) copy number, and adenosine triphosphate (ATP) content in granulosa cells (GCs). ART outcomes were evaluated at the end of the trial.ResultsResveratrol significantly reduced the total oxidant status (TOS) and oxidative stress index (OSI) in FF (P = 0.0142 and P = 0.0039, respectively) while increasing the total antioxidant capacity (TAC) (P < 0.0009). Resveratrol consumption also led to significant increases in the expression of critical genes involved in mitochondrial biogenesis, including peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) and mitochondrial transcription factor A (TFAM) (P = 0.0032 and P = 0.0003, respectively). However, the effect on nuclear respiratory factor 1 (Nrf-1) expression was not statistically significant (P = 0.0611). Resveratrol significantly affected sirtuin1 (SIRT1) and PGC-1α protein levels (P < 0.0001 and P = 0.0036, respectively). Resveratrol treatment improved the mtDNA copy number (P < 0.0001) and ATP content in GCs (P = 0.0014). Clinically, the resveratrol group exhibited higher rates of oocyte maturity (P = 0.0012) and high-quality embryos (P = 0.0013) than did the placebo group. There were no significant differences between the groups in terms of chemical or clinical pregnancy rates (P > 0.05).ConclusionsThese findings indicate that resveratrol may be a promising therapeutic agent for patients with PCOS undergoing assisted reproduction.Trial registration numberhttp://www.irct.ir; IRCT20221106056417N1; 2023 February 09.

Single or combined consumption of resveratrol and the probiotic, Lactobacillus acidophilus attenuate the effects of crowding stress on growth, immune characteristics, and antioxidant defense in the common carp, (Cyprinus carpio)

In the present study, dietary resveratrol (RE) and Lactobacillus acidophilus (LAB) were individually or combined added to the diet of common carp (Cyprinus carpio) to protect against crowding stress. Fish (30.16 ± 0.7; Mean ± SE) were randomly allocated to seven groups in three replicates, as follows: T1: basic food as control, T2: LAB with a concentration of 1.5 × 107 CFU/g, T3: LAB with a concentration of 3 × 107 CFU/g, T4: 300 mg resveratrol/kg, T5: 600 mg resveratrol/kg, and T6: 1.5 × 107 CFU/g + 300 mg resveratrol/kg and T7: 3 × 107 CFU/g + 600 mg resveratrol/kg. After 60 days feeding, the supplemented fish had the highest final body weight (FBW), weight gain (WG), and specific growth rate (SGR), and the lowest feed conversion ratio (FCR) as compared with the control group (P < 0.05). The activities of amylase, protease and lipase were noticed markedly higher in fish supplemented with 1.5 × 107 CFU/g + 300 mg resveratrol/kg and 1.5 × 107 CFU/g diets compared to the control (P < 0.05). Generally, fish in supplemented diets, particularly T2 and T6 groups, had the highest lysozyme, alternative complement activity (ACH50), total immunoglobulin (Ig), nitroblue tetrazolium test (NBT), myeloperoxidase (MPO), complement component 3 (C3), complement component 4 (C4), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lower levels of malondialdehyde (MDA), glucose, cortisol, alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) when were compared with the control before crowding stress (P < 0.05). After crowding stress challenge, fish in the supplemented groups, particularly T2 and T6, generally showed significantly higher values of lysozyme, ACH50, total Ig, NBT, MPO, C3, C4, SOD, CAT, GPx and lower levels of MDA, glucose, cortisol, ALT, ALP, LDH when compared with the control (P < 0.05). Also, recovered fish in the control group demonstrated significantly declined levels of lysozyme, ACH50, total Ig, NBT, MPO, C3, C4, SOD, CAT, GPx and higher levels of MDA, glucose, cortisol, ALT, ALP, LDH as compared to other group (P < 0.05). In conclusion, a combined administration of RE and LAB effectively improved growth performance and health status as well as protected common carp against crowding stress.

LC-IMS-HRMS for identification of biomarkers in untargeted metabolomics: The effects of pterostilbene and resveratrol consumption in liver steatosis, animal model

Untargeted metabolomics with the combination of ion mobility separation coupled to high resolution mass spectrometry (IMS-HRMS) was applied to investigate the impact of resveratrol and pterostilbene supplementation on the metabolic fingerprint of the Wistar rats liver with induced liver steatosis. RP-LC and HILIC in both ionisation modes were employed to analyse the liver samples (n = 40) from Wistar rats fed with a high-fat and high-fructose diet, supplemented or not with resveratrol and pterostilbene. After univariate and multivariate statistical analysis, 34 metabolites were highlighted in the different diets and elucidated. Despite the structural similarity, different alterations in liver metabolism were observed by the supplementations. Resveratrol treatment was characterised by the alteration in metabolism of 17 lysophospholipids, while pterostilbene affected some vitamins and derivatives, among others. IMS has demonstrated great potential in the elucidation process thanks to the additional structural descriptor the CCS (Å2), providing more confidence in the identification.

