Modification Of Response Research Articles

Developing Risk Assessment Items of Treatment Interruption Among Vietnamese Patients with Tuberculosis in Japanese DOTS—A Quantitative and Qualitative Survey Using the Delphi Method

Background: The number of foreign-born patients with tuberculosis (TB) has been increasing in Japan, and the number of Vietnamese patients was the highest in 2019. Tuberculosis (TB) is the second leading cause of death from infectious diseases after coronavirus disease-2019 (COVID-19). As the prevalence of TB varies widely globally, measures must be tailored to local characteristics. The Directly Observed Treatment (DOTS) short-course was introduced by the World Health Organization as a global strategy to overcome these challenges. The purpose of this study is to develop an original risk assessment for treatment interruption for Vietnamese patients with TB to be used in Directly Observed Treatment (DOTS), a tuberculosis control measure. Methods: The researchers adopted the Delphi method. Public health nurses of mid-career or above (n = 15) who had conducted DOTS for several Vietnamese patients with TB were selected and surveyed about the content and surface validities of the draft risk assessment items for treatment interruption. The survey was conducted three times. The quantification of content validity and the review and modification of responses regarding each item were analyzed by the researchers. Results: The results identified the following risk categories: “physical characteristics”, “background of life during residence”, “treatment environment”, “understanding tuberculosis and disease acceptance”, and “cultural and value trends”. Conclusions: The results suggest the need to provide support for tuberculosis recovery from various perspectives, including the living environment of Vietnamese patients with TB, their social environment in Japan, and the culture and values of their country of birth and upbringing.

Culturally responsive modifications to Gottman's Seven Principles Program for Arab American Adults: A feasibility study

AbstractObjectiveTo assess the feasibility of implementing a culturally responsive version of Gottman's Seven Principles relationship education program in a community‐based setting for married Arab American adults.BackgroundTo provide high‐quality couple and relationship education, it is necessary to acknowledge the population's needs and cultural backgrounds. One ethnic minority group that needs special attention is the Arab American community, which shares protective factors in terms of family support, religious beliefs, resiliency, and collectivist culture.MethodWe adapted Gottman's Seven Principles Program and conducted a qualitative feasibility study to examine recruitment capability, program acceptability, demand, and participant response among 16 Arab American married adults.ResultsThe results emphasize the vital role that thoughtful cultural and linguistic program adaptation played in increasing the acceptability and engagement of Arab American participants. However, results identified the ongoing stigma in Arab American communities against overtly seeking help for marital issues.ConclusionThis feasibility study provides helpful methodological and practical guidance to inform the design of larger scale randomized trials examining the efficacy of culturally responsive marriage education programs.ImplicationsThis study outlined actionable strategies, adaptations, venues, and communication channels to reduce barriers and optimize the delivery of marriage education to Arab American couples and families.

Investigating N6-methyladenosin RNA modification as a mediator of microRNA mechanical regulation in the heart

