Opportunities For Restoration Research Articles

Use of patent term extensions to restore regulatory time for medical devices in the United States

ABSTRACT Background Medical devices can seek patent term extensions (PTEs), which extend market exclusivity to compensate for delays related to clinical trials and regulatory review. Pharmaceutical companies commonly use PTEs, but their use by medical device companies has not been clear. Research Design and Methods We examined the use of PTEs by medical device companies between 1984 and 2024 using a database published in the Federal Register and a list published by the Patent and Trademark Office. Results Only 178 medical device submissions were linked to a PTE application. They were mostly concentrated in 116 product codes associated with 15 medical specialties; nearly half were associated with cardiovascular devices. Numbers increased significantly in the past decade. Successful applications restored 987 days on average. Conclusions The patent restoration opportunity appears underutilized. It is unclear whether some companies do not recognize the opportunity it promises, or whether it does not meet their needs. Different business features and marketing strategies in device versus pharmaceutical industries may decrease the usefulness of the PTE program for these types of medical products. However, the finding that a small subset of manufacturers operating in competitive markets adopted patent extension strategies more commonly suggests a significant competitive advantage when competition increases.

Enhancing iron biogeochemical cycling for canga ecosystem restoration: insights from microbial stimuli.

The microbial-induced restoration of ferruginous crusts (canga), which partially cover iron deposits and host unique ecosystems, is a promising alternative for reducing the environmental impacts of the iron mining industry. To investigate the potential of microbial action to accelerate the reduction and oxidation of iron in substrates rich in hematite and goethite, four different microbial treatments (water only as a control - W; culture medium only - MO; medium + microbial consortium - MI; medium + microbial consortium + soluble iron - MIC) were periodically applied to induce iron dissolution and subsequent precipitation. Except for W, all the treatments resulted in the formation of biocemented blocks. MO and MI treatments resulted in significant goethite dissolution, followed by precipitation of iron oxyhydroxides and an iron sulfate phase, due to iron oxidation, in addition to the preservation of microfossils. In the MIC treatment, biofilms were identified, but with few mineralogical changes in the iron-rich particles, indicating less iron cycling compared to the MO or MI treatment. Regarding microbial diversity, iron-reducing families, such as Enterobacteriaceae, were found in all microbially treated substrates. However, the presence of Bacillaceae indicates the importance of fermentative bacteria in accelerating the dissolution of iron minerals. The acceleration of iron cycling was also promoted by microorganisms that couple nitrate reduction with Fe(II) oxidation. These findings demonstrate a sustainable and streamlined opportunity for restoration in mining areas.

Decommissioning and restoration: early planning is critical to an efficient and risk-minimised process

Over the next 7 years, according to Wood Mackenzie, it is estimated that as many as 380 fields oil and gas facilities will be decommissioned across Asia–Pacific (Wood Mackenzie 2018). Many companies leave Decommissioning and Restoration (D&R) up to the respective project groups using their normal project management and control systems. However, managing D&R requires a different approach and mindset, as the value drivers and risk profiles for D&R projects are different compared to conventional construction projects. For D&R to be efficient – from operational, sustainability, regulatory compliance, environmental and financial perspectives – delivery must not be limited to an isolated project group. Rather, it must leverage corporate and operational knowledge, lessons learned from other decommissioning projects and industry best-practice to support the identification and management of the decommissioning risk profile and successful delivery thereof. Planning for D&R is essential at every stage of the life of an asset and should start as early as possible. Ideally, facilities are designed with D&R in mind at the project conception stage as the scope of D&R is often broader and more complex than operators realise, contributing to D&R projects often exceeding original cost estimates and timelines. Decommissioning and restoration opportunities may be missed or associated risks increased without timely recognition and management. In this paper, dss+ will provide companies with pragmatic guidance on how to plan and implement a D&R process that is managed well from an operational, risk, financial, contractor management and importantly, a stakeholder management point of view.

