To assess a commercially available electronic whiteboard's usability and acceptability in isolation rooms, focusing on improving nurse-patient communication and supporting data input. A cross-sectional study with quantitative and qualitative mixed methods. We evaluated the usability and acceptability of electronic whiteboards among nurses using scenarios in a virtual isolation room environment. Nurses recognised the electronic whiteboard as a valuable tool for communication and error reductions in record-keeping but noted a learning curve for less tech-savvy users. Positive correlations were found between perceived usefulness, ease of use and adoption intent. Despite challenges, electronic whiteboards show promise for enhancing patient care, requiring comprehensive training and management systems. Time allocation in patient wards and nurse-patient interactions are crucial considerations. Electronic whiteboards have usability and acceptability as a tool to improve nurse-patient communication. However, considering technical issues and staff resistance, a management system and user training are necessary. Nurses perceive electronic whiteboards as user-friendly and as facilitating data input. TREND (Nonrandomised evaluations of behavioural and public health interventions). No patient or public contribution.