Abstract Background The heart is exposed to mechanical forces and continuously adapts to its environment in a process known as cardiac remodeling. Excessive mechanical load is a primary driver of pathological cardiac remodeling, leading to heart failure (HF), a prominent cause of morbidity and mortality worldwide. A set of microRNAs (miRs), termed mechano-miRs, has been identified to respond to mechanical forces and contribute to heart pathophysiology and HF. N6-methyladenosine (m6A) RNA modification is emerging as a novel regulatory layer impacting gene expression and occurring both in coding and non-coding RNAs. In this context, the N6-adenosine-methyltransferase METTL-3 regulates the processing of miR primary transcripts (pri-miRs) to functional miRs, promoting angiogenesis and cardiac repair after myocardial infarction. On the other side, dysregulation of m6A due to a defect in FTO, a m6A demethylase, has been implicated in HF. However, the role of m6A RNA modifications in heart mechanical responses and mechano-miR regulation remains unexplored. Purpose This project aims to: 1) investigate the impact of mechanical overload on the expression of m6A regulatory machinery components; 2) unveil the role of m6A-mechano-miRs axis in mechanically induced responses driving pathological cardiac remodeling leading to heart failure. Methods and Results Using living myocardial slices (LMS) technology, we modulate mechanical load in a multicellular environment while maintaining physiological behavior. Human LMS, obtained from the left ventricle of human donor non-failing hearts (NHS Blood and Transplant INOAR program, IRAS project ID: 189069) and rat LMS are subjected to two degrees of mechanical preload: physiological sarcomere length (SL) (SL = 2.2 μm) and overload (SL = 2.4 μm). This enables us to mimic the effects of mechanical stress on the heart during HF (volume overload model). LMS are maintained for 48 hours under electrical stimulation in circulating oxygenated media at 37°C. Mechanically overloaded LMS exhibit decreased contractility, accompanied by an increase in global m6A levels compared to physiological controls. Consistent with this finding, the analysis of the expression of m6A machinery reveals a downregulation of m6A demethylases (FTO and ALKBH5) in overloaded LMS. We identified putative cardiac mechano-miRs by small-RNA sequencing in both human and rat LMS and next predicted potential m6A sites on their primary transcripts via SRAMP (sequence-based RNA adenosine methylation site predictor). Conclusions Mechanical overload affects global m6A levels and the expression of m6A demethylases in the heart. Ongoing analysis of the m6A profile on the identified mechano-miR primary transcripts and mRNA targets holds promise for unveiling novel mechanically regulated m6A events, providing insights for transformative therapeutic strategies.

Integration of ubiquitination-related genes in predictive signatures for prognosis and immunotherapy response in sarcoma.

Ubiquitination is one of the most prevalent and complex post-translational modifications of proteins in eukaryotes, playing a critical role in regulating various physiological and pathological processes. Targeting ubiquitination pathways, either through inhibition or activation, holds promise as a novel therapeutic approach for cancer treatment. However, the expression patterns, prognostic significance, and underlying mechanisms of ubiquitination-related genes (URGs) in sarcoma (SARC) remain unclear. We analyzed URG expression patterns and prognostic implications in TCGA-SARC using public databases, identifying DEGs related to ubiquitination among SARC molecular subtypes. Functional enrichment analysis elucidated their biological significance. Prognostic signatures were developed using LASSO-Cox regression, and a predictive nomogram was constructed. External validation was performed using GEO datasets and clinical tissue samples. The association between URG risk scores and various clinical parameters, immune response, drug sensitivity, and RNA modification regulators was investigated. Integration of data from multiple sources and RT-qPCR confirmed upregulated expression of prognostic URGs in SARC. Single-cell RNA sequencing data analyzed URG distribution across immune cell types. Prediction analysis identified potential target genes of microRNAs and long non-coding RNAs. We identified five valuable genes (CALR, CASP3, BCL10, PSMD7, PSMD10) and constructed a prognostic model, simultaneously identifying two URG-related subtypes in SARC. The UEGs between subtypes in SARC are mainly enriched in pathways such as Cell cycle, focal adhesion, and ECM-receptor interaction. Analysis of URG risk scores reveals that patients with a low-risk score have better prognoses compared to those with high-risk scores. There is a significant correlation between DRG riskscore and clinical features, immune therapy response, drug sensitivity, and genes related to pan-RNA epigenetic modifications. High-risk SARC patients were identified as potential beneficiaries of immune checkpoint inhibitor therapy. We established regulatory axes in SARC, including CALR/hsa-miR-29c-3p/LINC00943, CASP3/hsa-miR-143-3p/LINC00944, and MIR503HG. RT-qPCR data further confirmed the upregulation of prognostic URGs in SARC. Finally, we validated the prognostic model's excellent predictive performance in predicting outcomes for SARC patients. We discovered a significant correlation between aberrant expression of URGs and prognosis in SARC patients, identifying a prognostic model related to ubiquitination. This model provides a basis for individualized treatment and immunotherapy decisions for SARC patients.