The Effect of Swimming and Resveratrol on Antioxidant Markers and Lipid Peroxidation Index in Liver Tissue

Background Aging is associated with metabolic disorders. Although the beneficial effects of exercise and resveratrol consumption in the health of the elderly has been reported, the combined effect of intermittent swimming and resveratrol consumption on the antioxidant system in liver tissue is not clear yet. Objective This study aims to investigate the combined effect of intermittent swimming and resveratrol consumption on the expression of superoxide dismutases (SOD), glutathione peroxidase (GPx), and Malondialdehyde (MDA) in the liver tissue of older rats. Methods In this experimental study, 32 elder rats were divided into four groups: control, swimming, resveratrol and swimming+resveratrol. The resveratrol (100 mg) dissolved in 1% methylcellulose was administrated daily by gavage. The swimming was performed at three sessions per week. The GPx and SOD expression levels were measured by real-time polymerase chain reaction method, and the MDA level was measured by ELISA method. Results The GPx level in the swimming+resveratrol group were significantly higher than in the control (P=0.001), resveratrol (P=0.04) and swimming (P=0.002) groups. MDA levels in the resveratrol (P=0.001), swimming (P=0.001) and swimming+resveratrol (P=0.001) groups were significantly lower than in the control group; in the swimming+resveratrol group, it was significantly lower than the resveratrol (P=0.001) and swimming (P=0.009) groups. Conclusion It seems that intermittent swimming and resveratrol consumption have favorable effects on improving the antioxidant defense system and lipid peroxidation in liver tissue. Therefore, it is recommended to use intermittent swimming and resveratrol to prevent liver damage in the elderly.

Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream: A Human Pharmacokinetic Study.

Exosomes are extracellular vesicles (EVs) that regulate intercellular signaling by transferring small RNAs, proteins, nucleic acids, lipids, and other metabolites to local or distant organs, including the brain, by crossing the blood–brain barrier. However, the transport of (poly)phenols in human EVs has not yet been described. Therefore, we aimed here to explore (i) whether resveratrol and (or) its derived metabolites are found in the cargo of human plasma exosome-containing EVs (E-EVs), (ii) when this incorporation occurs, and (iii) whether resveratrol intake stimulates the release of E-EVs. Thus, in a pharmacokinetic study, healthy volunteers (n = 16) consumed 1 capsule (420 mg resveratrol) in the evening before attending the clinic and one more capsule on the day of the pharmacokinetics. The plasma and the isolated E-EVs were analyzed using UPLC-ESI-QTOF-MS. Of 17 metabolites in the plasma, 9 were identified in the E-EVs, but not free resveratrol. The kinetic profiles of resveratrol metabolites were similar in the plasma and the E-EVs, a higher metabolite concentration being detected in the plasma than in the E-EVs. However, the plasma/E-EVs ratio decreased in the gut microbial metabolites, suggesting their better encapsulation efficiency in E-EVs. In addition, glucuronide conjugates of resveratrol, dihydroresveratrol, and lunularin were incorporated into the E-EVs more efficiently than their corresponding sulfates despite glucuronides reaching lower plasma concentrations. Notably, more E-EVs were detected 10 h after resveratrol consumption. This exploratory study provides the first evidence that (i) resveratrol metabolites are transported by E-EVs, with a preference for glucuronide vs. sulfates, (ii) the gut microbial metabolites concentration and kinetic profiles are closely similar in E-EVs and plasma, and (iii) resveratrol intake elicits E-EVs secretion. Overall, these results open new research avenues on the possible role of E-EVs in (poly)phenol health effects.

Pilot project. Resveratrol intake by physical active and sedentary older adult women and blood pressure.