EFFECT OF PROBIOTIC TREATMENT ON THE MICROBIOLOGICAL ACTIVITY OF UKRAINIAN TYPICAL BLACK SOIL

The search for novel substances that promote the establishment of a microbial community and facilitate optimal humification processes while increasing soil organic matter content offers an opportunity for land restoration. The objective of study was to determine the abundance of ecological and trophic groups of soil microorganisms and the intensity of microbiological processes when subjected to probiotic treatment at various concentrations and doses in typical black soil. The eco-trophic groups of soil microorganisms were identified by inoculating dilutions of soil suspensions onto selective nutrient media. The direction and intensity of soil microbiological processes were assessed using the mineralization-immobilization, oligotrophy, and pedotrophy indexes. The results demonstrate that probiotics has a positive impact on the microbiological activity of the soil, leading to an increase in the number of ecological and trophic groups of soil microorganisms during both spring and autumn seasons. Notably, a significant effect on the soil microorganism conditions was observed after 30 days of probiotic treatment. Probiotics exhibit a favorable influence on microbiological processes within the soil, fostering conducive conditions for the development of soil microorganisms and the formation of humus. The most effective concentration of probiotics for promoting the functionality of microbial communities in black soils is determined to be 10%, accompanied by a dose of 100 l ha-1. Consequently, the application of probiotic treatment at a concentration of 10% and a dose of 100 l/ha-1 holds the potential to enhance the biological state of the soil, restore soil microbial diversity, and serve as an environmentally safe fertilizer.

Tracing agricultural land carbon loss through transregional food consumption for fairness of responsibility allocation

Agricultural land carbon loss (ALCL) has been increasingly triggered by transregional food consumption in China. Understanding land carbon burden from consumption side is then recognized to be essential for facilitating regional fairness. To this end, we combined physical food-orient multi-regional input-output table and ALCL inventory for 26 kinds of foods in 31 provinces to trace China's ALCL through cross-regional consumption. Results show that ALCL embodied in inter-provincial trade was up to 42 % of the national total ALCL. Food consumption in Guangdong caused the largest ALCL, while Tibet and Qinghai stood out from per capita perspective. Furthermore, consuming foods produced in areas with higher production ALCL intensity would elevate national ALCL, thus shrinking the adverse flows and controlling agricultural land expansion in high-intensity areas may help lessen national ALCL. This study contributes to equitable allocation of responsibility for land carbon loss, and enlightens future land carbon loss risks and restoration opportunities.

Survey of Connectivity Restoration in 3D Wireless Ad Hoc/Sensor Networks

Wireless ad hoc/sensor networks (WASN) have seen increased application in three-dimensional (3D) environments, such as underwater and aerial scenarios. However, WASN may be fragmented or unable to connect continuously due to the harsh surrounding environment or high mobility. Therefore, restoring the network connectivity and transmit data in real time is very important. This paper focuses on the critical task of restoring network connectivity in 3D WASN, a complex issue given that existing connectivity restoration algorithms for two-dimensional environments are not directly applicable or become overly complicated in 3D contexts. We present a comprehensive analysis of the current research landscape, summarizing key findings related to various aspects of 3D WASN connectivity restoration. These aspects include the application environment (underwater, in the sky, and recovery disaster), opportunities for restoration (active, passive, and active/passive), implementation strategies (clustering and sleep scheduling), and resource constraints (node deployment and movement control). Our study also proposes a classification of connectivity restoration solutions for 3D WASN, identifying existing gaps and suggesting potential future research directions. By providing specific insights and a structured overview of the field, we aim to contribute to the ongoing development of robust and resilient 3D WASN.

Variation in hazel dormouse (Muscardinus avellanarius) presence in hedge and scrub habitats

Variation in hazel dormouse (<i>Muscardinus avellanarius</i>) presence in hedge and scrub habitats

Including environmental and climatic considerations for sustainable coral reef restoration.

Coral reefs provide ecosystem benefits to millions of people but are threatened by rapid environmental change and ever-increasing human pressures. Restoration is becoming a priority strategy for coral reef conservation, yet implementation remains challenging and it is becoming increasingly apparent that indirect conservation and restoration approaches will not ensure the long-term sustainability of coral reefs. The important role of environmental conditions in restoration practice are currently undervalued, carrying substantial implications for restoration success. Giving paramount importance to environmental conditions, particularly during the pre-restoration planning phase, has the potential to bring about considerable improvements in coral reef restoration and innovation. This Essay argues that restoration risk may be reduced by adopting an environmentally aware perspective that gives historical, contemporary, and future context to restoration decisions. Such an approach will open up new restoration opportunities with improved sustainability that have the capacity to dynamically respond to environmental trajectories.