The impact of changes in water–sediment relationships at river confluences on the evolution of river bars

The confluence of tributaries and the main stream affects riverbed siltation and alters the upstream water–sediment relationships and flow structure of the main stream by adding additional flow. The relationship between these changes and the evolution of river bars, however, is yet to be fully understood. In this study, the areas of the river bars were extracted from Landsat image and analyzed using soft clustering to identify evolutionary patterns of the bars at the confluence of the Fen River (FR) and Yellow River (YR), and to analyze the hydrodynamic mechanism with hydrodynamic modeling. The results show that the spatial and temporal evolution of river bars in the study area over the past 50 years exhibits an evolutionary pattern, which exhibited evident clustering distributions and significant stage-based characteristics. This pertains to the water–sediment relationships as well as hydrodynamic fluctuations. At the confluence of FR and YR, the intensity of fluvial erosion experiences a mean increment of 9.67 %, while the incoming sediment coefficient witnesses a mean reduction of 5.74 %. The position of the confluence point exhibits a close association with the evolutionary characterized of spatial clustering and temporal phasing of the river bars. The river confluence area showcases a complex turbulence structure, wherein alterations in sediment transport capacity occur due to the influence of secondary flow and the topography of the confluence area. Consequently, this impacts the flushing of river sandbars and brings about modifications in siltation. Overall, this study provides the scientific basis for YR sediment management and channel modification in response to changing runoff and sediment conditions.

N6-Methyladenosine RNA Modification Regulates Maize Resistance to Maize Chlorotic Mottle Virus Infection.

Maize chlorotic mottle virus (MCMV) is one of the main viruses causing significant losses in maize. N6-methyladenosine (m6A) RNA modification has been proven to play important regulatory roles in plant development and stress response. In this study, we found that MCMV infection significantly up-regulated the m6A level in maize, and methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were performed to investigate the distribution of m6A modified peaks and gene expression patterns in MCMV-infected maize plants. The results showed that 1325 differentially methylated genes (DMGs) and 47 differentially methylated and expressed genes (DMEGs) were identified and analyzed. Moreover, the results of virus-induced gene silencing (VIGS) assays showed that ZmECT18 and ZmGST31 were required for MCMV infection, while silencing of ZmMTC, ZmSCI1 or ZmTIP1 significantly promoted MCMV infection in maize. Our findings provided novel insights into the regulatory roles of m6A modification in maize response to MCMV infection.

Circadian cilia transcriptome in mouse brain across physiological and pathological states

Primary cilia are dynamic sensory organelles that continuously undergo structural modifications in response to environmental and cellular signals, many of which exhibit rhythmic patterns. Building on our previous findings of rhythmic cilia-related gene expression in diurnal primates (baboon), this study extends the investigation to the nocturnal mouse brain to identify circadian patterns of cilia gene expression across brain regions. We used computational techniques and transcriptomic data from four publicly available databases, to examine the circadian expression of cilia-associated genes within six brain areas: brainstem, cerebellum, hippocampus, hypothalamus, striatum, and suprachiasmatic nucleus. Our analysis reveals that a substantial proportion of cilia transcripts exhibit circadian rhythmicity across the examined regions, with notable overrepresentation in the striatum, hippocampus, and cerebellum. We also demonstrate region-specific variations in the abundance and timing of circadian cilia genes’ peaks, indicating an adaptation to the distinct physiological roles of each brain region. Additionally, we show that the rhythmic patterns of cilia transcripts are shifted under various physiological and pathological conditions, including modulation of the dopamine system, high-fat diet, and epileptic conditions, indicating the adaptable nature of cilia transcripts’ oscillation. While limited to a few mouse brain regions, our study provides initial insights into the distinct circadian patterns of cilia transcripts and highlights the need for future research to expand the mapping across wider brain areas to fully understand the role of cilia’s spatiotemporal dynamics in brain functions.