Although the use of resveratrol is widespread, there is a lack of studies concerning its use in older adults who practice regular physical activity. The present study aimed to evaluate how resveratrol influenced anthropometric parameters, heart measures, blood analytes in women participants, aged 60 to 80years old, distributed in four groups consisting of those who regularly exercise or not, and those who were under resveratrol treatment or not. The older adult women exercised in a Community Center. A prospective, comparative clinical pilot study was carried out based on convenience non-probability sampling. Resveratrol 300mg/daily/60days was orally-administered to 43 participants that were regularly accompanied and checked for anthropometrical parameters, heart measures, blood analytes, leukogram, and plaquetogram indices. The effect of resveratrol and exercise was not significant in most of the parameters evaluated in the study. Nonetheless, both systolic (p=0.0120) and diastolic (p=0.0241) blood pressures (BP) were elevated in sedentary older adult women in comparison with older women that regularly exercised. However, they remained within the normal BP range considered for the age. Mean corpuscular volume (MCH; p=0.0198) and red blood cell distribution width (RDW; p<0.0001) were improved in the groups receiving resveratrol and exercises compared to the sedentary group receiving resveratrol. The sedentary group receiving resveratrol showed diminished segmented cells (p=0.0459), basophils (p<0.0001), and lymphocytes (p<0.0001). Present findings showed that resveratrol consumption for sedentary older women could lead to dysregulated blood pressure. Although resveratrol consumption is indicated to treat inflammation, its use must be discussed with a health professional and not be inadvertently self-administered due to significant alterations in blood pressure in older adult women, primarily if not related to exercise practice.

Resveratrol in Treating Diabetes and Its Cardiovascular Complications: A Review of Its Mechanisms of Action.

Diabetes mellitus (DM) is one of the most prevalent chronic diseases worldwide. High morbidity and mortality caused by DM are closely linked to its complications in multiple organs/tissues, including cardiovascular complications, diabetic nephropathy, and diabetic neuropathy. Resveratrol is a plant-derived polyphenolic compound with pleiotropic protective effects, ranging from antioxidant and anti-inflammatory to hypoglycemic effects. Recent studies strongly suggest that the consumption of resveratrol offers protection against diabetes and its cardiovascular complications. The protective effects of resveratrol involve the regulation of multiple signaling pathways, including inhibition of oxidative stress and inflammation, enhancement of insulin sensitivity, induction of autophagy, regulation of lipid metabolism, promotion of GLUT4 expression, and translocation, and activation of SIRT1/AMPK signaling axis. The cardiovascular protective effects of resveratrol have been recently reviewed in the literature, but the role of resveratrol in preventing diabetes mellitus and its cardiovascular complications has not been systematically reviewed. Therefore, in this review, we summarize the pharmacological effects and mechanisms of action of resveratrol based on in vitro and in vivo studies, highlighting the therapeutic potential of resveratrol in the prevention and treatment of diabetes and its cardiovascular complications.

Nanomolar resveratrol reduces early alterations of pancreatitis and pancreatic cancer in pancreatic acinar cells

Resveratrol is a natural phytoalexin present in human diet with a potential beneficial effect in many diseases including chronic and acute pancreatitis, a potentially fatal disease with significant morbimortality accompanied with obscure pathogenesis and without effective treatments. Resveratrol has also been shown to be a promising anti-tumor molecule on the millimolar scale. In this study, the protective effects of nanomolar concentrations of resveratrol against early events common to pancreatic cancer and pancreatitis induced by supra-physiological concentrations of cholecystokinin (CCK, 100 nM) were evaluated. Resveratrol (200, 500 nM) reduced CCK-induced intracellular trypsin activation and cell injury in isolated rat pancreatic acinar cells (PAC). The damage induced by high CCK and the protective effect of resveratrol was also measured in pancreatic tissue explants by analyzing histological changes. Finally, the effect of resveratrol on the CCK-enhanced nuclear factor κB (NF-κB) expression was studied by Western blot experiments in the AR42J pancreatic acinar cell line. Our results show, for the first time, the ability of resveratrol to reduce premature intracellular activation of trypsin and necrosis in PAC. It is suggested that resveratrol counteracts CCK-induced pancreatic tissue breakdown by downregulation of NF-κB in the nuclear fraction. Since these effects occur at nanomolar concentrations that can be achieved by oral intake, our results suggest that dietary consumption of resveratrol could have a preventive effect and delay the evolution of pancreatitis and pancreatic cancer.

Sub-chronic cadmium and lead compound exposure induces reproductive toxicity and development of testicular germ cell neoplasia in situin murine model: Attenuative effects of resveratrol.