Climate change market-driven poleward shifts in cropland production create opportunities for tropical biodiversity conservation and habitat restoration

Although the impacts of climate change on the yields of crops have been studied, how these changes will result in the eventual realized crop production through market feedbacks has received little attention. Using a combination of attainable yield predictions for wheat, rice, maize, soybean and sugarcane, computable general equilibrium and land rent models, we project market impacts and crop-specific land-use change up to 2100 and the resulting implications for carbon and biodiversity. The results show a general increase in crop prices in tropical regions and a decrease in sub-tropical and temperate regions. Land-use change driven by market feedbacks generally amplify the effects of climate change on yields. Wheat, maize and sugarcane are projected to experience the most expansion especially in Canada and Russia, which also present the highest potential for habitat conversion-driven carbon emissions. Conversely, Latin America presents the highest extinction potential for birds, mammals and amphibians due to cropland expansion. Climate change is likely to redistribute agricultural production, generating market-driven land-use feedback effects which could, counterintuitively, protect global biodiversity by shifting global food production towards less-biodiverse temperate regions while creating substantial restoration opportunities in the tropics.

Conflicts and opportunities for commercial tree plantation expansion and biodiversity restoration across Brazil.

Substantial global restoration commitments are occurring alongside a rapid expansion in land-hungry tropical commodities, including to supply increasing demand for wood products. Future commercial tree plantations may deliver high timber yields, shrinking the footprint of production forestry, but there is an as-yet unquantified risk that plantations may expand into priority restoration areas, with marked environmental costs. Focusing on Brazil-a country of exceptional restoration importance and one of the largest tropical timber producers-we use random forest models and information on the economic, social, and spatial drivers of historic commercial tree plantation expansion to estimate and map the probability of future monoculture tree plantation expansion between 2020 and 2030. We then evaluate potential plantation-restoration conflicts and opportunities at national and biome-scales and under different future production and restoration pathways. Our simulations show that of 2.8 Mha of future plantation expansion (equivalent to plantation expansion 2010-2020), ~78,000 ha (3%) is forecast to occur in the top 1% of restoration priority areas for terrestrial vertebrates, with ~547,500 ha (20%) and ~1,300,000 ha (46%) in the top 10% and 30% of priority areas, respectively. Just ~459,000 ha (16%) of expansion is forecast within low-restoration areas (bottom 30% restoration priorities), and the first 1 Mha of plantation expansion is likely to have disproportionate impacts, with potential restoration-plantation overlap starkest in the Atlantic Forest but prominent in the Pampas and Cerrado as well. Our findings suggest that robust, coherent land-use policies must be deployed to ensure that significant trade-offs between restoration and production objectives are navigated, and that commodity expansion does not undermine the most tractable conservation gains under emerging global restoration agendas. They also highlight the potentially significant role an engaged forestry sector could play in improving biodiversity outcomes in restoration projects in Brazil, and presumably elsewhere.

Hydrogel-Based Therapy for Age-Related Macular Degeneration: Current Innovations, Impediments, and Future Perspectives.

Age-related macular degeneration (AMD) is an ocular disease that leads to progressive photoreceptor death and visual impairment. Currently, the most common therapeutic strategy is to deliver anti-vascular endothelial growth factor (anti-VEGF) agents into the eyes of patients with wet AMD. However, this treatment method requires repeated injections, which potentially results in surgical complications and unwanted side effects for patients. An effective therapeutic approach for dry AMD also remains elusive. Therefore, there is a surge of enthusiasm for the developing the biodegradable drug delivery systems with sustained release capability and develop a promising therapeutic strategy. Notably, the strides made in hydrogels which possess intricate three-dimensional polymer networks have profoundly facilitated the treatments of AMD. Researchers have established diverse hydrogel-based delivery systems with marvelous biocompatibility and efficacy. Advantageously, these hydrogel-based transplantation therapies provide promising opportunities for vision restoration. Herein, we provide an overview of the properties and potential of hydrogels for ocular delivery. We introduce recent advances in the utilization of hydrogels for the delivery of anti-VEGF and in cell implantation. Further refinements of these findings would lay the basis for developing more rational and curative therapies for AMD.