Enhancing operational performance assessment of structures with seismic response modification devices: The role of observability and symmetry analysis under limited sensor deployment

AbstractTo manage structural responses under various external forces, the increasing incorporation of seismic isolation and supplementary damping systems in modern civil engineering necessitates post‐installation performance assessments. The challenge of accurately inferring system information from these complex dynamical structures, especially with limited sensor deployment, could be significant. From the perspective of solving inverse problems, this challenge hinges on constructing an input‐output mapping that assures unique solutions, achievable through theoretical observability or symmetry analysis. We introduce a unified algorithm framework designed to accommodate various definitions of Lie derivatives, specifically for observability and symmetry analysis in dynamic systems with affine, non‐affine, and unknown inputs—capabilities not fully achieved in previous studies. We demonstrate its application across typical dynamic scenarios, including both linear and nonlinear examples. We also present a numerical example featuring complex isolation systems with limited sensor layouts, illustrating how uniform convergence can be achieved in estimating all system states when an observable input‐output mapping is utilized. Furthermore, an experimental example employing shaking table tests showcases the potential complications that arise when an unobservable input‐output mapping is used.

Exploring changes in social spider DNA methylation profiles in all cytosine contexts following infection.

Living at high density and with low genetic diversity are factors that should both increase the susceptibility of organisms to disease. Therefore, group living organisms, especially those that are inbred, should be especially vulnerable to infection and therefore have particular strategies to cope with infection. Phenotypic plasticity, underpinned by epigenetic changes, could allow group living organisms to rapidly respond to infection challenges. To explore the potential role of epigenetic modifications in the immune response to a group-living species with low genetic diversity, we compared the genome-wide DNA methylation profiles of five colonies of social spiders (Stegodyphus dumicola) in their natural habitat in Namibia at the point just before they succumbed to infection to a point at least six months previously where they were presumably healthier. We found increases in genome- and chromosome-wide methylation levels in the CpG, CHG, and CHH contexts, although the genome-wide changes were not clearly different from zero. These changes were most prominent in the CHG context, especially at a narrow region of chromosome 13, hinting at an as-of-yet unsuspected role of this DNA methylation context in phenotypic plasticity. However, there were few clear patterns of differential methylation at the base level, and genes with a known immune function in spiders had mean methylation changes close to zero. Our results suggest that DNA methylation may change with infection at large genomic scales, but that this type of epigenetic change is not necessarily integral to the immune response of social spiders.

Seismic design parameters of double-layer diamatic domes

The effect of past earthquakes (e.g., the 1995 Kobe and 2016 Kumamoto earthquakes) on the space structures revealed the fact that the assumption of the invulnerability of these structures is incorrect despite their light self-weight and high degree of redundancy, and space structures may suffer severe damage during strong ground motions. Therefore, special attention should be paid to the design of this type of structure in high seismic-risk regions. Nevertheless, the existing seismic design codes have not provided the seismic design parameters (i.e., the seismic response modification factors), including the behavior factor, R, and the displacement amplification factor, Cd for space structures. This research aims to extract the seismic design parameters of double-layer diamatic domes (DLDDs) to answer the challenges that structural engineers face in the design of this type of structure. For this purpose, 16 DLDD models with different rise-to-span ratios (RSRs) and span lengths were considered. After designing the models and performing the nonlinear time history analysis using the simultaneous effect of the three transitional components of ground motions, the target displacement at the control node was determined. Then, the pushover analysis was carried out to derive the capacity curve of the structures and calculate the seismic response modification factors in both horizontal and vertical directions. The results indicate that the domes with RSRs of 1/7 and 1/5 should be designed such that they remain in the elastic state in the horizontal direction. Then, with the growth of RSR, the value of R increases and reaches 1.792. Finally, equations were presented in this study for the period, behavior factor, and displacement amplification factor of DLDD structures in both horizontal and vertical directions using the nonlinear regression analysis.