Cadmium and lead are widespread, nonbiodegradable heavy metals of perpetual environmental concerns. The present study aimed to evaluate whether sub-chronic exposure to cadmium chloride (CdCl2 ) and lead acetate [Pb(CH3 COO)2 ] induces reproductive toxicity and development of testicular germ cell neoplasia in situ (GCNIS) in swiss albino mice. The effects of resveratrol to reverse the metal-induced toxicity were also analyzed. The mice were randomly divided into four groups for metal treatments and two groups received two different doses of each metal, CdCl2 (0.25 and 0.5 mg/kg) and Pb(CH3 COO)2 (3 and 6 mg/kg). The fourth group received oral doses of 20 mg/kg resveratrol in combination with 0.5 mg/kg CdCl2 or 6 mg/kg Pb(CH3 COO)2 for 16 weeks. Toxic effects of both metals were estimated qualitatively and quantitatively by the alterations in sperm parameters, oxidative stress markers, testicular histology, and protein expressions of the treated mice. Pronounced perturbation of sperm parameters, cellular redox balance were observed with severe distortion of testicular histo-architecture in metal exposed mice. Significant overexpression of Akt cascade and testicular GCNIS marker proteins were recorded in tissues treated with CdCl2 . Notable improvements were observed in all the evaluated parameters of resveratrol cotreated mice groups. Taken together, the findings of this study showed that long-term exposure to Cd and Pb compounds, induced acute reproductive toxicity and initiation of GCNIS development in mice. Conversely, resveratrol consumption abrogated metal-induced perturbation of spermatogenesis, testicular morphology, and the upregulation of Akt cascade proteins along with GCNIS markers, which could have induced the development of testicular cancer.

The effects of combined resveratrol and high intensity interval training on the hippocampus in aged male rats: An investigation into some signaling pathways related to mitochondria.

Objective(s):High-intensity interval training (HIIT) is a shape of interval training that provides ameliorated athletic capacity and has a good effect on health. Resveratrol is a natural polyphenol abundant in grapes and red wine and has been demonstrated to apply various useful health impacts on the body. This research aimed to evaluate the interactive effects of swimming HIIT and resveratrol consumption on SIRTs 3 & 4, NAD+/NADH, AMPK and SOD2 expression in aged rats.Materials and Methods:In total, forty-five old male albino rats (Wistar) with the age of twenty months were allocated into 5 groups randomly. Control group (Ctrl), Swimming HIIT group (Ex: Exercise), Swimming HIIT with Resveratrol consumption group (R+Ex), Resveratrol consumption group (R) and solvent of resveratrol consumption group (vehicle). R+Ex group accomplished the exercise and consumed resveratrol (10 mg/kg/day, gavage) for 6 weeks.Results:HIIT & resveratrol significantly increased NAD+/NADH, SOD 2 and AMPK in the aged rats. HIIT increased SIRT3, but resveratrol reduced it. As for SIRT4, HIIT decreased it, while resveratrol positively affected it.Conclusion:Resveratrol and HIIT, especially their combination, have anti-oxidant and anti-aging effects on the hippocampus of old rats.

The effect of 8 weeks of aerobic training and resveratrol consumption on the indicators of metabolic syndrome in overweight women

The effect of 8 weeks of aerobic training and resveratrol consumption on the indicators of metabolic syndrome in overweight women

Growth performance, immune response, antioxidant capacity and disease resistance against Yersinia ruckeri in rainbow trout ( Oncorhynchus mykiss ) as influenced through singular or combined consumption of resveratrol and two‐strain probiotics

In the current study, the possible synergistic properties of resveratrol (Res) and two-strain probiotics (Pro; Lactobacillus acidophilus and Bifidobacterium bifidum) on growth performance, innate immune responses, antioxidant defence and disease resistance against Yersinia ruckeri were assayed in rainbow trout. Accordingly, fish (10.42 ± 0.26 g) were fed with a basal diet as control and diets supplemented with R1 (400 mg/kg Res), R2 (800 mg/kg Res), P1 (0.5 g/kg Pro), P2 (1 g/kg Pro), R1P1 (400 mg/kg Res + 0.5 g/kg Pro) and R2P2 (800 mg/kg Res + 1g/kg Pro) for 8 weeks. The final weight, weight gain and feed conversion rate significantly improved in fish fed the P1, P2, R1P1 and R2P2 (p < .05), after 8 weeks. Diets containing R1P1 and R2P2 significantly elevated the total leucocyte count, and percentages of monocyte and neutrophil at the end of the 8th week (p < .05). Serum complement activity, lysozyme activity and bactericidal activity (BA) of fish fed the diets containing P1, P2, R1P1 and R2P2 were higher than the control group. Oral administration of R2P2 remarkably increased mucus immune parameters (lysozyme, BA, alkaline phosphatase and total immunoglobulin) (p < .05). Moreover, the highest activity of catalase, superoxide dismutase and glutathione peroxidase, and the survival rate were recorded in the R2P2 group. In conclusion, R2P2 remarkably elevated growth performance, innate immune and antioxidant responses, and disease resistance in rainbow trout.