Wildlife and public perceptions of opportunities for psychological restoration in local natural settings

Abstract Wildlife might be important to psychologically restorative values and disvalues of nature, as interactions with wildlife could trigger both positive and negative feelings. Research on positive experiences of human–wildlife interactions has largely involved participants who voluntarily sought out wildlife experiences or it has addressed encounters with non‐threatening animals in urban green spaces. Less is known about the opportunities for psychological restoration in landscapes shared with mammals that are perceived to pose a threat to human activities and health. This study provides a nuanced understanding of the role of wildlife in public perceptions of the restorative potential and experience of psychological restoration in local natural settings. Twenty‐eight participants (15 women, 13 men, 18–75 years) took part in focus group interviews subject to a reflexive thematic analysis. As an analytical framework, we used a theoretical model for how people appraise the relevance, implications, coping potential and norm congruence of human–wildlife interactions and how such appraisals may support or hinder the restoration experienced in local natural settings. Relevance appraisals revealed shifts in consideration of the presence of wildlife from an integrated part of the natural scenery (background) to a distinct figure (foreground). Implication appraisals revealed that wildlife encounters would hinder the experienced psychological restoration if the animal was appraised as dangerous, disgusting, causing a nuisance or destructive. Wildlife encounters would promote restoration if the animal displayed attractive traits, features or fascinating behaviour or movements, and if it opened engaging interaction situations. Coping strategies perceived as feasible to deal with negative implications of wildlife involved avoidance of the local natural setting, preparatory behaviour displayed before a visit and precautionary behaviour displayed during the visit. Important public health effects might be gained if wildlife policy and management explicitly consider what animals mean to the perceived restorative potential of local natural settings. Read the free Plain Language Summary for this article on the Journal blog.

Quantifying the contributions of tributaries to large‐river fish populations through mark‐recapture modeling

AbstractObjectiveTributaries may play a vital role in maintaining populations of large river fishes, although the specific contributions of tributaries toward recruitment of river‐wide populations are not often understood. Tributaries may experience fewer cumulative anthropogenic impacts relative to main‐stem rivers and may offer more natural conditions supportive of native fish populations, which may provide opportunities for fish population restoration. Thus, an improved understanding of tributary–main‐stem population dynamics may inform targeted conservation actions for spatially structured populations of large‐river fishes. Colorado River tributaries in the Grand Canyon, Arizona, USA, are a focus of imperiled Humpback Chub Gila cypha conservation, which includes translocations to enhance population redundancy and to expand the overall population. However, the fate of fish dispersed to the main stem has not been thoroughly quantified.MethodsUsing open population mark–recapture models, we quantified the relative contribution of three groups of Humpback Chub, including fish of confirmed tributary origin that were either translocated or produced in situ, and others presumed to be Colorado River main‐stem‐origin fish, to three main‐stem populations. Our specific study objectives were to (1) estimate Colorado River abundances of tributary and main‐stem‐origin fish over time, (2) compare relative group‐specific contributions to three main‐stem populations, and (3) compare group‐specific survival rates of Humpback Chub in the Colorado River and in a tributary where a recent translocation has occurred.ResultTributaries contributed 26% and 43% of the overall abundance in two tributary inflow reach populations and zero in a third, which we attributed to uncharacteristically low tributary survival immediately following translocation. In the main stem, survival of tributary‐origin fish was higher compared to main‐stem‐origin fish, suggesting an advantage of tributary residence.ConclusionOur contrasting results from three different tributary inflow populations highlight the potential role for tributaries in sustaining large‐river fish populations, which may have important implications for long‐term maintenance of river metapopulations.

Advancing the optimization of urban–rural ecosystem service supply-demand mismatches and trade-offs

ContextIntensified human activities have disrupted landscape patterns, causing a reduction in the supply of ecosystem services (ESs) and an increase in demand, especially in urban agglomerations. This supply-demand imbalance will eventually lead to unsustainable landscapes and needs to be optimized.ObjectiveBased on ES supply-demand mismatch and trade-off relationships across urban–rural landscapes, this study explored which ESs need to be optimized and identified priority restoration regions of ESs that require optimization to promote landscape sustainability in Beijing-Tianjin-Hebei urban agglomeration.MethodsA methodological framework for ES supply-demand optimization in urban–rural landscapes was developed. urban–rural landscapes were identified using Iso cluster classification tool. ES supply was quantified using biophysical models and empirical formulas, and demand was quantified through consumption and expectations. Restoration Opportunities Optimization Tool was then adopted to identify priority regions.ResultsFrom 2000 to 2020, most of ES supply were lowest in urban areas and highest in rural areas, while demand exhibited the opposite. Although supply was increasing, it did not match demand. ES deficits were dominant in urban areas; both deficits and trade-offs were dominant in urban–rural fringe; and trade-offs were dominant in rural areas. There were 13,175 km2 of priority regions distributed in urban–rural landscapes, and their spatial heterogeneity was influenced by ES deficits and trade-offs.ConclusionDifferences in ESs supply-demand relationships affected the necessity of optimizing ESs zoning in urban–rural landscapes. Assigning weights reasonably according to trade-off curves to determine priority regions could facilitate both efficient use of resources and sustainable ES management for urban–rural regions.