Parametric evaluation of the response modification factor R considering bidirectional ground motions

The current research on evaluating the response modification factor R, related to the lateral strength demand of structures, has been generally based on inelastic single-degree-of-freedom systems. Nevertheless, most structures have more than one main analysis component and will be subjected to bidirectional ground motions. In this study, the results of the parametric evaluation of the response modification factor considering the bidirectional interaction (Rb) of inelastic two-degree-of-freedom systems (2DOF) are presented. The effects on the factor Rb of the vibration period, the ductility capacity, the hysteretic model, the seismic incidence angle, and the period ratio were evaluated. Analysis results show that the bidirectional interaction could increase the lateral strength demand of the 2DOF systems because of the coupling effect of the two components’ responses. To make this research useful for improving engineering practice and code provisions, the main contribution is the proposal of a simplified expression for estimating the factor Rb.

Testing of battery separators: transducers and methods for air-coupled ultrasonic sensing.

Abstract Battery separator me3mbranes are thin polymeric microporous films placed between battery electrodes. They are a key component that strongly affects both battery performance and security and controlling its main properties is critical for the industry. In particular, the manufacturing process demands tests that allow checking both the mechanical integrity (porosity, thickness, pore size, etc.) of the materials and the consistency of these properties during the construction of the batteries. The use of an air-coupled ultrasonic system is proposed in this work. It is expected that this will make possible the non-destructive and quick testing of these materials, in order to evaluate thickness, stiffness, pore distribution and pore size of the separator. By means of three pairs of transducers, the modulus and phase of the transmission coefficient was measured from 0.35 to 1.4 MHz in different materials. The measurements show a clear modification of the film response produced by the presence of porosity. This suggests that the transmission of ultrasonic waves in battery separators using air-coupled ultrasound may have two contributions: one corresponds to the propagation though the solid part, the other corresponds to propagation in the pores. The former allows to determine properties like film thickness and elastic constants, while the latter allows to determine porosity and pore size and pore tortuosity. This work set the basis for the development of a quality test for the in-line inspection of these materials during their fabrication. The observed ultrasonic response in microporous separators suggest the possibility to use low frequency air-coupled transducers with limited sensitivity, where the use of MEM transducers at industrial scale can be advantageous.

Apoptotic Cell-Based Therapy for the Modification of the Inflammatory Response to Hemorrhagic Shock.

Many trauma patients die from hemorrhagic shock in the military and civilian settings. Although two-thirds of hemorrhagic shock victims die of reasons other than exsanguination, such as the consequent cytokine storm, anti-inflammatory therapies failed to be utilized. Apoptotic cell-based treatments enhance innate ability to exert systemic immunomodulation as demonstrated in several clinical applications and hence might present a novel approach in hemorrhagic shock treatment. Twenty-two rats underwent a pressure-controlled hemorrhagic shock model and followed up for 24 hours. An infusion of apoptotic cells (Allocetra-OTS, Enlivex Therapeutics Ltd, Nes Ziona, Israel) was administered to the treatment group. Hemodynamics, blood counts, biochemistry findings, and cytokine profile were compared to a saline-resuscitated control group. The treatment group's mean arterial pressure decreased from 94.8 mmHg to 28.2 mmHg, resulting in an 8.13 mg/dL increase in lactate and a 1.9 g/L decrease in hemoglobin, similar to the control group. White blood cells and platelets decreased more profoundly in the treatment group. A similar cytokine profile after 24 hours was markedly attenuated in the treatment group 2 hours after bleeding. Levels of pro-inflammatory cytokines such as interleukin (IL)-1a (28.4 pg/mL vs. 179.1 pg/mL), IL-1b (47.4 pg/mL vs. 103.9 pg/mL), IL-6 (526.2 pg/mL vs. 3492 pg/mL), interferon γ (11.4 pg/mL vs. 427.9 pg/mL), and tumor necrosis factor α (19.0 pg/mL vs. 31.7 pg/mL) were profoundly lower in the treatment group. In a pressure-control hemorrhagic shock model in rats, apoptotic cell infusion showed preliminary signs of a uniform attenuated cytokine response. Apoptotic cell-based therapies might serve as a novel immunomodulatory therapy for hemorrhagic shock.