Effect of resveratrol on C-reactive protein: An updated meta-analysis of randomized controlled trials.

We conducted a meta-analysis on the available randomized clinical trials (RCTs) to assess the role of resveratrol in lowering C-reactive protein (CRP) and high-sensitivity CRP (hs-CRP) levels, as markers of inflammation, in various inflammatory disorders. Literature search through Medline/PubMed, Scopus, ISI Web of Science, and Cochrane Library yielded 35 RCTs (24 studies for hs-CRP and 11 studies for CRP). Pooled results revealed that resveratrol supplementation significantly reduced the hs-CRP (MWD=-0.40 mg/L; 95% CI: -0.70 to -0.09 mg/L; p=.01) and CRP (MWD=-0.31 mg/L; 95% CI: -0.47 to -0.15 mg/L; p < .001) levels in serum. Subgroup analysis revealed that resveratrol in group with ≥10 weeks significantly reduces hs-CRP levels (MWD=-0.48 mg/L; 95% CI: -0.92 to -0.04 mg/L; p=.03) and CRP (WMD=-0.47 mg/L, 95% CI=-0.69 to -0.25, p < .001). A dose of ≥500 mg/day supplementation improves the levels of CRP, but not hs-CRP. This meta-analysis demonstrates that resveratrol consumption is effective in lowering the levels of CRP and hs-CRP in inflammatory conditions, especially if supplementation takes place for ≥10 weeks with ≥500 mg/day.

Effect of resveratrol on inflammatory cytokines: A meta-analysis of randomized controlled trials

The aim of the current study was to perform a meta-analysis of randomized clinical trials regarding the effect of resveratrol in decreasing the levels of inflammatory cytokines, including interleukin (IL)-1, IL-6, IL-8, and tumor necrosis factor (TNF)-α in a combination of inflammatory diseases. Literature search was carried out in Scopus, ISI web of science, Medline, and Cochrane Library databases by up to September 2020. The pooled effect size was determined through measuring the weighted mean differences (WMD) and their corresponding 95% confidence intervals (CI) for the difference between the resveratrol-receiving and control groups. Finally, 33 publications, including 3 studies on IL-1, 26 studies on IL-6, 4 studies on IL-8, and 21 studies on TNF-α met our final inclusion criteria and included in the quantitative analysis. Analysis in the overall population showed a significant effect of resveratrol consumption in reducing serum TNF-α levels (WMD = −0.66 pg/ml, 95% CI = −1.05 to −0.27, P = 0.001). A significant reduction of IL-6 concentration was observed only in the patients receiving ≥500 mg/day dose of resveratrol (WMD = −1.89 pg/ml, 95% CI = −3.73 to −0.05, P = 0.04) with inter-study heterogeneity (I2 = 94.4%, P < 0.001). Nonetheless, no significant alteration was observed in IL-1 (WMD = −0.14 pg/ml, 95% CI = −0.31 to 0.03, P = 0.10) and IL-8 (WMD = 0.18 pg/ml, 95% CI = −1.04 to 1.40, P = 0.73) levels following resveratrol consumption. Based on the present findings, resveratrol is able to decrease TNF-α and IL-6 (in ≥500 mg/day dose) levels but not IL-1 and IL-8 levels.

Effect of a Six Week-Swimming Interval Training with Resveratrol Consumption on Apoptotic Markers in the Liver Tissue of Aged Rat