From pixels to riverscapes: How remote sensing and geospatial tools can prioritize riverscape restoration at multiple scales

AbstractPrioritizing restoration opportunities effectively across entire riverscape networks (i.e., riverine landscape including floodplain and stream channel networks) can be difficult when relying on in‐channel, reach‐scale monitoring data, or watershed‐level summaries that fail to capture riverscape heterogeneity and the information necessary to implement restoration actions. Leveraging remote sensing and geospatial tools to develop spatially continuous information across nested hierarchical scales may support increased understanding of local riverscape reaches in their broader network context. Using riparian (vegetation) and geomorphic (elevation) indicators to assess status of riverscape health, along with a measure of restoration capacity (valley bottom area), could be adapted to fit specific management goals related to riverscape restoration. Frameworks using remotely sensed vegetation and elevation data to prioritize restoration continuously across riverscapes at restoration‐relevant, reach‐scales may uphold the ecosystem services provided by riverscapes. By incorporating local knowledge and identifying caveats for using these datasets, continuous inferences can be applied at network scales (watershed to regional extent and reach‐scale resolution) to prioritize restoration over a wide variety of ecoregions.This article is categorized under: Water and Life &gt; Conservation, Management, and Awareness Water and Life &gt; Methods Water and Life &gt; Stresses and Pressures on Ecosystems

Reviving Europe's rivers: Seven challenges in the implementation of the Nature Restoration Law to restore free‐flowing rivers

AbstractThe EU Nature Restoration Law represents an important opportunity for freshwater habitat restoration and, consequently, freshwater biodiversity protection. However, a number of challenges must be anticipated in its implementation, which may compromise its success. Some aspects, particularly those relating to freshwater ecosystems, require more clarification. We use riverine ecosystems to illustrate existing ambiguities in the proposed legislation and the potential consequences of leaving these aspects open to interpretation during the implementation process. We also discuss potential solutions to these problems which could help ensure that the law's objectives are met. We argue that river network structure and connectivity dimensions, which result into river meta‐ecosystems, must be explicitly considered. For that purpose, we ask for clear definitions of the critical terms “free‐flowing rivers,” “barriers,” and “reference areas.” In addition, we recommend developing methods for integrated assessment of connectivity across river networks. As a key property of river ecosystems, this must be used to prioritize actions to increase the length and number of free‐flowing rivers. Adequate restoration planning at larger spatial scales will benefit from a meta‐ecosystem perspective and accurate representation of aquatic‐terrestrial linkages, which will significantly improve the efficacy of restoration efforts. Furthermore, stakeholder and citizen engagement offer important opportunities at local, national, and European scales, and should be fostered to ensure inclusive decision‐making. The conservation challenges outlined here are particularly important for rivers, but they also have implications for other ecosystems. These considerations are useful for policymakers, conservationists, and other stakeholders involved in the Nature Restoration Law and related policy initiatives.This article is categorized under: Water and Life &gt; Stresses and Pressures on Ecosystems Water and Life &gt; Conservation, Management, and Awareness Human Water &gt; Water Governance

Effects of winter water quality conditions on Atlantic Salmon embryo mortality and deformity rates in the Serpentine River (New Brunswick, Canada)

In-stream incubation of Atlantic Salmon (Salmo salar) embryos within artificial redds using Jordan/Scotty fish egg incubator boxes is an alternative to traditional hatchery-based incubation methods. Winter water quality conditions, such as temperature and dissolved oxygen vary within natural redds and influence wild embryo development and survival. The differences in landscape characteristics and in-stream habitat features at catchment-, reach-, and site spatial scales were investigated to determine how they influenced water quality conditions within the Serpentine River, New Brunswick. A total of 14,400 hatchery-fertilized salmon embryos were incubated within river substrates from November 2020 to January 2021. The proportion of embryo mortalities within our experiment was high (44.9%), but still within the range observed in the literature from other incubation experiments, and the proportion of embryo deformities between our sites was very low (2.7%). Despite variations in average water temperature, cumulative degree days, and coefficient of variation in dissolved oxygen (DO) concentration, none were statistically significant for explaining embryo mortalities or deformities. Patterns were, however, evident across sites showing trends towards a decrease in mortality with cumulative degree days, and an increase in deformities with greater variation in DO. Improving our knowledge on Atlantic Salmon incubation habitat supports future research and restoration opportunities for adaptation under a changing climate.