Cell Wall Profiling of the Resurrection Plants Craterostigma plantagineum and Lindernia brevidens and Their Desiccation-Sensitive Relative, Lindernia subracemosa.

Vegetative desiccation tolerance has evolved within the genera Craterostigma and Lindernia. A centre of endemism and diversification for these plants appears to occur in ancient tropical montane rainforests of east Africa in Kenya and Tanzania. Lindernia subracemosa, a desiccation-sensitive relative of Craterostigma plantagineum, occurs in these rainforests and experiences adequate rainfall and thus does not require desiccation tolerance. However, sharing this inselberg habitat, another species, Lindernia brevidens, does retain vegetative desiccation tolerance and is also related to the resurrection plant C. plantagineum found in South Africa. Leaf material was collected from all three species at different stages of hydration: fully hydrated (ca. 90% relative water content), half-dry (ca. 45% relative water content) and fully desiccated (ca. 5% relative water content). Cell wall monosaccharide datasets were collected from all three species. Comprehensive microarray polymer profiling (CoMPP) was performed using ca. 27 plant cell-wall-specific antibodies and carbohydrate-binding module probes. Some differences in pectin, xyloglucan and extension epitopes were observed between the selected species. Overall, cell wall compositions were similar, suggesting that wall modifications in response to vegetative desiccation involve subtle cell wall remodelling that is not reflected by the compositional analysis and that the plants and their walls are constitutively protected against desiccation.

Concept and role of immunomodulatory medicinal plants used in Ayurvedic health care system

The statement “prevention is better than cure” and its applications is not a new for Ayurvedic health care system. The statement defines the aim and objectives of Ayurvedic health care system in a nutshell “swasthasya swasthya rakshanam aaturasya vikara prashamanam”. The immune system is certainly considered one of our most discreet biological systems withinside the body. Immunizations may be obtained by us actively or passively. Active immunization includes stimulating with an antigen to generate immunological defenses against a destiny exposure, rather than passive vaccination, which includes administering antibodies to someone who has already been exposed to an antigen. Both plant and animal sources produce immunomodulatory effects to boost the body’s immunological reactivity against infections by engaging the non-specific immune system. Herbal immunomodulators are substances that either activate or suppress innate and adaptive immune responses in the body. Different ailments including allergies, asthma, rheumatoid arthritis, cancer, and other infectious disorders are caused by immune system failure. Therefore, regulating the many infectious illnesses requires significant immune response modification. Global scientific study is currently focused on how various medicinal plant components might alter the immune system. Numerous Indian and “Rasayana” and medicinal plants may have immunomodulatory characteristics. Some of these plants are Tinospora cordifolia, Morus alba, Acacia catechu, Allium sativum, and Mangifera indica. There are many more that are still undiscovered and offer space for greater study.

Modifying Factors of the Incidence of Type 1 Diabetes Mellitus in Children under 18 Years of Age in Northern Spain in the Last 3 Decades

Introduction: Type 1 Diabetes Mellitus is a chronic disease of autoimmune etiology that is highly prevalent in childhood. In the last decade, the possibility of the influence of the Bacillus Calmette-Guérin vaccine, different autoimmune diseases, modification of the immune response, and COVID19 itself of a simple population change due to immigration. Objectives: The objective of this work is to study the incidence of Type 1 Diabetes Mellitus in a cohort of children from Euskadi, north of Spain in the last 15 years. Methods: For the epidemiological study in Euskadi, a comparison of accumulated incidences between vaccinated and unvaccinated population groups was made. Results: The results of our study were not conclusive, but we believe that it is necessary to continue with long-term studies to eliminate bias.