Introduction: Aging involves a decrease in physiological function, physical activity and nutrition that modulate body functions. The aim of this study was to evaluate the effect of a six week- swimming interval training with resveratrol consumption on apoptotic markers in the liver tissue of aged rat. Methods: In this experimental study, thirty-two 20-month aged rats weighing 350-370 g were divided into four groups of 8 rats including 1) sham, 2) training, 3) resveratrol and 4) training + resveratrol. For six weeks, groups 3 and 4 received 100 mg/kg of resveratrol supplementation dissolved in 1% methylcellulose daily by gavage, and groups 2 and 4 performed swimming training three times a week. One-way analysis of variance with Tukey's post- hoc test was used to analyze the data (p &lt; 0.05). Results: Bcl2 Gene expression levels in the resveratrol and the exercise + resveratrol groups were significantly higher than the sham and exercise groups (p &lt; 0.05). Bax levels in the exercise + resveratrol group were lower than the resveratrol group, and the levels in the resveratrol group were higher than the sham group(p &lt;0.05).also Bax/Bcl2 levels in the exercise + resveratrol group were significantly lower than the exercise group(p &lt; 0.05). Conclusion: It seems that swimming interval training with resveratrol consumption has beneficial effects on anti-apoptotic markers, however, the effect of swimming interval training on liver apoptosis in the aging is still unknown and more studies are needed in this field.

Сравнительная оценка влияния ресвератрола и карнитина на полнотранскриптомный профиль ткани печени мышей с различной предрасположенностью к развитию алиментарного ожирения

Сравнительная оценка влияния ресвератрола и карнитина на полнотранскриптомный профиль ткани печени мышей с различной предрасположенностью к развитию алиментарного ожирения

Chronic resveratrol consumption prevents hypertension development altering electrophysiological currents and Ca2+ signaling in chromaffin cells from SHR rats

Resveratrol (RESV) is one of the most abundant polyphenol-stilbene compounds found in red wine with well-established cardioprotective and antihypertensive effects. Hyperactivity of the sympathoadrenal axis seems to be one of the major contributing factors in the pathogenesis of human essential hypertension. Alterations in outward voltage-dependent potassium currents (IK) and inward voltage-dependent sodium (INa), calcium (ICa) and nicotinic (IACh) currents, CCs excitability, Ca2+ homeostasis, and catecholamine exocytosis were previously related to the hypertensive state. This raised the issue of whether in vivo long-term RESV treatment can directly act as a modulator of Ca2+ influx or a regulator of ion channel permeability in CCs. We monitored outward and inward currents, and cytosolic Ca2+ concentrations ([Ca2+]c) using different pharmacological approaches in CCs from normotensive (WKY) and hypertensive (SHR) animals chronically exposed to trans-RESV (50 mg/L/v.o, 28 days). The long-term RESV treatment prevented the increase of the systolic blood pressure (SBP) in SHR, without reversion of cardiac hypertrophy. We also found an increase of the outward IK, reduction in inward INa,ICa, and IACh, and the mitigation of [Ca2+]c overload in CCs from SHR at the end of RESV treatment. Our data revealed that electrophysiological alterations of the CCs and in its Ca2+ homeostasis are potential new targets related to the antihypertensive effects of long-term RESV treatment.

Beverages Rich in Resveratrol and Physical Activity Attenuate Metabolic Changes Induced by High-Fat Diet

Consumption of saturated fat causes deleterious effects on health, which could be minimized through physical activity and foods with functional characteristics consumption. The aim of the study was to evaluate the beverage rich in resveratrol consumption and physical exercise in gut microbiota, body composition, lipid peroxidation, interleukin-6 (IL6) concentration and systolic blood pressure (SBP) of rats to the high-fat diet. Wistar rats were fed with control diet, high-fat diet (HFD), HFD and 15 mL solution of resveratrol, HFD and 15 mL of grape juice, HFD and 10 mL of red wine. All animals performed the physical training protocol five days a week. Grape juice and red wine composition were analyzed, SBP, body mass, consumption, adiposity and body composition, gut microbiota, lipid peroxidation and inflammation were evaluated. The grape juice (114.8 ± 22.5 mmHG) and red wine (129 ± 15.8 mmHg) groups showed lower SBP when compared to HFD (216.8 ± 20.6 mmHg) (p < 0.0001). The grape juice group (GJG) (39.1 ± 7) had a higher number of microbiota bands DNA when compared to the other groups (p = 0.002). The GJG (33.7 ± 6.7 pg/mL) presented lower concentration IL6 when compared to high-fat group (47.3 ± 16 pg/mL) (p = 0.003). GJG (4.7 ± 1.2 nmol/L) presented a lower concentration of TBARS when compared to control group (6.1 ± 1.4 nmol/L) and resveratrol group (6.6 ± 0.9 nmol/L), and the red wine group (7.4 ± 1.2 nmol/L) had a higher concentration of TBARS when compared to control group and GJG (p = 0.0001). The consumption of these beverages, especially grape juice, together with physical exercise, was able to promote beneficial changes even in the presence of a HFD.