The role of ecosystem restoration for a world in chaos—barriers and opportunities to optimize human wellbeing

There is an urgent need to globally advance human wellbeing and ecosystem restoration is required to achieve international targets. However, the relationship between ecosystem services and wellbeing is frequently assumed to be simple and positive, but this is not the case. This paper argues that a poor understanding of how and when ecosystem restoration can improve wellbeing causes a disconnect between the practice and the benefits it promises to provide. Problematic issues with carbon credits are discussed and a case is made against promoting ecosystem restoration initiatives based on carbon storage. Opportunities for ecosystem restoration to optimize gains in wellbeing are proposed, including the identification of sites where restoration has the greatest impact and the transition from carbon credit systems into ecosystem service credit systems. Future research directions are recommended, as are the production of international standards for ecosystem restoration in natural hazard recovery and risk mitigation.

Evaluating indicators of hydrologic alteration to demonstrate the impact of open-pit lignite mining on the flow regimes of small and medium-sized rivers

Our study provides a retrospective evaluation of the perturbances of the opencast mining on the small river. Based on a standard 'before-after-control-impact' approach, we assess the impact of disturbances on aquatic ecosystems. For this purpose, we used IHA (Index of Hydrological Alteration) statistics to fully quantify the impact of open-pit mine on flow regimes of small-to-medium-size rivers. The analysis results in flow data captured in the 33 parameters of the range variability approach (RVA) and five ecological flow components (EFC). This approach helped us comprehensively evaluate the alteration in the flow regime in areas of open-pit mining on the example of the Noteć River, a typical lowland river. We conclude that the level integrated hydrologic alterations to this river under the influence of the groundwater depression cone and water from the mine dewatering are high, as assessed by the RVA and EFC methods. Specifically, this work uses the IHA framework to verify the necessity of including a specific group of indicators. Our analysis shows that for the post-impact period on the investigated river, the functioning of the river ecosystem was degraded. One of the EFC components completely disappeared at both water gauge stations. There were also profound hydrologic alterations, such as increased frequency or duration of “extreme low flows” at one station; at the second station, “high flow pulses” occurred more frequently. More than 65 % of the altered parameters in five IHA groups were modified drastically. In the river section affected by the groundwater depression cone, both the minimum and maximum flows decreased by 20 % to 55 %. In the section where water from the mine dewatering is fed in, the minimum flows changed by + 170 to + 230 %. The use of the IHA tool provides an opportunity for environmental flow restoration of water conditions in degraded post-mining areas.

Avoid, adapt or exploit: Re-visiting bird responses to urbanization using a novel landscape approach

Urbanization threatens global biodiversity, yet numerous species persist in cities, highlighting opportunities for habitat restoration and conservation. To facilitate conservation planning, species frequently are assigned to urban tolerance groups such as ‘avoiders’, ‘adapters’ and ‘exploiters’. To be useful, species in such groups must show consistent responses to major gradients of change in urban landscapes. We used a landscape-scale approach to investigate the relative influence of housing cover and canopy tree cover on individual bird species and compared the results for species independently classified as urban avoiders, adapters or exploiters. We surveyed birds in 30 landscapes, positioned along concurrent gradients of housing and canopy tree cover, in residential areas of Melbourne, Australia. We modelled relationships between reporting rate for 42 species and the landscape cover of housing and canopy trees, for each gradient independently and in combination. Responses typically were non-linear and varied between species but generally were consistent within groups. Many species (∼40%) responded to a combination of both housing and canopy tree cover. Responses to housing typically were negative, while responses to tree cover were positive for avoiders and adapters, but negative for exploiters. Relationships with tree cover were stronger in landscapes with higher housing density. Whilst species responses likely represent a continuum, the relative consistency within and differences between urban tolerance groups affirm such general classifications to be a useful framework for synthesizing responses of multiple species to urbanization. Importantly, however, the status of at least four species changed considerably over the last 20 years, highlighting that such classifications are dynamic and respond to both environmental change in urban environments and changing biotic relationships.