Interplay between DOT1L and HDAC1 regulates Leishmania donovani infection in human THP-1 cells

Leishmania donovani, a protozoan parasite, causes visceral leishmaniasis. The parasite modifies the global gene expressions of the host genome, facilitating its survival within the host. Thus, the host epigenetic modulators play important roles in host-pathogen interaction and host epigenetic modification in response to infection. Previously, we had reported that the host epigenetic modulator, histone deacetylase 1 (HDAC1) expression was upregulated on Leishmania donovani infection. This upregulation led to the repression of host defensin genes in response to the infection. In this paper, we have investigated the interplay between the host DOT1L, a histone methyltransferase, and HDAC1 in response to Leishmania donovani infection. We show that the expression of DOT1L is upregulated both at transcript and protein level following infection leading to increase in H3K79me, H3K79me2, and H3K79me3 levels. ChIP experiments showed that DOT1L regulated the expression of HDAC1. Downregulation of DOT1L using siRNA resulted in decreased expression of HDAC1 and increased transcription of defensin genes and thereby, lower parasite load. In turn, HDAC1 regulates the expression of DOT1L on Leishmania donovani infection as downregulation of HDAC1 using siRNA led to reduced expression of DOT1L. Thus, during Leishmania donovani infection, an interplay between DOT1L and HDAC1 regulates the expression of these two histone modifiers leading to downregulation of defensin gene expression.

How Histone Acetyltransferases Shape Plant Photomorphogenesis and UV Response.

Higher plants have developed complex mechanisms to adapt to fluctuating environmental conditions with light playing a vital role in photosynthesis and influencing various developmental processes, including photomorphogenesis. Exposure to ultraviolet (UV) radiation can cause cellular damage, necessitating effective DNA repair mechanisms. Histone acetyltransferases (HATs) play a crucial role in regulating chromatin structure and gene expression, thereby contributing to the repair mechanisms. HATs facilitate chromatin relaxation, enabling transcriptional activation necessary for plant development and stress responses. The intricate relationship between HATs, light signaling pathways and chromatin dynamics has been increasingly understood, providing valuable insights into plant adaptability. This review explores the role of HATs in plant photomorphogenesis, chromatin remodeling and gene regulation, highlighting the importance of chromatin modifications in plant responses to light and various stressors. It emphasizes the need for further research on individual HAT family members and their interactions with other epigenetic factors. Advanced genomic approaches and genome-editing technologies offer promising avenues for enhancing crop resilience and productivity through targeted manipulation of HAT activities. Understanding these mechanisms is essential for developing strategies to improve plant growth and stress tolerance, contributing to sustainable agriculture in the face of a changing climate.

Defining context-dependent m6A RNA methylomes in Arabidopsis

N6-Methyladenosine (m6A) prevalently occurs on cellular RNA across almost all kingdoms of life. It governs RNA fate and is essential for development and stress responses. However, the dynamic, context-dependent m6A methylomes across tissues and in response to various stimuli remain largely unknown in multicellular organisms. Here, we generate a comprehensive census that identifies m6A methylomes in 100 samples during development or following exposure to various external conditions in Arabidopsis thaliana. We demonstrate that m6A is a suitable biomarker to reflect the developmental lineage, and that various stimuli rapidly affect m6A methylomes that constitute the regulatory network required for an effective response to the stimuli. Integrative analyses of the census and its correlation with m6A regulators identify multiple layers of regulation on highly context-dependent m6A modification in response to diverse developmental and environmental stimuli, providing insights into m6A modification dynamics in the myriad contexts of multicellular organisms.

Regulation of oxidative stress response and antioxidant modification in Corynebacterium glutamicum.

As an efficient and safe industrial bacterium, Corynebacterium glutamicum has extensive application in amino acid production. However, it often faces oxidative stress induced by reactive oxygen species (ROS), leading to diminished production efficiency. To enhance the robustness of C. glutamicum, numerous studies have focused on elucidating its regulatory mechanisms under various stress conditions such as heat, acid, and sulfur stress. However, a comprehensive review of its defense mechanisms against oxidative stress is needed. This review offers an in-depth overview of the mechanisms C. glutamicum employs to manage oxidative stress. It covers both enzymatic and non-enzymatic systems, including antioxidant enzymes, regulatory protein families, sigma factors involved in transcription, and physiological redox reduction pathways. This review provides insights for advancing research on the antioxidant mechanisms of C. glutamicum and sheds light on its potential applications in industrial production.